Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,581,850	G→A	intergenic (+224/‑13)	yhhY → / → yhhZ	N‑acetyltransferase YhhY/putative endonuclease YhhZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,581,850	0	G	A	100.0%	76.3 / NA	24	intergenic (+224/‑13)	yhhY/yhhZ	N‑acetyltransferase YhhY/putative endonuclease YhhZ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/16);  total (8/16)

ATATGGCGCGGGTGAAGTAAGATAGTGCCCTTTTTCTGAGATGGAAAAAGGGTGTCATTCAAAATCGACATACCTTCCTTTAAGGTATTTATTTGCCCAATACATATATTGAGATTTAACTCATAATTACTTCCTAAAGTGTAATATTTTATTTTTTAATATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAGGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGAAGCATCTCCGCAGGTTGTGGGACTTCTGAGTCTACAGGTAATCGTTGGCAGAGCGGGCATGAGGATGAAATATTTACATTCTCACTCTTAAATAATATTAATAATACGGGGCTTGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATT  >  NC_000913/3581607‑3582077                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                    atatGGCGCGGGTGAAGTAAGATAGTGCCCTTTTTCTGAGATGGAAAAAGGGTGTCATTCAAAATCGACATACCTTCCTTTAAGGTATTTATTTGCCCAATACATATATTGAGATTTAACTCATAATTACTTCCTAAAGTGTAATATTTTATTTTTTAATATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACtttt                                                                                                                                                                                                                              <  1:273358/251‑1 (MQ=255)                aGTAAGATAGTGCCCTTTTTCTGAGATGGAAAAAGGGTGTCATTCAAAATCGACATACCTTCCTTTAAGGTATTTATTTGCCCAATACATATATTGAGATTTAACTCATAATTACTTCCTAAAGTGTAATATTTTATTTTTTAATATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGTAata                                                                                                                                                                                                               <  1:291874/251‑1 (MQ=255)                  tAAGATAGTGCCCTTTTTCTATGATGGAAAAAGTGTGTCAATCAAAATCGACATACCTTCCTTTAAGGTATTTATTTGCCCAATACATATATTGAGATTTAACTCATAATTACTTCCTAAATTGTTATATTTTATTTTTTAATATATACGCCTACAATTTCTTGGAGTAAATAAAAAACAAGTAACAATCATAAGATTGCCAGTGATAAAATAGCATGCCATAAAAAGACGTTTCAGGTATGAGTAatat                                                                                                                                                                                                              <  2:566859/250‑1 (MQ=255)                                     tGAGATGGAAAAAGGGTGTCATTCAAAATCGACATACCTTCCTTTAAGGTATTTATTTGCCCAATACATATATTGAGATTTAACTCATAATTACTTCCTAAAGTGTAATATTTTATTTTTTAATATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACgg                                                                                                                                                                                          >  1:478726/1‑251 (MQ=255)                                       agaTGGAAAAAGGGTGTCATTCAAAATCGACATACCTTCCTTTAAGGTATTTATTTGCCCAATACATATATTGAGATTTAACTCATAATTACTTCCTAAAGTGTAATATTTTATTTTTTAATATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACggg                                                                                                                                                                                         >  1:327313/1‑250 (MQ=255)                                                 aGGGTGTCATTCAAAAGAGACATACCTTTCTTTAATGTATTTATTTGCCCAATACATATAATGAGATTTAACTCATAATTACTTCCTAAAGTGTAATATTTTATTTTTTAAAATATACGCCTACATTTTCCAGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTATTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACTACAAg                                                                                                                                                                              <  2:395936/251‑1 (MQ=255)                                                                  cGACATACCTTCCTTTAAGGTATTTATTTGCCCAATACATATATTGAGATTTAACTCATAATTACTTCCTAAAGTGTAATATTTTATTTTTTAATATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAATGCTTTTCAGGGATGAGGAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGAAGCATCTCCGCAgg                                                                                                                                                              <  1:360961/250‑1 (MQ=255)                                                                    aCATACCTTCCTTGAAAGTATTTAGTAGCCCAAAACATATAATGATATTTAACTCATAATTACTTCCGAAAGTGTAATATTTTATTTTTGAATATATACGCCTACAAGTTCCTTGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTGTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGGAGCATCTCCTCAGGTtg                                                                                                                                                           <  2:568524/251‑1 (MQ=255)                                                                                   aGGTATTTATTTGCCCAATACATATATTGAGATTTAACTCATAATTACTTCCTAAAGTGTAATATTTTATTTTTTAATATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGAAGCATCTTCGCAGGTTGTGGGACTTCTGAGTc                                                                                                                                            >  1:32601/1‑251 (MQ=255)                                                                                           atttGCCCAATACATATATTGAGATTTAACTCATAATTACTTCCTAAAGTGTAATATTTTATTTTTTAATATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGAAGCATCTCCGCAGGTTGTGGGACTTCTGAGTCTACAGGt                                                                                                                                     <  1:254614/250‑1 (MQ=255)                                                                                                             ttGAGATTTAACTCATAATTACTTCCTAAAGTGTAATATTTTATTTTTTAATATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGAAGCATCTCCGCAGGTTGTGGGACTTCTGAGTCTACAGGTAATCGTTGGCAGAGCGGGc                                                                                                                  >  1:258779/1‑251 (MQ=255)                                                                                                                       