Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,588,787	G→A	P132S (CCG→TCG)	ugpC ←	sn‑glycerol 3‑phosphate ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,588,787	0	G	A	98.8%	233.6 / NA	84	P132S (CCG→TCG)	ugpC	sn‑glycerol 3‑phosphate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base A (32/50);  minor base C (0/1);  total (32/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

AACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAAC‑GTAGAGTGAAGTCGTTT‑TCAGGCGAC‑GGTGCAACTGTTGCAGTTCAAGACGCATCTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTCCAGCCCGGC  >  NC_000913/3588545‑3589030                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     aaCACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAAC‑GTAGAGTGAAGTCGTTT‑TCAGGCGAC‑GGTGCAACTGTTGCAGTTCAAGACGCATCTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGCCCGCCGGAAAGCTCGCGCGAGCGACGtt                                                                                                                                                                                                                                              >  1:547697/1‑251 (MQ=255) aaCACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAAC‑GTAGAGTGAAGTCGTTT‑TCAGGCGAC‑GGTGCAACTGTTGCAGTTCAAGACGCATCTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGtt                                                                                                                                                                                                                                              <  1:425113/251‑1 (MQ=255)  acacCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAAC‑GTAGAGTGAAGTCGTTT‑TCAGGCGAC‑GGTGCAACTGTTGCAGTTCAAGACGCATCTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGttt                                                                                                                                                                                                                                             <  1:210001/251‑1 (MQ=255)       gccgtacagccccagtACTCGCGGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAAC‑GTAGGGTGAAGTCGTTT‑TCAGGCGAC‑GGTGCAACTGTGGCAGTTCAAGACGCACCTGCACG‑CGCAGCTTGGCAGCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AAAACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCa                                                                                                                                                                                                                                        <  1:100855/236‑1 (MQ=255)       gccgTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAAC‑GTAGAGTGAAGTCGTTT‑TCAGGCGAC‑GGTGCAACTGTTGCAGTTCAAGACGCATCTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGCCCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGc                                                                                                                                                                                                                                         >  2:215259/1‑250 (MQ=255)       gccgTTCATCACCATTACCCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAAC‑GTAGAGTGAAGTCGTTT‑TCAGGCGAC‑GGTGCAACTGTTGCAGTTCAAGACGCATCTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGTCGTTTGAGCa                                                                                                                                                                                                                                        <  1:379873/251‑1 (MQ=255)          gTTCATCACCATTACGCGCTGGGCGGGCGCCCTCGCGTAACCCTGATCGTGAGTTAC‑GTAGAGTGAAGT‑GTTTGTCGGGCGAC‑GGGGCAACTTTTGCAGGTCAAGCCGCATCTGCACG‑CGCAGCTTGGCATCGAGGAGAGAGAGCGTCTCATCAAACAGG‑AACACCGCCGTATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGATCAGAc                                                                                                                                                                                                                                     <  2:343605/251‑1 (MQ=255)            tcatcaCCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAAC‑GTAGAGTGAAGTCGTTT‑TCAGGCGAC‑GGTGCAACTGTTGCAGTTCAAGACGCATCTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGAcc                                                                                                                                                                                                                                    <  1:425270/250‑1 (MQ=255)            tcatcaCCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAAC‑GTAGAGTGAAGTCGTTT‑TCAGGCGAC‑GGTGCAACTGTTGCAGTTCAAGACGCATCTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑AGCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGAcc                                                                                                                                                                                                                                    <  1:523169/250‑1 (MQ=255)              atcaCCTTTACTCGCTGGGCGAGCGTCATCCCTTCAACTGGATCGTGAGTAAC‑GATTAGTTAAGTCGTTT‑TCAGGCGAC‑GGTGCAACTGTTGCAGTTCAAGACGCTTCTGCCCG‑CGCAGCTTTGCATCGAGGTTAGAGAGCGGCTCAACAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCTAGCGACGTGTGAGCAGACCGTc                                                                                                                                                                                                                                 <  2:379631/251‑1 (MQ=255)              atcaCCATTACTCGCTGGGCGAGCGTCCTCGCTTCAACCTGATCGTGAGTAAC‑GTAGAGTGAAGTCGTTT‑TCAGGCGCC‑GGTGCAACTGTTGCAGTTCAAGACGCATCTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCTCCGGATCGCGCACA‑ATCGCCCGGCCCATCCCCCCACGCTGGCCCTGACCGCCGCAAAGCTCGCGCGAGCggcgatgtacaagcccgt                                                                                                                                                                                                                                  >  2:118110/1‑233 (MQ=255)                     ttACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAAC‑GTAGAGTGAAGTCGTTT‑TCAGGCGAC‑GGTGCAACTGTTGCAGTTCAAGACGCATCTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTcc                                                                                                                                                                                                                          >  1:113894/1‑251 (MQ=255)                          cGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAAC‑GTAGAGTGAAGTCGTTT‑TCAGGCGAC‑GGTGCAACTGTTGCAGTTCAAGACGCATCTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAAt                                                                                                                                                                                                                     >  1:482193/1‑251 (MQ=255)                                 ggAGCGTCATCGCTTCAACCTGATCGTGAGTAAC‑GTAGAGTGAAGTCGTTT‑TCAGGCGAC‑GGTGCAACTGTTGCAGTTCAAGACGCATCCGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGcgcc                                                                                                                                                                                                              <  1:107823/250‑1 (MQ=255)                                     cGTCATCGCTTCAACCGGATCGTGAGTAAC‑GTAGAGTGAAGTCGTTT‑TCAGGCGAC‑GGTGCAACGGTTGCAGTTCAAGCCGCATCTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGctt                                                                                                                                                                                                          <  2:134175/251‑1 (MQ=255)                                          tcgcttcagctggATCGTGTGTAAC‑GTATAGTGAAGTCTGTT‑TCTGGCGAC‑GTTTCAACTTTTGCAGTGAAAGACGCATTTGCAGG‑CGCAGCTGGGTATCGAGGTTAGTGAGCGGCTCATCAAACAGG‑AACACCGCCGGACCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCGTGCGCTGTCCGCCGGAAAGCTCGCGCGAGCGTCGTGTGAGCATCCCGTCCAGCTCCAGAATGCGCGCCGcttctt                                                                                                                                                                                                       <  2:331465/237‑1 (MQ=255)                                             cTTCAACCTGATCGTGAGTAAC‑GTAGAGTGAAGTCGTTT‑TCAGGCGAC‑GGTGCAACTGTTGCAGTTCAAGACGCATCTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAAcg                                                                                                                                                                                                  >  1:318251/1‑251 (MQ=255)                                              ttCAACCTGATCGTGAGTAAC‑GTAGAGAGAAGTCGTTT‑TCAGGCGAC‑GGTGCAACTGTTGCAGTTCAAGACGCATCTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTCACCGCCGGAAAGCTCGCGCGAGCGACGCTTGAGCAGACCGTCCAGCTCCAGCATGCGCGCCGCCTCTTTAAcgc                                                                                                                                                                                                 >  2:11275/1‑251 (MQ=255)                                                    cggATCGTGAG‑AACAGTAGAGTGGATGCGTTT‑TCAGTACAC‑GGTTCAACCGTTAGAGTTCAAGACGCATTTGCAGG‑CGGAGGTTGGAATCGAGGTAAGAGAGCGTCTCATCAAACAGG‑AACCACGCCGGATCGCGCACA‑ATCGCGCGGCCCAACGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCCCTCGCGACGGATGAGCAGACCGTCCATCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCgg                                                                                                                                                                                           <  2:59583/249‑1 (MQ=255)                                                     tGATCGTGAGTAAC‑GTAGAGTGAAGTCGTTT‑TCAGGCGAC‑GGTGCAACTGTTGCAGTTCAAGACGCATCTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGc                                                                                                                                                                                          >  1:261474/1‑251 (MQ=255)                                                      gATCGTGAGTAAC‑GTAGAGTGAAGTCGTTT‑TCAGGCGAC‑GGTGCAACTGTTGCAGTTCAAGACGCATCTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGc                                                                                                                                                                                          <  1:111936/250‑1 (MQ=255)                                                          gTGAGTAAC‑GTAGAGTGCAGTCGTTT‑TCAGGCGAC‑GGTGCAACTGTTGCAGTTCAAGACGCATCTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCCGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCACCCCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCCAttt                                                                                                                                                                                     >  2:475720/1‑251 (MQ=255)                                                                aaC‑GGAGAGTGATGTCGTCT‑TCAGG‑GACCGGCGCAACGGTGGCAGTTACAGCCGTGTCTGCACG‑CGCAGCTGGGCTTCGAGGTTCGCGCGCGGCTCATCAAACCGT‑AACACCGACGGAGCGCGCTCA‑ATCGCGCGGACCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGCCGTTTGAGCAGACCTTCCAGCTCTAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTgctgct                                                                                                                                                                               <  2:433049/251‑1 (MQ=255)                                                                  atcgtgaactgacgtcgtgtccAGGCGTC‑GGTGTAACGGTGGCAGTTCAAGACTCATCTGCGCG‑CGCAGCTTGGATTCGAGGTGAGATAGCGGCTCATCAAACAGG‑AACTCCGCCGTATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGGTTGAGCAGACCGCCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGTCAATTTgatgct                                                                                                                                                                               <  2:39770/230‑1 (MQ=255)                                                                     tAGAGTGAAGTCGTTT‑TCAGGCGAC‑GGTGCAACTGTTGCAGTTCAAGACGCATCTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCa                                                                                                                                                                                                                         <  1:380687/205‑1 (MQ=255)                                                                            aaGTCGTTT‑TCAGGCGAC‑GGTGCAACTGTTGCAGTTCAAGACGCATCTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCcg                                                                                                                                                                   >  1:86990/1‑251 (MQ=255)                                                                             aGTCGTTT‑TCAGGCGAC‑GGTGCAACTGTTGCAGTTCAAGACGCATCTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCACACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGGGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCcgc                                                                                                                                                                  >  1:549848/1‑251 (MQ=255)                                                                                atgtt‑tCAGGCGAC‑GGTGTAACTGTTGCTGTTGAAGCCGCATCTG‑ACGGCGCAGCTTGGCATCGGTGTTTGAGAGCGGCTAATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCCGGCGCTGACCGCCGGAAAGTTCGCGCGAGCGACGTTTGAGCAGATCGTCCATCTCCAGAATGCGCGCCGATTCTTTAACGCGCTCGGGAATTTGCTGCTTGCCC‑ATGCCGCGa                                                                                                                                                                <  1:561064/247‑1 (MQ=255)                                                                                 gTTT‑TCAGGCGAC‑GGTGCAACTGTTGCAGTTCAAGACGCATCTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGact                                                                                                                                                              >  1:262870/1‑249 (MQ=255)                                                                                 gTTT‑TCAGGCGAC‑GGTGCAACTGTTGCAGTTCAAGACGCATCTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAAt                                                                                                                                                              <  2:113239/251‑1 (MQ=255)                                                                                      tCAGGCGAC‑GGTGCACCTGTTGCAGTCCAAGACGCACTTGCACG‑CTCAGCTTGGCATCGAGGTTAGAGAGCTGCTCATAAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCTACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAAtttt                                                                                                                                                           <  2:91878/250‑1 (MQ=255)                                                                                       cAGGCGAC‑GGTGCAACTGTTGCAGTTCAAGACGCATCTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAAtttca                                                                                                                                                          >  1:560275/1‑248 (MQ=255)                                                                                          ggctacggtcaaaCTTTTGCATTCCAAGACGCATCTTCCCG‑CGCCGCTTGGCATCGAGTTTAGATAGCAGCTAATCAACCAGC‑AATCCCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCCCACGCTGGCGCTGACCGCCGGAAAGCCCGCGCGAGCGACGTTTGAGCAGCCCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATCCCGCGAATCTTCAGc                                                                                                                                                       <  2:141458/240‑1 (MQ=255)                                                                                          gCGAC‑GGTGCAACTGTTGCAGTTCAAGACGCATCTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGcc                                                                                                                                                      >  1:156647/1‑251 (MQ=255)                                                                                                   gCAACTGTTGCAGTTCAAGACGCATCTGCACG‑TGCATCTTGGCATCGAGGTTAGAGAGCGGCTCATCAACCAGG‑AACACCGCCGGAGCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGATAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGcc                                                                                                                                              <  1:540211/251‑1 (MQ=255)                                                                                                   gCAACTGTTGCAGTTCAAGACGCATCTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGcc                                                                                                                                              <  1:253320/251‑1 (MQ=255)                                                                                                   ccaccTGTTGCTGTACAAGACGCATCTGCACG‑CGCATCTTGGCATCGAGGTTAGCGGGCGGCCCATCAAACAGG‑ACCACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGcc                                                                                                                                              <  2:238926/247‑1 (MQ=255)                                                                                                       cggtggCAGTTCAAGACGCATCTGCACG‑CCCAGCTTGGCATCGAGGTTAGAGATCGCCTCATCAAACAGG‑AACCCCGCCGGACCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGCCATGt                                                                                                                                          <  2:134368/246‑1 (MQ=255)                                                                                                       cTGTTGCAGTTCAAGACGCATCTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGCCATGt                                                                                                                                          >  1:495185/1‑251 (MQ=255)                                                                                                         ggtgcagtgaaaGACGCATCGGCACG‑CACAGCTTGGCATCGAGGTTAGAGAGCGGCTAACCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCAGCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCGTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGCCATGttt                                                                                                                                        <  2:389295/241‑1 (MQ=255)                                                                                                           ttcagtccaccacgcagctcaccg‑cgCACCTTGGCATCGGTGTTAGAGAGCGGCTCAGAAACCAGG‑ACCACCGCCGGATCGCGCACA‑TTCGCGCGGCCCATCTCCACACGCTTGCGCTGACCGCCGGAAAGCGCCCTCTAGCGACGTTTGAGCAGACCGTCCAGCTCCAGATTGCTCGCCGCTTCTTTAACGCGCTCGGCAATTTGCGGCTTGCCC‑AGGTCGCGAAGTTTAATCCCCCGCGCCATGtttt                                                                                                                                       <  2:75580/228‑1 (MQ=255)                                                                                                           tGCAGTTCAAGACGCATCTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGCCATGtttt                                                                                                                                       <  1:215259/250‑1 (MQ=255)                                                                                                            gCAGTCCAAGCCGCACCTGCACG‑CCCAGCTTGGCATCGCGGTTAGAGAGCGTCTCACCAAACAGG‑ACCACCGCCGGATCGCGCACA‑ACCGCGCGGCCCTGCGCCACACGCTGGCGCTGACCGCCGGAAAGCGCGCGCGAGCGTCGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGCCATGtttgca                                                                                                                                     <  2:284673/251‑4 (MQ=255)                                                                                                                  cAAGACGCATCTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCCTCAGACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCTGCCCATCGCCCCACGCTGGCGCTGCCCGCCGGAAGGCTCGCGCGAGCGACGTTTGAGCAGACAGTCCAGCTCCCGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCCGTGC‑GCGACTTTTCAGCCCCCACGCCATGTTTTCTTCGacc                                                                                                                               >  2:162006/1‑250 (MQ=255)                                                                                                                     gACGCATCTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGacaatc                                                                                                                            >  1:423604/1‑248 (MQ=255)                                                                                                                      aCGCATCTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTc                                                                                                                            >  1:351435/1‑250 (MQ=255)                                                                                                                          aTCTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGACATGTTTTCTTCGCCACTCatatc                                                                                                                       >  2:216634/1‑250 (MQ=255)                                                                                                                            cTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGcttc                                                                                                                                                                                                         <  2:247449/168‑1 (MQ=255)                                                                                                                            cTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGcttc                                                                                                                                                                                                         >  1:247449/1‑168 (MQ=255)                                                                                                                            cTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCa                                                                                                                                                  >  1:103630/1‑222 (MQ=255)                                                                                                                              gCACG‑CGCAGCTTGGCATCGAGGTTAGAGGGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAAGTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGa                                                                                                                   <  1:505692/251‑1 (MQ=255)                                                                                                                               cACG‑CGCGGCTGGGCATCGAGGTAAGAGAGCGCCTCATCAAACAGG‑ACCACCGCCGGATCGCGCACA‑ATCGCGCGGCCCAGCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGAt                                                                                                                  <  2:86990/251‑1 (MQ=255)                                                                                                                                aCG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAACTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCACCCACGCCATGTTTTCTTCGACCCTCATATGCGGAt                                                                                                                  >  2:210136/1‑250 (MQ=255)                                                                                                                                            ggCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGt                                                                                                      >  1:124573/1‑251 (MQ=255)                                                                                                                                                 cGAGGTTAGAGAGCGGCTCAGCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTg                                                                                                  <  1:337925/250‑1 (MQ=255)                                                                                                                                                        agagagCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACAcc                                                                                           >  1:221387/1‑250 (MQ=255)                                                                                                                                                           ggctgggccactaaaCAGGGAACACTGCCGGATCGGGCACA‑ACCGTGCGGCCCATCGTCACTCGCTGGCGCGGACTGCCGGAAAGCTCGCGCGAGCGACGTTGGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCTAATTTGCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCTTAGTTCTGGAACACCATc                                                                                        <  2:549848/239‑1 (MQ=255)                                                                                                                                                           gacgggtgcaataaaCAGG‑AACTCCTCCGGCTCGCGC‑CACATCGTGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCGCGCACGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGTAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGCCATGTTTGCTGCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCagcg                                                                                       <  2:257066/239‑4 (MQ=255)                                                                                                                                                              cGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGAGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATAAGCGGATACAGCGCGTAGTTCTGAAACACCATCGCa                                                                                     >  2:203949/1‑250 (MQ=255)                                                                                                                                                                    agcaAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGGTCGCGGGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCcgc                                                                              <  1:236328/249‑1 (MQ=255)                                                                                                                                                                     ccaAACAGG‑ACCACCGCCGGACCGCGAACC‑ACCGCCCGGCCCCTCGCCCCCCCCTGGCGCCTACCGCCGTAAATCTCGCGCGATCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCcgcg                                                                             <  2:187281/250‑1 (MQ=255)                                                                                                                                                                      caAACAGG‑AAAACAGCCGGATCGCGCACA‑ATAGCGCGGCCCATCGCCACACACTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGGCGTTTTAGCAGACCGTCCCGCTCCAAAATGCGCGCCTCTTCTTTAACGCGATCGGCAGTTTGCTTCTTGCCC‑ATGCCGCGACTTTTCAGCCCCCACGCCATGATTTCTTCGACAATCATATACGGTTAAAGCGCGTAGGTCTGGAACACCATCGCCATCCcgca                                                                             >  2:347000/1‑249 (MQ=255)                                                                                                                                                                         ccAGG‑ACCACCGCCGGATCGCGCACA‑ACCGCGCGGCCCATCGCCACCCGCTGGCGCTGCCCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGagct                                                                         <  2:202885/250‑4 (MQ=255)                                                                                                                                                                          ccaggacccccccctgaccgctcaca‑ATCGCGCGGCCCTTCGCCACACGCTGGCGCGGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGGCCAGCTCCAGAATTCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATAGGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCt                                                                         <  2:547697/229‑1 (MQ=255)                                                                                                                                                                           aGG‑AACACCGCCGGATGCCTCAAA‑ACCGCGCGGCCCCTCGCCAGACGCTGCCTCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTTAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCAACGCAATCCCGCGATCttt                                                                       <  2:388800/251‑1 (MQ=255)                                                                                                                                                                                accccgccgGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGc                                                                   <  1:36286/248‑1 (MQ=255)                                                                                                                                                                                     gccgGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCAt                                                              <  1:203949/251‑1 (MQ=255)                                                                                                                                                                                       cgGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGGATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATACGGCTAAAGCGAGTATTTCTGGAACACCATCGCAATCCCGCGACCTTTTTGCTCCAttt                                                            >  2:523216/1‑251 (MQ=255)                                                                                                                                                                                          aTCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTc                                                           >  1:89411/1‑249 (MQ=255)                                                                                                                                                                                               cccca‑ATCGCGCGCCCAACCGCCACTCGCGGGCGCCGCTCGCCGGAAAGCCTGCGCGAGCGACGGTTGAGCATACCGGCCAGCCCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGTCTCCATTTCAGTCAcg                                                    <  2:124573/248‑1 (MQ=255)                                                                                                                                                                                                      ccgctcgGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCTCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCTGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTgg                                              <  1:524007/246‑1 (MQ=255)                                                                                                                                                                                                         cgcgGCCCATCGCCACACGCTTGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCTTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCg                                           <  1:239701/251‑1 (MQ=255)                                                                                                                                                                                                           cgGCCCATCGCCACCCGCTGGCGCTATCTGCCGGAAAGCTGGCGCGGGTGACGTTTGAGCAGGCAGTCCAGCTCCAGAATGAGCGCCGCTTCTTTAACGCGCTCGGCATTTTGCTGCTTGCGC‑AGGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCATCGACACTCATATGCGTATAAAGCGCGTAGTTCTGGAACACCATCTCACTCCCTCGATCTTTTGGCTCCATTTCAGTCCCGCGCTGGTCGtg                                         <  2:318251/251‑2 (MQ=255)                                                                                                                                                                                                            ggCCCATCGCCACACGCTTGCGCTGACCGCCGGAAAGCTCGCGCGAGCGTCGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCTAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTg                                        <  1:325046/251‑1 (MQ=255)                                                                                                                                                                                                                   cgcccaccGCTGGCGCTGACCTCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCGTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGa                                 <  2:156647/244‑1 (MQ=255)                                                                                                                                                                                                                   cGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGa                                 <  1:475720/251‑1 (MQ=255)                                                                                                                                                                                                                     ccACGCGCGGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGATCATACCGTCCAGCTCCAGAATGCGCTCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGata                               <  2:122400/251‑1 (MQ=255)                                                                                                                                                                                                                      cacaCGCTGGCGCTGACCGCCGGCAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGTTCTTTTGGCTCCATTTCAGTCACACGCTGGTCGTTGATCCAGatat                              >  2:223452/1‑251 (MQ=255)                                                                                                                                                                                                                         acGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGc                           >  1:160754/1‑251 (MQ=255)                                                                                                                                                                                                                              ggccctctccgacgGAAATCTCGCGCGAGCGGCTTTTGGGCAGACCTTCCTGCTCCAGAATGACAGCAGCTGCTTTAACGCGCTCGTCAATTGGCTCCCTGCCC‑ATGCCGCGAATTGTCAGCCCCCACGCCATGTTTTCTTCGACACTCACATGCTGATTAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCACTTCAGTCTCGCGCTGGGCGTTGATCCCTATATCGCCta                        <  2:157056/237‑2 (MQ=255)                                                                                                                                                                                                                                    gACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCAcc                <  1:137810/251‑1 (MQ=255)                                                                                                                                                                                                                                           ggAAAGCTCGCGCGAGAGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTCCa         <  1:126137/251‑1 (MQ=255)                                                                                                                                                                                                                                            gAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGAGAATTTTCAGCTCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCACGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTcc          >  1:292491/1‑249 (MQ=255)                                                                                                                                                                                                                                                   cgcgcgAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTCCAGCCCGGc  >  1:277721/1‑250 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     AACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAAC‑GTAGAGTGAAGTCGTTT‑TCAGGCGAC‑GGTGCAACTGTTGCAGTTCAAGACGCATCTGCACG‑CGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGG‑AACACCGCCGGATCGCGCACA‑ATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCC‑ATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTCCAGCCCGGC  >  NC_000913/3588545‑3589030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