Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,349,259	C→T	intergenic (‑515/+56)	dcuB ← / ← dcuR	anaerobic C4‑dicarboxylate transporter DcuB/DNA‑binding transcriptional activator DcuR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,349,259	0	C	T	100.0%	136.3 / NA	42	intergenic (‑515/+56)	dcuB/dcuR	anaerobic C4‑dicarboxylate transporter DcuB/DNA‑binding transcriptional activator DcuR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/14);  total (28/14)

GTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGCCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTG‑GACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACGGCAGGAAACA‑CGCGAAATGTTAACCTCG‑TTGGCTAGCTCGTCGGTTGAAAAT  >  NC_000913/4349027‑4349501                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      gtatATTGTTTGTTCTAAGTGCAGATGTCATTAGGTTGATTGATGAATCTGATCTACCATTTTGTGGGTAAAAATACACATAATGCGGGATACATAATAGTTAATTAACTTTTGTTAGCGGTTCGAAATTAAAAACGCCGTTCACCTGAAGAGATATGAATTTTTAGCGCTTATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGGTGAAGGATCTAATTTGTATCATGGGTATTTTGCATTa                                                                                                                                                                                                                                     <  2:400948/248‑1 (MQ=255)            gTTCTCAGGGCAGATGACATTAGGTTTATAGATTAATCTGCTCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGCCATAATAGTTAATTATCTATTGTTAGCGTTTTGAAATTAAAAACACCGTTCGCCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACCAGTTTTACTGATGAAGGATGAAACTTGTGTCAGGGGGATTTTGCTTTACgtatatattt                                                                                                                                                                                                                          >  2:61986/1‑242 (MQ=255)                       aGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTAttacttac                                                                                                                                                                                                                 >  1:178571/1‑249 (MQ=255)                             caatAGGTTTATAGACTAATATGATCGACCCATTTGTGTGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAAGTATTACTTCCAGATAAc                                                                                                                                                                                                          <  2:104428/247‑1 (MQ=255)                              aTTAGGTTTATAGAGTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACTCCGTTCACCTTAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACGGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACGTACAGATAACCa                                                                                                                                                                                                        <  2:344623/251‑1 (MQ=255)                                 aGGTTTATTGATTAATCTGATCTACCCATTTGTGGGTAAAAATACCCATAATGCGTGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTGAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCcgcg                                                                                                                                                                                                     <  2:170029/251‑1 (MQ=255)                                      tataGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTACTTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCg                                                                                                                                                                                                >  1:280869/1‑251 (MQ=255)                                                        aCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGt                                                                                                                                                                                <  1:555958/249‑1 (MQ=255)                                                                      aaaaaTACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCagtagtgagtagt                                                                                                                                                                >  1:22951/1‑251 (MQ=255)                                                                                     gCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTAttact                                                                                                                                                                                                                    <  1:303253/184‑1 (MQ=255)                                                                                     gCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTAttact                                                                                                                                                                                                                    >  2:303253/1‑184 (MQ=255)                                                                                                   aGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGTTGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACt                                                                                                                                   >  2:213055/1‑251 (MQ=255)                                                                                                      taattacCTTTTGTTAGCGTTTTGAAATTAAAAACCCCGTTCAACTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAGGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGTTCCGTTATTGGCAATATTGTTTTAGTAGAAAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTg‑gc                                                                                                                               >  2:353640/1‑250 (MQ=255)                                                                                                       aattaaCTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTG‑GAc                                                                                                                              >  1:271890/1‑251 (MQ=255)                                                                                                                  gTTAGCGGTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTTTCAGGGGTATTTTGCATTACGGTAATAATTAGTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTGTCAGTAGTGAGTAGTGTACTGCCTGAATACGGTAACGGTAAACTG‑GACGCCCCGTGACg                                                                                                                   <  1:571653/251‑1 (MQ=255)                                                                                                                    tAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTG‑GACGCCCCGTGACGcc                                                                                                                 >  1:211443/1‑251 (MQ=255)                                                                                                                    tAGCGCTTTGAAATTACCAACACCGTTCACCTGAAGCGATATTAATTTTTAGCGTTGATGGAGGGCTAATTATATTTGATCTGGCACAAGTTTTACTGATGACGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCAGTTATTGACAATATTGTTTCAGTAGTACGTAGTGTTCTGCCTGATTACGGTCACGGTAAA‑TGTGACGCCTCGTAACGcc                                                                                                                 >  2:274568/1‑251 (MQ=255)                                                                                                                       cGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTG‑GACGCCCCGTGACGCCAt                                                                                                               >  1:498502/1‑250 (MQ=255)                                                                                                                         ttttGAAATTAAAAACACCGTTCACATGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGAGTCAGGGGTATTTTGCATTACGGTAATCATTATTACTTACAGATAACCAGCGATACGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGCTTACGGGAACGGTAAACTG‑GACGCCCCGTGACGCaataat                                                                                                            >  2:440913/1‑251 (MQ=255)                                                                                                                             gAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTG‑GACGCCCCGTGACGCCATAATGGAt                                                                                                        >  1:29325/1‑251 (MQ=255)                                                                                                                                     aaaCACCGTTCACCTGAAGATATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGGTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGTCTGAATACGGTAACGGTAAACTG‑GACGACCCGTGACGCCATAATGGATACTGGTGa                                                                                                <  2:22951/251‑1 (MQ=255)                                                                                                                                                      agagATATTAATTTTTAGCGATTATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGAGAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATAcggtaacggtaa                                                                                                                                      <  1:527085/197‑1 (MQ=255)                                                                                                                                                      agagATATTAATTTTTAACGATGATGGAGGGCTAATTATCTCTGATATGGCACAAGTTTAACTGATGAAAGATGTAAATTGTGTCTGGGGTCTTTTGCATTCCGGTAATAATTATTACTTACAGATAACCAGCGATACGATATTGGCCATATTGTTTCAGTCGTGAGTAGTGTTCTGCCTGACTAcggtaacggtaa                                                                                                                                      >  2:527085/1‑197 (MQ=255)                                                                                                                                                       gagaTATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTG‑GACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTg                                                                               >  1:366937/1‑250 (MQ=255)                                                                                                                                                         gagatTAAGTTTTAGCGATGATGTAGGTATAAGTTTATTGGATCTGGCTCAAGTTTTTATGAGTAAGGATGTAATTTGTGTTATGGGTAGTTTGCATGACGGTAATAATTATTACTTACAGATAACCAGCGATGCGTTATTTGCAATATTGTTTCAGTAGTCAGTAGTGTTCTGCCTGAATACGGTAACGGGAAACTG‑GACGTCTCGTGACGCCATAATGGATACTGTTGAACAAGATGTGGCAGTTgtcc                                                                            <  2:471416/248‑4 (MQ=255)                                                                                                                                                                aTTTTTAGCGCTGATGGAGTGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTATTAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGTATACGGTAACGGTAAACTG‑GACGCCCAGTGACGCCATAATGGATACAGGTGAACAAGATGTGGCAGTTGAccagcc                                                                        >  2:112056/1‑248 (MQ=255)                                                                                                                                                                  ttttAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCATGAATACGGTAACGGTAAACTG‑GACGCCCCGTGACGCCATAATGGATACTGGTAAACAAGATGTGGCAGTTGACCAGCAAGATg                                                                   >  2:431601/1‑251 (MQ=255)                                                                                                                                                                   tttAGCGATGTTGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTTTGTATGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGCTTACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTACCTGAATACGGAAACGGTAATCTG‑GACGCCCCGTGACGCCATATTGGATACGTGTGGACATGATGTGGCAGTTggacagacaga                                                                     >  2:442618/1‑238 (MQ=255)                                                                                                                                                                     tAGCGATGATGGAGGGATAATTACATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTG‑GACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAgg                                                                >  1:408336/1‑251 (MQ=255)                                                                                                                                                                        cGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTG‑GACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCCGCCAGATgggggat                                                             >  2:117250/1‑245 (MQ=255)                                                                                                                                                                                 aGGGATAATTATATATGATCTGGCACACGTTTTACTGATGAAGGAGGTAACTTGTGTCAGGGGGATTTTGCATTAAGGTAATAATTCTTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAATAGAGTTCTGACTGAATACGGTAACGGTACACTG‑AACGCCACGGGACGCCATAATGGATACTGGGGAACAAACTGTGGCAGTTTACCAGCCGGAtagagtaattacgca                                                      >  2:314984/1‑235 (MQ=255)                                                                                                                                                                                    gATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTG‑GACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACGGCAGGaa                                                 <  1:440913/251‑1 (MQ=255)                                                                                                                                                                                          tttatTTGATCTGGCATAAGTTTTACTGATGAAGGATGTAATTTGTGTCAGGGGTATTTTGAATTACGGTAATAATTATGACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTGCAGTAGTGAGCAGTGTTCTGCCTGAATACGGTAACGTTAAACTG‑GACGCCCCGTGACGCCATAATTGATACTGGTGAACAAGATTTTGCAGTTGACCAGCCAGATGAGGTATTGACGGCAGGAAAAA‑cg                                           <  2:564493/248‑1 (MQ=255)                                                                                                                                                                                                tGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCAGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGCATATGGTAACGGTAAACTT‑GACGCCCCGTGACGCCATAATGGATACTGGAGCACAAGATGTGGCAGTTGACCCACCCGTTGAGGTATTTACAGCAGGAAAAA‑CGCGAAATg                                    >  2:186954/1‑251 (MQ=255)                                                                                                                                                                                                          caAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTG‑GACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACGGCAGGAAACA‑CGCGAAATGTTAACCTCG‑t                         >  1:92892/1‑251 (MQ=255)                                                                                                                                                                                                             gTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTTTTTTGCATTACGGTAATAATTATTACTTTCAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTTAGGAGTGTTCTGCCTGAATACGGTAACGGTAAACTG‑GACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACGGCAGGAAACA‑CGCGAAATGTTAACCTCG‑TTgg                      <  1:535591/251‑1 (MQ=255)                                                                                                                                                                                                                 tACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGTTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTTTTTCAGTAGTGAGTAGTGtt                                                                                                                                                             <  2:346663/115‑1 (MQ=255)                                                                                                                                                                                                                 tACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTCTTGGCAATATTGTTTCAGTAGTGAGTAGTGtt                                                                                                                                                             >  1:346663/1‑115 (MQ=255)                                                                                                                                                                                                                  aCTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTG‑GACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACGGCAGGAAACA‑CGCGAAATGTTAACCTCG‑TTGGCTAGc                 >  1:307700/1‑251 (MQ=255)                                                                                                                                                                                                                         aaGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTG‑GACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCCGATGAGGTATTTTCGGCAGGAAACAACG‑GAAATGTTAACCCCGGTTGG‑TAGCTCGTCgg          >  2:419691/1‑251 (MQ=255)                                                                                                                                                                                                                             aTGTAACTTGTGTCATGGGTATTTTGCATTACGGTAATAATTATTACTTAAAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTG‑GACGCCCCGTGACGCCATAATGGATACTGGCGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACGGCAGGAAACA‑CGCGAAATGTTAACCTCG‑TTGGCTAGCTCGTCGGTTg       >  1:220317/1‑250 (MQ=255)                                                                                                                                                                                                                                 aaCTTGTGTCAGGGGTATTTTGCATGACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTG‑GACGCCCCATGACGCCGTAATGGCTACTGGTGAACAAGATGGGGCAGTTGACCAACCAGATGAGGGATTTACGGCATGAAACA‑AGCGGAATGGTAACCTCG‑TTGGCTAGCACGTCGGTTGGAAAt  >  1:397521/1‑251 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      GTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGCCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTG‑GACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACGGCAGGAAACA‑CGCGAAATGTTAACCTCG‑TTGGCTAGCTCGTCGGTTGAAAAT  >  NC_000913/4349027‑4349501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