Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,388,041	T→G	T50P (ACG→CCG)	aaeB ←	aromatic carboxylic acid efflux pump subunit AaeB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,388,041	0	T	G	100.0%	202.6 / NA	66	T50P (ACG→CCG)	aaeB	aromatic carboxylic acid efflux pump subunit AaeB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (25/41);  total (25/41)

CAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTA  >  NC_000913/3387802‑3388284                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     cAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCCATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGcaa                                                                                                                                                                                                                                           <  1:265165/251‑1 (MQ=255) cAGCGCGGGATAACCGGCCAGCCCCCACGCTAACGAGTTGTCTATTCGAACCAGCGAGGATATCCAGGTACAAAACCCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAAGGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCTCAGCAAAGGCCGGCCcggcgtaa                                                                                                                                                                                                                                            <  2:404961/250‑4 (MQ=255)       ggTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATCCGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATcc                                                                                                                                                                                                                                     >  1:453618/1‑250 (MQ=255)              ccGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGCGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTAACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGt                                                                                                                                                                                                                              >  1:444071/1‑251 (MQ=255)                     gCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTAAAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGAGGCAACAACCGCCGCTGTCAgaac                                                                                                                                                                                                                        >  1:417060/1‑247 (MQ=255)                       ccccACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCCGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCAGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCACAAGCAAAGGCCGGACGGGCGGCAACAATCACCACTGTCAGTACCGc                                                                                                                                                                                                                     >  2:358617/1‑251 (MQ=255)                        cccACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATAGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGAGCCGATGATGCGCAAAAAGCCACGCTCGCGCATAGCCCCAGAATACGGTTCACCTCCCGCCACAAAGACCGGACCGGCGGCAACACACGCCGCTTTCAGTAcatac                                                                                                                                                                                                                    >  2:287739/1‑247 (MQ=255)                        cccACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTAAAAAAACCGGCACAGATACAGCACACCAGAATCATCGATAATGGAGCGGGGATCATCGAAATGATGATCAACAGGCCGGCAGTACAGCCAATAAGTGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATGCGGTTCACCTCCCGCAGCAAAAGCCGGACCGGGGGCAACAATCGCCGCTGTCAGTAcagg                                                                                                                                                                                                                     >  1:362391/1‑247 (MQ=255)                         ccACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGccc                                                                                                                                                                                                                   <  1:351997/251‑1 (MQ=255)                                      gtttCTATTCTTACCAGCGAGGAAATCCAGGTACAAAACCCGGCCCAGATACAGCACACCAGAATCACCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGttt                                                                                                                                                                                                       <  2:522642/249‑1 (MQ=255)                                       tttCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTc                                                                                                                                                                                                      <  1:166618/250‑1 (MQ=255)                                         tatatacGTACCACAGAGGATCTCCAGATACAAAAACCGGCCCAGATACATCACACCAGAATAACCAATAAGGGGGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAAGAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGTCAACAATCGCCGCTGGCAGTACCGCCCAGCGTGGCGTTTCCa                                                                                                                                                                                                    <  2:44937/244‑1 (MQ=255)                                            aTTCGTACCCGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAAGCATCAATAAGGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCa                                                                                                                                                                                                    <  1:58291/247‑1 (MQ=255)                                            aTTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCACTACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAAAAATCGCCGCTGGCAGGACCGCCCaacggggggattcccaga                                                                                                                                                                                                 >  2:58291/1‑233 (MQ=255)                                               cGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGa                                                                                                                                                                                              >  1:409200/1‑250 (MQ=255)                                                gTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGa                                                                                                                                                                                              >  1:134788/1‑249 (MQ=255)                                                     aGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACATCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTATCAGTACCGCCCAGCGCGGGGtttaccgcttgcagtgaa                                                                                                                                                                                        >  2:538065/1‑235 (MQ=255)                                                      acggggATATCCAGGTACAAAAACCGGCCCTGAGACAGCTCACCAGAATCATCAATAATGGTGCGCGGACCATCGCAATGATTATAATAAGTCCGGCAATACAGCCAATAAATGTGCCGAGGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCCCCTCCCGCAGCAAAGGCCGGACCGGCTGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAg                                                                                                                                                                                      <  2:472804/247‑1 (MQ=255)                                                          gtatatCCATGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCTGATCATCGCAATGATGATCCCCTGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCTCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCcaa                                                                                                                                                                                  <  1:522591/249‑1 (MQ=255)                                                            atatCCAGGTACAACACCCGGCCCCGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTACAGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAACGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCCGGACCCCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGGcaac                                                                                                                                                                                 >  2:343575/1‑250 (MQ=255)                                                                  aGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAgc                                                                                                                                                                          >  1:273548/1‑251 (MQ=255)                                                                           aaCCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAACGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCCGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTAc                                                                                                                                                                  >  2:463069/1‑250 (MQ=255)                                                                            aCCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACaa                                                                                                                                                                <  1:456650/251‑1 (MQ=255)                                                                             ccGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGGTCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAAt                                                                                                                                                               <  1:54008/251‑1 (MQ=255)                                                                                 cccAGATACTGCACACCAGAACCATCAATAATGGTGCGCGGATCATCGCATTGACGATCACCAGCCCGGAAAAACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCTGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATTGCg                                                                                                                                                           <  2:458742/251‑1 (MQ=255)                                                                                                ccAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTAcc                                                                                                                                            <  1:563094/251‑1 (MQ=255)                                                                                                 cAGAATCATCAAGAATGGTCCGCGGATTACCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTGTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCg                                                                                                                                           <  2:386680/251‑1 (MQ=255)                                                                                                        atcaATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCg                                                                                                                                    <  1:226683/251‑1 (MQ=255)                                                                                                           aataatGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAat                                                                                                                                 <  1:50635/251‑1 (MQ=255)                                                                                                                    gcgcGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAAAAGCGCCAGTACAATGGCGGTCGCCCGTTTTACCCCaaaaagaaactgttggtt                                                                                                                        >  1:353542/1‑236 (MQ=255)                                                                                                                        ggATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGGTTTACCGCAAAAAGAATAT‑GTTGGGTAGc                                                                                                                    >  1:250120/1‑250 (MQ=255)                                                                                                                          atcatcGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGGCGCCAGATTTAACGCCAAGCGAATAT‑GTTGGGTAgaaa                                                                                                                  >  1:430326/1‑247 (MQ=255)                                                                                                                            catcGAAATGACGCTCACCAGTACGGGAATACAGCCAATAAATGTGCCGAGGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCTCAAAGCGAATAT‑GTTGGTTAGCAATg                                                                                                                <  2:136194/250‑1 (MQ=255)                                                                                                                                       tgatCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCCGTTTTACCGCCAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATAccc                                                                                                    >  1:434899/1‑251 (MQ=255)                                                                                                                                        gaccatcAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCa                                                                                                   <  2:281054/245‑1 (MQ=255)                                                                                                                                         atCACCAGTCCGGCAATACATCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCAt                                                                                                  <  1:540815/251‑1 (MQ=255)                                                                                                                                         atCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACACCCAAGCGGATAT‑GTTGGTTAGCAATGGGGGAAATAcccc                                                                                                   >  1:239788/1‑249 (MQ=255)                                                                                                                                            aCCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATTGTTG‑TTTGCCATGGGGAAAAAACCCCTCGt                                                                                               >  1:103463/1‑251 (MQ=255)                                                                                                                                            aCCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCATCGt                                                                                               <  1:302836/251‑1 (MQ=255)                                                                                                                                            aCCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAAGACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCATCGt                                                                                               <  1:380607/251‑1 (MQ=255)                                                                                                                                                  ccGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCATCGTGATTaa                                                                                         <  1:343575/251‑1 (MQ=255)                                                                                                                                                   cGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCCAAGCGGATAT‑GTTGGGTAGCACAGGGGGAAATACCCATCGTGGTTaa                                                                                         >  1:18439/1‑250 (MQ=255)                                                                                                                                                        aTACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAAc                                                                                   <  1:347352/251‑1 (MQ=255)                                                                                                                                                           cAGCCAATAAATGGGCCGTGGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGTCGGTCGCCAGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCa                                                                                <  2:31061/251‑1 (MQ=255)                                                                                                                                                              ccAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGc                                                                             <  2:224742/251‑1 (MQ=255)                                                                                                                                                               cAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGc                                                                             >  1:221133/1‑250 (MQ=255)                                                                                                                                                                 aTAAAAGTGCCGATGATGCCCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAgg                                                                          <  2:134788/251‑1 (MQ=255)                                                                                                                                                                                atgCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATAT‑GGTGGGTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCggtgtggcactttt                                                           >  1:102415/1‑238 (MQ=255)                                                                                                                                                                                 ggcgcAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTAc                                                          <  1:255905/250‑1 (MQ=255)                                                                                                                                                                                  gcgcAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCaaa                                                                                                                                       <  1:324046/174‑1 (MQ=255)                                                                                                                                                                                   cgcAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCATCGTTATTAGCCAAACCCACGCAGGCGATGGGCCATTTTACgg                                                        <  1:162079/251‑1 (MQ=255)                                                                                                                                                                                    gcAAACAGCCACGAGAGCGAATAACTCCAGAATCCGGTTCACCTCCCTCAGCAAAGGGCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAACGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGga                                                       <  2:193361/251‑1 (MQ=255)                                                                                                                                                                                      aaaaaGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCATGCGATGGGCCATTTTACGgaag                                                     <  1:569754/251‑1 (MQ=255)                                                                                                                                                                                                     cGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGGTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGGACAATGGCGGTAGCCAGATTTACCGCAAAGGGGATAT‑GGTGGGTAGCAAAGGAGAAAAAACCCAACGTGGTTAACCAAAACCACGCAGGCGAGGGGCAATTTTACGGAAGAACAAAACCGGGCtt                                      >  1:299065/1‑251 (MQ=255)                                                                                                                                                                                                      gAATAGCGCCAGAATACGGTTCCCCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCttt                                     <  1:538087/251‑1 (MQ=255)                                                                                                                                                                                                       aaatgctcctgAATACGGTTCACCTCCCGCAGCAAAGGCCGGCCCGGCGGCAACAAACGCCGCTGTAAGGCCCTCCCAGAGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAAGGGCGGTCGCCAGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAAGGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCttta                                    <  2:453618/241‑2 (MQ=255)                                                                                                                                                                                                         aaGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAAGCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTGCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACACATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTtcgt                                  <  2:250120/250‑1 (MQ=255)                                                                                                                                                                                                           gcgcCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTtcgtcg                                <  1:287739/251‑1 (MQ=255)                                                                                                                                                                                                           gcgcCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTtcgtcg                                <  1:499212/251‑1 (MQ=255)                                                                                                                                                                                                               cAGAAGACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGAt                            <  1:538065/251‑1 (MQ=255)                                                                                                                                                                                                                   aTACGGGTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTg                        <  1:20792/251‑1 (MQ=255)                                                                                                                                                                                                                           cgcccccaccagcaAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATAC‑GTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAATCTCTCGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTTGCTTTCGTCGCGATCTTGTTTGCCa                 <  2:444071/241‑1 (MQ=255)                                                                                                                                                                                                                              cTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGAAGGGCCATTTTACGGAAGAACGAAACCTGGCTTTCATCCCGATCTTGTTTGCCAGTg              >  1:363339/1‑250 (MQ=255)                                                                                                                                                                                                                                     agcaAAGGCCGGCCCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACcaccac      <  1:211982/251‑1 (MQ=255)                                                                                                                                                                                                                                        aaaGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCCGTGTTACCGACAAGCGAATGT‑GTTGGTTGGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCCCGCAGGCGATGGGCAATTTTCCCGAAGAACACATCCTGGCTTTCGGCGCGTTCTTGttttcccatagccacccctgg   >  1:342902/1‑233 (MQ=255)                                                                                                                                                                                                                                         aatgCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACATCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTGGTTTGCCAGTGACCACCACTGTa  <  2:273548/248‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     CAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTA  >  NC_000913/3387802‑3388284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