BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,621,090	C→T	R633R (CGC→CGT)	rhsB →	rhs element protein RhsB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,621,090	0	C	T	98.0%	187.4 / NA	103	R633R (CGC→CGT)	rhsB	rhs element protein RhsB
Reads supporting (aligned to +/- strand): ref base C (0/1); major base T (48/51); minor base A (1/1); total (50/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

TTATGACCATGACCGCTTCGGGCAGATGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTACCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGCAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATCACCACGACCTGACCGGCAAACTTATCCGCAGCGAGGATGAGGGTCTGGTCACCCACTGGC‑ACTATGACGAAGCAGA‑CCGCCTCACGCACCGCACCGTGAAGGGTGAAAC  >  NC_000913/3620844‑3621332

ttATGACCATGACCGCTTCGGGCAGATGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCCGTACCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGGCATTTCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGGTGCGTGGGGGAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGAAGt                                                                                                                                                                                                                                                      >  2:286523/1‑250 (MQ=255)

  atgaccatgaccGCTTCGGGCAGATGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTACCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATg                                                                                                                                                                                                                                                   >  1:440463/1‑251 (MQ=14)

  atgaccatgaccGCTTCGGGCAGATGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTACCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATg                                                                                                                                                                                                                                                   <  1:308648/251‑1 (MQ=14)

           acagCTTCGGGCAGATGACGACGGTGCACCGCGGGGAAGGGCTGAGTCAGTAGCGCGAATACGACAGCCGTGGACAGTTAATTGCCGTGCAAGAAACGCAGGGCCATGAACCGCGGTATGAATACAACATCGCCGGTGACCTG‑CCCGCCGTCATTGCCACGGACGGCAGCAGAAACGGGGAC‑CAGTC‑CGATGCGTGGGGCAAGGCCGTCCGTACCACGACGGGCGGGCTG‑ACGAGTAGGATGGaaaaagac                                                                                                                                                                                                                                          >  2:264397/4‑245 (MQ=11)

            ccGCTTCGGGCAGATGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTACCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGAtg                                                                                                                                                                                                                                         >  1:509855/1‑251 (MQ=14)

             cGCTCCGGGAAGATGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTACCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTGTGGAATACGAtgc                                                                                                                                                                                                                                        <  1:255777/251‑1 (MQ=9)

              gctgcGGGCAGATGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGGACCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACATCATCGCCGGTGACCGG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGGACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGAtgct                                                                                                                                                                                                                                       <  1:555034/247‑1 (MQ=255)

                   gggCAGATGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTACCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCgg                                                                                                                                                                                                                                  >  1:136048/1‑251 (MQ=14)

                   gggCAGATGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTACCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCgg                                                                                                                                                                                                                                  >  1:73302/1‑251 (MQ=14)

                     gCAGATGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTACCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGGAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGAc                                                                                                                                                                                                                                >  1:358835/1‑251 (MQ=14)

                        gATGACGGCGGGTCACCGCGAGGAAGGGCTGATTCAGTACCGCGCATACGACAGCCGTGGACAGTTAATTTCCGTGAAAGACACGGAGGTCCATGAAACGCGGTATGAATACAACATCGCCGGTGGCCTG‑ACCGCCGTCAGTGCCGCGGACGGCAGCAGAAACGGG‑ACAGAGTA‑CGAGGGGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTAGGGAATACGATGCTGCCGGACggg                                                                                                                                                                                                                             <  1:516201/251‑1 (MQ=11)

                           gACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTACCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTc                                                                                                                                                                                                                           <  1:7563/250‑1 (MQ=14)

                            aCGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTACCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCAt                                                                                                                                                                                                                         <  1:261410/251‑1 (MQ=14)

                                 ggTGCACCGCGAGGAAGGGCTGAGTCAGTACCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGCCGGGTCATCCGcc                                                                                                                                                                                                                    >  1:96641/1‑251 (MQ=14)

                                 ggTGCACCGCGAGGAAGGGCTGAGTCAGTACCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGcc                                                                                                                                                                                                                    >  1:380561/1‑251 (MQ=14)

                                    gCACCGGGAGGAAGGGCTGAGTCAGTACCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTg                                                                                                                                                                                                                  <  1:212786/250‑1 (MQ=14)

