Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,320,079 C→T Q195Q (CAG→CAA)  phnI ← carbon‑phosphorus lyase core complex subunit PhnI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,320,0790CT96.9% 288.1 / NA 100Q195Q (CAG→CAA) phnIcarbon‑phosphorus lyase core complex subunit PhnI
Reads supporting (aligned to +/- strand):  ref base C (1/1);  major base T (46/49);  minor base G (0/3);  total (47/53)
Fisher's exact test for biased strand distribution p-value = 2.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCG  >  NC_000913/4319838‑4320325
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       
ccAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGTGTCGATAAACCCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCTTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCaaa                                                                                                                                                                                                                                                <  1:537128/251‑1 (MQ=255)
         aGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGggagg                                                                                                                                                                                                                                         >  1:45128/1‑249 (MQ=255)
           catctcgTGAAGTGCGGCGGCTCACCCGGCAGGTCGATAAAACCGTTAACCATTTCACCCTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCCTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCg                                                                                                                                                                                                                                     <  2:455756/246‑1 (MQ=255)
             gggcgTGAAGGGCGGCGGCTCACCCTGCGGGTCGATGAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTg                                                                                                                                                                                                                                   <  2:561618/249‑1 (MQ=255)
             gcgcgTGAAGAGCGGCGGATCACCCGGCGGGTCGATAAAACCGTTCACCCATTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAACTCCCAGCTCTTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGAACTCGCCTGCGAAAGGG‑GGATTGCGCCCGTAAACGCGATGGGTGGGGGTGGGCAGCGCCACCAAATAGCATTCGTCGCCGCGCATCAATTGGAGCAAACaggagggggc                                                                                                                                                                                                                                     >  2:384702/1‑245 (MQ=255)
                cgTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGGGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCg                                                                                                                                                                                                                                     >  1:455756/1‑246 (MQ=255)
                          ggcggcTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGACGTTCGCCGACCTTCACCGCAAATACCAACTCTTCCGGCACAATCGACACGTCGA‑TAAAGCCGCTGCCGATCTCGCCTGCGAACGGG‑TGGTTGCGCCCGTAAACGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAACCATCGACGCCGAGCATCCATTGCTGCAAACGGGAGGAGCggccgcaggggaaacc                                                                                                                                                                                                                      >  1:545636/1‑236 (MQ=255)
                                ccgcccggcggagccaTAAACCCCTTAACCATTTCACCCTCAGGCAACAGCATTTCGCCGATGCTCACCGCAGATCCCAGCTCCACCTGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCGCGCCTGCGAACGGG‑TGAGTGCGCCCGTAACCGCGTTGGGTGTAGTAGGCCAGCGCGAGCAAATAGCCGTCGTCGCCGCGCATCAATTGCTGCAAACGTGAGGAGCGTGAGCAGTTGTAA‑ACcggcgg                                                                                                                                                                                                               <  2:229905/236‑1 (MQ=255)
                                     cggcggGGCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTTTTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGc                                                                                                                                                                                                          <  1:566634/251‑1 (MQ=255)
                                      ggggggTCGTTCAAACCGTGAACCATTTCCCACTCAGTCATCAGCAGTTCGCTGACGTTCTCCGCAAATCCCAGCTCTTCCGGCACAATCGGCTCGTCGA‑TATTGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAAGTGCTGCAAACGGTAGGAGCGTGAGCAGGTTTAA‑ACCGGCGGCGTGCg                                                                                                                                                                                                         <  2:530710/248‑1 (MQ=255)
                                      ggcggGTCGATAAAACCGTTAACCATTTCCCACCCAGGCATCAGCAGCTCGCCGACGTTCACCGCAAAACCCAGCTCGTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCg                                                                                                                                                                                                         <  1:507951/251‑1 (MQ=255)
                                      ggcggGTCGATAAAAACGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGCCACGTCCA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGGGTG‑TTGCGCCCGTAACCGCGTTGGGTGGGGTAGGCCAGCGCCAGCAACTAGCCTTCGTCGCCGCGCATCACTTGCTGCAAACGGGAGGAGCGTGAGCCGGGGTAA‑ACCGGCGGCGTGCg                                                                                                                                                                                                         >  2:156757/1‑251 (MQ=255)
                                        ggggccGATAAAACCGTTACCCATTTCACACTCAGTCATCAGCAGTTCGCCGCCGTTCACCGCAAATCCCATCTCTTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTGCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCggg                                                                                                                                                                                                       <  2:171110/246‑1 (MQ=255)
                                         ggggcgaaaaaaCCGTTAACCATTTCCAACTCAGTCATCAGCAGTTCGCCGACGTTCTCCGCAAATCCCAGCCCTTCCGGCACAATCTACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGt                                                                                                                                                                                                      <  2:519449/243‑1 (MQ=255)
                                             cGATAAAACAGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCACGGGTAA‑ACCGGCGGCGTGCGGGGGATg                                                                                                                                                                                                  >  2:392118/1‑251 (MQ=255)
