BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,589,956	G→A	L24L (CTC→CTT)	ugpE ←	sn‑glycerol 3‑phosphate ABC transporter membrane subunit UgpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,589,956	0	G	A	100.0%	370.0 / NA	115	L24L (CTC→CTT)	ugpE	sn‑glycerol 3‑phosphate ABC transporter membrane subunit UgpE
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (58/57); total (58/57)

Only 100 of 115 total aligned reads displayed.

CGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCA‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTG  >  NC_000913/3589711‑3590201

agAAACCAGACAATGGCAAATGCCGAGAGCAGCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCA‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGAt                                                                                                                                                                                                                                                  <  2:375731/250‑1 (MQ=255)

         aCAATTGCCAATGCCGAGAGAATCGAGACTGTATGTTTGCCGAGGGTAATGCTGAACGCCATCACAAAGCGGTTAAGCAACAACCGCCA‑GACCGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTGTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCATGTTTATCCAGCGTCGTCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGAt                                                                                                                                                                                                                                         <  2:524099/251‑1 (MQ=255)

         aCAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCA‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGAt                                                                                                                                                                                                                                         <  1:113661/251‑1 (MQ=255)

            actgCAAATGCCGAGAGCATCGTGACGGTAAGGTTGCCGAGCGTAATGCAGAACGCCATCTCAACGCTGGTAAGCAACATCCGCCA‑GAACGGCGCGCTATTCGTGCCTACCCAGTTCAACCAGAGGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGTCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATctc                                                                                                                                                                                                                                      <  2:182034/248‑3 (MQ=255)

             tGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCA‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCg                                                                                                                                                                                                                                     >  1:132263/1‑251 (MQ=255)

                 aaaTGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCA‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTACAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGGCGCCGCGCCA‑AACGCCACGTACAGAGGGAAAAGGAACACAGCGGTCCCggggg                                                                                                                                                                                                                                 >  2:450316/1‑247 (MQ=255)

                   aTGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCA‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATc                                                                                                                                                                                                                               >  1:414329/1‑251 (MQ=255)

                   aTGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCA‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATc                                                                                                                                                                                                                               >  1:242202/1‑251 (MQ=255)

                      ccGAGGGCATCGAAACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCA‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCGCCCAGATGTTGTGGATGTTTTCCAGCAGATGTGGGCCGGGGATGAGCGTCATCGGCGCGGTATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGTAAAAGGATCACCGCGATCCCGAGGATCAGc                                                                                                                                                                                                                            <  2:240000/251‑1 (MQ=255)

                       cGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCA‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGc                                                                                                                                                                                                                            >  1:116108/1‑250 (MQ=255)

                            gCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCA‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTa                                                                                                                                                                                                                      <  1:566581/251‑1 (MQ=255)

                               acGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCA‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATgg                                                                                                                                                                                                                   >  1:370464/2‑251 (MQ=255)

                                 gagaCGGTAATTCTGCCGAGCGTAATGCTGAACGCCATCACCACGCTGTTAAGCAACATCCGCCA‑GAACGGCGCGCTATTCGTGCCTCCCCCGTTCACCCAGATGATGTGGATGTTTTCCAACCGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGAATAGACGGACTGTTTATCCAGCGTCGCCGCG‑CACAACGCAACGTACCGCGGTAAAAGGATCACCCCGCTCCCGCGGagagccacggtatggcg                                                                                                                                                                                                                 >  2:535014/1‑235 (MQ=255)

                                     cGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCA‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGAGGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAat                                                                                                                                                                                                             <  1:88815/251‑1 (MQ=255)

                                            tttGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCA‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGCACAGCGGGCAAAGGATCACCGCGATCCCGGGGAACAGCAGGGTAGGTCTGGATATcaca                                                                                                                                                                                                       >  2:458859/1‑247 (MQ=255)

                                             ttGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCA‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCAGAGACGGCCGGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCa                                                                                                                                                                                                      <  1:520695/250‑1 (MQ=255)

                                                 cGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCA‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCac                                                                                                                                                                                                 <  1:324747/251‑1 (MQ=255)

                                                    gCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCA‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCacgg                                                                                                                                                                                               >  1:41465/1‑250 (MQ=255)

