Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,380,469	C→T	E17K (GAA→AAA)	frdB ←	fumarate reductase iron‑sulfur protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,380,469	0	C	T	98.9%	295.6 / NA	92	E17K (GAA→AAA)	frdB	fumarate reductase iron‑sulfur protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (59/32);  minor base G (0/1);  total (59/33)
Fisher's exact test for biased strand distribution p-value = 3.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTG‑CCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGA  >  NC_000913/4380227‑4380714                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                        gAAGTTAGCTAACGCTTCAACCTTCATCCCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTGTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTtat                                                                                                                                                                                                                                                 <  2:341054/251‑1 (MQ=255)  taGTTAGCTAACGCTTCACCCTAAATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCAGAAGGAACCACAAATCGACATACGGCCGGCCCAGCGGTAGCTCAGGTCCGGAGCCAGGCTGTCTTGGATGTAAACCAGCGCATCCAGCAATGAGGGTGTTGCGGCCTAGGGCAATTCATAGTATGCGCTGTGCGGGGCGGTATCGACTTTCGGGGtatt                                                                                                                                                                                                                                                >  2:359067/2‑250 (MQ=255)     ttAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAgcg                                                                                                                                                                                                                                             >  1:12627/1‑251 (MQ=255)      tAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGGATCGACTTTCGGGTTATAgcgc                                                                                                                                                                                                                                            >  1:32643/1‑251 (MQ=255)       aGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGca                                                                                                                                                                                                                                           <  1:251752/251‑1 (MQ=255)            aCGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCac‑cacc                                                                                                                                                                                                                                     >  1:233503/1‑251 (MQ=255)                          aTACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGtt                                                                                                                                                                                                                       <  1:554182/251‑1 (MQ=255)                            accgtcggtgctatagcGCAGGAAGGTTTTACATGACAGTTTGGGCACGTTGTAAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGGCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGtttt                                                                                                                                                                                                                     <  2:134061/235‑1 (MQ=255)                             ccGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAAGAAACACAAATCGCCATACAGAAGGACCAACGGTAGCTCAGGACCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGAGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGttttt                                                                                                                                                                                                                    >  1:539117/1‑251 (MQ=255)                               gTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCa                                                                                                                                                                                                                  <  1:32883/251‑1 (MQ=255)                               gTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCCCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCCGCGCCTCCCGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGAGGGATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGttttta                                                                                                                                                                                                                   >  2:421635/1‑249 (MQ=255)                                    tgtAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCAtctc                                                                                                                                                                                                              >  1:494662/1‑250 (MQ=255)                                    tgtAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGACGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCAtctcc                                                                                                                                                                                                             >  1:44975/1‑250 (MQ=255)                                     gtAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCa                                                                                                                                                                                                             <  1:222407/250‑1 (MQ=255)                                      tAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCCAGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGAGCGGTATCGACTTTCGGGTTATAACG‑ACACAGCTCAATTTTCCGGTTTTTCAtctccgc                                                                                                                                                                                                           >  2:392882/1‑248 (MQ=255)                                      tAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGc                                                                                                                                                                                                           >  1:447216/1‑251 (MQ=255)                                       aaTCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGAAATGAGGGAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCGA‑CACCTCAATTTTATGGTTTTTCAACTAAGcc                                                                                                                                                                                                          >  2:489944/1‑251 (MQ=255)                                       aaTCACCCAGGATTGTGTTACAGGCCAGTGTTGGCACGGTGTTAACCATCATGCCGCAGTAACCACAAATCGCCATACTGCAGGTCCGGTGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGATGTAGTTGCGTCATAAGGCACTTCATAGAATTCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CATCTCAATTTTCAGGTTTTTCATCTCAGcc                                                                                                                                                                                                          <  2:341065/251‑1 (MQ=255)                                              cAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGcc                                                                                                                                                                                                   <  1:384717/251‑1 (MQ=255)                                              cAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGGGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGGAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGGTTTTCATCTCAGCCATTCGcc                                                                                                                                                                                                   >  2:77125/1‑251 (MQ=255)                                                 gAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCCTGCCGCCGGAACCACAAATCGCCATACGGCAGAAACAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGGTGCCTGCTAAAGCCCTTCATAGAATGCGCTATGCGGTGCGGTATCGCCTTTCGGTTTATAACGCAC‑CACCTCAATTTTCAGGATTTTCTTCTCAGCCCTTTGccttc                                                                                                                                                                                                >  2:519348/1‑251 (MQ=255)                                                      ttttACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGAGGTAGCTCAGGTCCGGTGACAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCCATTTTAAGGTTTTTCATCTCATCCATTCGccttctcctt                                                                                                                                                                                           >  2:66748/1‑251 (MQ=255)                                                         tACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAAGCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGccttctccttct                                                                                                                                                                                         <  2:233503/250‑1 (MQ=255)                                                          aCATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCcttctt                                                                                                                                                                                        <  1:256214/250‑1 (MQ=255)                                                           cATGCCAGTTGTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGACATACAGCAGGACCAGCGGTAGCTCAGGGCCGGTGCCAGGTTGGCTTTGATGTGACCCAGCGCATCCCGGAATGAGGTAGTTGCGTCATAAGGCCCTTCCAAGAATGCGCTATGCGGGGCGGTATCGCCTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGGTTTTCCTCTCTGCCCTTTGCCTTCTCCTTCTTAt                                                                                                                                                                                      >  2:48054/1‑251 (MQ=255)                                                              gacAGTTTTGGCACGTTGTTAACAATCATGCCGCAGGAACCTCAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGTATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCGTCTTATTgg                                                                                                                                                                                   <  2:489824/249‑1 (MQ=255)                                                               ccAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGgc                                                                                                                                                                                  >  1:359926/1‑251 (MQ=255)                                                                  gTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGGATCGACTTTCGGGTTATAGCGCCC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGgctgc                                                                                                                                                                               >  2:283813/1‑251 (MQ=255)                                                                      tGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTcc                                                                                                                                                                           >  1:515875/1‑251 (MQ=255)                                                                           cGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGGCTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCCGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCCCCtt                                                                                                                                                                      >  2:155109/1‑251 (MQ=255)                                                                           cGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCtt                                                                                                                                                                      <  1:711/251‑1 (MQ=255)                                                                                      atcatGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCa                                                                                                                                                                                                                  >  1:11250/1‑196 (MQ=255)                                                                                       tcatGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCCGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCCCTTCATAGAATGCGCTATGCGGAGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTTTTGGCTGCTTCCCCCTTTTCGGCTGcctc                                                                                                                                                          >  2:143382/1‑248 (MQ=255)                                                                                       tcatGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATc                                                                                                                                                          >  1:118242/1‑251 (MQ=255)                                                                                        catGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATcc                                                                                                                                                         <  1:284237/251‑1 (MQ=255)                                                                                               cAGGAACCACAAATCGCCATACGGCAGGACCAGCGGCAGCTCAGGTACGGTGCCAGGTTGACTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGc                                                                                                                                                  >  1:506106/1‑251 (MQ=255)                                                                                                aGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGcc                                                                                                                                                 <  1:35976/251‑1 (MQ=255)                                                                                                  gAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGATGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGAAGGTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTTTGGGCTGCATCCCCTTCGCCAc                                                                                                                                               >  2:182711/1‑251 (MQ=255)                                                                                                         aaaTCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAAc                                                                                                                                        >  1:559219/1‑251 (MQ=255)                                                                                                                    cGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTg                                                                                                                             <  1:477451/251‑1 (MQ=255)                                                                                                                       cAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTggcg                                                                                                                          >  1:465676/1‑251 (MQ=255)                                                                                                                           aCCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCCTACGCTTCGCCACCGTAAACGCGTTTAGCTggcggc                                                                                                                        >  2:476986/1‑249 (MQ=255)                                                                                                                            ccAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGc                                                                                                                     >  1:140210/1‑251 (MQ=255)                                                                                                                              aGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCgg                                                                                                                   >  1:35091/1‑250 (MQ=255)                                                                                                                              aGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTGGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCgt                                                                                                                   >  1:223895/1‑251 (MQ=255)                                                                                                                                cGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCgtag                                                                                                                 <  1:340663/251‑1 (MQ=255)                                                                                                                                 ggTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCgtagt                                                                                                                >  1:488659/1‑251 (MQ=255)                                                                                                                                         aGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTcc                                                                                                        >  1:192451/1‑250 (MQ=255)                                                                                                                                           gTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACg                                                                                                      <  1:140176/251‑1 (MQ=255)                                                                                                                                             tcggtacaaggctGTCTTTGATGTAGCCCACCGCATCCAGTAATGCGGTCGTTGCGTCATATGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGTTGCTTCCGCCTTATCGGCTTCATCCGACTCGCCACCGTAAACGCGTTTAGCTGGCGGCCGAGTGGTAATCTTCCCGTc                                                                                                    >  2:29031/13‑251 (MQ=255)                                                                                                                                                    cAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCACAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCTCCTTATCGGCTGAATCCGCTTCTCCACCGTCAACGCGTTTAGCTGGCGGCAGCGTAGTCATCTTCACGTCGCTggac                                                                                             >  2:166144/1‑248 (MQ=255)                                                                                                                                                      ggTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTc                                                                                           <  1:182711/251‑1 (MQ=255)                                                                                                                                                       gTTGGCTTTGATGGAGCCCAGCGCATCCAGTAATGAGGTAGTGGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTcc                                                                                          <  2:559219/251‑1 (MQ=255)                                                                                                                                                        ttGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTACACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTccc                                                                                         >  1:360161/1‑250 (MQ=255)                                                                                                                                                        ttGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAATCGCGATTAGCTGGCGGCCGCGTAGTAATCTTCACGTCGCTGTACTccc                                                                                         >  2:113764/1‑250 (MQ=255)                                                                                                                                                          gTCTCTGATGGAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAgg                                                                                       <  2:12627/251‑1 (MQ=255)                                                                                                                                                             tttGATGTAGCCCGGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGAGGTATCGCATTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTTCGCCTTATCGGCTGCATCCGCTTCGCCCACGTTAACTCGTTTAGCTGGCGGCAGCGTAGTAATCTTcgagctcgtgtcagccagact                                                                                     >  2:435507/1‑230 (MQ=255)                                                                                                                                                                 aagTAGCGCATCGCATCAAGTAATGAGGAAGTTGAGTCACACGGCACTTCCTAGAATACGCTATGCGGTGCGGTATCGACTATCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAACCCTTCGCCTTCTCCTTCTTATTGGCTGATTCAGCCTTATCGGCTGCATCAGCTTCCCCCACGTAAACGCTCTTTGCTGGCGGCAGCGGAGTAATCTTCACGTCGCTGTACACCAGGCGAGTct                                                                                >  2:392254/3‑250 (MQ=255)                                                                                                                                                                         cAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTG‑CCATc                                                                        >  1:522946/1‑250 (MQ=255)                                                                                                                                                                         cAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTAGGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTG‑CCATCa                                                                       <  2:522946/251‑1 (MQ=255)                                                                                                                                                                          aGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTGCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTG‑CCATCAg                                                                      <  1:29031/251‑1 (MQ=255)                                                                                                                                                                            cgcATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTG‑CCATCAGCa                                                                    <  1:133211/251‑1 (MQ=255)                                                                                                                                                                               aTCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTCTAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTACAGGCGAGTCGGG‑CCATCAGAATcc                                                                 >  2:357619/1‑250 (MQ=255)                                                                                                                                                                                tCCAGTAATGAGGAAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCCGCGTAATAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTG‑CCATCCGCATcgc                                                                >  1:399239/1‑251 (MQ=255)                                                                                                                                                                                  cAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGCATGCGCTATGCGGTGGGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCTGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCCTCGCCACCGTAAGCGCGGTTAGCTGGCGGCAGCGTAGTACTCTTAACGTAGCTGTACTACAGGCGAGTCGTG‑CCATCAGCATCGCgg                                                              >  1:283529/1‑251 (MQ=255)                                                                                                                                                                                  cAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTCGTAATCTTCACTCCCCTGTACTCCAGGCGAGTCGTG‑CCCTCAGCATCGCgg                                                              >  2:153836/1‑251 (MQ=255)                                                                                                                                                                                      aaTGAGGAAGTTGCGTCATAAGGCACTTCAAAGAATGCGTTATTCGGTTCGGTATCGACTTTCGGGTTATAGCGCCC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCGTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTG‑CCATCAGCATCGCGGAAg                                                           <  2:140210/250‑1 (MQ=255)                                                                                                                                                                                        tGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCTGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTG‑CCATCAGCATCGCGGAAGGCg                                                        >  1:279538/1‑251 (MQ=255)                                                                                                                                                                                        tGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGGGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGGCGTG‑CCATCAg                                                                      >  2:435758/1‑237 (MQ=255)                                                                                                                                                                                        tGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCACTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTG‑CCATCAg                                                                      <  1:435758/237‑1 (MQ=255)                                                                                                                                                                                            