                                               aTAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGACGACGTTCACCACAAATCCCAGCTCTTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGCTCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGCGGAGGCCAGCGCCAGCAAATAGCCTTAGTCGCCGCGCATCAGTTGATGCACACGGGAGGAGCGTGCGCAGGGGACA‑AACGGCGGAGTGCGAGTGCTGTc                                                                                                                                                                                                >  2:307033/1‑251 (MQ=255)
                                                 aaaaCCGTGAACCCTGTCACCCGCAGTCAGCATCAGGTCGCCGTCGGTCGCCGCAAATCCCAGCTCATCCGGCTCAATCGACACGTAGA‑TATAGCCGCTTCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTGCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCa                                                                                                                                                                                               <  2:186348/250‑1 (MQ=255)
                                                  aaaCCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCCCAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTACGAACGGG‑GGATGGCGCCCGTCACCGCGTTGGGGGGAGTAGGCCAGCGCCCGCAAATAGCCTACGTCGCCGCGCATCAATTGCTGCAAACGGGCGGACCGGGAACAGGGGTTA‑ACCGGCGGCGATCGGGTGATGTCATc                                                                                                                                                                                             >  2:396633/1‑251 (MQ=255)
                                                     ccGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACTTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGTGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCgg                                                                                                                                                                                          <  1:434923/251‑1 (MQ=255)
                                                        ttAACAATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCCACTCCCAGCTCTTCCGGCACAATCGACACGTCGA‑TATAGCAGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATggactccggctg                                                                                                                                                                                       >  2:319363/1‑240 (MQ=255)
                                                             cATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTgcgc                                                                                                                                                                                  >  1:430286/1‑251 (MQ=255)
                                                             cATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTgcgc                                                                                                                                                                                  >  1:191077/1‑251 (MQ=255)
                                                               tttCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCATTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGGAA‑ACCGGAGGCGTGCGGGGGATGTCATCCGGCTGTGcgccg                                                                                                                                                                                >  2:69196/1‑251 (MQ=255)
                                                               tttCACAATCAGTCATCAGCAGTTAGCCGACGTTCACCGCAACTCCCAGCTCTTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCACGTACCCGCGTTGGGTGGCGTAGGCCAGCGCCAGCCAATAGCCTTCGCCGCCGCGCATCACTTGCTGCCAACGGGAGGAGCGTGATCCGGGGTAA‑ACCGGCGGCGTGCGGGTGGTATCCTCCGGCTGTGcgccg                                                                                                                                                                                >  2:201400/1‑251 (MQ=255)
                                                                      cTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGATCTTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCct                                                                                                                                                                         >  1:507353/1‑251 (MQ=255)
                                                                       tCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCctc                                                                                                                                                                        >  1:563086/1‑251 (MQ=255)
                                                                         catcctcaGCTGTTCGCCGACGTTCACCGCAAAGCCCAGCTCTTCCGGCACAATCGACACGGCTA‑TTTCGCCGCTGCGGATCTCGCCTGCGACCGGG‑TGATGGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCAGCCGGCTGTGCGCCGCTATCctcta                                                                                                                                                                      <  2:417580/246‑2 (MQ=255)
                                                                         aGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCtt                                                                                                                                                                      <  1:315981/251‑1 (MQ=255)
                                                                          gTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTTGGTGGAGTAGTCCAGCGCCAGCAAATAGCCTTCTGCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCATGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTc                                                                                                                                                                     <  1:537603/251‑1 (MQ=255)
                                                                          gTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTc                                                                                                                                                                     <  1:516569/251‑1 (MQ=255)
                                                                              tcaGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAAc                                                                                                                                                                 >  2:57252/1‑251 (MQ=255)
                                                                                     gtCGCCGGCGTTCACCGCAAAGCCCAGCTCTTCCGGCACAATCGACACGGCGA‑GATAGCCGCGGCGGATACCGCCGGCGAACGGG‑TGAGTGCGCCCGTATCCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCAGCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATTCTCTTCAAACTTCGCCa                                                                                                                                                          <  2:100491/250‑1 (MQ=255)
