Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,219,752 C→T S386F (TCC→TTC)  aceK → isocitrate dehydrogenase kinase/phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,219,7520CT94.1% 94.5 / NA 34S386F (TCC→TTC) aceKisocitrate dehydrogenase kinase/phosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (18/14);  minor base G (0/2);  total (18/16)
Fisher's exact test for biased strand distribution p-value = 2.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCACCCG  >  NC_000913/4219508‑4219995
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     
cTACAGAGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAGTGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGGATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAATGAGATGTCTGCCGCCCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCTGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATTTTTTCCCGGc                                                                                                                                                                                                                                                 >  2:367143/1‑251 (MQ=255)
    aGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTa                                                                                                                                                                                                                                             <  1:449158/251‑1 (MQ=255)
     gggCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGGGGAATGGGTATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGGTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCTCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAACCTTTGTGCTGGAGAAGCGGCATATTTTCCCGTCATTaa                                                                                                                                                                                                                                            <  2:513127/251‑1 (MQ=255)
          gTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAACGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCCTATTTTCCCGGcc                                                                                                                                                                                                                                                >  2:281600/1‑241 (MQ=255)
          gTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCa                                                                                                                                                                                                                                                <  1:281600/242‑1 (MQ=255)
                           gAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTGACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAagc                                                                                                                                                                                                                      <  1:103668/251‑1 (MQ=255)
                                   gggTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGaa                                                                                                                                                                                                               >  1:301659/1‑250 (MQ=255)
                                      tATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGaaaaa                                                                                                                                                                                                            >  1:499443/1‑250 (MQ=255)
                                           gTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCAcc                                                                                                                                                                                                      >  1:121620/1‑251 (MQ=255)
                                                                 ttagctgcgcggttttGATCGGTTTTTAAATGTCATCAAAGACAGGGTCTCGCCTCAAAAATACATTCCTGCCGCTCAAGTTCGTTCCAGCTATCAACTGGTGAAAGAGCACGATCGCTTGGGCCGAATTGCGGACTCCCCGGAGTTTGAAAACTTTGTGCTGGAGAAGCTGCATATTTTCCCGTCATTAATTGAATTACTGCTTCAGGAAGCAGCGGTAAAAATCACCGATCTCGGCGAACAAATtgtg                                                                                                                                                                                 <  2:304813/237‑1 (MQ=255)
                                                                   cGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGGGAtt                                                                                                                                                                              >  1:179666/1‑251 (MQ=255)
                                                                                               aGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATgg                                                                                                                                                  >  1:270773/1‑251 (MQ=255)
                                                                                               aGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATgg                                                                                                                                                  >  1:406366/1‑251 (MQ=255)
                                                                                                  catcaAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGc                                                                                                                                               >  1:559111/1‑251 (MQ=255)
                                                                                                  catcaAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGGTCGGGCCTGCTATCAACGGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGGGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTg                                                                                                                                                <  2:557947/250‑1 (MQ=255)
                                                                                                       aaGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTc                                                                                                                                          <  1:554370/251‑1 (MQ=255)
                                                                                                               ttCGCGCCGCAGAAAGAGATGTCTGCAGCTCTCGTTCGTGCCTGCTATCAACTGGTAAAAGAGCACGATCGCGTGGGCCGAATGGCGGACAAACAGTAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCGTTAATAGAATTACTGCTTCAGGACGCAGCGGAGAACATGCCCGATCTCGACGAACAAATTGTGAATAGCCATATTTATATTGAgcggagtaaggtgcagcttaagatctg                                                                                                                                  >  2:334786/1‑228 (MQ=255)
                                                                                                                tcgagcctcAGAAAGAGATGCCTGCCGCTCTCGTTCGTGCCTGCTATCAACTGGGTAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACGTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATctg                                                                                                                                  <  2:312179/242‑1 (MQ=255)
                                                                                                                tCGCGCCGCAGAAAGAGCTGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGCACAAATTGAGATTCGCCATATTTATATTTAGCGGCGGGTAGTGCCGCtacaaatctgg                                                                                                                                 >  2:246738/1‑241 (MQ=255)
                                                                                                                 cgcgCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATctggc                                                                                                                                <  1:206/251‑1 (MQ=255)
                                                                                                                      cgcAGAAAGAGAAGTACGCCGCTCTCGGTCGTGCCTGCGATCAACTTGTGAAAGAGCACGAGCGCGTGGTCTGAATGGCGGACTCCCAGGAGTTAGAAAACTTTGTGCTGGAGAAGCGGCATATTTGCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGACCTCGGCGAACAAATTGTGATTAGCCATCTTTATATTGATCGGCGGATTGTGCCGCTCAATATCGGGCTGGaa                                                                                                                           <  2:147752/251‑1 (MQ=255)
                                                                                                                             agagATGTCTGCCGCTCACGGTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACACAGGAGTTTGAAAACGTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCTGCTCAATATCGGGCTGGAACAAGTgg                                                                                                                    <  1:103621/251‑1 (MQ=255)
                                                                                                                                                                   tGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGGGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGCCGCCATTGAAGAATaatgt                                                                             >  1:313881/1‑247 (MQ=255)
                                                                                                                                                                          agagCACGATCGCGTGGGCCGAATGGCGGCCACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCCTATTTTCCCGGCATTAATGGAATTACTGCTTCAGGACGCAGCGGAAAAAATCACCGCTCTCGGCGAACAAATTGTGATTCGCAATCTTTATCTTGAGCGGCGGATGGTGCCGCTAAATATCAGGCTGGATAAAGTGGAAGGTTCGCAGATGGGTGACGCCATTgacacatacggtaccgcc                                                                       >  2:274409/1‑234 (MQ=255)
                                                                                                                                                                           gagCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAGGGTCAGCAGTTGCGCGACGCCCTTGAAGAATACGGTAACGCTAt                                                                     >  1:492082/1‑251 (MQ=255)
                                                                                                                                                                                      cgggggtctaaTGGCGGACACCCAGGATTTGGAAAACTTTGTGCTGGAGAAGCGGCATATTTGCCCGGCATTAACCGCATTGCTGCTTCAGGAAGCATCGGAAAAAATCACCGATCACGGCGAACAAATTGTGATGCGCCGTCTTGATATTGAGCGGCGGATGGTGCCGCTCAATATCTTGCTGGAATAAGTGGTAGTGTAGCAGTTGCGAGACGCCATTGAAGAATTCGTTAACGCTATTCGCCTGCTTg                                                          <  2:553442/242‑1 (MQ=255)
                                                                                                                                                                                                 tgacGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGATGCGGCATATTTTCCCGGCATTAATGGACTTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGCGCGGCGGCAGGTGCCGCTTATTATCTAGCTGGACCCAGTGGTAGGACTGCCTATGCGCGCCGCCATTGAGAAATACGGTAACGCGATTCTCCAgtgttccacggctacc                                               >  2:164045/4‑236 (MQ=255)
                                                                                                                                                                                                   gCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCAGATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACAt                                             <  2:216577/251‑1 (MQ=255)
                                                                                                                                                                                                             aGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGc                                   <  1:520021/251‑1 (MQ=255)
                                                                                                                                                                                                                   ttGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACAt                              <  1:542391/250‑1 (MQ=255)
                                                                                                                                                                                                                      aaatgtttGTGCTGGAGAAGCGGCCTATTGTCCCGGTATTAATGGAATTACTGCTTCAGGAAGCCGCGGAAAAAATCACCGATGTAGGCGAACAAATGGTGAGTCGCCATCTTTATATTTAGCGGCGGATGGTTCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGTCGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTATCATTTTCCCTGTCGACATGCTg                          <  2:538022/246‑1 (MQ=255)
                                                                                                                                                                                                                                gCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGAGGAAGGGCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTGGCCGCTGCTAACATTTTCCCTGGCGACATGCTTTTTAAAAACt               >  2:172052/1‑251 (MQ=255)
                                                                                                                                                                                                                                    gagaAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAACATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACCTGCTGTTTAAAAACTTCgg           >  1:324465/1‑251 (MQ=255)
                                                                                                                                                                                                                                             cATATTTTCTCGGAATTAATGGAATTAATGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATTTCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGATAGCCGCTGCTAACATTTTCCATGGCGAAATGCTGATTTACAAATTCGGTGTCAACCg  >  2:293330/1‑251 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     
CTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCACCCG  >  NC_000913/4219508‑4219995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: