Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,364,798	C→T	G82D (GGC→GAC)	dsbD ←	thiol‑disulfide exchange protein DsbD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,364,798	0	C	T	100.0%	224.7 / NA	70	G82D (GGC→GAC)	dsbD	thiol‑disulfide exchange protein DsbD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/34);  total (36/34)

CGGTGGGCTGCTCTTGCTGCGGAACAGACACAGGCTGTGGCGCTGCGTTGTTGGCGACCACTTCGCTTAACGGAACGGTTTTGGTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGCCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTCCCGGCGCGTCGAATAATCCGGCAAAAACGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGC  >  NC_000913/4364562‑4365039                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                  gggtggtCTGCCCTTGCTGTGGAACAGACACAGGCTGTGGCGCTGCGTTGTTGGCGACCACTTCGCTTAACGGAACGGTTTTGGTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGc                                                                                                                                                                                                                                     <  2:401389/250‑1 (MQ=255)      tgctgctCTTGCTGCGGAACAGACACAGGCTGTGGCGCTGCGTTGTTGGCGACCACTTCGATTAACGGAACGGTTTTGGTTTCTGGCGGATAACAGACACCGGCATCAGCAAAGCCCTGGTAGGGGCCGGTTAACGTCGCTCCCGCACTCGCCTGGCTGATGGTGACGGGAAGCGTCAGCCGATCGCGGGACATCCCGCTTTTGCCGTAACACTCATCTTCCTGCCAGACGTCTTGAGGCAGCTGCACGTc                                                                                                                                                                                                                                >  2:423775/2‑251 (MQ=255)      ggCTGCTCTTGCTGCGGAACAGACACAGGCTGTGGCGCTGCGTTGTTGGCGACCACTTCGCTTAACGGAACGGTTTTGGTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGTTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTc                                                                                                                                                                                                                                >  1:568801/1‑251 (MQ=255)       gcggctCTTGCTGCGGAAGAGACACAGGCTTTGGTGCTTAGTTGTTGGCGACCACGTCGCTTAATGGAACGGTGTTGGTTTCGTGCGGATGACAGAAACCGGCATCAGCACAGCCCTGGTAGGGGACAGTTAACGTCGTTCCCGCACGCGCCTGGTTGATTGGGACGGGATGCGGAAGCCGATCGCGGTAGTTCGCGCTTTTTCCGTATAACTCAGCTTCATGCCAGATGTCTTGCGGCAGCGTCACGTCg                                                                                                                                                                                                                               <  2:57908/248‑1 (MQ=255)                      gAACAGACACAGGCTGTGGCGCTGCGTTGTTGGCGACCACTTCGCTTAACGGAACGGTTTTGGTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCgtgt                                                                                                                                                                                                                <  1:557208/251‑1 (MQ=255)                        aCAGACACAGGCTGTGGCGCTGCGTTGTTGGCGACCACTTCGCTTAACGGAACGGTTTTGGTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTc                                                                                                                                                                                                              >  1:265541/1‑251 (MQ=255)                          aGACACAGGCTGTGGCGCTGCGTTGTTGGCGACCACTTCGCTTAACGGAACGGTTTTGGTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCg                                                                                                                                                                                                            <  1:99625/251‑1 (MQ=255)                            acacAGGCTGTGGCGCTGCGTTGTTGGCGACCACTTCGCTTAACGGAACGGTTTTGGTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCACTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGATGATGGTTACGGGAAGCGTCAGCCGATCGCGGTAAAACTCGCTTTTTCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCGGCCCGTCGGCACTTTTCGCGTGTTCCg                                                                                                                                                                                                            >  2:170768/1‑249 (MQ=255)                                    cgtgGCGCTGCGTTGTTGGCGACCACTTCGCTTAACGGAACGGTTTTGGTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCg                                                                                                                                                                                                  <  2:208039/250‑1 (MQ=255)                                         cgcTGCGTTGTTGGCGACCACTTCGCTTAACGGAACGGTTTTGGTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCt                                                                                                                                                                                             <  1:296119/251‑1 (MQ=255)                                            tGCGTTGTTGGCGACCACTTCGCTTAACGGAACGGTTTTGGTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCCATTTTCGCGTGTTCCGGCGTAATGAGGTTCTGtt                                                                                                                                                                                          >  2:329118/1‑251 (MQ=255)                                               gttgttGGCGACCTCTTCGCTTAACGGAACGGTTTTGGTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTATCGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGGCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTAc                                                                                                                                                                                       <  2:240964/251‑1 (MQ=255)                                               gttgttAGCGACCACTTCGCGTATGGGAACGGTTTTGGTTTCTGGCGGATAACGTAAACCGGCATCACCTCAGCCCTGGTAGGTGACAGTTAACGCCGCTCCCCGACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCGCGCTTTTGCCGTAAAACTCATCTTCATGCCCGACGTCTTGCGGCATCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATTCGGATCTTTTTAc                                                                                                                                                                                       <  2:136563/251‑1 (MQ=255)                                                 tgttgGCGACCACGTCGCTTAACGGAACGGTTTTGGTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACg                                                                                                                                                                                      <  1:206979/250‑1 (MQ=255)                                                     ggCGCCCACGTCGCTGAACTGAACGGTTTTGGTTTCTGGCGGATAAAATAAACCGGAAGCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTaga                                                                                                                                                                                 <  2:6935/251‑1 (MQ=255)                                                               tCGCTTAACGGAACGGTTTTGGTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAAc                                                                                                                                                                        <  2:288860/250‑1 (MQ=255)                                                                cGCTTAACGGAACGGTTTTGGTTTCTGGCGGATAACAGAAACCGGCATCAGCCCAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCg                                                                                                                                                                      <  1:117236/251‑1 (MQ=255)                                                                cGCTTAACGGAACGGTTTTGGTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCg                                                                                                                                                                      >  1:343477/1‑251 (MQ=255)                                                                     aacggaacggTTTTGGTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGGAGTAACCGTCttt                                                                                                                                                                 >  1:1/1‑251 (MQ=255)                                                                       cggaacggTTTTGGTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGAGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGTGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAAACGTCTTTgg                                                                                                                                                               >  1:499784/1‑250 (MQ=255)                                                                          aactgTTTTTGTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGTTAGGTGACAGGTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGGCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCt                                                                                                                                                            <  2:278868/247‑1 (MQ=255)                                                                           acggTTTTGGTTTCTGGCGGATAACAGAAACCGGCAGCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTg                                                                                                                                                           <  1:533808/251‑1 (MQ=255)                                                                              ggtttGGTTTCTGGCGGTTAAAAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCa                                                                                                                                                        <  2:265438/249‑1 (MQ=255)                                                                                   ggTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTc                                                                                                                                                    <  1:55827/250‑1 (MQ=255)                                                                                    gTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAg                                                                                                                                                  <  1:26165/251‑1 (MQ=255)                                                                                         tGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTa                                                                                                                                              >  1:298412/1‑250 (MQ=255)                                                                                          ggCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTa                                                                                                                                              <  1:69481/249‑1 (MQ=255)                                                                                                 aaCAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGt                                                                                                                                     >  1:446642/1‑251 (MQ=255)                                                                                                      aaaCCGGCATCAGCACAGCCCTGGTAGGTGACGGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGtt                                                                                                                                >  1:22942/1‑251 (MQ=255)                                                                                                       aaCCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCCTCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCCATTTTCGCGTGTTCCGGGGGCATGCGGATCTGTTTACGGTAGAGGGAGTACCAGTCATTGATCGGCCCGGTCCGATTAAGGTCATGTTGGttt                                                                                                                               >  2:404344/1‑251 (MQ=255)                                                                                                                cAGCACAGCCCAGGTAGGTGACAGTTCACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTTGCGTGTTCCGGCGTACTGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGaaa                                                                                                                       >  2:75363/1‑250 (MQ=255)                                                                                                                   cacaGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCCGGTCAGATTAAGGTAATGTTGGTTTTGCTGAAAATc                                                                                                                    >  2:436971/1‑250 (MQ=255)                                                                                                                   cacaGCCCGGGTAGGTGCAAGTTAACGTCGCTCCCGCACTCGCCTGGTGGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCa                                                                                                                   <  2:9584/251‑1 (MQ=255)                                                                                                                     caGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCCCGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCCGGTCCGATTAAAGGCATGTTGGTTTTGCTGAAAATCaa                                                                                                                  >  2:385149/1‑250 (MQ=255)                                                                                                                     caGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAgg                                                                                                                                                                               <  2:217701/189‑1 (MQ=255)                                                                                                                     caGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAgg                                                                                                                                                                               >  1:217701/1‑189 (MQ=255)                                                                                                                       gCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCCCTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAg                                                                                                               <  2:560044/251‑1 (MQ=255)                                                                                                                         ccTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCCATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTGGTAACCGTCTTTGATTTGCCAGGTCAGATTAAGGTCCTGGTGGGTTTGCTGAAAATCAAAAGc                                                                                                              >  2:133863/1‑250 (MQ=255)                                                                                                                             gTAGGTGACAGTTAACGTAGCTCCCGAAAGTGCCTGGTGGAGGGGGACGGGAAGCGTCAGCCGTTCGCGGTAAATCTCGCTGTTGACGTAAAACGTAGCTGCATGGCAGACGTCGTGCGGCAGCTGCACGTCGGCAAGTTGCGCGTGTACCGGGGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATATGCCAGGGCAGAGTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAgg                                                                                                         <  2:415979/251‑1 (MQ=255)                                                                                                                               aGGTGACAGTTAACGTCGCTCCCGCCCTCGCCTGGTTGATGGTGACGGGAAGCGTCAGACGCTCGCGGTACATCTCGCTTTTGCCGTAAAACTCCTCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGGTTAAGGTCATGTTGGTTTTGCTGACAATCAAAAGCAAAggat                                                                                                       >  2:76557/1‑249 (MQ=255)                                                                                                                                     cAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATcc                                                                                                 >  1:258098/1‑251 (MQ=255)                                                                                                                                        ttAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCCGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCcgcg                                                                                              >  2:246382/1‑251 (MQ=255)                                                                                                                                         tAACGTCGCTCCCGCACTCGACTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCAGAAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGGTGCACGTAGGCACTTTTCGCGTGTTCCGGCGAACTGCGGGTCTGTTTACGGGAGAGGTAATAACCGTCTTTGATCTGCCAGGACAGATTAAGGTAATGTTGGGTTTGCTGAAAATTAAAACCAAAGGCTTGATCcgggg                                                                                             >  2:435956/1‑248 (MQ=255)                                                                                                                                          agcgtcgtTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCAGGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCggg                                                                                            <  2:236282/249‑1 (MQ=255)                                                                                                                                             gTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAACACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCACTTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGGAGAGGTAGAAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTAGTTTTGCTGAAAATCAAAAGCAAAAGCTTGATCCGCGGGGAc                                                                                         >  2:332320/1‑251 (MQ=255)                                                                                                                                             gTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGAc                                                                                         >  1:252089/1‑251 (MQ=255)                                                                                                                                                  ccccGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGACAAAtt                                                                                    <  1:75363/250‑1 (MQ=255)                                                                                                                                                             ccTGGTTGATGGTGACGGGACGCGTCAGCCGATCGCGGTAAATCGCGCTTTTGCCGTAAAACTACTCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTACTTCGGATCTTTTTACGGTAGAGGGAGTACCCGTCTTTGATCTACCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGTAAATCAAAAGCAAAGGCTTGCTACGCGGTGACAAATTTTGAActaccc                                                                         >  2:97378/1‑246 (MQ=255)                                                                                                                                                              cTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTCCCg                                                                        >  1:294824/1‑251 (MQ=255)                                                                                                                                                                   tGATGGTGACGGGAAGCGTCAGCCGCTCGCGGTAAATCTCGCTTTTGCCGTAACACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTAGAGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGGTCTGCCAGGTCAGATTAAGGTACTGTTGGGTTTGCTGAAAATCCAAAGCCACAGCTTGATCCGCTGGGACAAATTGAGTACGTCCCggaggg                                                                   >  2:335382/1‑247 (MQ=255)                                                                                                                                                                            cGGGAAGCGTCTGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGGTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTCCCGGCGCGTCGaataat                                                          <  1:335382/251‑1 (MQ=255)                                                                                                                                                                              ggAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTCCCGGCGCGTCGAATAATcc                                                        <  1:182761/251‑1 (MQ=255)                                                                                                                                                                               gAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCCATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTCCCGGCGCGTCGAATAATCCg                                                       >  1:430781/1‑251 (MQ=255)                                                                                                                                                                                  