                                                                                       cgccgcCGTTCACCGCAAAGCCCAGCTCTTCCGGCACAATCGACACGGCGA‑TATAGCCGCTGCGGATCGTGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGCGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAACCTTCGCCAGc                                                                                                                                                        <  2:355078/251‑1 (MQ=255)
                                                                                       cGCCGACGTTCCCCGCAAACCCAAGCTCTGCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGAGTGCGCCCGTAAGCGCGGTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAga                                                                                                                                                        <  2:212471/251‑2 (MQ=255)
                                                                                       cGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGc                                                                                                                                                        >  1:291037/1‑251 (MQ=255)
                                                                                         ccGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGGGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCGCTTCAAACTTCGCCAGccc                                                                                                                                                      >  2:481725/1‑251 (MQ=255)
                                                                                               ttCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGc                                                                                                                                                        <  1:177993/243‑1 (MQ=255)
                                                                                               ttCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGGGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGcagagcgg                                                                                                                                                 >  2:177993/1‑243 (MQ=255)
                                                                                               ttCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCACCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGAcg                                                                                                                                                >  1:428585/1‑251 (MQ=255)
                                                                                                 cACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGAcgcg                                                                                                                                              <  1:307937/251‑1 (MQ=255)
                                                                                                 cACCGCAAATCCCAGCTCTGCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGAcgc                                                                                                                                               <  1:555893/250‑1 (MQ=255)
                                                                                                     gCAAATCCCAGATCTTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCCGCAAATAGCCTTCGCCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAACACCG‑CGGCGTGCGGGCGATGACATACGGCTGTGCGCCGCTCTCCTCTTCAAACTTCGCCAGCCCCTGACGCGcc                                                                                                                                            >  2:72569/1‑249 (MQ=255)
                                                                                                        aaTCCCAGCTCTTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCcagc                                                                                                                                         >  1:131111/1‑249 (MQ=255)
                                                                                                           cccAGCTCTTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGc                                                                                                                                     <  1:426309/250‑1 (MQ=255)
                                                                                                            ccAGCTCTTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTg                                                                                                                                   >  1:406531/1‑251 (MQ=255)
                                                                                                            caaGCTCTTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGAAACCGCGTTGGGGGGGGAAGGCCAGCGCCAGCAATTAGCCTTCGCCGCCCCGCATAAATGGTTCCAAACGGGAGGAGGGTGAGCAGGGGTAA‑CCCGGCGGCGTGCGGGGGATGCCAGCGGGCGGGCCGCCGCTATCCCCTTCAAACTTCGACGGCCCCTGACGGCCCAGCCGGCTg                                                                                                                                   >  2:106100/3‑251 (MQ=255)
                                                                                                             cAGCTCTTCCGGCAAAATCGACACGGCGA‑TATAGCAGCTGCGGAGCGCGACTGCGCACGGG‑TGATTGGGCCCGTAACCGCGTTGGGTGGATTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCAGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGGGGGCCGCTAACCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGa                                                                                                                                  <  2:130068/251‑1 (MQ=255)
                                                                                                                 cctgccGGCACAAGCGCCCCGTCGA‑CATAGCCGCTGCGGATCCCGCCTGCGAACGGG‑TGATTCCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAACCTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAAc                                                                                                                              <  2:493440/247‑1 (MQ=255)
                                                                                                                  ctTCCGGCACAATCGAAACGTCGA‑TAGAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGaaaa                                                                                                                               <  1:315373/249‑1 (MQ=255)