                                      aCCGCGAGGAAGGGCTGCGTCAGTACCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCCGCAGAAACGGG‑ACCCAGTA‑CGATGCGGGGGGAAAGGCCGTCCGTACCACGCAAGGCGGGCAG‑GCCGGGAGTTGGGAATAAGAAGCTGCCGGACGGGTCATCCGCCTgccc                                                                                                                                                                                                               >  2:217882/1‑248 (MQ=11)

                                       ccGCGAGGAAGGGCTGATTCAGTACCGCGCATACGACAGCCTTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCa                                                                                                                                                                                                              <  1:527608/251‑1 (MQ=14)

                                                  gtgtgagtcagctccgggataaCGACAGCCGGGGACAGGTAATGGCCGTGAAAGACGCGCAGGGCCATGAAACGGGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGc                                                                                                                                                                                                   <  2:222742/230‑1 (MQ=11)

                                                     ggagacaggaacgcgcagccGACAGCCGGGGACAGTTAATGGCCGTGAAGGACACGAAGGGCCAAGAAACGGGGTAGGAATAAAACATCGCCGGTGACCGG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGc                                                                                                                                                                                                <  2:224080/232‑1 (MQ=11)

                                                      gAGTCAGGACCGCGCATACGACAGCCGTGGAAAGTTAATTGCCGCGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGcc                                                                                                                                                                                               <  2:303371/251‑1 (MQ=9)

                                                       aGTCAGTACCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGcc                                                                                                                                                                                               >  1:159357/1‑250 (MQ=14)

                                                           aatACCGCGCATCCGACAGCCGTGGACAGTTAAGTGCCGTGAAAGACACGCAGTGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑CCCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCAcacc                                                                                                                                                                                          <  1:68243/249‑1 (MQ=255)

                                                             tACCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGCAAACGGCAGCCAcacca‑c                                                                                                                                                                                       >  1:28126/1‑251 (MQ=14)

                                                              aCCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCAcacca‑c                                                                                                                                                                                       <  1:500737/250‑1 (MQ=14)

                                                                cgcgCATACGACAGCCGTGGACAGTAAATGGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCtt                                                                                                                                                                                    <  1:8814/251‑1 (MQ=14)

                                                                 gcgcATACGACAGCCGTGGAAAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTc                                                                                                                                                                                   <  1:362834/251‑1 (MQ=14)

                                                                  cgcATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCCGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTcc                                                                                                                                                                                  >  1:337280/1‑251 (MQ=14)

                                                                  cgcATACGACAGCCGTGGACAGTTAATTGCCGTAAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTcc                                                                                                                                                                                  <  1:93665/251‑1 (MQ=14)

                                                                     aTACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGAtgct                                                                                                                                                                                                                                       >  1:463087/1‑196 (MQ=17)

                                                                     aTACGACAGCCGTGGACAGTTAATTGCCGTGAAAACCACGAAGGGCCATGAAACGCGGAATGAATACAACAACGCCGTTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGGCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGtt                                                                                                                                                                               <  2:284204/251‑1 (MQ=11)

                                                                                    aCAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGAcc                                                                                                                                                               >  1:160913/1‑251 (MQ=14)

                                                                                       gTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAAGACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGc                                                                                                                                                            <  1:231382/251‑1 (MQ=14)

                                                                                         tAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCGTCCGTTACGATG‑TACTTGACCGGCt                                                                                                                                                           <  1:153809/250‑1 (MQ=14)

                                                                                          aaTTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTg                                                                                                                                                          <  1:316809/250‑1 (MQ=14)

                                                                                           aTTGCTGTAAAAGACACCAAGGGCCAGGAAACGGGGTATGAATAAAACATCGCCGGGGCCCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑t                                                                                                                                                       <  2:233124/251‑1 (MQ=11)

                                                                                           aTTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGAAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTACCGTTACGATG‑TACTTGACCGGCTGA‑t                                                                                                                                                       >  1:238494/1‑251 (MQ=14)

                                                                                              gCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TAc                                                                                                                                                     >  1:60526/1‑250 (MQ=14)

