Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,320,785 C→T E155K (GAG→AAG)  phnH ← carbon‑phosphorus lyase core complex subunit PhnH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,320,7850CT98.1% 311.5 / NA 107E155K (GAG→AAG) phnHcarbon‑phosphorus lyase core complex subunit PhnH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (47/56);  minor base G (0/2);  total (49/58)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

GTTAAGCTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTCCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAACGGCGGTGCCGGTGGAAAGGGCGTTGAGCTGTTCGCTGGAAATCGCCTCATCCGTCACCGCGAAGGTCGCCTGTTCCGGCTGGCTGACCAGCGGCGCGTTGGTATGAAAACGCAGGCTCTGGTTGA  >  NC_000913/4320538‑4321030
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     
gTTAAGCTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCgg                                                                                                                                                                                                                                                    <  1:541910/251‑1 (MQ=255)
            ttCAGTCTTGGCGACGCTCAGCTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTATCGCCCCCTTTCACGGCACCGCACATCAGCACACCTCCACATGAGTGGTTCGCGAAATAGCCAGCAGGCGCCCGCCACAGATCAGGATCAGGTCGCTGCCGAGCGGGAGCGGAGACGTGC‑GCCCGGTGAGCTCTGGCTGAATGCACTACGACAGCAGCggcgc                                                                                                                                                                                                                                        >  2:440671/1‑248 (MQ=255)
             tCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGc                                                                                                                                                                                                                                        <  1:167508/250‑1 (MQ=255)
              cAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGACTAGCCAGCAGGCGCTCGCCCCAGGTCCGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGcgcgg                                                                                                                                                                                                                                      >  2:159/1‑250 (MQ=255)
              cAATCCGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCg                                                                                                                                                                                                                                       <  1:491535/250‑1 (MQ=255)
                aTCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATc                                                                                                                                                                                                                                    >  1:92929/1‑251 (MQ=255)
                  cTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCAt                                                                                                                                                                                                                                  >  1:454991/1‑251 (MQ=255)
                   tGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGCGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGAGATCAtt                                                                                                                                                                                                                                 >  1:359328/1‑251 (MQ=255)
                   tGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGACAGCTGCGGGGCGCTCagt                                                                                                                                                                                                                                 >  2:501390/1‑249 (MQ=255)
                    gggctgcGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCTCCCGTTCTCGGCAACGTACATCAGCACACCGCCACAGGAGTGGTTCGCGGAATAGCCAGCGGGCGCTCGCCGCAGGTCAGGATCAGTTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACGTCGGCAGCTGCGGAGCGATCAt                                                                                                                                                                                                                                  <  1:561195/243‑1 (MQ=255)
                    gggCTACGCTCAGGTCGGGCAATGCGGTATCGCCCCGGCGTCGGCTCGCTTGCAGGGCGTGGTCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTc                                                                                                                                                                                                                                <  2:454991/251‑1 (MQ=255)
                    gggCGATGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGG‑GCTCGGTGAGTTTGTGCAGCATGCACTTCGGCAGCTGCGGTGAGATCAt                                                                                                                                                                                                                                  <  1:250159/249‑1 (MQ=255)
                       cGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGtt                                                                                                                                                                                                                             <  1:442758/251‑1 (MQ=255)
                         aCGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGttct                                                                                                                                                                                                                           >  1:335318/1‑251 (MQ=255)
                          cGCTCAGTTCGGGCAACGCGGGATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGACGATCGCCTACTCGCCCCCTGTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAACAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGTAATGGAGGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCCCTTCGGCAGCTGCTGAGCGATCATTCGttctg                                                                                                                                                                                                                          <  2:224344/251‑2 (MQ=255)
                          cGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGCACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGCGCGGGAACGGATGCGG‑C‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGttcttc                                                                                                                                                                                                                         >  1:375139/1‑251 (MQ=255)
