Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,373,421	C→T	A272T (GCG→ACG)	yjeJ ←	protein YjeJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,373,421	0	C	T	98.5%	200.8 / NA	65	A272T (GCG→ACG)	yjeJ	protein YjeJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (28/36);  minor base G (0/1);  total (28/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

GACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGCGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGCCAGATAATGGTCGAACAAGTT  >  NC_000913/4373182‑4373667                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       gACAAACGTATAATGGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTACGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCAt                                                                                                                                                                                                                                              <  1:109232/250‑1 (MQ=255)        ttattaTTGCCTGATGCTCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGCATGCTTGAGAATTTGTCGGCCGGCTAAGCCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGAAGTTATGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATccgg                                                                                                                                                                                                                                      >  2:175551/6‑249 (MQ=255)         tataATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTgg                                                                                                                                                                                                                                     >  1:70928/1‑251 (MQ=255)           taATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGt                                                                                                                                                                                                                                   >  1:128244/1‑251 (MQ=255)               tGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAg                                                                                                                                                                                                                                <  1:475314/250‑1 (MQ=255)                gccggATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCg                                                                                                                                                                                                                              <  2:298873/247‑1 (MQ=255)                gCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGc                                                                                                                                                                                                                               <  1:255740/250‑1 (MQ=255)                 ccTGATGCGCTGCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCgg                                                                                                                                                                                                                             <  1:21422/251‑1 (MQ=255)                     aTGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTgg                                                                                                                                                                                                                         >  1:451949/1‑251 (MQ=255)                          cttcgcttATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGCTGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGAGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGtt                                                                                                                                                                                                                    >  2:502512/1‑251 (MQ=255)                          cttcgcttATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGACCGGATAAGGCGTTTACGCAGCATCCGGCATGACCAACGATCACGTTATCAGTAATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGtt                                                                                                                                                                                                                    >  1:526680/1‑251 (MQ=255)                              gcttATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTc                                                                                                                                                                                                                >  1:135306/1‑251 (MQ=255)                                  aTCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTcgcc                                                                                                                                                                                                             <  1:218504/250‑1 (MQ=255)                                        ccTACATAAGGCAGTGCAAGTGATTGAATGATTGAGAATTTGCAGGCCGGATAAGGCGTTTAAGCCGCATCAGGCATGAACAACGATAACGCTATCAGTCATTTCGGGCTGGATGGCCCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGATTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGAACGAATGTATCCTAGGTTAGCGGTTGGGCGTTATTCGCCGCTACgg                                                                                                                                                                                                      >  2:296450/1‑251 (MQ=255)                                          tACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCAGTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTAAGGTg                                                                                                                                                                                                    >  2:432037/1‑251 (MQ=255)                                                     ttGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGACCATAGAc                                                                                                                                                                                         >  1:63069/1‑251 (MQ=255)                                                        cAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTg                                                                                                                                                                                      >  1:519105/1‑251 (MQ=255)                                                         aaCTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGa                                                                                                                                                                                     <  1:354371/251‑1 (MQ=255)                                                             gATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACAt                                                                                                                                                                                  <  1:249440/250‑1 (MQ=255)                                                              attgaacgagtGAGAATTTGAAGACAGGATAAGGCGTTTACGAAGAATCCGGCCTGAACAACGATCACGTTATCAGTCAGTTAAGGATGAATGGCTCCAGCCTCGCATTACAAAAAATGAGATTATTTCGACTGTGGTGCAGTTTTGCTGGTGGCTTGAACACGTAAGAG‑ATGCAGCGTACGTACGAGATGATCCTGGGTTAGCGGGTGGACGTTATTCGCCGCTACGGTGCGAACATAGACCtacaatgg                                                                                                                                                                                >  2:83999/11‑244 (MQ=255)                                                                           gaATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGt                                                                                                                                                                   <  1:353270/251‑1 (MQ=255)                                                                            aaTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTa                                                                                                                                                                                                                                 <  1:131010/188‑1 (MQ=255)                                                                            aaTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTa                                                                                                                                                                                                                                 >  2:131010/1‑188 (MQ=255)                                                                            aaTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGtt                                                                                                                                                                  >  1:359139/1‑251 (MQ=255)                                                                               ttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGtttt                                                                                                                                                                <  1:426003/250‑1 (MQ=255)                                                                                        ggATAATGCGTTTACGCCGTATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTTCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGTGGGTGGGCGTTATTCGCCGCTACGGTGCGAGCATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTg                                                                                                                                                      <  2:233142/251‑1 (MQ=255)                                                                                          aTAAGGCGTTTGCGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCCTTTCAGGCTGGATGTCTCCAGCCTCGCATTAGCAAAGATGAGATTATTCCGCCTGTGGGGCAGTTTGGCTGTTGGATTGAACACGTAAGGG‑ATGCTGCGGGCGTAAGCATTGATCCTGGGTTATCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTgg                                                                                                                                                     <  2:369773/250‑1 (MQ=255)                                                                                               gCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATACTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGa                                                                                                                                                >  2:87939/1‑250 (MQ=255)                                                                                                      cgccgcATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACAGGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGc                                                                                                                                        <  1:495889/251‑1 (MQ=255)                                                                                                       gccgtATCCGGCATGAACAACGATCACGTTATCAGTCATTTCAGGCTGGATGGTCCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTAGTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCa                                                                                                                                       <  2:69030/251‑1 (MQ=255)                                                                                                           cATCCGGCTTGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATGTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCg                                                                                                                                   <  2:363836/251‑1 (MQ=255)                                                                                                            aTCCGGCATGACCAACGTTCACGTTATCAGTCAGTTCAGGCTGGATGGTGCCAGCGTCGCAGTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTGAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCgg                                                                                                                                  <  2:58831/251‑1 (MQ=255)                                                                                                                  cctgacccacgaataCGTTATCAGGCACTTCAGGCTGGATGGCTCCAGGCTCGCATTAGCAAAGCTGAGATTATTTCGCTCGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGGTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTg                                                                                                                            <  2:377972/237‑1 (MQ=255)                                                                                                                      cccAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCg                                                                                                                        <  2:359139/250‑1 (MQ=255)                                                                                                                        caacgaataCGTTATAAGTCATTTAAGACTGGATGGCTCTAGCCACGCATTAGCAAAAATGAGCTTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGCACCCGTAAGAG‑ATGCAGCGTGCGGAAGATTTGATCCTGGGTTAGCGGTTGGGCGTTAGTCGCCGCTACGGCGCGACCATAGCCCTGACATGGCACCCCCGCGCGTTTGTTCCGTCGTGGTATGAAATCAAGATTTCGGCgggcagtcgat                                                                                                                      >  2:353631/9‑241 (MQ=255)                                                                                                                            gATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCt                                                                                                                   >  1:95918/1‑250 (MQ=255)                                                                                                                               cACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTGTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTAttt                                                                                                               <  1:348728/251‑1 (MQ=255)                                                                                                                                        aGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATGATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCg                                                                                                      <  1:314986/251‑1 (MQ=255)                                                                                                                                           aaTTTCAGGCGGGATGGCTAAAGCCTCGCATTAACAAAGATGAGATTATTTAGACTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAATTG‑ATGCAGCGTGCGTAAGAATTGAGCCTGGGTTAGCGGTTGGGCTTTATTCGCCGCTACGGTGCGAACATAGACCTGACAGGGCACACCCGCGAGTTTTTTTAGTCGTGGGCTGAAATCAAGCATACGGCGAGTGATCGCTTCTAGTTCTTTACCCGGCg                                                                                                   <  2:328812/250‑1 (MQ=255)                                                                                                                                               gcAGGCGGGATGGCTCCAGTCTAGAATTAGCAAAGAGGAGATTATTTCGCCTGTGGTGCAGTTTGGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACAGAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTg                                                                                                <  2:135306/249‑1 (MQ=255)                                                                                                                                                 gggcgggATGGCTCCAGCCTCGCTTTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCGGGTGGATGGAACACGTAAGTG‑AGGCAGCTTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCtt                                                                                             <  2:519105/246‑1 (MQ=255)                                                                                                                                                  ggCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTc                                                                                            <  1:323129/251‑1 (MQ=255)                                                                                                                                                              ccAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTc                                                                                  <  1:483950/249‑1 (MQ=255)                                                                                                                                                                aGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTcacca                                                                              <  1:502512/251‑1 (MQ=255)                                                                                                                                                                aGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTcacca                                                                              <  1:246610/251‑1 (MQ=255)                                                                                                                                                                 gCCTCGCATTAGCAAAGATGAGATTATTGCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTcaccac                                                                             <  1:441400/251‑1 (MQ=255)                                                                                                                                                                     cGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGc                                                                                                                                                                                                                               >  2:444954/1‑101 (MQ=255)                                                                                                                                                                     cGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGc                                                                                                                                                                                                                               <  1:444954/101‑1 (MQ=255)                                                                                                                                                                       cATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGcgcc                                                                       <  1:175551/251‑1 (MQ=255)                                                                                                                                                                       cATTAGCAAAGATGAGATTATTGCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGACCATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGcgcc                                                                       <  2:238884/251‑1 (MQ=255)                                                                                                                                                                               aaGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAAtt                                                               >  2:475483/1‑251 (MQ=255)                                                                                                                                                                                 gattagaTTATTGCGCCTGTGGTGCAGTGTTGCTGTTGGTTTGAACACGTAA‑TGTATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTAGTCGCCGCTACGGCGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGTGCTGAAATCAAGCATGCGGCGAGTGATCGCTTCTATTTCTTTTCCCGGCGTTGCTTCACGCGTTTTCGCCACTGCGCCGCTAAATTGt                                                             <  1:348412/247‑1 (MQ=255)                                                                                                                                                                                     agaTTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTACATTGTTCtt                                                         >  2:156234/1‑251 (MQ=255)                                                                                                                                                                                       attagtTCGCCTGTGGTGCAGTGTTGCTGGTGGATGGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATGCTGGGGTAGCGGTTGGGCGTTATTCGCCGCTACGGTTCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACTCGTGTTCTCCACTGCGCCGCTAAATTGTTCTTTg                                                       <  2:63069/251‑1 (MQ=255)                                                                                                                                                                                             tCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACtt                                                 >  1:497297/1‑251 (MQ=255)                                                                                                                                                                                              cGCCTGTGGTGCAGTTATGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGGTCCTGGGTTAGCGGTTGGGAGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCAAACCCGCGAGTTTTTTAAGTAGTGGGCTGAAATAAAGCATTAGGCGGGTGATCGCTTCTATTTCTTTACCCGGCGATACTTCCCGCGTTATCACCACTGCGCCGCTAAATTGTTTTTTGCCCCttt                                                >  2:453537/1‑251 (MQ=255)                                                                                                                                                                                               gCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTc                                               >  1:498183/1‑251 (MQ=255)                                                                                                                                                                                                   gtggtgCGGTTTTGCTGGTGGATTGAACACGTAAGTG‑CGGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGCAATCAAGCATTCGGCGAGTGATCGCTTCTTTTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAATTTGTTCTTTGCCACTTTCATCt                                           >  2:88971/1‑251 (MQ=255)                                                                                                                                                                                                                gcttgggtcttGAACACGGAAGTG‑ATGTAGCGTGCGTAAGCATTGAGCCTGGTTTCGTGGTTGGGCGTTATGCGCCGATTCGGTTCGTAAATAGACCTGACAGGGCACGCACGCGATTTTTTGCTGTTGTGGGCTGAAATCAATCATTCTGCGATTTATCGCGTCTAGTTCTTTTCCCGGCGTTGCTTTACTCGTTTTCACCACTGCGCCGCCAAATTGTGCTTTGCCACTTTCATCTTTAAAGCGGTaaa                              <  2:48823/242‑1 (MQ=255)                                                                                                                                                                                                                  tggtggATTGAACACGTAAGTG‑ATGCAGCGTGCGTAAGCATTGTTCCGGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACACGGCACCCCCGCGAGCTTTTTCAGTCGTGGGCTGAAATCAATGATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAACCGGTAAAGc                            >  2:119627/1‑251 (MQ=255)                                                                                                                                                                                                                              cacGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGCCAGATa                 >  1:198584/1‑250 (MQ=255)                                                                                                                                                                                                                               acGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTCTTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCCCCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGCCAGATAAt               >  2:259602/1‑251 (MQ=255)                                                                                                                                                                                                                               acGTAAGTG‑ATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGCCAGATAAt               <  1:87939/251‑1 (MQ=255)                                                                                                                                                                                                                                         aTGCAGCGTGCGTAAGCATTGATACTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGCCATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGCGTGATCGCTTCTATTTCTTTACCCGGCGATGCTTCACGCGTTTTCCCCACTGAGCCGATAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACAGACAGATAATGGTCGAAc       >  2:44202/1‑250 (MQ=255)                                                                                                                                                                                                                                             aGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGCCAGATAATGGTCGAACAAGtt  <  1:281742/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       GACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTG‑ATGCAGCGCGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGCCAGATAATGGTCGAACAAGTT  >  NC_000913/4373182‑4373667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