Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,713,979 C→T P110S (CCG→TCG)  yiaC → peptidyl‑lysine N‑acetyltransferase YiaC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,713,9790CT96.7% 80.3 / NA 30P110S (CCG→TCG) yiaCpeptidyl‑lysine N‑acetyltransferase YiaC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (12/17);  minor base G (0/1);  total (12/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAGCGAATTACTGGCGTGACTGCATTCCGCTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAACCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTG‑TCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTT  >  NC_000913/3713736‑3714209
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                       
aaaGCGAATTACTGGCGTGACTGCATTCCGCTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGAt                                                                                                                                                                                                                                  <  1:125741/251‑1 (MQ=255)
    cGAATTACTGGCGTGACTGCATTCCGCTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAAt                                                                                                                                                                                                                              <  1:493754/251‑1 (MQ=255)
    cGAATTACTGGCGTGACTGCATTCCGCTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAAt                                                                                                                                                                                                                              >  1:406279/1‑251 (MQ=255)
                     tGCATTCCGCTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAggg                                                                                                                                                                                                             >  1:497288/1‑251 (MQ=255)
                         ttCCGGTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTTTCAGCATTATGGAAGGCCGATTTCTGGCAGAGATGTTTGTCGCATCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGttt                                                                                                                                                                                                          <  2:178971/250‑1 (MQ=255)
                                            cTATCTTGCCAACGCGCAACACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTG‑TCGATTGCGCAt                                                                                                                                                                                     >  1:210426/1‑251 (MQ=255)
                                                                            aagaagACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTG‑TCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGAtt                                                                                                                                                     <  1:43582/251‑1 (MQ=255)
                                                                                        aGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTG‑TCGATTGCGCATGGCAGGATGAAAccc                                                                                                                                                                      <  1:283551/222‑1 (MQ=255)
                                                                                           ttCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTG‑TCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCggtg                                                                                                                                      >  1:466197/1‑251 (MQ=255)
                                                                                                   gttGTCAGCATTATGGAAGGCCGATTTTTGGTAGCGATGTTTGTCTCACCGAAGGCCGTCAGGCGGTGTAATGGTAAGGCGCTGAGGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGTCGATAAATTTTTACCAGGCACAGGGTTTTCACATTG‑TCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGTTTCaaa                                                                                                                               <  2:501202/249‑1 (MQ=255)
                                                                                                    ttGTAAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCTCTGATGAAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTG‑TCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAAc                                                                                                                              <  2:97591/250‑1 (MQ=255)
                                                                                                      gTCAGCATGATGGAAGGCCGATTTCTCGCAGCGATGTTTGTCGCCCCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTG‑TCGATTGCGCATGGCAGGATGAAACCCAACTACCCACAGGGATTATGAGCTGGCCGGTGGTTCAAACga                                                                                                                            <  2:389118/250‑2 (MQ=255)
                                                                                                               aTGGAATGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAATGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCATCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTG‑TCGATTGCGCATGTCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAgtgt                                                                                                                   <  1:376825/250‑1 (MQ=255)
                                                                                                                   aaGGCCGATGTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTGTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTG‑TCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGtt                                                                                                              <  2:261755/251‑1 (MQ=255)
                                                                                                                     gtccGATTTCTGGCCGCGATTTTTGTCTCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGAGGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTG‑TCGATTTCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGGGTCAGTTcc                                                                                                            <  2:237884/249‑1 (MQ=255)
                                                                                                                                                   ccGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGACATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATAAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTG‑TCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAgc                                                                              >  1:362428/1‑251 (MQ=255)
                                                                                                                                                     gAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAACAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACTTTG‑TCGATTGCGCATGGCAGGATGAAACCCAACTACACACATGGATTATGAGCTGACCGGTGGTTCAAACGCTGTAAGTGTCCGTTCCGAACCGTTGTATTTTTCCAGCCATGCCAgcgc                                                                            >  1:510791/1‑251 (MQ=255)
                                                                                                                                                      aaGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTG‑TCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGcc                                                                           <  1:246734/251‑1 (MQ=255)
                                                                                                                                                      aaGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTG‑TCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGGTCCGGACCGTTGTATTTTTCCAGCCATGACAgcccc                                                                           >  2:36900/1‑248 (MQ=255)
                                                                                                                                                                ggCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGAGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATGTTTACCAGGCACAGGGTTTTCACATTG‑GCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCg                                                                 <  1:291954/251‑1 (MQ=255)
                                                                                                                                                                     ggTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAACAAAATCAATCGGCGATACATTTTTACCAGGCACAGGGTTTTCACATTG‑TCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGTTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCCGCCATGCCATCGCCTTATTACCCGcaccg                                                            >  2:83799/1‑249 (MQ=255)
                                                                                                                                                                        aTTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTG‑TCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCa                                                         <  1:79494/251‑1 (MQ=255)
                                                                                                                                                                             tAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCACAAAAATCAATCGGCGAtcaa                                                                                                                                                                                                                               >  2:341598/1‑78 (MQ=255)
                                                                                                                                                                             tAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATaaa                                                                                                                                                                                                                               <  1:341598/81‑1 (MQ=255)
                                                                                                                                                                                ggCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTG‑TCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGt                                                                                                               >  1:260005/1‑189 (MQ=255)
                                                                                                                                                                                ggCGCTGATGCAATATGAGCAGAAGCGCCATCCCCACCTGATACTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTAACCAGGCACAGGGTTTTCACATTG‑TCGATTGCGCATGGCAGGCTGAAACCCAACTACCAACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTACGTTTCCGTTCAGGACAGTTGTATTTTTCCCGCCATGCCAGCTCCGTATTACCCGCACAGCCATTcccccg                                                 >  2:545519/1‑249 (MQ=255)
                                                                                                                                                                                                gtgCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTG‑TCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCATCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGgaga                                 <  1:428421/251‑1 (MQ=255)
                                                                                                                                                                                                                 ccACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTG‑TCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGtt                >  1:516108/1‑251 (MQ=255)
                                                                                                                                                                                                                        aTGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTG‑TCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTgcg         <  1:451649/251‑1 (MQ=255)
                                                                                                                                                                                                                               aGGTTTATCAAAAAAATAAAGCGTCTGTAAATATTTGCCCGGATCAGGGTTTT‑TCATTGGTCACTTGCGCGCGGCAGGATGAAACCCACTTACCCACATGGATTATGAGCTGGCCCGTGGTCCAAACGCTGGAGGTGTCAGTTCCGGTCCGTTGTATTTTTCCCGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGtttt  <  2:224455/251‑1 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                       
AAAGCGAATTACTGGCGTGACTGCATTCCGCTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAACCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTG‑TCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTT  >  NC_000913/3713736‑3714209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