gtatttGCGTCATAAGGCCCTTCATAGAATGCGCTATGCGGTGGGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCCGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGGCGCTGTACTCCAGGCGAGTCGTG‑CCATCAGCATCGCGGAAGGCGAggg                                                    <  2:156025/247‑1 (MQ=255)                                                                                                                                                                                               gTTGCGTCATAAGGCACTTCAGAGAATGAGCTATGCGGTGCGGTATCGACTTTAGGGTTATAGCTCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGATTCCCCACCCTACACGCGTTTAGATGCCGGCAGCTTAGTTATCATCACCTCTCTGTGCTCCACGCGCGTCGGG‑CCCTCATCCTCCAGGAAGGCGAggggct                                                 >  2:192973/1‑248 (MQ=255)                                                                                                                                                                                                    gTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTG‑CCATCAGCATCGCGGAAGGCGAGGGTGTGTTTg                                            >  1:25749/1‑251 (MQ=255)                                                                                                                                                                                                    gTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTG‑CCATCAGCATCGCGGAAGGCGAGGGTGTGTTTg                                            <  1:558914/251‑1 (MQ=255)                                                                                                                                                                                                    gTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTG‑CCATCAGCATCGCGGAAGGCGAGGGTGTGTTTg                                            >  1:60598/1‑251 (MQ=255)                                                                                                                                                                                                    gTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCACTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCACTGTACTCCAGGCGAGGCGTG‑CCATCAGCTTAGCGGAAggagggggagtttttg                                            >  2:394388/1‑237 (MQ=255)                                                                                                                                                                                                      cATAATGCACTCCAGAGAATGCGCTATGCGGTGCGGTATCGACGTTCTGGTTATAGCGCTC‑CACCTCAATTTTCAGTTTTTTCAGCTCATCCAGTCGCCTTCTCCTTCTTATTGGCGGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCGGGCGGCAGCGTAGTAATCTTCACGTCGCGGTACTCCTGGCGAGTCGTG‑CCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAg                                          <  2:360161/251‑1 (MQ=255)                                                                                                                                                                                                      cATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTG‑CCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAg                                          <  1:396768/251‑1 (MQ=255)                                                                                                                                                                                                        tAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTG‑CCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAgg                                         >  1:154015/1‑250 (MQ=255)                                                                                                                                                                                                          aGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTG‑CCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGa                                        >  1:388472/1‑249 (MQ=255)                                                                                                                                                                                                           tgCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCCCGTCGCTGTACTCCAGGCGAGTCGTG‑CCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGaggt                                     >  2:120021/2‑248 (MQ=255)                                                                                                                                                                                                                           cgcTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTG‑CCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGAAGTTGACGTCGTCACGCTc                     >  1:145600/1‑251 (MQ=255)                                                                                                                                                                                                                               aTGCGGAGCGGTATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAACAGCGTTTAGCTGGCGGCAGCGTAGTAATATTCACGTCGCTGTACTCCAGGCGAGTCGTG‑CCATCAGCATCGCGGGAGGCGAGGGGGTGGTTGGGGACATTGACGTCGTCACGCCCgg                   >  2:189998/1‑249 (MQ=255)                                                                                                                                                                                                                                      gcggtATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTG‑CCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCtt          <  1:120021/251‑1 (MQ=255)                                                                                                                                                                                                                                       cggtATCGACTTTCGGGTTATAGCGCCC‑CCTCCCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTG‑CCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTc         <  2:515875/251‑1 (MQ=255)                                                                                                                                                                                                                                       cggtATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTG‑CCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTc         >  1:351621/1‑251 (MQ=255)                                                                                                                                                                                                                                        ggtATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTG‑CCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCg        >  1:484849/1‑251 (MQ=255)                                                                                                                                                                                                                                        ggtATCGACTTTCGGGTTATAGCGCAC‑CACCACAATTTTCAGGCTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGCAACCGCGTTTAGCTGGCGGCAGCGGAGTAATCTTCACGCCGCTGTACTCCAGGCGAGTCGTG‑CCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGTGGAAGTTGACGTCGTAACGCTCGGtccgaccctcg        >  2:231352/1‑241 (MQ=255)                                                                                                                                                                                                                                         gtATCGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTG‑CCATCAGCATCGCGGAAGGCGAGGGGGTGTTTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGt       >  1:318860/1‑251 (MQ=255)                                                                                                                                                                                                                                           aTCGACTTTAGGGTTATAGCGCAC‑CACCTAACTTTTCAGGTTTTTCATCTCCGCAATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTCGCTGGCGGCAGCGTAGTATTCTTCACGTCGCTGTACTCCAGGCGAGTCGGGGCCTTC‑GCATCGCGGACGGCGCGGGTGTGTTTGAGGAGGTTGACGTCGCCCCGCTAGGTGCAAcactcgtac     >  2:70590/1‑243 (MQ=255)                                                                                                                                                                                                                                             cGACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTG‑CCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCa    <  1:118596/250‑1 (MQ=255)                                                                                                                                                                                                                                              gACTTTCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCGTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTG‑CCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGa  <  1:258085/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                        GAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCAC‑CACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTG‑CCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGA  >  NC_000913/4380227‑4380714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