Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,332,310	C→T	G321G (GGG→GGA)	basS ←	sensor histidine kinase BasS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,332,310	0	C	T	97.3%	111.2 / NA	37	G321G (GGG→GGA)	basS	sensor histidine kinase BasS
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (34/2);  total (34/3)
Fisher's exact test for biased strand distribution p-value = 8.11e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATCTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGTGCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCA‑GACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCT  >  NC_000913/4332069‑4332554                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      aagctctGGCGAGCATACCCTCCGCCAGGTTAACGGAGGAGAGTGCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTGTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAATCCCA‑GAcc                                                                                                                                                                                                                                              <  2:25030/247‑1 (MQ=255)    ctctGACGCGAATACTCTCCTCCAGGTTAACGGAGGAGAGTGCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTACAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGTTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCTGAAGTCTCTTGCCGGTTTTGCAGGAAAACCTCCCCGTGATGCAACTGTGGAATGCGGCTGACAATACTTAATCCCA‑GACCAATc                                                                                                                                                                                                                                          >  2:474012/1‑251 (MQ=255)        gACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGTGCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAATCCCA‑GACCAATCCCg                                                                                                                                                                                                                                       <  1:292853/250‑1 (MQ=255)         aCGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGTGCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAATCCCA‑GACCAATCCCGcc                                                                                                                                                                                                                                     >  1:554692/1‑251 (MQ=255)                         ccAGGTTAACGGAGGAGAGTGCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAATCCCA‑GACCAATCCCGCCATAACGGCTGTCCAt                                                                                                                                                                                                                      >  1:422650/1‑250 (MQ=255)                          cAGGTTAACAGAGGAGAGTGCAATGAACAACCGAGTTTAAGAAAGTTTAACTACCGAGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTAACCAGGCCGGCGTGCCGGAAGTCTCTTGCCGGTTTTGCCGGAAAAACTGCACGTGGTGCAACTGTGTAATGCGGCTGACAATACTTACTCCCA‑GACCAATCCCGCCATAACGGCAGGCCATAc                                                                                                                                                                                                                    >  2:292737/1‑251 (MQ=255)                             gTTAACGGAGGAGAGTGCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTACTGCGGCTGACAATACTTAATCCCA‑GACCAATCCCGCCCTAACGGCTGCCCATACGt                                                                                                                                                                                                                  >  2:511377/1‑250 (MQ=255)                                    gaggagAGTGCAATGAAAAACCGTGTATATGACAGTTTAACTACCGTGTTCAGCGTGCTGGAGGTCAGCAGCTTTCTTTATATCTGGTTTGACACGTACTGCTCTTTCTTCAGCAGTACCCAGGCCCGCGTTGCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATTCGGCTGACAATACTTAATCCCA‑GACCAATCCCGCCATAACGGCTACCCATACgacccaacgc                                                                                                                                                                                                          >  2:369188/1‑242 (MQ=255)                                                aTGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAACAACTGCCCGTGATGCAACTGTGTAATGCGGATGCCAATACTTAATCCCA‑GACCAATCCCGCCATCACGGCTGTCCATACGTACAAACCCTTTACTCAAcac                                                                                                                                                                                              >  2:500115/1‑249 (MQ=255)                                                  gAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAATCCCA‑GACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTccc                                                                                                                                                                                            >  2:121594/1‑251 (MQ=255)                                                                       tttAACTACCGTGTTAAGCGCGCTGGTAGTCAGCAGACTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACACTAACCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTACCCCTGATGAAACTGTGTAATGCGGCTGACAATACTAAATCCAA‑GACGTCTCCCGCCATAACGGCTGTTCATACGTACGAACGCGTTACTCCAAGCCCCGCAGTTAATCtcaccgatac                                                                                                                                                                       >  2:32755/1‑244 (MQ=255)                                                                            cTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAATCCCA‑GACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGt                                                                                                                                                                  >  1:520117/1‑251 (MQ=255)                                                                             tACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGCTCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGACAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAATCCCA‑GACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCCTTTACTCTCACAAATACCTGGTc                                                                                                                                                                 >  2:64682/1‑251 (MQ=255)                                                                                          gTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAATCCCA‑GACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAAc                                                                                                                                                    >  1:145688/1‑251 (MQ=255)                                                                                          gTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAATCCCA‑GACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAAc                                                                                                                                                    >  1:512724/1‑251 (MQ=255)                                                                                                      agcagcTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAATCCCA‑GACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCACCTGCCATGACCGc                                                                                                                                        >  1:565356/1‑251 (MQ=255)                                                                                                        cagcTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCCGCCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGCCAATACTTAATCCCA‑GACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCCTCTTCAACTGCCATGACCGCTc                                                                                                                                      >  1:326954/1‑251 (MQ=255)                                                                                                                                cACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAATCCCA‑GACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATc                                                                                                               >  1:416910/1‑250 (MQ=255)                                                                                                                                  cGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAATCCCA‑TACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCAt                                                                                                             >  1:235615/1‑250 (MQ=255)                                                                                                                                   gTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAATCCCA‑GACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATa                                                                                                            >  1:420576/1‑250 (MQ=255)                                                                                                                                       tGATCTTTCTTCAGCAGAACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGAAGGAAAAACTGCCCGTGATGCAGATGTGTAATGCGGCTGCCAATACTTAATCCCA‑GACCAATCCCGCCATAACGGCTGTCCATACGTACCCACGCATCACTCCACTCCCCACATTTACTCTCATCAATACCTGTACATTCATCTTCCACTGCCATAACCGCTCCTTCATACTCTTGCTCATTAATCATCATGtt                                                                                                       >  2:53639/1‑251 (MQ=255)                                                                                                                                          tctttcttCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAATCCCA‑GACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTtgct                                                                                                    >  1:34149/1‑251 (MQ=255)                                                                                                                                                 tcAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAATCCCA‑GACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGc                                                                                             >  1:157652/1‑251 (MQ=255)                                                                                                                                                           acAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAATCCCA‑GACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCACCTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCTTAATGTTGCTGCCTTGCGGTCTGGAAc                                                                                   >  2:389044/2‑251 (MQ=255)                                                                                                                                                                      tGCCGGAAGTCTCTTGCCGGTTTTGCAGGACAAACTGCCCGCGACGCAACTGTGTAATGCGGCTGACAATACTTAATCCCA‑GACCAATCCCGCCACAACGGCTGTCCATACGTACAAACGCTTTACTCACCTCCCCGCATTTACCCTCATCAATACCTGGTCCTTCATCTTCAACTGCCCTGCCCGCTCCGTCATCTTCTTGCCGCTTCATCATAATGTTCCCGCCTTCCGGCCTGTacccctgcgcgctt                                                                        >  2:374335/1‑238 (MQ=255)                                                                                                                                                                             aGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAATCCCA‑GACCAATCCTGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCtcat                                                                                                                                                                             >  1:84272/1‑143 (MQ=255)                                                                                                                                                                                 tctTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAATCCCA‑GACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGtt                                                             >  2:107272/1‑251 (MQ=255)                                                                                                                                                                                   gggcgtttttgaaagaaaaacCTGCCCGTGATTCAACTGTGAAACGGGGCTGACAACACT‑AACCCCAACCCCAACCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTACAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTc                                                           <  1:279982/235‑1 (MQ=255)                                                                                                                                                                                    tGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAATCCCA‑GACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCg                                                          >  1:58733/1‑251 (MQ=255)                                                                                                                                                                                      ccGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAATCCCA‑GACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGc                                                         >  1:293080/1‑250 (MQ=255)                                                                                                                                                                                                   aaaaaCTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAATCCCA‑GACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCgc                                             >  1:174818/1‑249 (MQ=255)                                                                                                                                                                                                               tGATGCAACTGTGTAATGCGGCTGACAATACTTAATCCCA‑GACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCACCTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATc                               >  1:565471/1‑251 (MQ=255)                                                                                                                                                                                                                     aaCTGTGTAATGCGGCTGACAATACTTAATCCCA‑GACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTg                         >  1:159529/1‑251 (MQ=255)                                                                                                                                                                                                                     aaCTGTGTAATGCGGCTGACAATACTTAATCCCA‑GACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTg                         >  1:512261/1‑251 (MQ=255)                                                                                                                                                                                                                      ccTGTGTACTGCGGCTGACAATACTTAATCCCA‑GACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTTCTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATTTCCTCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCCGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCCGCAGGGTTCCATCACCCTg                         >  1:383191/2‑250 (MQ=255)                                                                                                                                                                                                                       cTGTGTAATGCGGCTGACAATACTTAATCCCA‑GACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTtgctg                                                                                                   >  1:566806/1‑175 (MQ=255)                                                                                                                                                                                                                                            aCTTAATCCCA‑GACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGctct  >  1:282250/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      AATCTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGTGCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCA‑GACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCT  >  NC_000913/4332069‑4332554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