                           gCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGttcttc                                                                                                                                                                                                                         <  1:394402/251‑1 (MQ=255)
                            cgcAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGTGCGTGGGCGGCGTCGATCGCCTTCCCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCg                                                                                                                                                                                                                        <  2:118322/249‑1 (MQ=255)
                             tCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCgg                                                                                                                                                                                                                       >  1:45170/1‑251 (MQ=255)
                              cAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCg                                                                                                                                                                                                                        <  1:290606/249‑1 (MQ=255)
                               aGTTCTGGAAAATCGGAATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATAGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCTCACCTCCCAATGAGTGGTTCGCGGAATAGCCAGCAGGCGCACGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTAGGAGGGGTCGTGCAGAATGCACTTCGGCAACTGCGGAGCGATGATTCGTTCTTCGGc                                                                                                                                                                                                                      >  2:82860/1‑250 (MQ=255)
                                   ggggCAAAGCGGTATCGCCCTGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACCCCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATAc                                                                                                                                                                                                                 <  2:444947/250‑1 (MQ=255)
                                     ggCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATAccc                                                                                                                                                                                                               <  1:385998/251‑1 (MQ=255)
                                     ggCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTACAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGAACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTAGGGGAGTTCGTGCAGAATGCACATCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATAccc                                                                                                                                                                                                               >  1:521431/1‑251 (MQ=255)
                                         aaccGGTATCGCCTCGGCGCCTGCGCTCTTGCAGGGCGTGTGCGGCGTCGATCGCCTTCCCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATTCCCGCAc                                                                                                                                                                                                           <  2:211547/248‑1 (MQ=255)
                                         aaTCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCCCGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGGTCGTGCAGCATGCACTTCGGCAGCTGCGGAGCGACCATTCGTTCTTCGGCAATACCCGccc                                                                                                                                                                                                           >  2:20569/1‑249 (MQ=255)
                                         aaTCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCAc                                                                                                                                                                                                           >  1:286667/1‑251 (MQ=255)
                                            cGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCAcc                                                                                                                                                                                                          >  2:201275/1‑249 (MQ=255)
                                              gTATCGCCGCGGCTGCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGACCGCCTTCGCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACAGGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGAGATCATGCGTTCTTCGGCAATACCCGCACCAGTa                                                                                                                                                                                                      <  2:242926/251‑1 (MQ=255)
                                                aTCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTaa                                                                                                                                                                                                     <  1:201275/250‑1 (MQ=255)
                                                aTCGCCGCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGAGCATTCGTTCTTCGGCAATACCCGCACCAGTAAg                                                                                                                                                                                                    <  1:477897/251‑1 (MQ=255)
                                                 cgcctccggaggcgcatctccTGCTGGGCGTGTGCCGCGTCCACCGCCGTCTCGCCCCCTTTCGCTGCAACGTACATCAGCCCCCTTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCACGTCACAGGTCATGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACGTCGGCAGCTGCTGAGCGATCATTCGTGCTTCTGCAATACCCGCACAATTAAgg                                                                                                                                                                                                   <  2:1144/235‑1 (MQ=255)
                                                        ggCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTAGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGAGCAAc                                                                                                                                                                                             >  1:490162/1‑250 (MQ=255)
                                                           gTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGc                                                                                                                                                                                         >  1:556635/1‑251 (MQ=255)