                                                     cGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCA‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCacggac                                                                                                                                                                                             <  1:165204/251‑1 (MQ=255)

                                                      gTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCA‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCacggacg                                                                                                                                                                                            <  1:538834/251‑1 (MQ=255)

                                                       tAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCA‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACC‑AACGCCACGTACAGCGGGAAAAGGATCACCGAGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCacggacgg                                                                                                                                                                                           >  2:369981/1‑251 (MQ=255)

                                                       tAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCA‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGCTCCCGAGGTTCAGCATGGTATGGCTGAATATCGTCAGCCacggacgg                                                                                                                                                                                           >  1:20324/1‑251 (MQ=255)

                                                         aTGCTGACCGCCATCACATACCTGGTAAGCAACTTCCGCTA‑GAACGGCGCGCTATTCGTGCCCACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCCGATGTGTCCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATACAGCGTCGCCGCCACA‑ACCGCCACGTACCGCGGGAAAAGGATCACCGCGCTCCCGGGGACCAGCATGGGATGGCAGAatctggccatccaaggctgggt                                                                                                                                                                                         >  2:48139/1‑231 (MQ=255)

                                                             tGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCA‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGCTGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGACGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGCTCCAGAGGATCAGCATGGAATGGCTGAATATCGTCAGCACCGGGCGGTtctc                                                                                                                                                                                      >  2:264207/1‑250 (MQ=255)

                                                                   ccATCACAAAGCTGTTAAGCAACATCCGCCA‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGGCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCAtt                                                                                                                                                                               <  1:217415/251‑1 (MQ=255)

                                                                    cATCACAAAGCTGTTAAGCAACATCCGCCA‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTg                                                                                                                                                                              >  1:54512/1‑251 (MQ=255)

                                                                       cacaAAGCTGTTAAGCAACATCCGCCA‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGt                                                                                                                                                                            >  2:144584/1‑250 (MQ=255)

                                                                       cacaAAGCTGTTAAGCAACATCCGCCA‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTa                                                                                                                                                                           <  1:94036/251‑1 (MQ=255)

                                                                         caAAGCTGTTAAGCAACATCCGCCA‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAAc                                                                                                                                                                         <  1:288270/251‑1 (MQ=255)

                                                                                gatAAGCAATATCGGCCA‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGGTTTCCAGCAGATGCGTGCCGGGGTTGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGACGCGTCA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCAGGAGGATCACCATGGAATGGCTGAATATCGTCAGCCACGCAAGGTTCTGAACCATTGGTAACACACCtt                                                                                                                                                                  >  2:203031/3‑251 (MQ=255)

                                                                                gTTAAGAAACATCC‑CCATGAACGGTGCGCTATGCGTGCTTACCCCGTTCACCCAGATGGTGTGGATGTGTGCCAGCTGATGTGTGCCGGGGATTAGCGTAATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATGGGTAACGCACCt                                                                                                                                                                   <  2:7914/250‑1 (MQ=255)

                                                                                 ttAAGCAACATCCGCCA‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCCCGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTAAGGCTGAATATCGTCAGCCACGGCCGGTTCTCAAACATTGGTAACGCACCTTg                                                                                                                                                                 >  2:327058/1‑251 (MQ=255)

                                                                                 gtAAGCAACATCCGCCA‑GAAAGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTg                                                                                                                                                                 <  2:163648/250‑1 (MQ=255)

                                                                                    aGCAACATCCGCCA‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCTGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCtt                                                                                                                                                              <  1:345568/251‑1 (MQ=255)

                                                                                        aCATCAGCCA‑GAACGACGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGAGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGACTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGCATGGCTGAATATCGTCAGCCACGACCGGGTCTCAAGCATTGGTAACGCACATTGCTTTCa                                                                                                                                                           >  2:189809/1‑250 (MQ=255)

                                                                                         cATCCGCCA‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGc                                                                                                                                                                <  1:279295/244‑1 (MQ=255)

                                                                                          aTCCGCCA‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACa                                                                                                                                                        <  1:450316/251‑1 (MQ=255)

                                                                                              gCCA‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGc                                                                                                                                                    <  1:429597/251‑1 (MQ=255)

