BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,501	C→T	L371L (CTG→TTG)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,501	0	C	T	99.1%	324.9 / NA	106	L371L (CTG→TTG)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base C (1/0); major base T (50/54); minor base G (0/1); total (51/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 106 total aligned reads displayed.

CGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTA  >  NC_000913/23254‑23744

cGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTgg                                                                                                                                                                                                                                                  <  1:273142/251‑1 (MQ=255)

        ccATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTg                                                                                                                                                                                                                                          >  1:336161/1‑251 (MQ=255)

         cATTCCCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGTCTTCGTCGTTCCGGCAATCCTCGGCGATCACGTTACCCTGTATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCTGACGACTATGTGATCGGTCAGAAATATGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGa                                                                                                                                                                                                                                         <  1:310927/251‑1 (MQ=255)

                             gTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGACGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGAc                                                                                                                                                                                                                     >  1:471574/1‑251 (MQ=255)

                             gTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACACGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGCTGGCGTGAACGTCTTCAAAGCGAACGAc                                                                                                                                                                                                                     >  1:80745/1‑251 (MQ=255)

                                 ggAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACAtcg                                                                                                                                                                                                                 >  1:28330/1‑251 (MQ=255)

                                  gAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACAtcgt                                                                                                                                                                                                                >  1:82804/1‑251 (MQ=255)

                                       tGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGtt                                                                                                                                                                                                            <  1:13146/250‑1 (MQ=255)

                                       tGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGtt                                                                                                                                                                                                            <  1:307621/250‑1 (MQ=255)

                                        gAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACTGTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCTGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTg                                                                                                                                                                                                           <  1:258553/250‑1 (MQ=255)

                                            tgctgcGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCgctg                                                                                                                                                                                                      >  1:196388/1‑251 (MQ=255)

                                             gctgcGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGCAACCGCTAACCCGGTTGGCCCGGACGGCCATTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGGCTTCAAAGCGCACGACATCGTCGTTGCgctgc                                                                                                                                                                                                     >  2:170785/1‑251 (MQ=255)

                                              ctgcGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGCACGACATCGTCGTTGCgctgct                                                                                                                                                                                                    >  1:275912/1‑251 (MQ=255)

                                               tgcGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGctgctg                                                                                                                                                                                                   >  1:384470/1‑251 (MQ=255)

                                                  gcTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCtgctgc                                                                                                                                                                                                  >  1:317134/1‑249 (MQ=255)

                                                   cTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAgg                                                                                                                                                                                               >  1:280203/1‑251 (MQ=255)

                                                       aCCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGaaa                                                                                                                                                                                            >  1:146748/1‑250 (MQ=255)

                                                          cATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAgg                                                                                                                                                                                        >  1:162888/1‑251 (MQ=255)

                                                             ccGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTTCCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGgcg                                                                                                                                                                                      <  1:160317/250‑1 (MQ=255)

                                                             ccGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGgcgc                                                                                                                                                                                     >  1:464541/1‑251 (MQ=255)

                                                                   aTGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCgctgct                                                                                                                                                                               >  1:173906/1‑251 (MQ=255)

                                                                   aTGGGCTTCGACGTCCCGGCAACCCTCGGCAGTCACGTTTCCCGGGATGCCGGTACCTGTGCCGTTCACACCGCGCCTGTCCACGGCCCGGACGACTATGTGAGCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCgctgc                                                                                                                                                                                <  2:549687/250‑1 (MQ=255)

                                                                               gTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGaa                                                                                                                                                                   <  1:444450/251‑1 (MQ=255)

                                                                                 tCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGaaa                                                                                                                                                                  >  1:208410/1‑250 (MQ=255)

                                                                                   cGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGGGAAAATg                                                                                                                                                               >  2:77300/1‑251 (MQ=255)

                                                                                        aTCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATgcagca                                                                                                                                                          <  1:78485/251‑1 (MQ=255)

                                                                                        aTCCTCGGCGAACACGTTACCCTTGATGCCGGTACCGGTTCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTCTGTGATCGGTCAGAAATACGGCCTGGAAAACGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGAACTTATCCGCCGTTGGATGGCGGGAACGTATTCACAGCGAAAGACCTCGTAGTTGCGCTTCTGGCGGAAAAAGGCGCGCGGCTGCACGtttcgaaaaagaccc                                                                                                                                                           >  2:297808/1‑237 (MQ=255)

                                                                                             cGGCTATCCCGTTTCCCTTGATTCCGGTCCCGGTGCCGTTCCCACCGAGCCTGGTAACTGCCCGGCAGACTATGTGAGCTGTCAGAAATACGGCGTGGATCCCGCTTACCCGGTTGGACCTGTCGGCACATATCTGCCGGGCTCTTAAACTACGTTGGATGGAGTGAACGTCTTCAAAGCGAACGACATCGTCGTTTCGCGGCTGCATGAAAAAGGCTCGCTTCCGCACGGTGAGAAAAGGCAGCACAgtt                                                                                                                                                     <  2:552691/251‑3 (MQ=255)

                                                                                                 gATCACGTTAACCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGCCATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGGAAATGCAGCACAGCTATcc                                                                                                                                                 >  1:49700/1‑251 (MQ=255)

                                                                                                 gATCACGGTAACCTGGTTGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGAGCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACATATCTGCCGGGCACTTATCCGTCGTTGGTTGGCGTGAACGTCTTCAAAGCGACCGACCACGTCTGTGTGATGCTCCGGGAAAAAGACGCGCTGCTGCACGTGTAGAAAAGGCAGCCCAGCTATcc                                                                                                                                                 >  2:478933/1‑251 (MQ=255)

                                                                                                   tCACGTTAACCTGGATGCCGGTACCGGTGACGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCACGGACGGCACTTATCTGCCGGGCACTTATCCGCCGTTGGATGGCGTGACCGTCTTCAAAGCGAAAGACATCGTCGTTGCGCTGCTGCCGGAAAAGGGCGCGCTGCAGCACGTTGAGAAAATGCAGCACAGCTaccc                                                                                                                                                 >  2:559525/1‑246 (MQ=255)

                                                                                                   tCACGTAACCCTGGAGGCCGGTACCGGTGCCGTTCACCCCGCGCCTGGCAACGGCCCGGACGACTATGTGCTCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTCTCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCACAGCGACCGCCATCGTCGTTGCGCGACTCCAGGATACAGGCGCGCTGCTGCACGTTGCTCAAATgcagcccatctgtccgg                                                                                                                                               >  2:251011/1‑239 (MQ=255)

                                                                                                       gTTACCCTGGATGCCGGTACCGGTGCCGTTCACCCCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGtgctg                                                                                                                                           <  1:102132/251‑1 (MQ=255)

                                                                                                            ccTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCaaa                                                                                                                                                                                                                              <  1:62080/163‑1 (MQ=255)

                                                                                                             cTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCg                                                                                                                                     >  2:485615/1‑251 (MQ=255)

                                                                                                               ggATGCCGGTACCGGTGCCGTTCACCCCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGATGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTc                                                                                                                                   <  1:566342/251‑1 (MQ=255)

                                                                                                                gATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTca                                                                                                                                  <  1:133890/251‑1 (MQ=255)

                                                                                                                    ccGGTTCCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGTCGACTATGTGATCGGTCAGAAATACGGCCTGGACACCGCTAACCCGGTTGGCTCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTTGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACaaa                                                                                                                              <  1:552387/251‑1 (MQ=255)

                                                                                                                         aCCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGCGAAAATGCAGCACAGCTATCCGGGCTGCTGGCGTCCCAAAACGcc                                                                                                                         >  2:521784/1‑251 (MQ=255)

                                                                                                                                ccGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGatcatc                                                                                                                  >  1:446269/1‑251 (MQ=255)

                                                                                                                                   ttCACACCGCGCCTGGCCACGGCCCGGACGATTAGGTGATGGGCGAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGTACTTATCTGCCGGGAACTTATACGACGTGGGAGGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGatcatc                                                                                                                  <  2:337504/248‑1 (MQ=255)

                                                                                                                                   ttCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTc                                                                                                               >  1:517496/1‑251 (MQ=255)

