Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,282,536	C→T	Q180Q (CAG→CAA)	yjcF ←	pentapeptide repeat‑containing protein YjcF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,282,536	0	C	T	100.0%	78.2 / NA	25	Q180Q (CAG→CAA)	yjcF	pentapeptide repeat‑containing protein YjcF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/16);  total (9/16)

CTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACCATTTACTGTCTGTGGGATTTCGATAGTATTATTATGGACGGTGACATTATTTATAAAATATACCACCTTAATTGACACTTCCTTATCTTTATTAAGGTAAACTCTTCTCGTAATTCTATCGTCACATTTATT  >  NC_000913/4282292‑4282775                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                cTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGaaa                                                                                                                                                                                                                                            >  1:462799/1‑250 (MQ=255)   ttATAAAGATTTACCTCAGCCATGTATGCCATATAAAAATTTGAATAATCAAGTAAGGGATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAAGTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGaaaa                                                                                                                                                                                                                                           <  1:516866/249‑1 (MQ=255)      aaaaGATTTACCTCAGCCATGTATGCCATATAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTTAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGc                                                                                                                                                                                                                                      <  2:537846/250‑1 (MQ=255)                          gTATGCCATATAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCt                                                                                                                                                                                                                   <  1:112291/250‑1 (MQ=255)                                   ataAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATTGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCGAAATTCg                                                                                                                                                                                                         >  1:182696/1‑251 (MQ=255)                                                       ggaaggTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCt                                                                                                                                                                                     <  1:24727/251‑1 (MQ=255)                                                                     atatTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATACATAGCGGCACATGATTTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTTAACAATCGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTGCATCTTTAATCACGAAAcc                                                                                                                                                                       >  2:197170/1‑251 (MQ=255)                                                                       atTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCtt                                                                                                                                                                     <  1:273904/251‑1 (MQ=255)                                                                                         tgCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGaa                                                                                                                                                    <  1:197170/250‑1 (MQ=255)                                                                                                        aaCATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACCAt                                                                                                                                    >  1:143635/1‑251 (MQ=255)                                                                                                         aCATGCATAGCGGAACATGAATTTAGAGTTCCTTCAGTCAGTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACCAtt                                                                                                                                   <  2:122492/251‑1 (MQ=255)                                                                                                                                       ccGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAACTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACCATTTACTGTCTGTGGGATTTCGATAGtattat                                                                                                      <  1:384653/250‑1 (MQ=255)                                                                                                                                       ccGTCAATCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATGATCAAAATGTATTTTTTCCATTATGGCGAAAGTAAAATTCGGTTTATACATATTACATTTTTGAAAAATAGCGTTTTGTAGTCTGTTAACTGCAAAATTCTAATTGGATAAATCTTCATCTTTCATCACTATTCCTTTCAGGCCAAGGTGTTAAAAATCTTACCCACCATTTTCTGTCTGTGGGATCTCGACAGtaggatt                                                                                                     <  2:23053/251‑6 (MQ=255)                                                                                                                                           cagtcaTTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACCATTTACTGTCTGTGGGATTTCGATAGTATTATTATgg                                                                                                 >  1:401582/1‑251 (MQ=255)                                                                                                                                                   tttATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACCATTTACTGTCTGTGGGATTTCGATAGTATTATTATGGACGGTGa                                                                                          <  1:534610/250‑1 (MQ=255)                                                                                                                                                                 aTTTGAGTCAACGAGAATACAATTATCAAAAAGTATTTTTTCCATTATTGCGAAATTAAAATTCGTTTTATACATATTACAGGCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCGAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACCATTTACTGTCTGTGGGATTTCGATAGTATTATTATGGACGGTGACATTATTTATAAAat                                                                           <  2:182696/251‑1 (MQ=255)                                                                                                                                                                            cGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACCATTTACTGTCTGTGGGATTTCGATAGTATTATTATGGACGGTGACATTATTTATAAAATATACCACCTTa                                                                <  2:462799/251‑1 (MQ=255)                                                                                                                                                                              agaATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACCATTTACTGTCTGTGGGATTTCGATAGTATTATTATGGACGGTGACATTATTTATAAAATATACCACCTTAAt                                                              >  1:183934/1‑251 (MQ=255)                                                                                                                                                                                   aCAATTATCAAAAAGTATTGTTGCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATATCGTTTTGTAGTATGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACCATTTTCTGTCTGTGGTATTTCGATAGTATTATTATGGACGGTGACATTATTTATAAAATATACCACCTTAATTGac                                                          <  2:492746/250‑1 (MQ=255)                                                                                                                                                                                           aaaaaaGTATTTTTTCCATTATGGCTAAATTAAAATTCGTTTTATACATATTAAAGTCTTGAAAAATAGCGGTTTGGTGTCTGCAACCTGCAAAAGTCGAAGTGGATAAATCTTCATCTTTAATTACGATACCTTTTAGGCTAAGGTGTGAAAAATCGTAACCACCATTTACTGTCTTTGGTATTTCGATAGTATTAGTATGGACGTTGACATTAGTTATAAAATATTCCCCCTTAATTGACACTTCCTTa                                                 <  2:559760/250‑1 (MQ=255)                                                                                                                                                                                                    ttttttCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGACAAATCGTAACCACCATTTACTGTCTGTGGGATTTCGATAGTATTATTATGGACGGTGACATTATTTATAAAATATACCACCTTAATTGACACTTCCTTATCTttatt                                         >  1:167825/1‑250 (MQ=255)                                                                                                                                                                                                                tGGCGAAAGTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGAt                                                                                                                                                                                                 <  1:512427/86‑1 (MQ=255)                                                                                                                                                                                                                 ggCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACCATTTACTGTCTGTGGGATTTCGATAGTATTATTATGGACGGTGACATTATTTATAAAATATACCACCTTAATTGACACTTCCTTATCTTTATTAAGGTAAACtcttc                           >  1:425174/1‑251 (MQ=255)                                                                                                                                                                                                                                   tttATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACCATTTACTGTCTGTGGGATTTCGATAGTATTATTATGGACGGTGACATTATTTATAAAATATACCACCTTAATTGACACTTCCTTATCTTTATTAAGGTAAACTCTTCTCGTAATTCTATCGTca          <  1:334163/250‑1 (MQ=255)                                                                                                                                                                                                                                          atatTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACCATTTACTGTCTGTGGGATTTCGATAGTATTATTATGGACGGTGACATTATTTATAAAATATACCACCTTAATTGACACTTCCTTATCTTTATTAAGGTAAACTCTTCTCGTAATTCTATCGTCACATttatt  >  1:387270/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                CTTTATAAAGATTTACCTCAGCCATGTATGCCATATAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACCATTTACTGTCTGTGGGATTTCGATAGTATTATTATGGACGGTGACATTATTTATAAAATATACCACCTTAATTGACACTTCCTTATCTTTATTAAGGTAAACTCTTCTCGTAATTCTATCGTCACATTTATT  >  NC_000913/4282292‑4282775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