Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,178,883 C→T A2V (GCT→GTT)  rplA → 50S ribosomal subunit protein L1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,178,8830CT97.1% 200.7 / NA 70A2V (GCT→GTT) rplA50S ribosomal subunit protein L1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (33/35);  minor base G (1/1);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGTTCTTTCACTTTCGTTACCAAGACCCCGCCGGCAGCAGTTCTGCTGAAAAAAGCGGCTGGTATCAAGTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGCGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAAC  >  NC_000913/4178638‑4179127
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     
cagTTCTTTCACTTTCTTTGCCAAGACCCCGCCGGCAGCAGTTCTGCTGAAAAAAGCGGCTGGTATCAAGTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAAc                                                                                                                                                                                                                                                  <  2:492568/249‑1 (MQ=255)
 cGTTCTTTCACTTTCGTTACCAAAACCCCGCCGGCAGCAGTTCTGCTGAAAAAAGCGGCTGGTATCAAGTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGACGCGATGACTCGCTCCATCGACGGGAATGCACGTTCCATGGGCATGGGAGTGGAGGACTAAGAAATAGGTAAACt                                                                                                                                                                                                                                                 >  2:404117/1‑251 (MQ=255)
      ttcctcTTTCGTTACCAAAACCCCGCAGGCAGCAGTTCTGCTGAAAAAAGCGGCGGGTATCAAGTCTGGTGCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACGCGCTCCATCGAAGGTACTGCACGTTCCATGGTCCTGGTAGTGGAGGACTAATAAATGGTTAAACGGAcc                                                                                                                                                                                                                                             <  2:372110/245‑1 (MQ=255)
         acctttctttACCAAGACCCCGCCGGCAGCAGTTCTGCTGAAAAAAGCGGCTGTGATCAAGTCTGGGTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCTCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTTGTTCCGACATTGAAGCGATGACGCGCTCCATCGAAGGTACTGCACGGTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTACACGGACCAAg‑c                                                                                                                                                                                                                                        <  2:318773/249‑1 (MQ=255)
           cTTTCGTTACCAAGACCCCGCCGGCAGCAGTTCTGCTGAAAAAAGCGGCTGGTATCAAGTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAg‑cgc                                                                                                                                                                                                                                      >  1:495249/1‑251 (MQ=255)
             ttCGTTCCCAAGCCCCCGCCGGCAGCAGTTCTGCTGAAAAAAGCGGCTGGTATCAAGTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCa                                                                                                                                                                                                                                     <  1:27998/250‑1 (MQ=255)
                gTTACCAAGACCCCGCCGGCAGCAGTTCTGCTGACAAAAGCGGCTGGTATCAAGTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCg                                                                                                                                                                                                                                 >  1:150127/1‑251 (MQ=255)
                gTTACCAAGACCCCGCCGGCAGCAGTTCTGCTGAAAAAAGCGGCTGGTATCAAGTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGGAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCg                                                                                                                                                                                                                                 >  1:44261/1‑251 (MQ=255)
                   aCCAAGACCCCGCCGGCAGCAGTTCTGCTGAAAAAAGCGGCTGGTATCAAGTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCgtgt                                                                                                                                                                                                                              >  1:29839/1‑251 (MQ=255)
                     aaaGACCCCGCCGGCCTCAGGTATGCTGAAAAAAGCGGCTGGGAACAAGTCTGGTGCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTa                                                                                                                                                                                                                            <  2:23508/250‑1 (MQ=255)
                        gACCCCGCCGGCAGCAGTTCTGCTGAAAAAAGCGGCTGGTATCAAGTCTGGTTCCGGTAAGCCGAACAAAGCCAAAGTGGGTAAAATTTCCCGCGCTCAGCTGAAGGAAATCGCGCAGACAAAAGATGCCGCCATGACTGGGGCCGACATTGAAGCGATGCGTCGCTCCATCGCAGGGACTGCCAGTTCCATGGGCCTGGTAGTGGAGGGCTACGAAATGGTTAAAATGTCCAAG‑CGCAGGCGGGTTATcc                                                                                                                                                                                                                         >  2:510089/1‑251 (MQ=255)
                             cgccgGAAGCAGTTCTGCTGAAAAAAGCGGCTGGTATCAAGTCTGGTTCCGGTAAGACGAACAAAGCCAACGAGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAATGCTGCCGACATGACTGGGGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGAAGTGGAGGACTAGTCAATGGTTAAACTGACCAAG‑CGCATGCGGGTTATCcgag                                                                                                                                                                                                                      >  2:269956/1‑247 (MQ=255)
                              gccgGCAGCATTTCTGCTGAAAAAAGCGGCCGGTATCAAGTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATCGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCgaga                                                                                                                                                                                                                   <  1:397850/251‑1 (MQ=255)
                                 ggCAGCCGTTCTGCTGAAAAAAGCGGCTGGTATCAAGTCTGGTTACGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTGCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCCGCCGACATGACTGGTGCCGACATTTAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCGGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAg                                                                                                                                                                                                                <  2:443562/251‑1 (MQ=255)
                                  gcagcaGTTCTGCTGAAAAAAGCGGCTGGTATCAAGTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGGTATCCGCGAGAAAGt                                                                                                                                                                                                               >  2:224336/1‑251 (MQ=255)
