Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,506,393	T→C	D315G (GAC→GGC)	yhfT ←	uncharacterized protein YhfT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,506,393	0	T	C	100.0%	158.4 / NA	52	D315G (GAC→GGC)	yhfT	uncharacterized protein YhfT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/19);  total (33/19)

GAAGTAAATGGCAACCGCGATAGAGAATCCGGTATAACCCGCCATCTTAATTGCTGCGAAAATCGAACCGACCAGCAGCGCCACTTCCATCAGCATATTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGTCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTCCCACG  >  NC_000913/3506149‑3506635                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   gAAGTAAATGGCAACCGCGATAGAGAATCCGGTATAACCCGCCATCTTAATTGCTGCGAAAATCGAACCGACCAGCAGCGCCACTTCCCTCAGCATATTCATGGCGTTACGGATGTTATCCGACGCATTACGCCCCGACGGGTAGCGTCCCAGCCCTTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCg                                                                                                                                                                                                                                              >  1:34578/1‑251 (MQ=255)           gCAACCGCGATAGAGAATCCGGTATAACCCGCCATCTTAATTGCTGCGAAAATCGAACCGACCAGCAGCGCCACTTCCATCAGCATATTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACaaa                                                                                                                                                                                                                                    <  1:432933/251‑1 (MQ=255)                   gATAGAGAATCCGGTATAACCCGCCATCTTAATTGCTGCGAAAATCGAACCGACCAGCAGCGCCACTTCCATCAGCATATTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGc                                                                                                                                                                                                                            >  1:33607/1‑251 (MQ=255)                                  ataACCCGCCATCTTAATTGCTGCGAAAATAGAACCGACCAGCAGCGCCACTTCCATCAGCATATTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTACAACCATCGGATTCGGCGAGAAATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATacc                                                                                                                                                                                                             >  2:504005/1‑250 (MQ=255)                                      cccGCCATCTTAATTGCTGCGAAAATCGCACCGACCAGCAGCGCCACTTCCATCAGCATATTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACAcc                                                                                                                                                                                                         >  1:142245/1‑251 (MQ=255)                                      cccGCCATCTTAATTGCTGCGAAAATCGAACCGACCAGCAGCGCCACTTCCATCAGCATATTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACAcc                                                                                                                                                                                                         >  1:383003/1‑251 (MQ=255)                                         gCCATCTTAATTGCTGCGAAAATCGAACCGACCAGCAGCGCCACTTCCATCAGCATATTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCCAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAACCAAAGGAAAAGCCCGCAACTGCATACACACCCGt                                                                                                                                                                                                      >  2:448770/1‑251 (MQ=255)                                                taagttcatcgaaatccgaccagtccAGCCGCGCCATGTCCATCAGCATAATCATGGCGTTCCGGTTGTTCACCGTCGCATTACGCACCTACGGGTAGCGTCCCAGACGTTTGCCGACCGCACGAGGCAGCAGGACTGCCTCCGAAATACCCCCTGCGCCTAATTCCGCTGCAACGATCTGATTCGGCGAGAGATAGCCCTCCGCATAAACAAAGGTAAAGCCCGCAACTGGATAGACACCCGTTGCTaa                                                                                                                                                                                                <  2:107740/226‑1 (MQ=255)                                                         cGAAAATCGAACCGACCAGCAGCGCCACTTCCATCAGCATATTCATAGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACCAAGGTAAAGCCCGCAACTGCATACACACACGTTGCTAACGCgggggcg                                                                                                                                                                                      >  2:269240/1‑246 (MQ=255)                                                            aaaTCGAACCGACCAGCAGCGCCACTTCCATCAGCATATTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGc                                                                                                                                                                                    >  1:296025/1‑250 (MQ=255)                                                            aaaTCGAACCGACCAGCAGCGCCACTTCCATCAGCATATTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCa                                                                                                                                                                                   <  1:12675/251‑1 (MQ=255)                                                              aTCGAACCGACCAGCAGCGCCCCTTCCATCAGCATATTAATGGCGTTACGGATGTTATCCGACGCATTACGCACCTACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGAAAGACTTCAGCCGAAATAACCACTGCGCCTAATACCGCTACAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACACAGGGAACACCCGCACCTGCATACCCACCAGTTCCTAACGCGGTGGTGGCAAt                                                                                                                                                                                 >  2:536340/1‑251 (MQ=255)                                                                cGAACCGACCAGCAGAGCCACTTCCAACAGCATATTCATGGCGTTACGGATGTTATCCGACGCCTTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCCAAACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGCGCTAGCCCACCGCATAAACCAAGGGAAAGCCCGACACTGCATACACCACCATTGCTAAccagtggggggcaatc                                                                                                                                                                                >  2:480744/1‑235 (MQ=255)                                                                  aaCCGACCAGCAGCGCCACTTCCATCAGCATATTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCAATGCGCCTAATACCGCTGCAACAATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGAAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGGGGTGGCAATCAAc                                                                                                                                                                             >  2:478757/1‑251 (MQ=255)                                                                         cagcagCGCCACTTCCATCAGCATATTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCAGCCGCAATAACCACTGCGCCTAAAACCGCTGCACCCATCGGATTCGGCGAGAGCTAGCCCACCGCATAACAAAAGGTAAAGCCCGCCACTGCATACAAACCCGTTGATAAAGCGGGGGTGGCCATCAACgaccca                                                                                                                                                                       >  2:45417/1‑245 (MQ=255)                                                                          