                                                           gTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGc                                                                                                                                                                                         <  1:311039/251‑1 (MQ=255)
                                                           gTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGc                                                                                                                                                                                         <  1:156094/251‑1 (MQ=255)
                                                               gCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTcc                                                                                                                                                                                     >  2:72552/1‑251 (MQ=255)
                                                                 tctctTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTccgc                                                                                                                                                                                   >  1:420789/1‑251 (MQ=255)
                                                                 tctctTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTccgc                                                                                                                                                                                   >  1:253306/1‑251 (MQ=255)
                                                                       gCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCGCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTc                                                                                                                                                                              <  2:286667/250‑1 (MQ=255)
                                                                        cAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTGCACGGCAACGTACATCAGCTCACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAg                                                                                                                                                                            <  1:158000/251‑1 (MQ=255)
                                                                          gggCGTGGAAGGCATCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCCGGATAAGGTCGCTGCCGAGCGGGAACGGAGGCGGGC‑GCTCGGTGAGTTCGGGCAGAATGCACTTCGGCAGCTGAGGAGAGATCATTCGGTCATCGGCAATCCCCGCCCAACTACGGACCAACAAGCGTccgccgaccgggc                                                                                                                                                                          >  2:263179/1‑244 (MQ=255)
                                                                            gCGTGGGCGGCGTCGAGCGCCGTATCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACAGGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGAGGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTGCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTg                                                                                                                                                                        <  1:529226/251‑1 (MQ=255)
                                                                             cGTGGGCGGCGTCGATCGCCGTCGCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTgg                                                                                                                                                                       <  1:304876/251‑1 (MQ=255)
                                                                                 ggcggcGTCGAACGCATTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGAGGTTCGCGGAATAGCCAGCAGGCGCTCGCAACAGGCCCGGATCAGGTCGATGCCGCGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCTGAATGCACTTCGGCCGCTGCGGAGCGATCATTCGTACTTCGGCCATACCCGCACCAGTAAGGCGCAACATGCATCCGCCGATCAGGCTGGCGAc                                                                                                                                                                   >  2:509488/1‑251 (MQ=255)
                                                                                    ggcgTCGATCGCCTTCTCGCCCCCTTTCAAGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTg                                                                                                                                                                >  1:162126/1‑251 (MQ=255)
                                                                                       gTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGCCCTGTa                                                                                                                                                              >  1:40179/1‑250 (MQ=255)
                                                                                       gTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGGGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTgaa                                                                                                                                                              >  1:314843/1‑248 (MQ=255)
                                                                                        ccgtccGCCGTCTCGCCCCCTTCCACGGCCACGTACATCAGCCAACCTCCACATGAGTGGTTCGCGGAAGAGCCAGCAGGCGCTCGCCACAGGTCAGGATAAGGTCGATGCCGAGCGGGAACGGATGCGTGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGTGCGATCATTCGTTCTTCGGCAATCCCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTaaa                                                                                                                                                            <  2:143799/246‑1 (MQ=255)
                                                                                         cGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTaaaa                                                                                                                                                           <  1:508461/251‑1 (MQ=255)
                                                                                          gATCGCCTTCTCGCCCCCTTTCACGGCAACGTACCTCAGCAAACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCCGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATCCGTTCTTCGGCAATCACCGCACCAGTAAGGAGCAACATGCTTCCGCCGCTCCGGCTGGCGAACTGTaaac                                                                                                                                                           >  2:463823/1‑249 (MQ=255)
                                                                                          gATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCAAACCTCCACATGAGTGCTTCGCGGCATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGCGCGGGCACGGATGCGGGC‑GATCGGTGAGTTCGTGCAGCATGCACTTCGGCAGCTGAGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGACCGCCGCTCAGGCAGGCGACCTGAAAAAt                                                                                                                                                          >  2:402597/1‑251 (MQ=255)
