Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,937,909	G→A	A206T (GCG→ACG)	rbsK →	ribokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,937,909	0	G	A	100.0%	214.5 / NA	64	A206T (GCG→ACG)	rbsK	ribokinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/36);  total (28/36)

AACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAGCGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGGTGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAAC  >  NC_000913/3937676‑3938153                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                      aaCGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTa                                                                                                                                                                                                                                      <  2:558596/251‑1 (MQ=255)  aCGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTAc                                                                                                                                                                                                                                     <  1:75177/251‑1 (MQ=255)   ggggAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTACGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACt                                                                                                                                                                                                                                    <  2:176202/248‑1 (MQ=255)   cGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACt                                                                                                                                                                                                                                    <  1:571494/251‑1 (MQ=255)       aGCGTATTGCCAGCGTGTCAGCAGTAATAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTGGCGCTTAACCCGGCTCCGGCTCGCGAACTGCCTGACGAACTGCTGGCGCTGGTGGACATTATGACGCCAAACGAAACGTAAGCAGAAAAGCTCAC‑CGGTATTCGTTTTGAAAATGATGAAGATGCAACGAAGTCGGCGCAGGTACTGCAt                                                                                                                                                                                                                                <  2:32393/251‑1 (MQ=255)         cGTATTGACAACGCGTCAGCATTATTACTGCAGCTGGAATCACCACTCGCAAGTGTGATGGCAGCGGCGAAACTCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGAGGAACTTATTACGCCACACGAACCGGGAGCAGAAAAGCTCAC‑CGGTCTTCGTGTTGGAAATGATGAAGAATCAACGCAGGCGGCGCGGGTACTggctg                                                                                                                                                                                                                               >  2:219781/1‑246 (MQ=255)           tATTGCCAACGAGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGa                                                                                                                                                                                                                              <  1:327880/249‑1 (MQ=255)              tGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGCAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGaaaa                                                                                                                                                                                                                           >  1:441191/1‑249 (MQ=255)               gCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAgg                                                                                                                                                                                                                        <  1:486547/251‑1 (MQ=255)                         cAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTAAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCgtact                                                                                                                                                                                                             >  1:515756/1‑251 (MQ=255)                          aGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCgtactg                                                                                                                                                                                                            <  2:88940/251‑1 (MQ=255)                          aGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCgtactg                                                                                                                                                                                                            <  1:21982/251‑1 (MQ=255)                          aGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCgtactg                                                                                                                                                                                                            <  1:307985/251‑1 (MQ=255)                            cATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCgtactgta                                                                                                                                                                                                          <  1:28914/251‑1 (MQ=255)                                   aaggCAGCTGGAATCTCCGCTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAATACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTa                                                                                                                                                                                                   <  2:266071/248‑1 (MQ=255)                                   aaTGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGCCTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGAGCTGCTGGCCATTCTTACGCCAAACGAAACGGAAGCAGAAAAGATCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCCGGTAATGCATGAAACAGGTAT‑CCGTACTGTAATGAtt                                                                                                                                                                                                    >  2:107528/1‑250 (MQ=255)                                           tGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAgg                                                                                                                                                                                            >  1:321278/1‑250 (MQ=255)                                               aTCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGCACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTc                                                                                                                                                                                       >  1:526368/1‑251 (MQ=255)                                                 caccacTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGGGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTAATGATTACTTTAGGAGGTCgg                                                                                                                                                                                     >  1:19092/1‑250 (MQ=255)                                                         gAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCCCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGC‑CACACGGTATTCGTGGTGAAAATGATGAAGATTCAACGAAGGCGGCGCCGGGACTGCATGAAAAAGGTAT‑CCGTACTTGACTGATTACTTTTGGAATGCGTGGTGTATg                                                                                                                                                                             >  2:303335/1‑251 (MQ=255)                                                                   tGGCAACGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAAATTCCTGACGAACTGCTGGCCCTGGTGGACATTATTACGCCAAACGAAACGGAAGAAGAAAAGCTAAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGAGGGGACGGTACTGCATGAAAAAGG‑ATGCCGTACTGTACCAGTTAGTTTGGGAAGACGTGGTGGATGGGCTAGCGTg                                                                                                                                                                   >  2:564403/1‑251 (MQ=255)                                                                                aaTCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGCCGAAATGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAACAGAAAAGCTCAC‑CGGTAATCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGAGCAGGTACTGCATGGAAAAGGTAT‑CCGTACTGTAATTCTTACTTTAGGAAGTCGTGGTGGATGGGGTAGCGTGAATAATGAAGGtt                                                                                                                                                      >  2:224918/1‑250 (MQ=255)                                                                                aaTCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTc                                                                                                                                                      <  1:303335/251‑1 (MQ=255)                                                                                     cccATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAgcgc                                                                                                                                                 >  1:438117/1‑251 (MQ=255)                                                                                      ccATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACTAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGcgcg                                                                                                                                                <  1:139447/251‑1 (MQ=255)                                                                                      ccATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGcgcg                                                                                                                                                >  1:304973/1‑251 (MQ=255)                                                                                          cAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTcc                                                                                                                                            <  1:355521/251‑1 (MQ=255)                                                                                                   aCTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCg                                                                                                                                   <  1:60390/251‑1 (MQ=255)                                                                                                         ggtGCGCTTACCCCGGCTCCGGCTCGCGAAATTCCTTAAGACCTGCTGGCGCTGGTGGCCATTATTAAGCCCAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGGTGCAAAGAAGGCGGCGCAGGTACTGCATGAAAAAGGGAT‑CCGTAATGTACTGATTGTTCTAGGAGGTCGTGGGGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCAGGATTCCgggcga                                                                                                                              >  2:526301/3‑247 (MQ=255)                                                                                                         gTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATAATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGGCGTGGTGAATGGGATAGCGTGGATTATGAAGGTCAGCGCGTTCCTGGATTCCgggggcg                                                                                                                             >  2:311350/1‑247 (MQ=255)                                                                                                           tgctctgaaCCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAg                                                                                                                            <  2:521427/243‑1 (MQ=255)                                                                                                              gcTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTg                                                                                                                        >  1:160003/1‑251 (MQ=255)                                                                                                               gtttaCCCGGCGCCGGCTCGCGAACGTCCTGACGAACTGCTGGCGCTGGTGGACAATATTACGCCAAACGAAACGGAAGCAGAAAAGCGCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGGACTGATTACTTTAGGAAGTCGTGGTGTAGGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCt                                                                                                                         <  2:183251/245‑1 (MQ=255)                                                                                                                 tAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGTTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCAGGAAAAAGGAAT‑CCGTACTGTACTGGTTACTTTAGGAAGTCGTGGAGGATGGGCTAGAGTGAATGATGGAGGTCAGAGCGTTCCTGGATTCCGGGGGCAGGCt                                                                                                                         >  2:440601/1‑247 (MQ=255)                                                                                                                 tAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCt                                                                                                                         <  1:440601/247‑1 (MQ=255)                                                                                                                  aaCCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCg                                                                                                                     <  1:224918/250‑1 (MQ=255)                                                                                                                     ccggctccggctCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATAc                                                                                                                 <  1:458128/251‑1 (MQ=255)                                                                                                                           ccggctcGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATtgc                                                                                                           >  1:568144/1‑251 (MQ=255)                                                                                                                            cggagctcgaacatacTGACGAACTGCGTGCGTTGTTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGGGTTGAAAAGGATGAAGATTCAACGATGTCGTCGCAGGTACTGCATGAAAAAGGTAT‑CCGTTCAGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTTAAGGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTCTTGATACCATtgct                                                                                                          <  2:421289/236‑1 (MQ=255)                                                                                                                                 cgcgAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCg                                                                                                      <  2:134928/250‑1 (MQ=255)                                                                                                                                          ccTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCt                                                                                             <  1:456662/250‑1 (MQ=255)                                                                                                                                           cTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGACGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCttt                                                                                           >  1:452129/1‑251 (MQ=255)                                                                                                                                              gcGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAAc                                                                                        <  2:150827/250‑1 (MQ=255)                                                                                                                                                gAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACgg                                                                                      >  1:435863/1‑251 (MQ=255)                                                                                                                                                gAACTGCTGGCGCTGGTGAACATTATTACGCAAAACGCAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACgg                                                                                      >  1:159024/1‑251 (MQ=255)                                                                                                                                                               gtggACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGc                                                                       >  1:164596/1‑251 (MQ=255)                                                                                                                                                                   aCATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTg                                                                   >  1:197942/1‑251 (MQ=255)                                                                                                                                                                        aataCGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGCAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGGTTCCGGGTGCAGGCTGTCGATACCAGTGCTGCCGGAGTTGCCTTTACCGGTGCGATAATCACGGACTTGCTgg                                                               >  2:195977/3‑250 (MQ=255)                                                                                                                                                                               cAAACGAAACGGAAGCATAAAAGCTCAC‑CGGTATTCGTGGTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGTGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGaaaaa                                                       <  1:517618/251‑1 (MQ=255)                                                                                                                                                                                    gaaacgGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCAtt                                                  <  1:219781/251‑1 (MQ=255)                                                                                                                                                                                    gaaacgGAAGCAGAAAAGCCCCC‑CGGTATTCGTGTTGAAAAGGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACGTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAATAAAAACCAtt                                                  <  2:321278/251‑1 (MQ=255)                                                                                                                                                                                               aGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATtgctg                                                                                                         <  2:237109/185‑1 (MQ=255)                                                                                                                                                                                               aGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATtgctg                                                                                                         >  1:237109/1‑185 (MQ=255)                                                                                                                                                                                                  aaaGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCAGTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCAGTGCCAGAGGCGATTc                                    <  2:197942/251‑1 (MQ=255)                                                                                                                                                                                                        cAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGttt                                <  2:509420/249‑1 (MQ=255)                                                                                                                                                                                                            cGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGGCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACAGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGccc                           <  2:518411/251‑1 (MQ=255)                                                                                                                                                                                                                      gtTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGc                 >  1:466266/1‑251 (MQ=255)                                                                                                                                                                                                                            aaTGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGAt           >  1:141169/1‑251 (MQ=255)                                                                                                                                                                                                                             atgatgAAGATGCAACGAAGGCGGGGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGACTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGAtt          <  1:18401/251‑1 (MQ=255)                                                                                                                                                                                                                             atgatgAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGAtt          <  1:311350/251‑1 (MQ=255)                                                                                                                                                                                                                                atgaAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACAATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATGCGTTTTGCCCATGCTGCCGCTGCGATTGcc       <  1:175247/251‑1 (MQ=255)                                                                                                                                                                                                                                  gaAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCg      <  2:103225/250‑1 (MQ=255)                                                                                                                                                                                                                                  gaAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAA‑GGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGt     >  1:166958/1‑250 (MQ=255)                                                                                                                                                                                                                                      aTGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTAATGTACTGATTACTTTAGGAAGACGTGGGGAATGGGATAGAGTGAATGATGCAGGTAAGCGCGTTCCTGGATTCCGGGCGCAGGCTGTCGATACCGTTGCTGCGGGCGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAGACAGTGCCACCGGGGATTCGTGTTGCCCATGTTGCCGCTGCGATTGCCGGAac  >  2:53102/1‑250 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                      AACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCAC‑CGGTATTCGTGTTGAAAATGATGAAGATGCAGCGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAT‑CCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGGTGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAAC  >  NC_000913/3937676‑3938153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