                                                                                                                                       ccccGCGCCTGGCCTCGGCCCGGACGACTATGGTATCGGTCAGAAATTCGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTcctcg                                                                                                           <  2:30248/249‑4 (MQ=255)

                                                                                                                                        acCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGACACCGCTAACCCGGTTGGCCCGGGCGGCACTTATCTGACGGGCACTTATCCGAAGTTGGATGGCGTGAACGTCTTCACAGCGAACGACATCGTAGTTGCGCTGCTGCCGGAATAAGGCGCGCTGCTGCACGGTGAGAAAATGCAGCACAGCTCTCAGTGCGGCTGGCGACACAAAACGCCGGTCATCATCcgcg                                                                                                           >  2:39313/1‑250 (MQ=255)

                                                                                                                                           gctccTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGAc                                                                                                       <  2:280203/248‑1 (MQ=255)

                                                                                                                                           gcgcCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGAc                                                                                                       <  1:297808/251‑1 (MQ=255)

                                                                                                                                            cgcCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGAcg                                                                                                      <  1:521784/251‑1 (MQ=255)

                                                                                                                                              ccTGGCCCCGGCCCGGACGACTAGGTGATCGGTCAGAAATTCGGCTTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGAcgcc                                                                                                    <  2:23674/251‑1 (MQ=255)

                                                                                                                                              ccTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGAcgcc                                                                                                    <  2:196388/251‑1 (MQ=255)

                                                                                                                                                        gCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTc                                                                                          <  1:464426/251‑1 (MQ=255)

                                                                                                                                                        gCCCGGACGACTATGGGATCGGGCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTAGCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTc                                                                                          <  2:109138/251‑1 (MQ=255)

                                                                                                                                                            ggACGACTATGTGATCGGGCAGAAATACGGTCTGGAACCCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGCCGCCGCAGTGGTTCGTCa                                                                                      <  2:17366/251‑1 (MQ=255)

                                                                                                                                                             gacgacTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTc                                                                                       >  1:248179/1‑249 (MQ=255)

                                                                                                                                                              acgacTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGc                                                                                    <  1:530060/251‑1 (MQ=255)

                                                                                                                                                                gcctatttttgcGGTCCGAAATTCGTCCTGGTACCCGCTAACCCGGGTGGCCCGGTCGGCTCTTACCTGCCGGTGTCTTATCCGACGTTGGATGGAGTGAACGTCTGCAATGCGAACGACATCGACGTTGCGCTTCTGCAGGAAAAAGTCGCGCTCCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTTCTGCTTGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCatcct                                                                                  <  2:415336/240‑5 (MQ=255)

                                                                                                                                                                gacTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCAt                                                                                  <  2:279527/251‑1 (MQ=255)

                                                                                                                                                                gacTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCAt                                                                                  <  1:177361/251‑1 (MQ=255)

                                                                                                                                                                gacTATGTGATCGGTCAGAAATACGGCCTGGAAAACGCTAAACCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTGGGATGGCGTGAACGTCCTCACAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGACGCGAAACTGCAGCCCAGTTATCCGTGCTGCTGGCGTCCCAAACCGCCGATCCTCTTCCGCGCGACTCCGCAgcagttcgcagacat                                                                                  >  2:257960/1‑237 (MQ=255)

                                                                                                                                                                    atgggATCGGTCAGAAATACGGCCTGGAAACCGCAAACCCGGGTGGGCCGGGCGGCACTTATCTGCCGGGCACTGAGCCTACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGGTGCGCTGGTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAAGGCAGCACAGCTATCCGTGCTGCTGTCGTCACAAAACGCCGATGATCTTCCGCGCCAGGCCGCAGTGGGTCGTCAGCATGGa                                                                               <  2:352761/246‑1 (MQ=255)

                                                                                                                                                                    aTGTGATCGGTCCGAAATCCGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGa                                                                               <  2:208410/250‑1 (MQ=255)

                                                                                                                                                                         agcgtacAGACATACGGCCTGTAAACCGCTATCCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACAGCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTAGCCGTGCTGCTGGCGTCACAAAACGCCGCTCATCTGCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGaa                                                                         <  2:85780/245‑1 (MQ=255)

