Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,633,749	C→T	intergenic (‑1159/‑1092)	yhiJ ← / → yhiM	DUF4049 domain‑containing protein YhiJ/inner membrane protein YhiM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,633,749	0	C	T	100.0%	74.9 / NA	24	intergenic (‑1159/‑1092)	yhiJ/yhiM	DUF4049 domain‑containing protein YhiJ/inner membrane protein YhiM
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/12);  total (12/12)

AAAGTAAGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTATTTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGAAAACACAGATTTAACATAGCTATCAAACTTTTCCTTAGTAAACGTCTCTTTAGAGACATCCCAACGCCAGTCAGGTTTGAAATTACCACTAAGAAGAGTAAAGAAATTTCTCGCT  >  NC_000913/3633503‑3633991                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   aaaGTAAGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTATTTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGTCtat                                                                                                                                                                                                                                                <  1:430422/251‑1 (MQ=255)        ggATATATTTAACACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGAGCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTATTTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGTCTATATGcccg                                                                                                                                                                                                                                         <  2:397932/251‑2 (MQ=255)            atatTTATCAACAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTATTTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGTCTATATGCCCTGaaa                                                                                                                                                                                                                                     <  1:350500/251‑1 (MQ=255)                    cACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTATTTTTATGTTTGTATGCAGATGAAGCAATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGTCTATATGCCCTGAAACGAGCAAt                                                                                                                                                                                                                             >  2:66513/1‑251 (MQ=255)                            aTAGTACTAAATCTATCGCCTGAATGATCCCCAGAGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCCACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGCGCACTTTATTATTTTTATGTTTGTATGCATATAAAGCCATCTATTCGTGTTTTACTATTTCACATAATAAACTCAAACCCTTTTCCCTGCTGTCTATATTCCCTGAAACGCGCAATGCATaact                                                                                                                                                                                                                     >  2:228792/1‑249 (MQ=255)                                                     gATCCCCAAGGAAGATAAAAGGGGATATCGCATCGCCCTTTAAAAAAACATGTTGAAGAAACTCATTCAAGATCATATCTATCGAATTTAATAAATCGTAAAGCACTTTATTATTTTTATGTTTGTATGAAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACCTAATAAACTCCAACCCTTTTCCCAGCTGTCTCTATGACCAGCAACAAGCACTGCATAAAGTACAGCACCGACTCTTCCATcggt                                                                                                                                                                                             >  2:205255/1‑247 (MQ=255)                                                        ccccAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTATTTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGTCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCg                                                                                                                                                                                         <  2:386985/251‑1 (MQ=255)                                                                 aGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTATTTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGTCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATa                                                                                                                                                                                 >  1:292434/1‑250 (MQ=255)                                                                     aCAAGGGGTTATCGCATCGTCCTTTAATAAAACATGTTGATGCACATCATTCAAAATCATGTCTCTCGTATTTAATAAATCGTCGAGCACTTTATTATTTTTATGTTTGTCTGCAGATGAAGCCATCTCTTCGGGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGCTGTCTATATGCCCTGAAACGAGCCACGCATAAAGAAAAGCGCCTACTCTTCCATCTGCATCACCAAAATAAgact                                                                                                                                                                            >  2:507333/1‑248 (MQ=255)                                                                                             taaaaaaacctgtttaatgaaatCATTTAAGATCATGTCTTTCGGATTTAATAAATCGTAGTGCACTTTATTATTTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGTCTATATGCCCTGAAACGAGCAATTCATATAGTACAGCGCTTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAAt                                                                                                                                                      <  2:468183/227‑1 (MQ=255)                                                                                                          tGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTATTTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGTCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGtt                                                                                                                                        <  1:548553/250‑1 (MQ=255)                                                                                                             tGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTATTTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGTCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTtat                                                                                                                                     >  1:189196/1‑250 (MQ=255)                                                                                                                       tCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTATTTGTAGTTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAATCTCCAACCCTTTTCCCTGATGTCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCTTACGCTTCCATCTGTATCGCCAAAATAAGCCGTGTTTGCATTTTGTATGTTAATCACACCACACTGTTTATAGAATTCTAt                                                                                                                           <  2:558204/250‑1 (MQ=255)                                                                                                                                                   aTCGTAGAGCACTTTATTATTTTTCTGTTTGTATGCAGATGAAGCCATCTCTTAGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTACCTGATGTCTATATGCCCTGAAACGAGCAATGAATAAAGAACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAACAGTGTTGGCATTTTGTGGGTTAATCACACAACACTGTTTATAGAAGTCTATTTTTGACAACACAGATTTAACATAGCTAt                                                                                              >  2:509507/1‑251 (MQ=255)                                                                                                                                                     cGTAGAGCACTTTATTATTTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGTCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGAAAACACAGATTTAACATAGATATc                                                                                             >  1:108489/1‑250 (MQ=255)                                                                                                                                                                                   aTGCAGATGAAGCCATCTTTTTGTTTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGTCTATATGCCCTGAAACTAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGAAAACACAGATTTAACATAGCTATCAAACTTTTCCTTAGTAAACGTCTCTTTaga                                                               <  1:153948/250‑1 (MQ=255)                                                                                                                                                                                                     ctTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGTCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTg                                                                                                                                          <  2:31901/157‑1 (MQ=255)                                                                                                                                                                                                     ctTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGTCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTg                                                                                                                                          >  1:31901/1‑157 (MQ=255)                                                                                                                                                                                                              ttAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGTCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGAAAACACAGATTTAACATAGCTATCAAACTTTTCCTTAGTAAACGTCTCTTTAGAGACATCCCAACGCCAGTCAGGTTTGaa                                    >  1:27776/1‑250 (MQ=255)                                                                                                                                                                                                                           taataaACTCCAACCCTTTTCCCTGATGTCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATcac                                                                                                                                                   >  2:155150/1‑126 (MQ=255)                                                                                                                                                                                                                           taataaACTCCAACCCTTTTCCCTGATGTCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATcac                                                                                                                                                   <  1:155150/126‑1 (MQ=255)                                                                                                                                                                                                                                   tCCAACCCTTTTCCCTGATGTCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCCACTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGCCTATTTTTGAAAACACAGATTTAACATAGCTATCAAACTTTTCCTTAGTAAACGTCTCTTTAGAGACATCCCAACGCCAGTCAGGTTTGAAATTACCACTAAGAAGAGTaa                >  1:361080/1‑249 (MQ=255)                                                                                                                                                                                                                                               cccTGATGTCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACTCTGTTTAGAGAAGTCTATTTTTGAAAACACAGATTTAACATAGCTATCAAACTTTTCCTTAGTAAACGTCTCTTTAGAGACATCCCAACGCCAGTCAGGTTTGAAATTACCACTAAGAAGAGTAAAGAAATTTCTCGCt  <  1:336805/251‑1 (MQ=255)                                                                                                                                                                                                                                               cccTGATGTCTATATGACCTGAAACGAGCAACGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGGTTATAGAAGTCTATTTTTGAAAACACAGATTTAACATAGCTATCAAACTTTTCCTTAGTAAACGTCTCTTTAGAGACATCCCAACGCCAGTCAGGTTTGAAATTACCACTAAGAAGAGTAAAGAAATTTCTCGc   <  1:507333/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   AAAGTAAGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTATTTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGAAAACACAGATTTAACATAGCTATCAAACTTTTCCTTAGTAAACGTCTCTTTAGAGACATCCCAACGCCAGTCAGGTTTGAAATTACCACTAAGAAGAGTAAAGAAATTTCTCGCT  >  NC_000913/3633503‑3633991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