                                                                                                      gCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCAAAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGAACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCAcc                                                                                                                                              >  1:340160/1‑251 (MQ=255)
                                                                                                       cccccTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCAccc                                                                                                                                             <  1:296997/251‑1 (MQ=255)
                                                                                                       cccccTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCAccc                                                                                                                                             <  1:453653/251‑1 (MQ=255)
                                                                                                        ccccTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCg                                                                                                                                            >  1:101161/1‑251 (MQ=255)
                                                                                                               cACGGCAACTTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCgg                                                                                                                                     <  1:63910/251‑1 (MQ=255)
                                                                                                                 cGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGGTCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTAGCACCCGCTTCCGgcg                                                                                                                                   >  2:198509/1‑251 (MQ=255)
                                                                                                                   gCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCCCAGGTCAGGCTCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCCGAATGCACTTCGGCAGCTGCGGCGCGATCATTCGTTCTTCGGCAATACCCGCCCCAGTACGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGgcgcc                                                                                                                                 >  2:61375/1‑250 (MQ=255)
                                                                                                                        gTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAAcggc                                                                                                                            <  1:202259/251‑1 (MQ=255)
                                                                                                                        gTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAAcggc                                                                                                                            <  1:444970/251‑1 (MQ=255)
                                                                                                                         tACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAAcggcg                                                                                                                           >  1:33250/1‑251 (MQ=255)
                                                                                                                         tACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAAcggcg                                                                                                                           >  1:138778/1‑251 (MQ=255)
                                                                                                                             ccAGCACCCCTCCACAGGAGGGGTCCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTAGATGCCGAGGGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCCCTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAACGGCGGTGc                                                                                                                       <  2:480988/250‑1 (MQ=255)
                                                                                                                               aGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAACGGCGGTggcg                                                                                                                     >  1:117733/1‑248 (MQ=255)
                                                                                                                               aGCACACCTCAACATGAGTGATGCGCGGAATAGCCCGCCTGCGCTCGCAACAGGACCGGATCAGGTCGATGCCGCGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCATGCAGAATGCACTACGTCAGCTGCGGAGCGATCCTTCGTTCTTCGTCAATACCCGCCCCAGCAAGGCGCACCATGCGTCCGCCGCTCGGGccggggccctg                                                                                                                                                                >  2:62343/1‑198 (MQ=255)
                                                                                                                                gCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAACGGCGGTGCCgg                                                                                                                    <  1:501390/251‑1 (MQ=255)
                                                                                                                                 cacaCCTCCTCATGCGTTGTTCGCGGAATAGCCCGCGGGCGCTCGCCACAGGCCAGTATCAGGTCGATGCCGAGTGGGACCGGATGCGGGC‑GCGCGGTGAGGTCGTGCGGAATGCACTTCGGCAGCTGCGGAGCGATCTTTCGTTCTGCGGCAATACCCGCCCCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCCCCCGCTTCCGGCGCAACGGCGGTGCCGGt                                                                                                                   <  2:452002/251‑1 (MQ=255)
                                                                                                                                 cacaCCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAACGGCGGTGCCggg                                                                                                                   >  1:2814/1‑250 (MQ=255)
                                                                                                                                 cacaCCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAACGGCGGTGCCggg                                                                                                                   >  1:423416/1‑250 (MQ=255)