aCTCATAATTACTTCCTAAAGTGTAATATTTTATTTTTTAATATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGAAGCATCTCCGCAGGTTGTGGGACTTCTGAGTCTACAGGTAATCGTTGGCAGAGCGGGCATGAGGATGa                                                                                                        <  2:166271/251‑1 (MQ=255)                                                                                                                        ttCATAATTAATTCATAAAGTGTAATATTTTATTTTTTAATATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGAAGCATCTCCGCAGGTTGTGGGACTTCTGAGTCTACAGGTAATCGTTGGCAGAGCGGGCATGAGGATGa                                                                                                        >  2:256452/2‑250 (MQ=255)                                                                                                                                             gtAATATTTTATTTTTTAATATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGAAGCATCTCCGCAGGTTGTGGGACTTCTGAGTCTACAGGTAATCGTTGGCAGAGCGGGCATGAGGATGAAATATTTACATTCTCACTCTTa                                                                                  <  1:35953/251‑1 (MQ=255)                                                                                                                                              tAATATTTTATTTTTTAATATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGAAGCATCTCCGCAGGTTGTGGGACTTCTGAGTCTACAGGTAATCGTTGGCAGAGCGGGCATGAGGATGAAATATTTACATTCTCACTCTTa                                                                                  >  1:347781/1‑250 (MQ=255)                                                                                                                                                     ttattttTTAATATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGAAGCATCTCCGCAGGTTGTGGGACTTCTGAGTCTACATGTAATCGTTGGCAGAGCGGGCATGAGGATGAAATATTTACATTCTCACTCTTAAataata                                                                           <  1:401464/250‑1 (MQ=255)                                                                                                                                                              aaTATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGAAGCATCTCCGCAGGTTGTGGGACTTCTGAGTCTACAGGTAATCGTTGGCAGAGCGGGCATGAGGATGAAATATTTACATTCTCACTCTTAAATAATATTAATAATAc                                                                 >  1:319299/1‑251 (MQ=255)                                                                                                                                                                   ataCGCCTACAATTTCCTGGAGTAAATAAATAACAATTAAAAAGCATAATATTGCCATGGATAAAATAGCATGCCATAAAAATACGTTTCAGGGATTAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGAAGCATCTCCGCAGGTTGTGGGACTTCTGAGTCTACAGGTAATCTTTGGCAGAGCGGGCATGAGGATGAAATATTTACATTCTCTCTCTTAAATAATATTAATAATATGGGGc                                                            <  2:449554/251‑1 (MQ=255)                                                                                                                                                                                          aaataaatAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGAAGCATCTCCGCAGGTTGTGGGACTTCTGAGTCTACAGGTAATCGTTGGCAGAGCGGGCATGAGGATGAAATATTTACATTCTCACTCTTAAATAATATTAATAATACGGGGCTTGGTTCACAGTTCCATGGtata                                     <  1:256452/251‑1 (MQ=255)                                                                                                                                                                                             taaataaCAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGAAGCATCTCCGCAGGTTGTGGGACTTCTGAGTCTACAGGTAATCGTTGGCAGAGCGGGCATGAGGATGAAat                                                                                                     <  1:354416/184‑1 (MQ=255)                                                                                                                                                                                             taaataaCAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGAAGCATCTCCGCAGGTTGTGGGACTTCTGAGTCTAAAGGAAATCGTTGGCAGAGCGGGCATGAGGATGAAat                                                                                                     >  2:354416/1‑184 (MQ=255)                                                                                                                                                                                                         aaCAAGCATAATATTGCAATTGATAAAATAGAATGTCATAAAAAGACTTTTCAGGTATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACTAGGAAGCATCTCCGCAGGTTGTGGGACTTCTGAGTCTACAGGTAATCGTTGGCAGAGCGGGCATGAGGATGAAATATTTACATTCTCACTCTTAAATAATATTAATAATACGGGGCTTGGTTCACAGTTCCATGGTATAACATTTTGTAaatt                       <  2:444858/250‑1 (MQ=255)                                                                                                                                                                                                                         ccATTGATAAAATAGCAGGCCATAAAAAGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGAAGCATCTCCGCAGGTTGTGGGACTTCTGAGTCTACAGGTAATCGTTGGCAGAGCGGGCATGAGGATGAAATATTTACATTCTCACTCTTAAATAATATTAATAATACGGGGCTTGGTTCACAGTTCCATGGTATAACATGTTGTAAATTAATTGATAAAAGCACTc      <  2:221384/251‑1 (MQ=255)                                                                                                                                                                                                                              gATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGAAGCATCTCCGCAGGTTGTGGGACTTCTGAGTCTACAGGTAATCGTTGGCAGAGCGGGCATGAGGATGAAATATTTACATTCTCACTCTTAAATAATATTAATAATACGGGGCTTGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCatt  <  1:308973/250‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                    ATATGGCGCGGGTGAAGTAAGATAGTGCCCTTTTTCTGAGATGGAAAAAGGGTGTCATTCAAAATCGACATACCTTCCTTTAAGGTATTTATTTGCCCAATACATATATTGAGATTTAACTCATAATTACTTCCTAAAGTGTAATATTTTATTTTTTAATATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAGGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGAAGCATCTCCGCAGGTTGTGGGACTTCTGAGTCTACAGGTAATCGTTGGCAGAGCGGGCATGAGGATGAAATATTTACATTCTCACTCTTAAATAATATTAATAATACGGGGCTTGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATT  >  NC_000913/3581607‑3582077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