                                    agcagTTCTGCTGAAAAAAGCGGCTGGTATCAAGTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTg                                                                                                                                                                                                             >  1:129302/1‑251 (MQ=255)
                                    agcagTTCTGCTGAAAAAAGCGGCTGGTATCAAGTCTGGTTCCGGTAAGCCGAACAAAGAAAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACAGACCCAG‑CGAATGCGAGTTGTCCGCGAGAAAgatg                                                                                                                                                                                                             >  2:20014/1‑248 (MQ=255)
                                     gcagTTCTGCTGAAAAAAGCGGCTGGTATCAAGTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGa                                                                                                                                                                                                            <  1:326595/251‑1 (MQ=255)
                                             gctgAAAAAAGCGGCTGGTATCAAGTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCaa                                                                                                                                                                                                       <  1:204031/248‑1 (MQ=255)
                                             gctgAAAAAAGCGGCTGGTATCAAGTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAAcc                                                                                                                                                                                                     <  1:300265/250‑1 (MQ=255)
                                                              gaaTCAAGTCTGGTTCCGGTAAGCCGAACAAAGAAAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCAGACATGACTGAGGCAGACATTGAAGCGATGACTCGCTCCATCGACGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGCACTACGAAATGGTTAAACTCACCCAGACCCA‑GCGTGTTAACGGCGAGACAGTTGCAGCAACCAACCATTAAGTCATCAAc                                                                                                                                                                                   >  2:73959/3‑251 (MQ=255)
                                                                  cAAGTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGGTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAg                                                                                                                                                                               <  2:149867/251‑1 (MQ=255)
                                                                    aGTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCTCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGCGGACTAAGAAATGGTTAAACTGACAAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCt                                                                                                                                                                             >  2:269220/1‑251 (MQ=255)
                                                                         ggTTCCGGTAAGCCGAACAAAGACAAAGGGGGTAAAATTTCCCGCGCTCAGCTGCAGGACATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCACCCAAACAGTACGACATCATCGAAGCTATCg                                                                                                                                                                         <  2:276928/250‑1 (MQ=255)
                                                                         ggTTCCGGCAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCACAGCTGCAGCAAATCGCGCAGACCAAACCTGCCGACAGGACTTGTGCCGACATTGAAGCGATGCCTCGCTCCATCGAAGTTACTACACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTTACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTAGATGCAACCAAACATTACGACGTCATCGAAGCTa                                                                                                                                                                            <  2:212130/247‑1 (MQ=255)
                                                                               ggTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCActgct                                                                                                                                                                  >  1:6852/1‑251 (MQ=255)
                                                                                     ccGAACAAAGACAAAGTGTGTAAAAAGTCCCGCGCTCAGCTGCAGGAAACCGCGCAGTCAAAAGCGGCCGACATGACGTGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTTCTGCACGTTCCATGGGCCTGGTAGTGGAGGACGAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCTCACTGCTGAAaga                                                                                                                                                            <  2:434444/251‑1 (MQ=255)
                                                                                     ccGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGaa                                                                                                                                                               >  1:561614/1‑248 (MQ=255)
                                                                                       gAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGCGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGc                                                                                                                                                          >  1:235186/1‑251 (MQ=255)
                                                                                        aaCAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGAATAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGATATCTCACTGCTGAAAAAGcg                                                                                                                                                         >  2:231305/1‑250 (MQ=255)
                                                                                                caaagTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAACTCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAAAGAAGCTGTCGCACTGCTGAAAGAGCTGGCGACTg                                                                                                                                                 >  2:382674/1‑251 (MQ=255)
                                                                                                 aaagTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGc                                                                                                                                                <  1:510089/251‑1 (MQ=255)
                                                                                                      gggTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTaaa                                                                                                                                            <  1:20014/250‑1 (MQ=255)