tgcagcGCCACTTCCATCAGCATATTCATGGCGTTACGGACGTCATCCGACGCATTACGCACCTACGGGAAGAGTCCCAGCCCTTTGCCGATCGCACTAAGCAGCCAGACTTCCGACGAAATAAACACTGAGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAACCAACGGTAAAACCCGCGACTGCAAACCCGCCCGATGCTAACGCGggggtgactagcaccagcacaa                                                                                                                                                                      >  2:119826/2‑234 (MQ=255)                                                                          agcagcGCCACTTCCATCAGCATATTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACaa                                                                                                                                                                      >  1:140759/1‑250 (MQ=255)                                                                                             gCATATTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAAt                                                                                                                                                  >  1:225781/1‑251 (MQ=255)                                                                                                tcttaaTGGCGTTACGGATGTTATCCGACGCATTACGCACCGCCGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGAAGCAAGACTTCCGCCGCAATACCCCCTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAAc                                                                                                                                                                                                                                       >  2:83118/6‑163 (MQ=255)                                                                                                tatccATGGAGTTACGGATGTTATCCGACGCATTACGCACCGCAGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCAATGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCACACAACTGCATAACCACCCGTCGCTAACACGGGGGTGGCAATCCCCAGTACAAACCCCAATCCGAGCAtcaagtct                                                                                                                                               >  2:378714/5‑244 (MQ=255)                                                                                                tatTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAAc                                                                                                                                                                                                                                       <  1:83118/163‑1 (MQ=255)                                                                                                 atTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGCGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACCAACCCCAGTCCGCGCATAAATTCTg                                                                                                                                              >  1:7540/1‑251 (MQ=255)                                                                                                         cgtaaCGGATGTTACCCGAGGCTTTACGCACCAACGTGTGGCGCCCCAGCCATTTGCCGCTCGAACGAAGCAGCAAGCCTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTTGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAgcc                                                                                                                                      <  2:229681/247‑1 (MQ=255)                                                                                                                gATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCCTTTGCCGCTCGAACGAAGCAGCAAGACTTCCGCCGCAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAAAGGCACAAACCCCAGACCGCGCATAAATTCTGCCAGAGCCGCCtagc                                                                                                                               >  2:30382/1‑248 (MQ=255)                                                                                                                gATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCt                                                                                                                                  <  1:30382/248‑1 (MQ=255)                                                                                                                       tCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAg                                                                                                                                                                                                                                   <  1:254656/144‑1 (MQ=255)                                                                                                                       tCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAg                                                                                                                                                                                                                                   >  2:254656/1‑144 (MQ=255)                                                                                                                        ccGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACAACCCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGc                                                                                                                       >  1:11293/1‑251 (MQ=255)                                                                                                                          gACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCa                                                                                                                                                                                                                                            >  1:26569/1‑132 (MQ=255)                                                                                                                          gACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTGCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCa                                                                                                                                                                                                                                            <  2:26569/132‑1 (MQ=255)                                                                                                                           aCGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGtt                                                                                                                    <  1:315770/251‑1 (MQ=255)                                                                                                                                  aCGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGAtt                                                                                                             <  1:420192/251‑1 (MQ=255)                                                                                                                                    gCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGt                                                                                                           <  1:327816/251‑1 (MQ=255)                                                                                                                                     ccccGACGGGTAGCGCCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTGCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATCCACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCATTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGtt                                                                                                          <  2:296025/249‑1 (MQ=255)                                                                                                                                                  cGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGcc                                                                                             >  1:41306/1‑251 (MQ=255)                                                                                                                                                     cccAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTg                                                                                           <  1:185427/250‑1 (MQ=255)                                                                                                                                                                gCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCtt                                                                               <  1:455394/251‑1 (MQ=255)                                                                                                                                                                        aacgaaTCAGCAAGACTTCCACCGAAATAACCACTGCGCCTAATACCGCTGCCACCAACGGATTCGGCGAGAGAAAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGAATACACACCCGTTGCTAACGCGGAGGTGGCAATCAACGACCCAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTCATCAGCGTTTGCGCTTGTTCCTGCGTTACGCCTGCGGAAAATGATTTCTCAAgt                                                                       >  2:452300/1‑251 (MQ=255)                                                                                                                                                                            aaGCAGCAAGACTTCCGCCGAAATATCCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGa                                                                   <  1:533235/251‑1 (MQ=255)                                                                                                                                                                                agcaAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGa                                                                   >  1:454261/1‑247 (MQ=255)                                                                                                                                                                                  caAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGa                                                                   >  1:328425/1‑245 (MQ=255)                                                                                                                                                                                        ttCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGt                                                                                                           >  1:566708/1‑199 (MQ=255)                                                                                                                                                                                        ttCCGCCGAAAGAACCACTGCGCCGAATACCGCTGCAACCATCTGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGt                                                                                                           <  2:566708/199‑1 (MQ=255)                                                                                                                                                                                              cgAAATAACCACTGCGACTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACCACGGTAAAGCCCGCAACTGCCTACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAACCCCCAGTCCGCGCATACATTATGCCAGAGACACCTGATTAATCAGCGTTTGCGATTGTTCCGGCGGTACGCCTGCGGAATATGCTTTCTccgttgggcagatcgaccagtaactgc                                                 >  2:336105/1‑226 (MQ=255)                                                                                                                                                                                                 aaTAACCACTGCGCCTAATCCCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAg                                              <  2:140759/251‑1 (MQ=255)                                                                                                                                                                                                                      cGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCa                          >  1:349645/1‑250 (MQ=255)                                                                                                                                                                                                                                aTCGGATCCGGCGAGGGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGTTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTGTGCGATGGTTCCGGCGTTACGCCTGCGGAATATGCTTGCTCCAGTGTGAAGATCGACACTTCACTGGAAGCAAAAATCTTCATGCTGGCAACGGCGGcaat               <  1:125210/251‑1 (MQ=255)                                                                                                                                                                                                                                       tCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGACCGCAACTGCATACACACCCGTTGCTAACGCGGTGGAGGCAATCAACGGCACAACCCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGGTTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGGTCGACACTTCACTGCCAGCAACAATCTTCATGCTGGCAACGGCGGCAATCAATGcc        >  2:54402/1‑250 (MQ=255)                                                                                                                                                                                                                                           cGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTACGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATATTCATGCTGGCAACGGCGGCAATCAATGCTccc     >  1:372293/1‑250 (MQ=255)                                                                                                                                                                                                                                             agagaTAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTCCCACg  >  1:122687/1‑251 (MQ=255)                                                                                                                                                                                                                                             agagaTAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTGCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTCCCACg  <  2:194401/251‑1 (MQ=255)                                                                                                                                                                                                                                             agagaAAGCCCACCGCATAAACAACGGTAAAGCCCGCAACTGCATACACACCCGTTGCTATCACGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGAATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATGGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGGGAAGATCGACACTTCACTGCCAGCAAAAATCTTCCTGCTGGCAACGGCGGCAACCAAGTCTCCCAcc  >  2:218089/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   GAAGTAAATGGCAACCGCGATAGAGAATCCGGTATAACCCGCCATCTTAATTGCTGCGAAAATCGAACCGACCAGCAGCGCCACTTCCATCAGCATATTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGTCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTCCCACG  >  NC_000913/3506149‑3506635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