                                                                                                                    tccggcacaactgacgtgccGA‑TATAGCCGCTGCGCATCTCGCCTGCGAACGGG‑GTATTGCGCGCGTATCCGTGTTGGGTGGAGTAGGCCGGCGCCAGGAAATAGCCTTCGTCGCCGCGCATCAATTGCTGGAAGCGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGGGGCGTGCGGGTGATGTCATCCGGCTGTGCGCGGCTATCCTCGTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGt                                                                                                                            <  1:249476/231‑1 (MQ=255)
                                                                                                                    tCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAAc                                                                                                                              <  1:243852/248‑1 (MQ=255)
                                                                                                                     ccGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑CGATTGCGCCCGTAACCGCGTTGGGCGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCCGCCCCTGTAGCGCCAGCAGGCTGCAAACGTgc                                                                                                                          >  1:114194/1‑251 (MQ=255)
                                                                                                                       ggCACAATCGACCCGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAgggg                                                                                                                                                                                                                           >  1:138190/1‑153 (MQ=255)
                                                                                                                       ggCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGAGTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAgggg                                                                                                                                                                                                                           <  2:138190/153‑1 (MQ=255)
                                                                                                                        gCACACTCGACACGTCGAGTA‑AGCCGCTGCGGATCTCGCCTGCGAACGGT‑TGCTTGCGCCCGTAACCGCGTTGGGTGGGGTAGGCCAGCGCCAGCAAATAGCCTTCGGCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCGCTGCAAACTTCGCCATCCCCGGACGCGCCAGCAGGCTGAAAACGTgcggc                                                                                                                       <  2:78162/251‑1 (MQ=255)
                                                                                                                        gCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGAt                                                                                                                                                                                                   >  1:356145/1‑175 (MQ=255)
                                                                                                                        gCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGAt                                                                                                                                                                                                   <  2:356145/175‑1 (MQ=255)
                                                                                                                           caATCGACCCGTCAA‑CTTAGCCGCGGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGTAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGAc                                                                                                                    <  2:261381/251‑1 (MQ=255)
                                                                                                                                gACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGgcgg                                                                                                               >  2:252760/1‑249 (MQ=255)
                                                                                                                                 acacGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGgctgc                                                                                                              >  1:324479/1‑251 (MQ=255)
                                                                                                                                 acacGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCACCAGCCCCTGACGCCCCAGCAGGCTGAAAACGGGCGGCTAAGgctgc                                                                                                              >  2:144224/1‑251 (MQ=255)
                                                                                                                                  cacGTAGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGAACGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGACGCGAATCCATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAG‑AACGGCTGCGGGCGGGGGATGTCATACAGCTGCGGGCCGCTATCCTCGTCAAACTACGCAAGCCCCTGACGCGCCAGCGGGCTGAAAACGGGCGGCGAAGgctgcg                                                                                                             >  2:300736/1‑250 (MQ=255)
                                                                                                                                   ccGTCGC‑TATAGCCGCTGCGGACATCGCATGCGAACGGG‑TGATTGCGCCCGTAACCGCGGTGGGTGGGGTAGGCCAGCGCAAGCAAATAGCCTTCGTCGCCGCGCATCAATGGCTGCAAACGGGAGGAGCGTGAGCAGGGGGAA‑ACCGGCGGCGGGCGGGTGATGTCATCCGGCGGTGCGCCGCTATCCTCTCCAAGCTCCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACTTGCGGCGACGGctgctg                                                                                                            <  2:125832/250‑1 (MQ=255)
                                                                                                                                    ccctcgaatgtgCCGCTGCGGAGCCCGCCGGCGAACGGT‑GGATTGCGCCCGGAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCTGTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGGCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGctgctg                                                                                                            <  2:513924/240‑1 (MQ=255)
                                                                                                                                        gA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTgc                                                                                                                          >  1:388244/1‑232 (MQ=255)
                                                                                                                                        gA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGc                                                                                                       >  1:268166/1‑251 (MQ=255)