                                                                                               ccGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAg                                                                                                                                                   <  1:402920/251‑1 (MQ=14)

                                                                                                cggaaaacacacGCAGGGCATTGAAACGCGTTATGAAAACAACAGCGCGGGGCTCCTG‑CCCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGTGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGATCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAgg                                                                                                                                                  <  2:300920/243‑1 (MQ=11)

                                                                                                 gTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAgg                                                                                                                                                  >  1:280209/1‑250 (MQ=14)

                                                                                                     aaGACACGCAGGTCCCTGAAACGCGGTATGAATACAACATCGCAGGTGACCTG‑CCCGCCGTCATCGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGACAGGCCGTCCGACCCACGCAGCGAGAGATG‑ACCCGTAATATGGCAGACGATGCTGCCACCAGGGGCATCCGCGTGACCCGAGCAAACGGCAGC‑ACACCAACCCTCCCTTAAGCTG‑TATTCGACCGGCTgcgactagcaaac                                                                                                                                              >  2:324448/1‑239 (MQ=2)

                                                                                                      aGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCg                                                                                                                                            >  1:271864/1‑251 (MQ=14)

                                                                                                       gACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCgg                                                                                                                                           <  1:260533/251‑1 (MQ=14)

                                                                                                         cacGCAGGGCCATGAAACGCGGTCTGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGAAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGAGCTG‑ACGCGTAGTATGGAATACGATTCTGCCGGACGGGTCCTCCGCCTGACCAGGGAAAACGGCAGCCACACCA‑CCTCCCGTTACGATG‑AACTTAACCGGCtagaaagggaaaacggct                                                                                                                                          >  2:441280/1‑234 (MQ=11)

                                                                                                          ccGCAGGGCCATGAACCGCGGTATGAATACAACATCGCCGGGGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCtt                                                                                                                                        <  1:264397/250‑1 (MQ=9)

                                                                                                          acGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCtt                                                                                                                                        >  1:107066/1‑251 (MQ=14)

                                                                                                                 gCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGc                                                                                                                                 >  1:200459/1‑251 (MQ=14)

                                                                                                                  ccaggaaactgggTAGGAATACAACACCGCCGGTTGCCCG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTTACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGcc                                                                                                                                <  2:318222/240‑1 (MQ=11)

                                                                                                                  ccATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGCAAACGGCAGCCACACCA‑CCGTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGcc                                                                                                                                >  1:499287/1‑251 (MQ=14)

                                                                                                                   cATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCTGCTGA‑TACAGGAAACCg                                                                                                                                            <  1:450989/238‑1 (MQ=14)

                                                                                                                   cATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCg                                                                                                                               <  1:55449/251‑1 (MQ=14)

                                                                                                                   cATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTACCGATG‑TACTTGACCGGCTGA‑TACAGGAAACCgcgaacggaagggg                                                                                                                               >  2:450989/1‑238 (MQ=255)

                                                                                                                    aTGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGaaaa                                                                                                                                                                                                       <  1:234612/181‑1 (MQ=17)

                                                                                                                    aTGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGaaaa                                                                                                                                                                                                       >  2:234612/1‑181 (MQ=17)

                                                                                                                      gAAACGCGTTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGca                                                                                                                             <  1:297486/250‑1 (MQ=14)

                                                                                                                            cgggaTGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGCTGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGACGCCACACCA‑CCTTCCGTTACGGTGGTA‑TTGACCGGCTTAGTA‑AGGGAACCGGCTTTggcagccccccaccacg                                                                                                                      >  2:166280/5‑235 (MQ=11)

                                                                                                                            cgGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCg                                                                                                                      <  1:487731/251‑1 (MQ=14)

                                                                                                                              gttggatgacaacaTCGCCGGGGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑CCACAGTA‑CGTGGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCGGACCAGTGAAAACGGCAGCCACACCT‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCGtt                                                                                                                    <  2:463085/243‑1 (MQ=11)