                                                                                               ccA‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCg                                                                                                                                                   <  1:26717/251‑1 (MQ=255)

                                                                                                 acatgagtctcgttATTCGTGCCGGCCCCGTTCACCCAGTTGTTATGGGTGTTTTCTAGGAGATGTGTGCCGGGGTTGAGCGTTATCGGCGCTGCATAGACGGCATGTGTATCCAGCGTCGCCGCGACA‑AACGCCCCGTACAGTGGGAAAAGGATCGCCGCGATCCCGAGGATCAGCATGGTATGGCTGTATATCGTCAGCCACGGATGGTTCTCAATCATTGGTAACGCACCTTGCTGTCAACATAGCgg                                                                                                                                                  <  2:376043/238‑1 (MQ=255)

                                                                                                 a‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGa                                                                                                                                                 <  1:12277/251‑1 (MQ=255)

                                                                                                   gAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGGATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACCTAGCggg                                                                                                                                                 >  2:545239/1‑249 (MQ=255)

                                                                                                      cGGCGCGCTATTCGTGCCTACCCCGTTAACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGACGCGACA‑AACGCCACGGACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCAGGGAATGGCTGAATATAGTCAGCCCCGGACGGTTCTCAATCATTGGGAACGCCCCGTGGTTTCAACATCACGGAACTg                                                                                                                                             >  2:139903/1‑251 (MQ=255)

                                                                                                       ggCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCacgg                                                                                                                                                                                               <  1:270387/200‑1 (MQ=255)

                                                                                                       ggCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCacgg                                                                                                                                                                                               >  2:270387/1‑200 (MQ=255)

                                                                                                          gcgcTATTCGTGCCTACCCCGTTCAACCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑CACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCAACGGACGGGTCTCACTCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGcacc                                                                                                                                         >  2:421697/1‑251 (MQ=255)

                                                                                                              tcttCGTGCCTACCCCGTTCACCCAGCTGTTGTGGATGATTTCCAGCAGATGTGTGCCGGGGATGCGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCATCGTCGCCGAGGCA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCCTGGTATGGCTGCATATCGTCAGCCACGGACGGATCTCAATCATTGGAAAAGCAACTTGCTTTCAACATAGAGTAACTTCaccaccc                                                                                                                                     >  2:348954/3‑250 (MQ=255)

                                                                                                               aTTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGt                                                                                                                                    <  1:327058/251‑1 (MQ=255)

                                                                                                               aTTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTCGCTTTCAACATAGCGGAACTGCaccacc                                                                                                                                      <  1:230341/249‑1 (MQ=255)

                                                                                                                  cGTGCCTCCCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCa                                                                                                                                  <  2:4669/250‑1 (MQ=255)

                                                                                                                  cGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCa                                                                                                                                  <  1:325761/250‑1 (MQ=255)

                                                                                                                   gTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGc                                                                                                                                >  1:275629/1‑251 (MQ=255)

                                                                                                                    tGCCTACCCCGTTCACCAAGAAGATGTGAATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCATCATCGGCGAAGCATAGAAGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAACGGGACAAGGATCACCGCGATACCGAGGAACACCATGGTAAGGCTGAATATCGTCAACCACGGCCGATTCTAACTCATTGGTACAGCAACTTGCTTTACACATACAGGGACTGCACCACCGTCAGc                                                                                                                                >  2:554567/1‑250 (MQ=255)

                                                                                                                     gCCTACCCCGTTCACCCAGATTTTGTGGATGTTTTCCAGCGGATGTGTGCCGGGGATGAGCGTCATGGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGGCGCGACA‑AACGCCCCGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTGCAACATAGCGGAACTGCACCACCGTCAGCAc                                                                                                                              <  2:571214/251‑1 (MQ=255)

                                                                                                                          ccccGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATg                                                                                                                          >  1:348097/1‑250 (MQ=255)

