Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,927,292	T→C	D46D (GAT→GAC)	asnA →	asparagine synthetase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,927,292	0	T	C	100.0%	183.7 / NA	52	D46D (GAT→GAC)	asnA	asparagine synthetase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/19);  total (33/19)

TTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGT  >  NC_000913/3927052‑3927532                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 tttAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTAGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGACGATTCTTAGCCGTGTGGGGGATGGCCCGCAGGACAACTTGTCgg                                                                                                                                                                                                                                        >  1:272313/1‑251 (MQ=255) tttAATGAATCAAAAGTGAGTTAGGCTGTTTATTGAATTATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCg                                                                                                                                                                                                                                         <  1:551584/250‑1 (MQ=255)   tAATGAATCATAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCTTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCggg                                                                                                                                                                                                                                       <  1:345700/250‑1 (MQ=255)    aaTGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCt                                                                                                                                                                                                                                     >  1:201388/1‑251 (MQ=255)     aTGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTATTCATAAGCAACAGGTCGCGGGAGTATAAAAACTGACAACCGCTTACATTGACAAAAAACGTAAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCCACTGGAAGAACGTCTGGGGCTGATGGCAGACAAGGCGCCGATTCTTATCCGGGTGGGGGAGGGCACGCCGGACCACTTTTCGGGCtt                                                                                                                                                                                                                                    >  2:487962/1‑250 (MQ=255)         aTCAAAAGTGAGTTAGGCTATTTATTGAATGATTCTTGCATGTGTGTCAGTTTTTGTTGCTTAATCATAAGCAACCGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAAAAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGa                                                                                                                                                                                                                                 >  1:427820/1‑250 (MQ=255)           cAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGaaa                                                                                                                                                                                                                               >  1:208933/1‑250 (MQ=255)            aaaaGGGAGTTAGGCTTTTTATTGGATGATTATTGCATGTGTGTAGGTTTTTGTTGCTTAATCATAAGCAACAGGACGAAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTAACTTTTCTCGTCAACTGGAAGTACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGGGTGGGGGATGGCAAGCAGGACAACTTGTCGGGCTGTGaaaa                                                                                                                                                                                                                              >  1:475352/1‑250 (MQ=255)                  gAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCCGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGt                                                                                                                                                                                                                        <  1:565050/250‑1 (MQ=255)                         gCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTa                                                                                                                                                                                                                 >  1:50799/1‑250 (MQ=255)                                aTTGCATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACCGGACGCAGGAGTATAAAAAATTAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACAGGCAGAACGTCAGGGTCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGTGGTTGGCACGCAGGCCAACTGGTCGGGCTGTGAAAAATCGGTGAAGGAAAAAGTgg                                                                                                                                                                                                          >  2:109967/1‑249 (MQ=255)                                       gATTATTGGATGGGTGGCGGTTTTTGTTGCTTAATCATAATCAACAAGACGCAGGAGTAAAAAAAATGAAAACCGCTTACATTGCCAAACAACTACAAATTTGCTGCGTGAAATCTCACTTTTCGCGGCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGAAAGCAGGACAACTTGGCGGTCTGTTAAAAAGCGGTGCAGGTAAAAGGGAAAGctct                                                                                                                                                                                                   <  2:562557/250‑1 (MQ=255)                                       gATTATTGCATGTGTGACGGTTTTTGTTGCTTAAGCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTGCGTGAAATCTCACGTTTCTCGTCAACTGGAAGAACGGCTGGGGCTGATCGAAGGCCAGGCGCCGATTCTCAGACGTGTGGGGGATGTCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGTTGCAGGTATAAGCGAAATctct                                                                                                                                                                                                   <  2:570791/250‑1 (MQ=255)                                        attattGCATGTGTGTCGGTTTGTGGTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGc                                                                                                                                                                                                 <  2:135396/251‑1 (MQ=255)                                               cATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGc                                                                                                                                                                                          >  1:394830/1‑251 (MQ=255)                                                    gtgtCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGATGTGAAAAAGCGGTGCAGGTAAAGGTGAAAGCTATGCCTGATGCCCAGt                                                                                                                                                                                     >  2:31596/1‑251 (MQ=255)                                                     tgtCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGtt                                                                                                                                                                                    >  1:225132/1‑251 (MQ=255)                                                               tgttgCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGCTTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGGTGCCCAGTTCGGAGTGGtt                                                                                                                                                                          >  2:80338/1‑251 (MQ=255)                                                                       aaTCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTACCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACt                                                                                                                                                                  >  1:247097/1‑251 (MQ=255)                                                                           aTAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCg                                                                                                                                                              <  1:539508/251‑1 (MQ=255)                                                                             aaGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGa                                                                                                                                                             >  2:463110/1‑250 (MQ=255)                                                                                cAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTACTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGGGGGGGATGGCACGCAGGACCACTTGTCGGGCTGTGAAAAAGCGGGGCAGGAAAAAGTGAAAGCTCTGCCTGATACCACGTACGAAGGGGATCATTCAcgggagacgtg                                                                                                                                                         >  2:510324/1‑241 (MQ=255)                                                                                          cAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTc                                                                                                                                                 >  1:407775/1‑249 (MQ=255)                                                                                                   aaaaaaTGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCTCAGTTCGAAGTGGGTCAGTCACTGGCGAAGTGGAAACGTCAGACCTTAggg                                                                                                                                      <  1:487962/251‑1 (MQ=255)                                                                                                             aaCCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACt                                                                                                                            >  1:21608/1‑251 (MQ=255)                                                                                                                cGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTcc                                                                                                                         >  1:281527/1‑250 (MQ=255)                                                                                                                           ccaaaaagcGTCATATTAGCTTCGTGAAATCTCACTTTTCTCGTCCACTAGAAAAACGTCTGGGGCTGATCTAAGTCCAGGCGCCGCTTCTGAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTATGAACCGGCGGGGCAGGGCAAAGTGAAAGCTCTGCCTGCTGACAAGTTCGCAGTGGATCATTACCGGGCGACGAGGAAACATTAGACCTTAGGGATACACAACTTcggctaggccta                                                                                                               >  2:471801/9‑239 (MQ=255)                                                                                                                                 aacGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTg                                                                                                        >  1:504148/1‑251 (MQ=255)                                                                                                                                     tCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTATGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGAGAAGGGCTGtcc                                                                                                     >  2:559716/1‑248 (MQ=255)                                                                                                                                      cAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTacac                                                                                                   >  1:518616/1‑251 (MQ=255)                                                                                                                                         aTTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGc                                                                                                 >  1:291331/1‑250 (MQ=255)                                                                                                                                             gCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGCCCTTAGGGCa                                                                                                                                    >  2:132210/1‑211 (MQ=255)                                                                                                                                             gCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCa                                                                                                                                    <  1:132210/211‑1 (MQ=255)                                                                                                                                                    gAAATCTCCCTTTTCTCGTCCACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGAAGGTAAAAGTGAAAGCTCTGCCTGATGCCCCGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAGACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACGTGAAAcccc                                                                                     >  2:205455/1‑251 (MQ=255)                                                                                                                                                            ccTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGcccc                                                                             <  1:463110/250‑1 (MQ=255)                                                                                                                                                                ttCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGAt                                                                          <  1:311424/250‑1 (MQ=255)                                                                                                                                                                 tcccGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATg                                                                         <  1:287382/247‑1 (MQ=255)                                                                                                                                                                                gaaCGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGc                                                                                                 <  2:49042/211‑1 (MQ=255)                                                                                                                                                                                gaaCGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGc                                                                                                 >  1:49042/1‑211 (MQ=255)                                                                                                                                                                                   cGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGtt                                                      >  1:545901/1‑251 (MQ=255)                                                                                                                                                                                          ggCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGCCAACTTGTCGGGCTGTGAACAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGAGCGCAGGGCTGTACACGCACATGCAAGCCCTTCGCCCCGCTGAAGACCGTCTTTCTCCGTTGccct                                                 >  1:531379/1‑246 (MQ=255)                                                                                                                                                                                             tGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGCTGAAGACCGTCTTTCTCCGTTGCACTCGGTc                                            >  2:288151/1‑251 (MQ=255)                                                                                                                                                                                                    aGTCCAGGTGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGGCGGGTTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCACTCACTGGCGAAGTGGAATCGTCATACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGTCCTGCGCTCCGATGAAGTCCGTCTTTCCCCGTTGCACTCGGTCTAGGTTg                                     <  2:208933/251‑1 (MQ=255)                                                                                                                                                                                                    aGTCCAGGCGCCGATTCTTCGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGACAGCTCTGCCTGATGCCCAGTTCGACGTGGTTCATTCACTGGCGAAGTGGAAACGTAAGACCTTAAGGCAACACGACTTCCTCGCGACGGGAGGGCTGTACACGCACATGAAAGACCTTCGCCCCGCAGAAAACCGTCTTTCGCCGTTGACCTCGGTCTATGttt                                     >  2:531101/1‑250 (MQ=255)                                                                                                                                                                                                      tCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGt                                                       <  1:570169/231‑1 (MQ=255)                                                                                                                                                                                                      tCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGt                                                       >  2:570169/1‑231 (MQ=255)                                                                                                                                                                                                      cccAGGCGCCGTTTCGTAGCCGTTTGGGGGATGCCACGCATGACATCTTGTCGGGCTGTGAAAAAGCTGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTGCATTCACTGGCGAAGGGGAAACGGCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCACCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGAc                                   <  2:495772/250‑1 (MQ=255)                                                                                                                                                                                                                                 gggATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGAAATGGGc        >  1:316702/1‑251 (MQ=255)                                                                                                                                                                                                                                   gATGGCACGAAGTACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCGCGGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGAGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCAGTTGCACTCGGTCGATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGa      <  2:281527/251‑1 (MQ=255)                                                                                                                                                                                                                                    aTGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGCCTGGGAACGCGTAATGGGCGAc     >  1:275679/1‑251 (MQ=255)                                                                                                                                                                                                                                    aTGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGAc     <  1:288151/251‑1 (MQ=255)                                                                                                                                                                                                                                       gCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCATGGAAAAGTGAAAGTTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGTCTTCAGCGCGGGCGAAGGGCTGTACCCGCACATGAAAGCCCTTCGCCACGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGt  <  2:8785/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 TTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGGAACGCGTAATGGGCGACGGT  >  NC_000913/3927052‑3927532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