                                                                                                                                      cTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGACCTTCGGCAGCTGCGGCGCGATCATTCGGTCTTCGGCAATCCCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCCGGCTGGCGTCCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAACGGCGGAGGCGGTggga                                                                                                               >  2:155259/1‑248 (MQ=255)
                                                                                                                                          caaTGAGGGGTTCGCGGAGTAGCCAGCAGGCGCGCCCCACAGGGCAGAACCAGGTCGATGCCGCGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTCCGGCAATACCCGCTCCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAACGGCGGTGCCGGTGGAAAGGGc                                                                                                          <  2:138778/249‑1 (MQ=255)
                                                                                                                                           caTGAGTGGTTCGCGGAATAGCAAGCAGGCGCTCGCCACAGATCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTCAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCAGGCGCacccgacgtcccgggtggaggggcg                                                                                                         >  2:125920/1‑227 (MQ=255)
                                                                                                                                             ggaggggTTCGCGGAATAGCCGGCAGGCGCTCGCCACAGGGCAGGAGCAGGTCGAGGCCGACCGGGAACGTATGCGGGC‑GCTCGGGTATTTCGTGCAGAATGCTCTTCGGCAGCTGCGGATCGATCATTCGTTCTTCGGCAATCCCCGCCCCGGTAAGGCGCAACAGGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAACGGCGGTGCCGGTGGAAAGGGCGtt                                                                                                       <  2:134373/246‑1 (MQ=255)
                                                                                                                                                  ggTTCGCGGAAGAGCCAGCAGGCGGCCGCCCAAGTTCAGGATCAGGGCGATGCCGAGCGGGAACGGAGGTGGGC‑GCTAGGTGAGGTCGTGCAGAAGGCACTTCGGCAGCTGCGGGGCGATCATTCGTTCTTCGGCAATTCCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAACGGCGGTGCCGGTGGAAAGGGCGTTGAGCt                                                                                                  <  2:372454/251‑1 (MQ=255)
                                                                                                                                                         gtaaTAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAACGGCGGTGCCGGTGGAAAGGGCGTTGAGCTGTTCGCt                                                                                           <  1:382412/249‑1 (MQ=255)
                                                                                                                                                          gAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAACGGCGGTGCCGGTGGAAAGGGCGTTGAGCTGTTCGCTg                                                                                          <  1:194070/251‑1 (MQ=255)
                                                                                                                                                                    cagGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGCACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAACGGCGGAGCCGGAGGAGAGGGCGTTGCGCCGTTCGccgggcatcgccc                                                                                >  2:133035/1‑239 (MQ=255)
                                                                                                                                                                     agGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGAGGGAACGGATGTGGGC‑GCTCGGTGAGTTCGTGCAGAATGCATTTCGGCAGCTGCGGATCGATCATTCGTTCTTCGGCAATACCCGCTCCAGTAAGGCGCAACTTGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAACGGCGGTGCCGGTGGAAAGGGCGTTGAGCTGTTCGCTGGAAATCGCCTc                                                                               <  1:65404/251‑1 (MQ=255)
                                                                                                                                                                          cTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAACGGCGGGGCCGGGGGAAAGGGCGTTGAGCTGTTCGCTGGAAATCGCCTCATCCg                                                                          >  1:477562/1‑251 (MQ=255)
                                                                                                                                                                                acaGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAACGGCGGTGCCGGTGGAAAGGGCGTTGAGCTGTTCGCTGGAAATCGCCTCTTCCGTCACcg                                                                    >  1:387783/1‑251 (MQ=255)
                                                                                                                                                                                  aGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCCCTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAACGGCGGTGCCGGTGGAAAGGGCGTTGAGCTGTTCGCTGGAAATCGCCTCATCCGTCACcgcg                                                                  <  1:93981/251‑1 (MQ=255)
                                                                                                                                                                                   ggtctggatcggggcgcggCCGAGCGGGAACGGATGCGGGC‑GCCCGGTGAGTTCGTGCAGAATGCACTTCGGCCGCCGCGGAGCGATTATTCGTTCTTCGGCAATACCCGCCCCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTGCCGGCGCAACGGCGGTGCCGGTGGAAAGGGCGTTGAGCTGTTCGATGGAAATCGCCTCATCCGTCACcgcg                                                                  <  2:359328/232‑1 (MQ=255)
                                                                                                                                                                                                 cGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAAGCAGCGTCGCACCCGCTTCCGGCGCAACGGCGGTGCCGGTGGAAAGGGCGTTGAGCTGTTCGCTGGAAAt                                                                                     <  1:326834/217‑1 (MQ=255)
                                                                                                                                                                                                   gtGCCGGGCGGGAGCGGATGCGGGG‑TCTCGGGGAGTTCGTGCAGAACGGACTTCGCCAGATGCGGAGCGATCATGCTTTCTTCGGCAATACCCGCACCACTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAACGGCGGTGCCGGTGGAAAGGGCGTTGAGCTGTTCGCTGGAAATCGCCTCATCCGTCACCGCGAAGGGCGCCTGTTCCgg                                                 <  2:140941/250‑1 (MQ=255)
                                                                                                                                                                                                       cGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAACGGCGGTGCCGGGGGGAAGGGCGGTGGGCTGTTCGCTGGAAATCGCCTCATCCGTCACCGCGGAGGGCGCCCGGTCCggctgg                                             >  1:359724/1‑251 (MQ=255)