                                                                                                                                                                           cGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGaa                                                                         >  1:288545/1‑249 (MQ=255)

                                                                                                                                                                             gacAGAAATACGGCATGGAAACCGCTAACCCGGTTGGCCCGGACGGCACGTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTAGCCGTGCTGCTGGCGTCACAGAACGCGGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGATAGGt                                                                     <  1:234371/249‑1 (MQ=255)

                                                                                                                                                                             gTCAGAAATACGGACTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCAGGGCACTTAAACGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCATCTGCACGTTGAGAGAGTGCAGCACAGCAATACGGGCTGCTAGCGTCACAAAACTCCGATAATCTTCCGCGCGAAGCCGCAGGGGTTCGTCAGAAGGGAGCCGAAAGGt                                                                     >  2:320928/1‑251 (MQ=255)

                                                                                                                                                                              tCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCCAAGCGAACGACATCGTCGTTGCGCTGCTGCCGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGAGCTGCTGGCAGCACAAAACGCCGATCATAGTCCGCGCGACGCCGCAGCGGGTCGTCCGCAAGGATCCGACAGGTc                                                                    >  2:171128/1‑251 (MQ=255)

                                                                                                                                                                               cAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCGCTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCATTGGTTCGTCAGCATGGATCAGAAAGGTCt                                                                   <  1:171128/251‑1 (MQ=255)

                                                                                                                                                                               cAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACTACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCt                                                                   <  1:43436/251‑1 (MQ=255)

                                                                                                                                                                                   aaTACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGAGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGAAGTGGTTCGTCAGCATGGATCAGAAAGGTCtgcgt                                                               >  1:177315/1‑251 (MQ=255)

                                                                                                                                                                                       cGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTTCGTgcgc                                                           >  2:71582/1‑251 (MQ=255)

                                                                                                                                                                                        ggCCTGGAAACCGCTAACCCGTTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCa                                                          <  1:490054/251‑1 (MQ=255)

                                                                                                                                                                                          catgaaaaCCGCTAACCAGGTTGGCCCGGACGGCTCGAATCAGACGAGCACTTATCCGACGTTGGATGGCGTTAAGGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGAGCGCTGATGCACGTTGAGATAGTGCAGACCAGCTATACGTGCTGCTGGCGTCACCAAACGCAGATCATCTTCCGCGCGACGCCACGGTGGATCGTCAGCATGGAGCAGaacatcctgcgtgcgccgt                                                        >  2:360969/6‑234 (MQ=255)

                                                                                                                                                                                             tgacaacGCTAACACGGGTGGCCCGGACGGCCCTTATCTGCCGGGCACTTATCAGACGCTGGATGGCGTACACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGGGATGCTGGCGTCCCAAAACGCCGATCATCTTCCGCGCGACGCCGCCGTGGTTAGACAGCCTGGATCAGAAAGGTCTGCGTGCGCAGtacc                                                     >  2:537693/7‑248 (MQ=255)

                                                                                                                                                                                             ggAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCAc                                                     >  1:26244/1‑251 (MQ=255)

                                                                                                                                                                                              gAAACCGCTAACCAGGTTGTGCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACAGCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGCCACt                                                    <  2:374571/251‑1 (MQ=255)

                                                                                                                                                                                               aaaCCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTGCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCTACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTg                                                   <  2:263091/251‑1 (MQ=255)

                                                                                                                                                                                                aaCCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTg                                                   >  1:95667/1‑250 (MQ=255)

                                                                                                                                                                                                 accgcttccccGGTTGGCCCCGACGGCTCTTATCTGCCGGGCACTTATTCGCTGTGGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGGTGTTGCGAGGCTGCAGGAAAAAGGCGCGCTGCGGCACGTTGAGATAAGGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAATCGCCGATCATCTTCCGCTCGACGCCGCAGTGGTTCGTCCGCATGGATCAGAAAGGTCTCCGTGCGCAGTCACTGaa                                                 <  2:471574/243‑1 (MQ=255)

                                                                                                                                                                                                   cGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGcaca                                                                                                                                                        <  1:524431/146‑1 (MQ=255)