                                                                                                                              gTTCACCCAGATGTTGAGGCTGTTTTCCAGCAGCTGCGCGCCGGGGATGAGCGTCATCGGCGCGGCATACCCGGCCTGTTTATCCAGCGCCGCCGCGACA‑CACGCCACCTACCGCGGGAAAAGGCTCACCGCGATCCCGAGGCTCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTACCGCACCTTGCTTTCACCCTACGGGAACTCCAACACCGTCAGCACGCTGACg                                                                                                                       >  2:254870/1‑249 (MQ=255)

                                                                                                                                tCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGCCGGCCTGTTTATACAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGATGATCAGCATGGTATGGCTGGATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGAACCACCCACAGCACGATGCCGa                                                                                                                      >  2:267826/1‑248 (MQ=255)

                                                                                                                                 cACCCAGATGTTGTGGATGTGTTCCAGCTGATGTGTGCCGGGGATGAGCGGCAACGGCGCGGCATAGACGGCCTGTTTATCCAGCGGCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCTCGATGACGAGGa                                                                                                                   <  2:414329/250‑1 (MQ=255)

                                                                                                                                  aCCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGGACAGCGGGAAAAGGATCACCGCGATCCCGAGGATAAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAACGGAACTGCACCACCGTCAGCACGATGCAGAGGAAc                                                                                                                 >  2:48039/1‑251 (MQ=255)

                                                                                                                                   cccAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACa                                                                                                                <  1:144584/251‑1 (MQ=255)

                                                                                                                                    ccAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACAt                                                                                                               >  1:513359/1‑251 (MQ=255)

                                                                                                                                     cAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCA‑TCATTGGTAACGCACCTTGCTTTCAAAATAGCGGGACTGCACCACCGTCAGCACGATGACGAgggacctc                                                                                                              >  1:126973/1‑244 (MQ=255)

                                                                                                                                      aGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCATCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATc                                                                                                              >  1:230886/1‑250 (MQ=255)

                                                                                                                                          gttgtGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAacac                                                                                                         <  1:353127/251‑1 (MQ=255)

                                                                                                                                               ggATGTTTTCCAGCAGCTGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCg                                                                                                    >  1:419931/1‑251 (MQ=255)

                                                                                                                                                 attttttCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCTTCAGCACGATGACGAGGAACATCAacacaacagac                                                                                                  >  2:568904/4‑244 (MQ=255)

                                                                                                                                                   gTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCCCGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTTCCCCACCGTCAGCCCGATGACGAGGACCATCACAACCACCgcacg                                                                                                >  2:513950/1‑247 (MQ=255)

                                                                                                                                                           gcagATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAg                                                                                        <  1:254870/251‑1 (MQ=255)

                                                                                                                                                             agaggtgtgCGGGGGTGGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTTTCCAGCGTGGCCGCGACA‑ACCGCACCGTACAGCGGGAAAATGACCACCGCGAGCCCGAGGAGCAGCATGGTATGGCTGAAGATCGTCAGCTACGGACGTTTCTCAATCATTGGTAACGCCCCTTTCTTTCAACATAGCGGAACTGCCCCCCCGTCAGCACGATGATGAGGAACCTCAACCCCCCCGACGGTGCGGCAGAc                                                                                      <  2:63126/247‑1 (MQ=255)

                                                                                                                                                                tgtgtgCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGCTCAGCATGGTATGGCTGAATATCGTCAGCCACGGCCGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACAATGACGAGGAACATCACCACAACCGACTGTGCGGCAGACGac                                                                                   >  2:181179/1‑250 (MQ=255)

                                                                                                                                                                 gtgtgCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGaa                                                                                   <  1:264207/250‑1 (MQ=255)

                                                                                                                                                                   gtgCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGTGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGc                                                                                 <  1:82156/250‑1 (MQ=255)

                                                                                                                                                                     gCCGGGGATGAGCGTCCACGGGGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCATCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAg                                                                              <  2:230886/251‑1 (MQ=255)

                                                                                                                                                                     gCCGGGGATGAGCGTCACCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAg                                                                              <  1:392164/251‑1 (MQ=255)

                                                                                                                                                                       cGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCCTTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGcc                                                                                >  2:130434/1‑247 (MQ=255)

                                                                                                                                                                           gATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATcc                                                                          >  1:537559/1‑249 (MQ=255)

                                                                                                                                                                              gAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTcc                                                                     <  1:118211/251‑1 (MQ=255)

