Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,725,176	T→G	E48A (GAG→GCG)	glyQ ←	glycine‑‑tRNA ligase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,725,176	0	T	G	100.0%	189.9 / NA	61	E48A (GAG→GCG)	glyQ	glycine‑‑tRNA ligase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (36/25);  total (36/25)

AGCGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCAC‑CTGGAA‑CTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAG‑GATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCAT  >  NC_000913/3724942‑3725421                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                       aGCGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCAC‑CTGGAA‑CTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCAt                                                                                                                                                                                                                                        >  1:409048/1‑251 (MQ=255)         gTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGCATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCAC‑CTGGAA‑CTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGTTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCAGGCCCCAGCGCGCGCAGACAGGGCAGTGGGTg                                                                                                                                                                                                                                  >  2:543146/1‑249 (MQ=255)           tttCCCAGTTATCTTCCACGAAACGGATGTCGGGAATAGTGGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCGGAATATTGTCCGGCGATGGCTTAATGACCAC‑CTGGAA‑CTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCACCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTGCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTgaga                                                                                                                                                                                                                               <  2:476813/250‑1 (MQ=255)                cAGTTATCTTCCACGAAACGGATGCCGTGAATAGTCGGGTACATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCCC‑CTGGAA‑CTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCCTCGGTCGGGCGACGAGAAGGCTGACCATAAGCAGCCGCCCTCGGTTCTGGCCCCCGCGCGCGCCGCCAGGTCCTTgggggcgagggtcc                                                                                                                                                                                                                         >  2:261746/1‑240 (MQ=255)                     aTCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCAC‑CTGGAC‑CTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCgcgc                                                                                                                                                                                                                    >  1:271409/1‑251 (MQ=255)                        ttCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCAC‑CTGGAA‑CTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGCGGTTCCCGCGCCg                                                                                                                                                                                                                  >  1:502627/1‑250 (MQ=255)                              gAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCAC‑CTGGAA‑CTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGGTCCCGAGCAGACTTcc                                                                                                                                                                                                            >  1:542877/1‑250 (MQ=255)                                accGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCAC‑CTGGAA‑CTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATg                                                                                                                                                                                                         <  1:543146/249‑1 (MQ=255)                                   tgATGTCGTGAATAGTCGGGTCCATGGCCGGCTCTTTCGGAGAACCGAGGTACAGATCCTGAATATTGTCCGGCGATGGCTTAATGCCCAA‑CTGGCA‑CTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGTGCGACGAGAAGGCTGAACATAAGCAGCCGCCGTCGGTTCTGGCCCCAGCGCTCGCAGCCAGGTCATTGGGAGAGAGGTTCCCGCGCCGCCTTCCgtgtac                                                                                                                                                                                                      >  2:76396/2‑249 (MQ=255)                                           tgaatatgagggTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGATACTGAATATTGTCCGGCGATGGCTTAATGACCAC‑CTGGAA‑CTGATAGTCGTGCTGTAAACGGTTGGGGTTTTCGACATAGCGACACTCGGTCGGGCGAAGACAAGACTGAACATAAACAGCCGCCCTCAGTTCTGGCCCCAGCGAGCGAAGACAGTTCATTGGGTGCGTGGTTCCCGCGACGACTTTCATGCACAATGGttt                                                                                                                                                                                              >  2:196393/10‑250 (MQ=255)                                                       tCCATGCCCAGATCTTTCAGAGAACCGAGGTACAGCTCCTGAATCTTGTCCGGCGACCGCTTAATGACCAC‑CTGGAC‑GTGATAGTCGTGCTGTAAACGGTTGGGGTTTTCGCCATAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATCAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGCCAGGACATTGGGGGCGAGGATCCAGAGCCGACTTCCATGCCCAATGGTTGAACAATggggcg                                                                                                                                                                                  >  2:331412/1‑247 (MQ=255)                                                         cATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCAC‑CTGGAA‑CTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGc                                                                                                                                                                                >  1:211728/1‑251 (MQ=255)                                                              ccAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCAC‑CTGGAA‑CTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTgg                                                                                                                                                                           >  1:410087/1‑251 (MQ=255)                                                                        cagatacccggggtacagcctatGAATATTGTCCGGTGATGGCCTAATGACCTC‑CTGGAA‑CTGAGAGTAGTGCTGTAAACGGTTGGGTGTTCCGACGTAGCGACCATCGGTCGGGTGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCGGCGCGCGCAGACAGGTCATTGGGTGAGATGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCACTGGCGAGCCCAGt                                                                                                                                                                 <  2:433429/229‑1 (MQ=255)                                                                             aaCCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCAC‑CTGGAA‑CTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATcc                                                                                                                                                            >  1:60257/1‑251 (MQ=255)                                                                                 gAGGTACCGCTACTGAATATTGTCCGGCGTTGGCTTAATGACCAC‑CTGGAA‑CTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATAGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCACAGCGCGCGCAGACAGGTCATTGGGCGAGAGGATCCCGAGCCGACTTCCATGTCCAATGGTTGAACACTGGGGCAGCCCTGGCGAGCCCAGTACTCCTGt                                                                                                                                                         >  2:516843/1‑250 (MQ=255)                                                                                     tACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCAC‑CTGGAA‑CTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGt                                                                                                                                                    >  1:420796/1‑251 (MQ=255)                                                                                         gCTCCTGAATATTGTCCGGCGATGGCTTAATGACCAC‑CTGGAA‑CTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCCGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAAAAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTagg                                                                                                                                                      >  2:361403/1‑243 (MQ=255)                                                                                         gCTCCTGAATATTGTCCGGCGATGGCTTAATGACCAC‑CTGGAA‑CTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAg                                                                                                                                                      <  1:361403/245‑1 (MQ=255)                                                                                              tGAATATTGTCCGGCGATGGCTTAATGACCAC‑CTGGAA‑CTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGCGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAG‑GATCa                                                                                                                                           >  2:171526/1‑250 (MQ=255)                                                                                               gAATTTTGTCCGGCGTTGGCTTAATGAC‑ACTCTGGAA‑CTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCTGTAGCGACCATCGGTCCGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCTTGTCCAATGGTTGAACAATTGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGGCAT‑GATCAAg                                                                                                                                         <  1:421033/251‑1 (MQ=255)                                                                                               gAATATTGTTCGGCGGCGGCTTAATGACCAC‑CGGGAAACT‑ATAGTAGTGCGGTAATAGCGTGGTGTTGTCGCCGTTGCGACAATCGGTCGGTCGACGAGGAGGCTGAACAGAAGCCGCCGCCATGGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCATCCCTTGAGGGCCCAGTAATCCTGTAAGGCCAG‑GATCAAg                                                                                                                                         <  2:63851/251‑1 (MQ=255)                                                                                                 atatgggccGGCGGTGGCTTAATGACCCC‑CAGGAA‑CTGATAGTAGTGCTGGAAACGGTTGGGGTGTTCGCCGTAGCGACCATCGGTCGGGTGACGAGAAGGCTGAACATAAGCAGCCGCCAGCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACATCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCGGTAATCCTGTAAGGTCAG‑GATCAAGcc                                                                                                                                       <  2:184560/244‑1 (MQ=255)                                                                                                   atTGTCCGGCGATGGCTTAATGACCAC‑CTGGAA‑CTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCAGTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAG‑GATCAAGccc                                                                                                                                      <  2:240002/250‑1 (MQ=255)                                                                                                      gTCCGGCGATGGCTTAATGACCAC‑CTGGAA‑CTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAG‑GATCAAGCCCTgg                                                                                                                                   >  1:91081/1‑250 (MQ=255)                                                                                                        ccGGCGATGGCTTAATGACCAC‑CTGGAA‑CTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAGGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAG‑GATCAAGCCCTGGa                                                                                                                                  >  1:555584/1‑249 (MQ=255)                                                                                                          ggCGATGGCTTAATGACCAC‑CTGGAA‑CTGGTAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGAAGCCGCCATCGGTTCTGGCCGCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCCCCAGCTTCCATGTCAAAAGATTGAACAATGGTGCAGCCCTGTCGAGCCCAGTAATCTGGTAAGGTCAG‑GATAAAGCCCTGGAAggg                                                                                                                              >  2:198557/1‑250 (MQ=255)                                                                                                                    tAATGACCAC‑CTGGAT‑CTGATAGTAGTGCTGTAAACGGTTGGGGTGTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCAATCGGGTCTGGCCCCAGCGCGCGCAGACATGTCATTGGGTGAGAGGTTCCCGCGCCGACTGCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAG‑GATCAAGCCCTGGAAGGTCCTGGTAt                                                                                                                      <  2:433328/249‑1 (MQ=255)                                                                                                                    tAATGACCAC‑CTGGAA‑CTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGACGAAGCGACCATCGTACGGGCGACGAGAAGGCTGAACAAAAGCAGCCGCCATCGGTTCTGGCCCCAGCGAGCGCAGACAGGCCATTGGGTGAGAGGTTCCCGCGACGAATTACATGTCCATAGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGAAATCCTGTAAGGTAAG‑AGTCAAGCCCTGGCAGGTCCGGGTaac                                                                                                                     >  2:439246/1‑248 (MQ=255)                                                                                                                     