                                                                                                                                                                                                        gAGCGGGACCGGTTGCGGGCCGC‑CGGTGAGTTGGTGGAGAAGCCGCCGCGGCAGCTGCGGAGCAGTCATTCGTTCTTCGGCAATACCGGCCCCAGTAAGGCGCCACAGGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAACGGCGGTGCCGGTGGAAAGGGCGTTGAGCTGTTCGCTGGAAATCGCCTCATCCGTCCCCGCGAAGGTCGCCTGTTCCggctggc                                            <  2:40179/251‑1 (MQ=255)
                                                                                                                                                                                                          gCGGGAACGGATGCGGGC‑GCTCGGGTAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCTCCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAACGGCGGTGCCGGTGGAAAGGGCGTTGAGCTGTTCGCTGGAAATCGCCTCATCCGTCACCGCGAAGGTCGCCTGTTCCGgctggctg                                          <  1:509603/251‑1 (MQ=255)
                                                                                                                                                                                                           ggggACCGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAACGGCGGTGCCGGTGGAAAGGGCGTTGAGCTGTTCGCTGGAAATCGCCTCATCCGTCACCGCGAAGGTCGCCTGTTCCGGCTGGCTGa                                         <  1:159/250‑1 (MQ=255)
                                                                                                                                                                                                            gggAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAACGGCGGTGCCGGTGGAAAGGGCGTTGAGCTGTTCGCTGGAAATCGCCTCATCCGTCACCGCGAAGGGCGCCTGTTCCGgctggctggc                                        >  1:42578/1‑249 (MQ=255)
                                                                                                                                                                                                                      gCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAACGGCGGTGCCGGTGGAAAGGGCGTTGAGCTGTTCGCTGGAAATCGCCTCATCCGTCACCGCGAAGGTCGCCTGTTCCGGCTGGCTGACCAGCGGcgcg                              <  1:522439/251‑1 (MQ=255)
                                                                                                                                                                                                                            cgatgggttaGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCTTTCTTCGGAAATACCCGCACCAGTAAGGCGCATCATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAACGGCGGTGCCGGTGGAAAGGGCGTTGAGCTGTTCGCTGGAAATCGCCTCATCCGTCACCGCGAAGGTCGCCTGTTCCGGCTGGCTGACCAGCGGCGCGTTGGt                         <  1:451616/242‑1 (MQ=255)
                                                                                                                                                                                                                                cGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAACGGCGGTGCCGGCGGAGAGAGCGGTGGGGTGTTTGCTGGGAATCGCCTCATCCGCCCCCGCGAAGGTCGCCTTTTTCGGCTGGCCGACCCGCGgcgccgtgggttgg                     >  2:203632/1‑241 (MQ=255)
                                                                                                                                                                                                                                cGGTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTGCCGGCGCAACGGCGGTGCCGGTGGAAAGGGCGTTGAGCTGTTCGCTGGAAATCGCCTCATCCGTCACCGCGAAGGTCGCCTGTTCCGGCTGGCTGACCAGCGGCGCGTTGGTAt                       <  1:132096/249‑1 (MQ=255)
                                                                                                                                                                                                                                  gTGAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAACGGCGGTGCCGGTGGAAAGGGCGTTGAGCTGTTCGCTGGAAATCGCCTCATCCGTCACCGCGAAGGTCGCCTGTTCCGGCTGGCTGACCAGCGGCGCGTTGGTATGaaa                   <  1:361019/251‑1 (MQ=255)
                                                                                                                                                                                                                                    gAGTTCGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAACGGCGGTGCCGGTGGAAAGGGCGTTGAGCTGTTCGCTGGAAATCGCCTCATCCGTCACCGCGAAGGTCGCCTGTTCCGGCTGGCTGACCAGCGGCGCGTTGGTATGAAAAc                 <  1:182442/251‑1 (MQ=255)
                                                                                                                                                                                                                                     cagtCGTGCAGAATGCCCTGCGGCTTCAGCGGCGCTAACATTTGTTCTTCGGCAATCCCCGCACCAGTACGGCGCTACATGCGGCCGCCGCGCAGGCTGGCGACCTTTAAAATCAGCGTCGCACCCGCTTCCGGCGCAACGGCGGTGCCGGTGGAAAGGGCGTTGAGCTGTGCGCTGGAAATCGCCTCATCCGTCTTCGCGAAGGTCGCCTGTTCCGGCTGGCTGACCAGCGGCTCGTTGGTATGAAAACg                <  2:350441/248‑1 (MQ=255)
                                                                                                                                                                                                                                         cGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAACGGCGGTGCCGGTGGGAAGGGCGGTGAGCTGTTCGCTGGGAATAGCCTAATCCGTCAACGCGAAGGGCGCCTGTTCCGGCTGGCTGACCTGCGGCGGGTTGGTaaggaaaaggcgg            >  1:175440/1‑239 (MQ=255)
                                                                                                                                                                                                                                         cGTGCAGAATGCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAACGGCGGTGCCGGTGGAAAGGGCGTTGAGCTGTTCGCTGGAAATCGCCTCATCCGTCACCGCGAAGGTCGCCTGTTCCGGCTGGCTGACCAGCGGCGCGTTGGTATGAAAACGCAg             <  1:319217/250‑1 (MQ=255)
                                                                                                                                                                                                                                                   gCACTTCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAACGGCGGTGCCGGTGGAAAGGGCGTTGAGCTGTTCGCTGGAAATCGCCTCATCCGTCACCGCGAAGGTCGCCTGTTCCGGCTGGCTGACCAGCGGCGCGTTGGGATGAAAACGCAGGCTCTGGtttg  >  1:175339/1‑249 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     
GTTAAGCTGCTGTTCAATCTGGGCGACGCTCAGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGGCGCTCGCCACAGGTCAGGATCAGGTCGATGCCGAGCGGGAACGGATGCGGGC‑GCTCGGTGAGTTCGTGCAGAATGCACTCCGGCAGCTGCGGAGCGATCATTCGTTCTTCGGCAATACCCGCACCAGTAAGGCGCAACATGCGTCCGCCGCTCAGGCTGGCGACCTGTAAAATCAGCGTCGCACCCGCTTCCGGCGCAACGGCGGTGCCGGTGGAAAGGGCGTTGAGCTGTTCGCTGGAAATCGCCTCATCCGTCACCGCGAAGGTCGCCTGTTCCGGCTGGCTGACCAGCGGCGCGTTGGTATGAAAACGCAGGCTCTGGTTGA  >  NC_000913/4320538‑4321030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