gCGTAAGCCGATCGAGTTAAATCTCGATTTGGACGTCAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCGGCACGTCGGCAAGTTGCGCGTGTTCCGGCGTAAGGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGGCAGGTTGGTTTTGCTGAAAAGCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTCCCGGCGCGTCGCATAATCCGGCa                                                    <  2:1/251‑1 (MQ=255)                                                                                                                                                                                     tCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTCCCGGCGCGTCGAATAATCCGGCaaa                                                  >  1:564469/1‑250 (MQ=255)                                                                                                                                                                                        gCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGACGGCAATTTTCGCGTGTTCCGGCATAATGCGGATCTGTTTACGGTAGAGGTAGTAACGGTCTTTGATCTGCCAGGTCAGGTTAAGGTCATGTTGGATTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTCCCGACGCGTCGAAAAATCCTGCCAAAACg                                              >  2:450914/1‑251 (MQ=255)                                                                                                                                                                                          cGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGCCGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGCCAAATTGTGAACGTCCCGGCGCGTCGAATAATCCGGCAAAAACgg                                             >  2:541064/1‑250 (MQ=255)                                                                                                                                                                                                cgGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTCCCGGCGCGTCGAATAATCCGGCAAAACCGGAAGtgct                                      >  1:211250/1‑251 (MQ=255)                                                                                                                                                                                                  gTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCGAGACGTCTTGCTGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGc                                                                                                              <  1:78502/177‑1 (MQ=255)                                                                                                                                                                                                     aaTCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTCCCGGCGCGTCGAATAATCCGGCAAAAACGGAAGTGCTGCaa                                  >  1:235274/1‑250 (MQ=255)                                                                                                                                                                                                          cGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTGCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTCCCGGCGCGTCGAATAATCCGGCAAAAACGGAAGTGCTGCAAAg                                <  2:252089/247‑1 (MQ=255)                                                                                                                                                                                                          cGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGGCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTCCCGGCGCGTCGAATAATCCGGCAAAAACGGAAGTGCTGCAAAGTAGc                            <  1:86065/251‑1 (MQ=255)                                                                                                                                                                                                             ttttGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTCCCGGCGCGTCGAATAATCCGGCAAAAACGGAAGTGCTGCAAAGTAGCAgg                         >  1:105929/1‑251 (MQ=255)                                                                                                                                                                                                              tttGCCGTAAAACTCATCTTCATGCCCGACGTCTTGCGGCAGCCGCACGTCGGCAATTTTCTCGTGTTCCGGCGTAATGCGGATTGGTTTACGGTAGAGGTAGTAACCGTCTTTGAGCTGCCAGGGCAGATTAAGGTCATGTTGTTTGTGCTGAAAATCAAAAGCAAAGGCTTGAGCCGCGGGGACAAATTGTGAACGTCCTGGCGCGTCGAATAATCCGGCAAAAACGGAAGTGCTGCAAAGTAGCAg                          <  2:330340/249‑1 (MQ=255)                                                                                                                                                                                                                tGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTCCCGGCGCGTCGAATAATCCGGCAAAAACGGAAGTGCTGCAAAGTAGCAGGAt                       <  1:446491/250‑1 (MQ=255)                                                                                                                                                                                                                   cGTAAAACTCATCTTCATGCCAGACGTCTTTCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTTAGGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTTGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTACCGGCGCGTCGAATAATCCGGCAAAAACGGAAGTGCTGCAAATTAGCAGGATCAGc                   <  2:298412/251‑1 (MQ=255)                                                                                                                                                                                                                   cGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTCCCGGCGCGTCGAATAATCCGGCAAAAACGGAAGTGCTGCAAAGTAGCAGGATCAGc                   >  1:167647/1‑251 (MQ=255)                                                                                                                                                                                                                               cTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGACAATCAAAAGCCAAGACTTGATCCGCGGGGACAAATTGTGAACGTCCCGGCGCGTCGAAAAATCCGGCAAACACGGAAGTGCTGCAAAGTATCAGGATCAGCGTAAAGATGCGt       >  2:342242/1‑251 (MQ=255)                                                                                                                                                                                                                                   aTGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTCCCGGCGCGTCGAATAATCCGGCAAAAACGGAAGAGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTg     >  1:459566/1‑249 (MQ=255)                                                                                                                                                                                                                                     gCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTCCCGGCGCGTCGAATAATCCGGCAAAAACGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGc  <  1:436520/250‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                  CGGTGGGCTGCTCTTGCTGCGGAACAGACACAGGCTGTGGCGCTGCGTTGTTGGCGACCACTTCGCTTAACGGAACGGTTTTGGTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGCCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTCCCGGCGCGTCGAATAATCCGGCAAAAACGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGC  >  NC_000913/4364562‑4365039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