                                                                                                                                      caatcaccGCCGGTGAC‑TGCACCTCCGTCATGTCCCCTGACGGGCGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCGGTCCGTACCACGAAGGGCGGGCTG‑ACGTGTAGTATGGAATACGATGTTGCCGGACGGGTCTTCCGCCTTACCAGTGAAATCGGCAGCCTCACCA‑CCTGCCGTTACGATG‑TACTTGACCGGCTGC‑TACAGTAAACCGGCTTTGACGGCCGCACACAGCTTTATcaccac                                                                                                            <  2:522413/244‑1 (MQ=11)

                                                                                                                                      acaacaTCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATcaccac                                                                                                            <  1:242672/251‑1 (MQ=14)

                                                                                                                                        aacaTCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGATTTGACGGCCGCCCACAGCGTTATCACCACgc                                                                                                          >  1:280021/1‑250 (MQ=9)

                                                                                                                                          caTCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACCGCGTTATCACCACGAcc                                                                                                        >  1:232979/1‑251 (MQ=14)

                                                                                                                                               ccGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATCACCACGACCTGAcc                                                                                                   >  1:393510/1‑251 (MQ=14)

                                                                                                                                                    gacctg‑accGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATCACCACGACCTGACCGGCa                                                                                               <  1:422075/250‑1 (MQ=14)

                                                                                                                                                      cctg‑accGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATCACCACGACCTGACCGGCAAAc                                                                                            >  1:125941/1‑251 (MQ=14)

                                                                                                                                                         g‑accGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATCACCACGACCTGACCGGCAAACtt                                                                                          >  1:463474/1‑250 (MQ=14)

                                                                                                                                                         g‑accGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATCACCACGACCTGACCGGCAAACTTa                                                                                         <  1:130635/251‑1 (MQ=14)

                                                                                                                                                                cgTCATTGCCCCGGCCGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATCACCACGACCTGACCGGCAAACTTATCCGcc                                                                                   >  1:424405/1‑250 (MQ=14)

                                                                                                                                                                cgTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGACAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCATGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGCTG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATCACCCCGACCTGACCGGCAAACTTaacagc                                                                                    >  1:572270/1‑245 (MQ=11)

                                                                                                                                                                cgTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATCACCACGACCTGACCGGCAAACTTATCCGc                                                                                    >  1:192936/1‑250 (MQ=14)

                                                                                                                                                                        ccccGGACGGCAGCGGAAACGGG‑‑GACAATATCGATGCGTGGGGAAAGGCCGGTGGGACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCGGCCGGACGTGTCATCCGCCTGACCCGTGAAAACGGCAGCCACACCA‑CCTTCCGTTCCGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATCACCACGACCTGACCGGCAAACTTATCCGCAGCGAGGAt                                                                           <  2:140143/251‑1 (MQ=11)

                                                                                                                                                                          ccGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGAGGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATCACCACGACCTGACCGGCAAACTTATCCGCAGCGAGGATGa                                                                         <  1:142430/251‑1 (MQ=14)

                                                                                                                                                                           cGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATCACCACGACCTGACCGGCCAACTTATCCGCAGCGAGGATg                                                                          >  1:4707/1‑249 (MQ=14)

                                                                                                                                                                                     cagAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACAAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATCACCACGACCTGACCGGCAAACTTATCCGCAGCGAGGATGAGGGTCGGGTCa                                                              <  1:109883/251‑1 (MQ=14)

                                                                                                                                                                                           cGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGCAACCGGCTTTGACGGCAGCACACAGCGTTATCCCCACGACCTGCCCGGCAAACTTATCCCCCGCGAGGCTGCGGGTCTGGTCAccccac                                                        >  2:325403/1‑248 (MQ=11)

                                                                                                                                                                                            ggg‑ACCCAGTA‑CGAGGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAAGACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATCACCACGACCTGACCGGCAAACTTATCCGCAGCGAGGATGAGGGTCTGGTCACCCACTg                                                       <  2:73302/251‑1 (MQ=9)

                                                                                                                                                                                             gg‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATCACCACGACCTGACCGGCAAACTTATCCGCAGCGAGGATGAGGGTCTGGTCACCCACTgg                                                      <  2:192936/251‑1 (MQ=14)

                                                                                                                                                                                                  ccaGTA‑CGATGCGGTGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATCACCACGACCTGACCGGCAAACTTATCCGCAGCGAGGATGATGGTCTGGTCACCCACTGGC‑ACt                                                 <  2:380561/250‑1 (MQ=9)