                                                                                                                                                                               aGCGTCATCGGCGCGGCATAGACTGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGAGCGGCCGACGAAGCCAGATCCAGTCCg                                                                    >  2:167525/1‑251 (MQ=255)

                                                                                                                                                                                 gggcATCGGCGCGGCAAAGACGGCCTGTTTATCCAGCGGCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGt                                                                  <  2:437512/248‑1 (MQ=255)

                                                                                                                                                                                 cGTCATCGGCGCGGCATAGACGGCCTGTTTACCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCAGTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGt                                                                  <  1:524340/251‑1 (MQ=255)

                                                                                                                                                                                  gTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGccc                                                                                                                                          >  2:328565/1‑176 (MQ=255)

                                                                                                                                                                                  gTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGcac                                                                                                                                          <  1:328565/178‑1 (MQ=255)

                                                                                                                                                                                                aTAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTa                                                   <  1:553356/251‑1 (MQ=255)

                                                                                                                                                                                                  aGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAg                                                  <  1:168531/250‑1 (MQ=255)

                                                                                                                                                                                                  aGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGa                                                 <  1:458859/251‑1 (MQ=255)

                                                                                                                                                                                                  aGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGa                                                 <  1:325473/251‑1 (MQ=255)

                                                                                                                                                                                                            tttATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCACGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTa                                                   >  1:372094/1‑239 (MQ=255)

                                                                                                                                                                                                              tATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATc                                      <  1:458940/250‑1 (MQ=255)

                                                                                                                                                                                                                tCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGc                                   >  1:543772/1‑251 (MQ=255)

                                                                                                                                                                                                                     cGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGcgtcgt                              >  1:11954/1‑251 (MQ=255)

                                                                                                                                                                                                                      gTCGCCGCGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAAc                                                                                                                                                                         <  1:358455/111‑1 (MQ=255)

                                                                                                                                                                                                                      gTCGCCGAGACA‑AACGCAACGTACAGCGGGAAAAGGATCACCGCGCTCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAAc                                                                                                                                                                         >  2:358455/1‑111 (MQ=255)

                                                                                                                                                                                                                         gccgcGACA‑AACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGGCgtggtg                          >  1:172851/1‑251 (MQ=255)

                                                                                                                                                                                                                                 caaaacccACGTACAGCGTGAAATGGATCACCGATATCCGGATGATCATCATGGTATGGCTGAATATCGTCAGCAACAGACTGTTCCCAAGCATTGGTAACGCACCTTGCTTTCAAAATAGCGGAACTGCTCCACCGTCAGCACGATGCCGAGGAACATCAACACCACCGAGTTTGCTGCAGACGAAGCCAGATCTAGTCCGGTAAAACCTTCGCGGTAGATCTTATAACTCAGcgtcttgttggtttgaa                   <  2:316190/245‑14 (MQ=255)

                                                                                                                                                                                                                                 a‑aaCGCCACGTACAGCGGGAAAAGGATCACCGCAATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAAc                  >  1:331897/1‑251 (MQ=255)

                                                                                                                                                                                                                                   aacgcaacgaccaccGGGAAAAGGATCACCGCGATCCCGAGGCTCAGCATGGTATGGCTGAATATAGTCAGCCACGGACGGATCTCAATCCTTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGACCATCAACACCACCGACGGAGCGGCAGCCGAAGCCAGATCCAGTCCGGTAAACCCTCCCCGGGAGATCTTATCAATCACCGCCGTGGTGGCCTGAAcc                 >  2:76799/15‑251 (MQ=255)

                                                                                                                                                                                                                                    aCGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCg                >  1:200709/1‑251 (MQ=255)

                                                                                                                                                                                                                                            tACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGCAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCccgccg        >  1:102559/1‑251 (MQ=255)

                                                                                                                                                                                                                                               aGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGCACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTAGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGAc     >  1:232725/1‑251 (MQ=255)

                                                                                                                                                                                                                                                  gggAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTg  >  1:240092/1‑251 (MQ=255)

CGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCA‑GAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACA‑AACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTG  >  NC_000913/3589711‑3590201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`