                                                                                                                                         a‑tatAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAACCTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCt                                                                                                      <  1:332555/251‑1 (MQ=255)
                                                                                                                                              aGCCGCTGGGGTCCTCGCCTGCGAACGGG‑GGTTTTCGCCCGTAACCGCGGTGGGTGGATGAGGCCAGCGCCAGAAAATATCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGGGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTc                                                                                                   <  2:406531/250‑1 (MQ=255)
                                                                                                                                              aGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTcg                                                                                                  <  1:156757/251‑1 (MQ=255)
                                                                                                                                                ccGCTGCGGATCGCGCCTGCGAACGGG‑TGATTGCGCCCTGAATCGCGTTGGGTGGAGGAGGCCAGCGCCAGCAAAGAGCCTTCGTCGCCTCGCATCAAGTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTAATCCGGCTTTGCGCCGCTATCCCCTTCAAACTGCGTCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTTCTGTTCGCTGttg                                                                                                  <  2:420831/249‑3 (MQ=255)
                                                                                                                                                           ctcGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCCGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGCGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGcc                                                                                     >  1:254313/1‑250 (MQ=255)
                                                                                                                                                            tcGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGAAGGCCAGCGCCAGCACATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGCGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGCGATGTCATCCGGCTGTGCGCCGCTCTCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCCGCAGGCGGACAACGTGCGGCCACCGCTGCTGTTCGcggacgccggggtgcacggg                                                                                    >  2:522584/1‑232 (MQ=255)
                                                                                                                                                             cGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGc                                                                                      <  1:69196/248‑1 (MQ=255)
                                                                                                                                                               ccggagAACGGG‑GGATGGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCgg                                                                                 <  2:253093/246‑1 (MQ=255)
                                                                                                                                                               ccTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCgg                                                                                 <  1:370066/251‑1 (MQ=255)
                                                                                                                                                                cTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGAGGAGTCGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGTGGAGCGTGAGAAGGGGTAA‑ACCGGCGGCGTGCGGGAGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGCAGCCGGCTGCTGTTCGCTGTcggcgggggaccgcgagtgg                                                                                >  2:58048/1‑237 (MQ=255)
                                                                                                                                                                    gAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGc                                                                                                                                                                                                                                      <  1:518145/98‑1 (MQ=255)
                                                                                                                                                                    gAACGGG‑TGATTGCGCCCGTAACCGAGTTGGGTGGCGTAGGCCAGAGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCACACGGGAGGAGc                                                                                                                                                                                                                                      >  2:518145/1‑98 (MQ=255)
                                                                                                                                                                      aCGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCg                                                                          <  1:319363/251‑1 (MQ=255)
                                                                                                                                                                            ggATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGtt                                                                     <  1:272621/250‑1 (MQ=255)
                                                                                                                                                                             gATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGGGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGGGGTCAGCGCGGGCGCTTCGCCggtt                                                                    >  2:108192/1‑248 (MQ=255)
                                                                                                                                                                                tGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGcc                                                                 >  1:64472/1‑251 (MQ=255)
                                                                                                                                                                                   gcCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGGGGTCAGCGTCGGCGCTTCGCCGTTTGCcagc                                                              >  1:166392/1‑251 (MQ=255)
                                                                                                                                                                                           ccGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTaaa                                                      <  1:392118/251‑1 (MQ=255)