                                                                                                                                                                                                    gCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGCTGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGCTCAGAAAGGTCTGCGTGCGCAGTCACTGac                                                 >  2:291851/1‑247 (MQ=255)

                                                                                                                                                                                                    gCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAaga                                              <  1:149432/251‑1 (MQ=255)

                                                                                                                                                                                                      tAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAag                                               >  1:348422/1‑248 (MQ=255)

                                                                                                                                                                                                         cccGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATc                                          >  1:519668/1‑250 (MQ=255)

                                                                                                                                                                                                         cccGGTGGGCCCGGTCGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTGCAAAGCGAACGACAGCGTCGGTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCa                                         <  2:544356/251‑1 (MQ=255)

                                                                                                                                                                                                          ccGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGAGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATACAGCACAGCTATCCGTGGTGCTGGCGGCACAAAACGACGATCATCTTCCGCGCAACGCGGCAGCGGTTCGTCAGCATGGATCAGAAAGGTCTGCGGGCGCAGTCACTGAAAGAGCTCa                                         >  2:199575/1‑250 (MQ=255)

                                                                                                                                                                                                           cGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCaaa                                       <  1:485615/251‑1 (MQ=255)

                                                                                                                                                                                                                    cgggcGGCACTTTTCGGCCGGGCACTTATCCGACGTTGGAGGGCGTGAACGTCTTCGAAGCGAACGACATCGTAGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCTCTGAAAGAGATCAAAGGCGTGCAg                              <  2:497069/247‑1 (MQ=255)

                                                                                                                                                                                                                    cGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGc                                >  1:146363/1‑249 (MQ=255)

                                                                                                                                                                                                                     ggACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGt                             >  1:5587/1‑251 (MQ=255)

                                                                                                                                                                                                                        cGGCACTTATCTGCCGGGCACTTCTCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGAGCGCTGCTGAACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACACACCGCCGATCATCTTCCGCGCGACGCCGACGTGGTTCGTCAGCATGGATCAGAAAGGTCAGCGTGCGCAGTCAATGAAAGAGATCAAAGGCGtcacgtgg                           >  2:66728/1‑243 (MQ=255)

                                                                                                                                                                                                                        cGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAACGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGAGTCACAAAACGCCGATCATCTTCCGCGCGCCGCCGCAGTGGTTAGTCAGAATGGATCAGAAAGGTCTGCGTGCGCCGTCACTGAAAGAGATCACAGGCGTGCAGTgg                           >  2:538180/1‑250 (MQ=255)

                                                                                                                                                                                                                        cGGCACTTATCCGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAACGCGATCGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCGTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGa                          <  2:207775/251‑1 (MQ=255)

                                                                                                                                                                                                                            ccTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATccc                      <  1:407064/250‑1 (MQ=255)

                                                                                                                                                                                                                            aCTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCACAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGACAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAACACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATccc                      >  1:133823/1‑251 (MQ=255)

                                                                                                                                                                                                                            aCTTATCTGCCGGGCACTTAGCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATccc                      <  1:321470/251‑1 (MQ=255)

                                                                                                                                                                                                                               tATCTGCCGGGCACTTATCCGACGGTGGAAGGCGTGAACGTCTTCAAAGCGATCGACAGCGTCGTTGCGCTGCGGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCGGCACAGCTATCCGGGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCAGGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTTCATCCCgg                    <  2:331576/250‑1 (MQ=255)

                                                                                                                                                                                                                                    gCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTGCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTg                <  2:5587/249‑1 (MQ=255)

                                                                                                                                                                                                                                       gggCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACAGCGTCGTTGCGCTGCGGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGcc           <  1:22366/251‑1 (MQ=255)

                                                                                                                                                                                                                                          cACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCAAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATAAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAgg        >  1:296651/1‑251 (MQ=255)

                                                                                                                                                                                                                                             ttATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGgcgc     >  1:560387/1‑251 (MQ=255)

                                                                                                                                                                                                                                                 ccGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTa  <  1:324399/250‑1 (MQ=255)

CGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTA  >  NC_000913/23254‑23744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`