aaTGACCAC‑CTGGAA‑CTGATAGTAGTGCTGGAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAG‑GATCAAGCCCTGGAAGGTCCTGGTATCa                                                                                                                    <  2:409048/250‑1 (MQ=255)                                                                                                                       tGACCAC‑CTGGAA‑CTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGCCTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAG‑GATCAAGCCCTGGAAGGTCCTGGATTCAAAc                                                                                                                 >  1:274961/1‑251 (MQ=255)                                                                                                                           cac‑cTGGAA‑CTGATAGTAGTGCTGTAAACGGTTGGGGGTTTCGCCGAAGAGACCATCGGTCGGGCGACGAGTAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCACGCGCCGACTTCCCTGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGACCAGTAATCCTGTAAGGTCAG‑GATAAAGCCCTGGACGGTCCTGGTATCAAACtttt                                                                                                             >  2:82028/1‑251 (MQ=255)                                                                                                                             c‑cTGGAA‑CTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAG‑GATCAAGCCCTGGAAGGTCCTGGAATCAAACTTTTGc                                                                                                           >  1:493011/1‑251 (MQ=255)                                                                                                                                 ggAA‑CTGATAGTAGTGCTGTAAACGGTTGGTGTTTCCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTTAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGTCAGGTCATTGGGTGAGATGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAG‑GATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCata                                                                                                        <  1:334463/251‑1 (MQ=255)                                                                                                                                   aa‑CTGATAGTAGTGCTGTAAACGGTTGGGGTGTTCGCCGTGGCGACCTTCGGGCGGGAGACGAGAAGGATGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCTCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAG‑GATCAAGCCCTGGAAGGTCCTGGTATCAATCTTTTGCAtatt                                                                                                      <  2:501362/251‑1 (MQ=255)                                                                                                                                         aTAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAG‑GATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTc                                                                                                 <  1:402014/251‑1 (MQ=255)                                                                                                                                            gtagtGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGCGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGAAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAG‑GTTCAAGCCCTGGACGGTCCGGGTATCACACTTTTGCATATTGTTTCGTg                                                                                              >  2:297676/1‑251 (MQ=255)                                                                                                                                              atggCTGTAAACGGGTGGGGTTTTCGACGTCTAGACCATCGGCCGGGCGTCGAGAAGGCTGAACATGAGCAGCCGCCATCTTTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTCCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGTCGAGCCCAGTAATCCTGTAATGTCAG‑GTTCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATGTCGttc                                                                                             <  2:380752/247‑3 (MQ=255)                                                                                                                                              agtGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAG‑GATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCt                                                                                            <  1:276567/251‑1 (MQ=255)                                                                                                                                                      aacaggTTGGGGTTTTCGCCGTAGCGACCATCGGTCAGGCGACAACACAGCTGAACATACGCGGCCGCCATCGTTTCTGGCCACAGCGCGCGCAGCCAGGAAATTGGGTGAGAGGATACCGCGACCACTTCAATAACCAATGGCTGAACAATGGGACAGCCCTGGAGCACCAAGTAATCCTGTAATCTCAGAGA‑CAAGCCCTGGACGATCCTAGAAACAACCTTTTACATCATATTTCTTGCGGGATACgg                                                                                    >  2:356651/5‑250 (MQ=255)                                                                                                                                                              ggggTTTTCGCCGTAGCGACCATCGGTCGGGCGCCGAGAAGGCTGAACATAAGCAGCCGACATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGGGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAG‑GATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATAAGGGTGGAttt                                                                            >  2:407992/1‑251 (MQ=255)                                                                                                                                                              ggggTTGTCGCCGTAGCGACCAGCGGGCGTGCGACGAGAAGGTTTAACATAAGCAGCCGCCATCGGTTCCGGCCCCAGCGCGCGCAGACAGGTCATTGGTTTAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCATTAATCCTGTAAGGTCAG‑GATCAAGCCCTGGAAGGTCATGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGTAttt                                                                            <  2:454924/251‑1 (MQ=255)                                                                                                                                                                ggTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAG‑GATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTa                                                                           >  1:183585/1‑250 (MQ=255)                                                                                                                                                                          gTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAG‑GATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGAt                                                                >  1:330859/1‑251 (MQ=255)                                                                                                                                                                           tAGCGACCATCGGTCGGGCGCCGAGAAGGCTGAACATAAGCAGCCGACATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCACAGTAATCCTGTAAGGTCAG‑GATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGTTTTAAAGAAAGGGATc                                                               >  2:92234/1‑251 (MQ=255)                                                                                                                                                                                    ccggtcggGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAG‑GATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATAccc                                                      <  1:297676/250‑1 (MQ=255)                                                                                                                                                                                    acggtcggGGGACGAGAAGGCTGAACATAAGCTGCCGCCATCGGTTCTTGCCCCAGCGCGCGAATACAGGTCATTGGGTGAGAGTTTCCCGCGCCGCCTTCCATGTCCAATGGTTTAACAATGGTGCAGCCCTGGCGAGCCCATTAATCCTGTAAGGTCAG‑GATCAATCCCTGGCAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATTAGTGTGGATTTAAAGTACTGGATCAGTATcccc                                                      <  1:203992/250‑1 (MQ=255)                                                                                                                                                                                     cggtcggGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAG‑GATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATAccc                                                      <  1:204013/250‑1 (MQ=255)                                                                                                                                                                                      ggtcggGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAG‑GATCAAGCCCTGGaa                                                                                                                                 >  1:216914/1‑174 (MQ=255)                                                                                                                                                                                      ggtcggGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTGCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAG‑GATCAAGCCCTGGaa                                                                                                                                 <  2:216914/174‑1 (MQ=255)                                                                                                                                                                                          gggCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAG‑GATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCtgga                                                <  1:366982/251‑1 (MQ=255)                                                                                                                                                                                           ggCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAG‑GATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCtggat                                               >  1:523795/1‑251 (MQ=255)                                                                                                                                                                                                cgaGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAG‑GATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAg                                          <  1:439246/251‑1 (MQ=255)                                                                                                                                                                                                    aaGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAG‑GATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGatat                                      >  1:161243/1‑251 (MQ=255)                                                                                                                                                                                                              aTAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAG‑GATCAAGCCCTGGAAGGTCCTGGTATCAAACGTTTGCATATTATTTCGTGCTGGAGACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGaa                            <  1:261746/251‑1 (MQ=255)                                                                                                                                                                                                                aaggaggcggcATCGGTTCTGGCCCCTGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGGCCTATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAG‑GATCAAGGCCTGGAAGGTCGTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTATGTATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATAGACAGTattaaac                          <  2:410525/241‑7 (MQ=255)                                                                                                                                                                                                                   cagcCGCCATCTGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAG‑GATCAAGCCCTTGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACggg                       <  2:460288/251‑1 (MQ=255)                                                                                                                                                                                                                       cgccATCGGGTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAG‑GATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAGGATATACAGTACGAAACGGGaaa                    >  2:109781/1‑250 (MQ=255)                                                                                                                                                                                                                        gccATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAG‑GATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGaaaa                   >  1:443878/1‑250 (MQ=255)                                                                                                                                                                                                                                  cTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAG‑GATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCtt         >  1:242096/1‑250 (MQ=255)                                                                                                                                                                                                                                        ccAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAG‑GATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCAt  >  1:418684/1‑251 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                       AGCGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCAC‑CTGGAA‑CTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAG‑GATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCAT  >  NC_000913/3724942‑3725421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