                                                                                                                                                                                                   caGTA‑CGATGCGTGGGGAAAGGCCGGCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATCACCACGACCTGACCGGCAAACTTATCCGCAGCGAGGATGAGGGTCTGGTCACCCACTGGC‑ACTa                                                <  1:214966/251‑1 (MQ=14)

                                                                                                                                                                                                     gTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATCACCACGACCTGACCGGCAAACTTATCCGCAGCGAGGATGAGGGTCTGGTCACCCACTGGC‑ACTATg                                              <  1:286523/251‑1 (MQ=14)

                                                                                                                                                                                                      gtctgATGCGTGGTGAAAGGCCGGCCGTGCCACGCAGGGCGGG‑TGTACGCGAATTATGGAATACGTTGCTGCCGGACGTGTCATCCGCCTGACCAGTGCAAACGGCGGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCATTCTCACCACGACCTGACCGGCAAACTTAGCCGCAGCGAGGATGAGGGTCTGTTCACTCACGGGC‑TCTATg                                              <  2:527690/247‑1 (MQ=11)

                                                                                                                                                                                                         cGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATCACCACGACCTGACCGGCAAACTTATCCGCAGCGAGGATGAGGGTCTGGTCACCCACTGGC‑ACTATGACg                                           >  1:511398/1‑251 (MQ=14)

                                                                                                                                                                                                           aTGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATCACCACGACCTGACCGGCAAACTTATCCGCAGCGAGGATGAGGGTCTGGTCACCCTCTGGC‑ACTATGACGaa                                         <  1:395838/251‑1 (MQ=14)

                                                                                                                                                                                                                   ggAAAGGCCGTCCGTAAAACGCAGGGCGGGCTG‑ACGCGTAGGATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TCCTTGACCGGCTGA‑TAAAGGAAACCGGCTTTGACGGCCGCACACAGCGGTATAACCACGACCTGACCGGAAAACTATTCCGCAGAGAGGGAGAGGGGCAGGACACCCACTGGCGAC‑ATGACGAAGAAGA‑cac                                >  2:18463/1‑249 (MQ=11)

                                                                                                                                                                                                                    gAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATCACCACGACCTGACCGGCAAACTTATCCGCAGCGAGGATGAGGGTCTGGTCACCCACTGGC‑ACTATGACGAAGCAGA‑CCGc                               >  1:292187/1‑251 (MQ=14)

                                                                                                                                                                                                                        ggCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TCCTTGACCGGCTGA‑TACAGGAACCCGGCTTTGACGGCCGCACACAGCGTTATCACCACGACCTGACCGGCAAACTTACCCGCAGCGGGGGTGAGGGTCTGGCCCCCCACTGGC‑CCTACGCCGAAGCAGaacaccgc                             >  2:5450/1‑243 (MQ=11)

                                                                                                                                                                                                                         gccggccgTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTGTGACGGCCGCACACAGCGTTATCCCCACGACCTGACCGGCAAACTTATCCGCAGCGAGGATGAGGGTCTGGTCACCCACTGGC‑ACTATGACTAAGCAGA‑CCGCCTCAc                          <  2:125941/251‑1 (MQ=9)

                                                                                                                                                                                                                         gCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATCACCACGACCTGACCGGCAAACTTATCCGCAGCGAGGATGAGGGTCTGGTCACCCACTGGC‑ACTATGACGAAGCAGA‑CCGCCTCAc                          >  1:103263/1‑251 (MQ=14)

                                                                                                                                                                                                                         gCCGTCAGTACCACGCAGGGCGGGCTG‑CCGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCACACACAGCGTTATCAACAGGACCTGAACGGCTAACTTTACCGCAGCGAGGACGAGAGTCTGGTCACCCCCTGGC‑ACTACGACGAAG‑AGAACCGCCTccc                          >  2:525051/1‑249 (MQ=11)

                                                                                                                                                                                                                          ccgtccgtACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATCACCACGACCTGACCGGCAAACTTATCCGCAGCGAGGATGAGGGTCTGGTCACCCACTGGC‑ACTATGACGAAGCAGA‑CCGCCTCAcg                         <  1:164265/251‑1 (MQ=14)

