Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,937,997	G→A	G235D (GGT→GAT)	rbsK →	ribokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,937,997	0	G	A	100.0%	222.4 / NA	68	G235D (GGT→GAT)	rbsK	ribokinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/33);  total (35/33)

AATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAGCGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGGTGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAA  >  NC_000913/3937755‑3938240                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    aaTCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGCCGAAATGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAACAGAAAAGCTCACCGGTAATCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGAGCAGGTACTGCATGGAAAAGGTATCCGTACTGTAATTCTTACTTTAGGAAGTCGTGGTGGATGGGGTAGCGTGAATAATGAAGGtt                                                                                                                                                                                                                                             >  2:224918/1‑250 (MQ=255) aaTCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTc                                                                                                                                                                                                                                             <  1:303335/251‑1 (MQ=255)      cccATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAgcgc                                                                                                                                                                                                                                        >  1:438117/1‑251 (MQ=255)       ccATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACTAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGcgcg                                                                                                                                                                                                                                       <  1:139447/251‑1 (MQ=255)       ccATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGcgcg                                                                                                                                                                                                                                       >  1:304973/1‑251 (MQ=255)           cAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTcc                                                                                                                                                                                                                                   <  1:355521/251‑1 (MQ=255)                    aCTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCg                                                                                                                                                                                                                          <  1:60390/251‑1 (MQ=255)                          ggtGCGCTTACCCCGGCTCCGGCTCGCGAAATTCCTTAAGACCTGCTGGCGCTGGTGGCCATTATTAAGCCCAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGGTGCAAAGAAGGCGGCGCAGGTACTGCATGAAAAAGGGATCCGTAATGTACTGATTGTTCTAGGAGGTCGTGGGGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCAGGATTCCgggcga                                                                                                                                                                                                                     >  2:526301/3‑247 (MQ=255)                          gTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATAATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGGCGTGGTGAATGGGATAGCGTGGATTATGAAGGTCAGCGCGTTCCTGGATTCCgggggcg                                                                                                                                                                                                                    >  2:311350/1‑247 (MQ=255)                            tgctctgaaCCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAg                                                                                                                                                                                                                   <  2:521427/243‑1 (MQ=255)                               gcTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTg                                                                                                                                                                                                               >  1:160003/1‑251 (MQ=255)                                gtttaCCCGGCGCCGGCTCGCGAACGTCCTGACGAACTGCTGGCGCTGGTGGACAATATTACGCCAAACGAAACGGAAGCAGAAAAGCGCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGGACTGATTACTTTAGGAAGTCGTGGTGTAGGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCt                                                                                                                                                                                                                <  2:183251/245‑1 (MQ=255)                                  tAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGTTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCAGGAAAAAGGAATCCGTACTGTACTGGTTACTTTAGGAAGTCGTGGAGGATGGGCTAGAGTGAATGATGGAGGTCAGAGCGTTCCTGGATTCCGGGGGCAGGCt                                                                                                                                                                                                                >  2:440601/1‑247 (MQ=255)                                  tAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCt                                                                                                                                                                                                                <  1:440601/247‑1 (MQ=255)                                   aaCCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCg                                                                                                                                                                                                            <  1:224918/250‑1 (MQ=255)                                      ccggctccggctCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATAc                                                                                                                                                                                                        <  1:458128/251‑1 (MQ=255)                                            ccggctcGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATtgc                                                                                                                                                                                                  >  1:568144/1‑251 (MQ=255)                                             cggagctcgaacatacTGACGAACTGCGTGCGTTGTTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGGGTTGAAAAGGATGAAGATTCAACGATGTCGTCGCAGGTACTGCATGAAAAAGGTATCCGTTCAGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTTAAGGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTCTTGATACCATtgct                                                                                                                                                                                                 <  2:421289/236‑1 (MQ=255)                                                  cgcgAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCg                                                                                                                                                                                             <  2:134928/250‑1 (MQ=255)                                                           ccTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCt                                                                                                                                                                                    <  1:456662/250‑1 (MQ=255)                                                            cTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGACGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCttt                                                                                                                                                                                  >  1:452129/1‑251 (MQ=255)                                                               gcGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAAc                                                                                                                                                                               <  2:150827/250‑1 (MQ=255)                                                                 gAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACgg                                                                                                                                                                             >  1:435863/1‑251 (MQ=255)                                                                 gAACTGCTGGCGCTGGTGAACATTATTACGCAAAACGCAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACgg                                                                                                                                                                             >  1:159024/1‑251 (MQ=255)                                                                                gtggACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGc                                                                                                                                                              >  1:164596/1‑251 (MQ=255)                                                                                    aCATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTg                                                                                                                                                          >  1:197942/1‑251 (MQ=255)                                                                                         aataCGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGCAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGGTTCCGGGTGCAGGCTGTCGATACCAGTGCTGCCGGAGTTGCCTTTACCGGTGCGATAATCACGGACTTGCTgg                                                                                                                                                      >  2:195977/3‑250 (MQ=255)                                                                                                cAAACGAAACGGAAGCATAAAAGCTCACCGGTATTCGTGGTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGTGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGaaaaa                                                                                                                                              <  1:517618/251‑1 (MQ=255)                                                                                                     gaaacgGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCAtt                                                                                                                                         <  1:219781/251‑1 (MQ=255)                                                                                                     gaaacgGAAGCAGAAAAGCCCCCCGGTATTCGTGTTGAAAAGGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACGTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAATAAAAACCAtt                                                                                                                                         <  2:321278/251‑1 (MQ=255)                                                                                                                aGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATtgctg                                                                                                                                                                                                >  1:237109/1‑185 (MQ=255)                                                                                                                aGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATtgctg                                                                                                                                                                                                <  2:237109/185‑1 (MQ=255)                                                                                                                   aaaGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCAGTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCAGTGCCAGAGGCGATTc                                                                                                                           <  2:197942/251‑1 (MQ=255)                                                                                                                         cACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGttt                                                                                                                       <  2:509420/249‑1 (MQ=255)                                                                                                                            cGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGGCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACAGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGccc                                                                                                                  <  2:518411/251‑1 (MQ=255)                                                                                                                                      gtTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGc                                                                                                        >  1:466266/1‑251 (MQ=255)                                                                                                                                            aaTGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGAt                                                                                                  >  1:141169/1‑251 (MQ=255)                                                                                                                                             atgatgAAGATGCAACGAAGGCGGGGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGACTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGAtt                                                                                                 <  1:18401/251‑1 (MQ=255)                                                                                                                                             atgatgAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGAtt                                                                                                 <  1:311350/251‑1 (MQ=255)                                                                                                                                                atgaAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACAATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATGCGTTTTGCCCATGCTGCCGCTGCGATTGcc                                                                                              <  1:175247/251‑1 (MQ=255)                                                                                                                                                  gaAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCg                                                                                             <  2:103225/250‑1 (MQ=255)                                                                                                                                                  gaAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAA‑GGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGt                                                                                            >  1:166958/1‑250 (MQ=255)                                                                                                                                                      aTGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTAATGTACTGATTACTTTAGGAAGACGTGGGGAATGGGATAGAGTGAATGATGCAGGTAAGCGCGTTCCTGGATTCCGGGCGCAGGCTGTCGATACCGTTGCTGCGGGCGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAGACAGTGCCACCGGGGATTCGTGTTGCCCATGTTGCCGCTGCGATTGCCGGAac                                                                                         >  2:53102/1‑250 (MQ=255)                                                                                                                                                          aacGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGACGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGt                                                                                     >  1:363530/3‑250 (MQ=255)                                                                                                                                                          aacGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGt                                                                                     >  1:437929/3‑250 (MQ=255)                                                                                                                                                           acGAAGGCGGGGCAGGTACTGCATGCAAAAGGTATCCGTACTGTACTGATTACTTAAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGAGACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTGTGCCCCTGCTGCCGCTGCGATTGCCGTAACACGTaa                                                                                   <  2:312383/250‑1 (MQ=255)                                                                                                                                                                  cggcgCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTc                                                                                                                                                                                                                            >  1:285777/1‑107 (MQ=255)                                                                                                                                                                  cggcgCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTc                                                                                                                                                                                                                            <  2:285777/107‑1 (MQ=255)                                                                                                                                                                        aGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGAGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGACGCTGCGATTGCCGTAACACGTAAAGGCCCAAAACCt                                                                      >  2:318781/1‑251 (MQ=255)                                                                                                                                                                            aCTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCg                                                                  >  1:243475/1‑251 (MQ=255)                                                                                                                                                                                                gtgctgtacttATTACCTTAGGAAGTCGTGGGGTATGGGCTAGCGGGAATGAGGAAGGTCAGCGCGTTCCTGGAGTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGTAAGAAAAACCATTGCCAGAGGCGATTCGGTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGGACCGTGGCGTGAAGAGATc                                              <  2:568144/248‑1 (MQ=255)                                                                                                                                                                                                     gtactgATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGc                                         >  1:162661/1‑251 (MQ=255)                                                                                                                                                                                                         tgATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCAttt                                     >  1:263447/1‑251 (MQ=255)                                                                                                                                                                                                          gATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCAttt                                     <  1:107528/250‑1 (MQ=255)                                                                                                                                                                                                            ttACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGCTTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCg                                                                                                                          >  1:398734/1‑163 (MQ=255)                                                                                                                                                                                                            ttACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATGCCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATGGCCTGAGGCGATTCg                                                                                                                          <  2:398734/163‑1 (MQ=255)                                                                                                                                                                                                             tACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCAttttt                                   >  1:16039/1‑249 (MQ=255)                                                                                                                                                                                                                 ttAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGAc                               <  2:437929/249‑1 (MQ=255)                                                                                                                                                                                                                    ggAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGCTACCATTGCTGCCGGAGCTACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCGTGCTGCCGCTGCGATTGCCGTAaca                                                                                        >  1:24208/1‑189 (MQ=255)                                                                                                                                                                                                                     gAAGTCGGTGTGTATGGGCTAGCGTGAATGATGAAGGGCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTTCCGCTGCGATTGCCGTAACACGTAAAGTCGCACAACCTTCCGTACCGGGGCGTGAAGAGATCGACGCATTTTTAGACAGGCag                         <  2:526368/251‑1 (MQ=255)                                                                                                                                                                                                                      aaGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGc                           >  1:45117/1‑248 (MQ=255)                                                                                                                                                                                                                          cGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGCCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTg                    >  1:413491/1‑251 (MQ=255)                                                                                                                                                                                                                          cGTGGTGTAGGGGCTAGCTTGAATGATTAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGGCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGTGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTg                    <  2:389135/251‑1 (MQ=255)                                                                                                                                                                                                                             ggtgGATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACg                 <  2:45117/251‑1 (MQ=255)                                                                                                                                                                                                                              gtgtATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGc                >  1:319532/1‑251 (MQ=255)                                                                                                                                                                                                                                gtATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCtt              >  1:461333/1‑251 (MQ=255)                                                                                                                                                                                                                                        tAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGGGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGCTTGCCGTAACAAGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTAcc       >  1:37090/1‑249 (MQ=255)                                                                                                                                                                                                                                               aaTGATGAAGGTCAGCGCGATCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGAGTGAAGAGATCGACGCCTTTTTAGACAGGCAGAGGTGACGCTTGGCTACCATGaa  >  2:566372/1‑248 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    AATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAGCGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGGTGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAA  >  NC_000913/3937755‑3938240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