                                                                                                                                                                                           ccGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTaaa                                                      <  1:57252/251‑1 (MQ=255)
                                                                                                                                                                                                  ggggggagcagtccagcgccagcAATGTGCCTCCGTCGCCGCCCATCAATTGCTGCAACCGGGAGGAGCGTGTGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGTTGCCAACCGGCTGGGCGCCCCTACCCCCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGGCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGTCAGCAGGGTAAAATCGAGc                                               <  2:64472/239‑1 (MQ=255)
                                                                                                                                                                                                       gAGTAGGCCAGCGCCAGCAAATAGCATTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCg                                          <  1:384702/251‑1 (MQ=255)
                                                                                                                                                                                                           aGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTc                                                                                                         <  1:568775/184‑1 (MQ=255)
                                                                                                                                                                                                           aGGCCAGCGCCAGCAAATAGCCTTCGTAGCCGCGCATCAATTTCTGAAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTAAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCCGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGGTgg                                      >  1:215388/1‑251 (MQ=255)
                                                                                                                                                                                                            ggCCAGCTCCAGCAAATAGCCGTCGTCGCCGCGCAGCAATTGCTGCAAGCGGGAGGAGCGTGAGCAGGGGTAA‑CCCGGCGGCGTGCGGGTGATGTCATCCGGCGGTGCGCCGCTATCCTCTTCAAACTTCGACAGCCCCTGCCGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATggg                                     <  1:311675/251‑1 (MQ=255)
                                                                                                                                                                                                              ccctctacagcAAATAGCGTCCGGCGCCTCGCACCAAGTGTGGCAATCGGGAGGAGAGCGAGCAGGGGTAA‑CCCGGCGGCGGGCGGTTGATGCCATACGGCTTGGCGCTGCTATCCACATCAAACTGCGCCAGCCCCTGCCGCGCCCGCTGGCTGAAAACGTGCGGCGACGGCTGCTGGTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGgtg                                   <  2:324094/244‑1 (MQ=255)
                                                                                                                                                                                                               cagcgccagcAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGgtgt                                  <  1:478070/251‑1 (MQ=255)
                                                                                                                                                                                                                       gcAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGGGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGCTGGGGGTAGTCGt                            >  1:447177/1‑249 (MQ=255)
                                                                                                                                                                                                                          aaTAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTc                                                                           <  1:361843/199‑1 (MQ=255)
                                                                                                                                                                                                                                cTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGGAAAATCGAGCAGGCGGGGGGGGTAGTCGTAGGGTGGGcc                  >  1:101058/1‑250 (MQ=255)
                                                                                                                                                                                                                                    gtcgCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAagc              >  1:155194/1‑250 (MQ=255)
                                                                                                                                                                                                                                    gtcgCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGGGTAGTCGTAGGTTGGGCCAagc              >  1:544449/1‑250 (MQ=255)
                                                                                                                                                                                                                                      cgccgcGCATCAATGGCGGCAACCGGGAGGAGCGTGGGCAGGGGTAA‑ACCGGCGGCGGGGGGGGGATGCCATCCGGCTGTGCGCCGCTATCCCCTTCAAGCTCCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGGTGGGCCAagcagc           <  2:324479/251‑1 (MQ=255)
                                                                                                                                                                                                                                      cgcagcgcATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGCAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCATGACGCGCAAGCAGGCTGAAAACGTACGGCGACGGCTGCTGTTCGCTCTCGGCGGTGGTGAACGACGGAGCTTCGCCGTTTGCCAGCAGGGGAAAAACGAGAAGGCGCTGGGGGTAGTCggcagtttggccgaaccgg           >  2:545483/5‑233 (MQ=255)
                                                                                                                                                                                                                                               tCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGGAAAATCGAGCAGGCGATGGGGGTAGTCGTAGGTTGGGCCAAGCAGCTGGccgccg  >  1:178595/1‑251 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       
CCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGA‑TATAGCCGCTGCGGATCTCGCCTGCGAACGGG‑TGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAA‑ACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCG  >  NC_000913/4319838‑4320325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