                                                                                                      gggTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGAACTGCACGTTCCATGGGCATGGAAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGATGATGCAACCAAACAGAACGACATCAACGAAGCTATCAACCTGTTGAAAGAGCAGGCGACTGCTAAAt                                                                                                                                           >  2:542721/1‑251 (MQ=255)
                                                                                                       ggTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAAtt                                                                                                                                          >  1:383594/1‑251 (MQ=255)
                                                                                                        gTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTaaagtc                                                                                                                                         <  1:316942/251‑4 (MQ=255)
                                                                                                        gTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTc                                                                                                                                         <  1:316951/251‑1 (MQ=255)
                                                                                                            aaTTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGt                                                                                                                                       >  1:350967/1‑249 (MQ=255)
                                                                                                                tCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGCTGACTCGCTCCATCGACGCAACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGAATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAACCAGTACGACATCCAAGAAACTATCGCACTGCTGAAAGAGCTGGCGACTGCTCAATTCGTAGaa                                                                                                                                   >  1:540540/1‑249 (MQ=255)
                                                                                                                 cccGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAgt                                                                                                                                <  1:490833/251‑2 (MQ=255)
                                                                                                                         cAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGAc                                                                                                                          <  2:5195/249‑1 (MQ=255)
                                                                                                                                       cgcgCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGCTGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTCCGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCTTAGAAAGTGTGGACGTAGCTGTTAACCTCg                                                                                                          <  2:44261/251‑1 (MQ=255)
                                                                                                                                            aGACCAAAGATTCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCGCCATCGAAGGTACGGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAATTTGATGCAACCAAACAGTACTACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATc                                                                                                     <  2:510625/251‑1 (MQ=255)
                                                                                                                                                 aaaGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGc                                                                                                >  2:219799/1‑251 (MQ=255)
                                                                                                                                                         cggtatgtccgagtctgACATTGAAGCGAGGCCCCGCTCCATCGAAGGTACTGCACGGTCCCTGGGCTTGGTAGTGTATGACTTAGAAATGGTTAAACTGAACAAG‑CGCAATCTTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTAGCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTTGACGTAGCTGTTACCCGCGTCATCGACGCTCGTAAAt                                                                                        <  2:534573/235‑1 (MQ=255)
                                                                                                                                                              ggACTGGTGCCGAAAGTGAAGTGATGACTCGATCCATCGAAGGTTATGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACGGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGAc                                                                                   <  2:141102/250‑1 (MQ=255)
                                                                                                                                                               gACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGAcc                                                                                  <  1:218423/251‑1 (MQ=255)
                                                                                                                                                                   ggTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATAGGCCTGGTAGTGGCGGACTAAGAAATGGTTAAACTGACCACG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGAGTGGACGTAGCTGTTAACCTCGGCATCGACGCTAGTAAATCTgcccgga                                                                               >  2:422132/1‑244 (MQ=255)
                                                                                                                                                                    gTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAac                                                                             >  1:362865/1‑251 (MQ=255)
                                                                                                                                                                      gCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAacgt                                                                           <  1:488089/251‑1 (MQ=255)
                                                                                                                                                                         gACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCGCGGCATCGACGCTCGTAAATCTGACCAGAacgtacg                                                                        <  2:181151/251‑1 (MQ=255)
                                                                                                                                                                               gAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGc                                                                  <  1:33975/251‑1 (MQ=255)
                                                                                                                                                                                       gACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTAc                                                          <  1:174842/251‑1 (MQ=255)
                                                                                                                                                                                       gACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTAc                                                          >  1:464663/1‑251 (MQ=255)
                                                                                                                                                                                            gCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCg                                                     >  1:486976/1‑251 (MQ=255)
                                                                                                                                                                                             cTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGc                                                    >  1:519610/1‑251 (MQ=255)
                                                                                                                                                                                               ccATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCAc                                                  >  2:546838/1‑251 (MQ=255)
                                                                                                                                                                                               ccATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCAc                                                  >  1:95650/1‑251 (MQ=255)
                                                                                                                                                                                                    gAAGGTACTGCCCTTTCCATGGGCCTGGTAGTGGAGGACAAAGAAATGTTTAAACTGACCAAG‑CGCATGCGTTTTATCCGCGAGAAAGTTTAAGCAACCAAACAGCACGACAGCAACGAAGCTATCGCACTTTTGAAGGATCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGGAGCGGTTAACCTCGGCATCGACGCTCGTAAATCTGACTATAACGAACGTGGTGCAACTGTACTGCCGCACGGTAc                                             <  2:235186/251‑1 (MQ=255)
                                                                                                                                                                                                                     cATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTcgcg                            <  2:495249/251‑1 (MQ=255)
                                                                                                                                                                                                                        gggCCTAGTAGTGGTGGCATAAGAAATGGTTAAACTGACCAAG‑CGCCTGCGTGTTATCCGCGAGAAAGTTGATGAAACCAAACAGTACGCCATCAACGACGCAATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGGAGAGAGTGTGGACGTAGCTGTAAACCCAGACATCGACGATCGTAAATCTGCCCAGAACGTACATGGTACAACTGTACTACCCCACGGTAATGGccgtacccctcgcgt                           >  2:235746/1‑238 (MQ=255)
                                                                                                                                                                                                                            cTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAAAGTACGTGGTGCACCTGTACTGCCGCACGGTACTGGACGTTCCGTTCGCGTAGCCGt                     >  2:526005/1‑251 (MQ=255)
                                                                                                                                                                                                                            cTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAAAGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACATCGGCATCGACGCTCGTCACTCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCgg                     >  2:542333/1‑250 (MQ=255)
                                                                                                                                                                                                                               gTAGTGGAGGAATAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGCCATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTCCGTGGTGCAACTGAACTGCAGCACGGTACTGGCCGTTCCATTAGAGTAGCCGtctt                  >  2:409157/1‑248 (MQ=255)
                                                                                                                                                                                                                                 aGTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAttt                 <  1:224336/250‑1 (MQ=255)
                                                                                                                                                                                                                                  gTGGAGGACTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTAc               <  1:39611/251‑1 (MQ=255)
                                                                                                                                                                                                                                     gaggaCTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCa            <  1:429465/251‑1 (MQ=255)
                                                                                                                                                                                                                                        gaCTAAGAAATGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACAACAACGAAGCTATCGCACTGCGGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAgg         <  2:362865/251‑1 (MQ=255)
                                                                                                                                                                                                                                               aaaTGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTTATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATGCGTAGAAAGTGTGGACGTAGCTGTTAACCGCGGCATCGACGCTCGTAAATCTGACCAGACCGTACGTGGTGCAACTGTACTGCAGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAAc  <  2:150127/251‑1 (MQ=255)
                                                                                                                                                                                                                                               aaaTGGTTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAAc  >  1:349024/1‑251 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     
CCGTTCTTTCACTTTCGTTACCAAGACCCCGCCGGCAGCAGTTCTGCTGAAAAAAGCGGCTGGTATCAAGTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAG‑CGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGCGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAAC  >  NC_000913/4178638‑4179127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