                                                                                                                                                                                                                             cccgtgtcaccaggggctggcTG‑TCTCGCAGTATGGAAGACGCTGCGGCCGCACGTGTCAGCCGCCTGTCCAGTGAAATGGGCAGCCACATCA‑CCTGCCGTTACGATG‑TACGTGACCGGCTGA‑CACAGGAAACCGGTATTGACGGCCGCACGCAGCTTTATCTCCACGACCGGACCGGCAAACTTATCCGCATCGAGGATGAGTTCCTGGGCGCCAACTGGC‑ACTATGACGAAGCAGA‑CCGCCTCAcgcac                      <  2:60284/238‑1 (MQ=2)

                                                                                                                                                                                                                              ccgtACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATCACCACGACCTGACCGGCAAACTTATCCGCAGCGAGGATGAGGGTCTGGTCACCCACTGGC‑ACTATGACGAAGCAGA‑CCGCCTCAcgcacc                     >  1:307854/1‑251 (MQ=14)

                                                                                                                                                                                                                                gtACCACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATCACCACGACCTGACCGGCAAACTTATCCGCAGCGAGGATGAGGGTCTGGTCACCCACTGGC‑ACTATGACGAAGCAGA‑CCGCCTCAcgcaccgc                   >  1:255383/1‑251 (MQ=14)

                                                                                                                                                                                                                                    cACGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATCACCACGACCTGACCGGCAAACTTATCCGCAGCGAGGATGAGGGTCTGGTCACCCACTGGC‑ACTATGACGAAGCAGA‑CCGCCTCACGcaccgcaccg               >  1:520286/1‑251 (MQ=14)

                                                                                                                                                                                                                                      cGCAGGGCGGGCTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATCACCACGACCTGACCGGCAAACTTATCCGCAGCGAGGATGAGGGTCTGGTCACCCACTGGC‑ACTATGACGAAGCAGA‑CCGCCTCACGCACCGCACCGTg             <  1:283555/251‑1 (MQ=14)

                                                                                                                                                                                                                                          gggcgggcTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATCACCACGACCTGACCGGCAAACTTATCCGCAGCGAGGATGAGGGTCTGGTCACCCACTGGC‑ACTATGACGAAGCAGA‑CCGCCTCACGCACCGCACCGTGAAgg         <  1:511445/251‑1 (MQ=14)

                                                                                                                                                                                                                                              gggcTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATCACCACGACCTGACCGGCAAACTTATCCGCAGCGAGGATGAGGGTCTGGGCACCCACTGGC‑ACTATGACGAAGCAGA‑CCGCCCCACCCCCCGCACCGGGAAGGGTg      >  2:422637/1‑250 (MQ=255)

                                                                                                                                                                                                                                                gcTG‑ACGCGTAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATCACCACGACCTGACCGGCAAACTTATCCGCAGCGAGGATGAGGGTCTGGTCACCCACTGGC‑ACTATGACGAAGCAGA‑CCGCCTCACGCACCGCACCGTGAAGGGTGaaa   <  1:148911/251‑1 (MQ=14)

                                                                                                                                                                                                                                                   g‑ACGCGTAGTATGGAATACGAGGCGGCCGGACGGGTCATACGCCTGACCAGTGAAAACGGCAGCCACACCC‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TCCAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATCACCACGACCTGACCGGCAAACTTATCCGCAGCGAGGATGAGGGTCTGGTCACCCACTGGC‑ACTATGACGAAGCAGA‑CTGCCTCACGCACCGCACCGTGAAGGGTGAAAc  <  2:136048/249‑1 (MQ=11)

TTATGACCATGACCGCTTCGGGCAGATGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTACCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTG‑ACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGG‑ACACAGTA‑CGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTG‑ACGCGCAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCA‑CCTTCCGTTACGATG‑TACTTGACCGGCTGA‑TACAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATCACCACGACCTGACCGGCAAACTTATCCGCAGCGAGGATGAGGGTCTGGTCACCCACTGGC‑ACTATGACGAAGCAGA‑CCGCCTCACGCACCGCACCGTGAAGGGTGAAAC  >  NC_000913/3620844‑3621332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`