Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,259,283	G→A	H123Y (CAT→TAT)	tdcG ←	L‑serine deaminase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,259,283	0	G	A	100.0%	183.2 / NA	58	H123Y (CAT→TAT)	tdcG	L‑serine deaminase III
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/30);  total (28/30)

GCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGA‑TATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCT‑ATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTGCCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGC  >  NC_000913/3259047‑3259522                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                 gctgcGCGGCGCTAGCTTAGTGTTCGTCATCAGAC‑AGAATATATAGAGTCCGTTGTAATCACACATTGTCAGCAGTTGTCCGGCTGAGTGGAAATCGGAAGGTACGGCCGTCTCGACATCGGGCGACAGGCCGAAGTGTTCTTCTTCGACATTAACCCCGCCGCCGACAGAGTAATAGGTGTTACTTAATAGCTCTTACTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAActtt                                                                                                                                                                                                                                     <  2:163202/250‑4 (MQ=255) gctgcGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGA‑TATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACAttt                                                                                                                                                                                                                                    <  1:138950/251‑1 (MQ=255)       cAGCGCTAGCTCGTTGTGCATCATCAGACCAGA‑TATAGACAGGCCGATGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGCCGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGCCAGAGTAATAGGATTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGAACCGCATTCCGATCTCATAGCGAGGCAACATTGCGGGAt                                                                                                                                                                                                                              >  2:383375/1‑251 (MQ=255)             tAGCTCGTTGTGCATCATCAGACCAGA‑TATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATggc                                                                                                                                                                                                                           >  1:106959/1‑247 (MQ=255)               gcccgttgttcaacaccaaaCCAAA‑TATAGACAGGCCGTTGTAATCACCCATTTACAGCAGTTCACCGGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATa                                                                                                                                                                                                                      <  2:180174/232‑1 (MQ=255)                     tgtgCATCATCAGACCAGA‑TATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTc                                                                                                                                                                                                                >  1:4525/1‑251 (MQ=255)                           acatcaGACAAGA‑AATAGACAGGCCGTTGGAAGCCCAAATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTTCGAAAGGCCGAAGTTTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCt                                                                                                                                                                                                          <  2:161260/250‑1 (MQ=255)                               ccgACCAGA‑TATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGCTTCGACATCGTGCGACAGGCCGAAGGGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTAc                                                                                                                                                                                                        >  1:111473/3‑249 (MQ=255)                                         tataGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACCGGCCGAAGTGTTCTTCTTCCACAATAAACCCGCCGCCGCCAGAGTAATAGGATTTACTTAATAGCTCTACCTGTCCCTTCCAGGCCGTGATCCGCATTCCGTTCTCATAGCGAGGCATCATTTCGGGATGGCAAATCAAGTTCTTTGATACAGGAAAAtaa                                                                                                                                                                                              >  2:180834/1‑248 (MQ=255)                                                   ccggtGTAATCAAACATTTTCAGCAGTTCACCTGCTGAGTGGACATCGTAAGGTACGGACGTTTCGACATCGTGCGCCAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGAAAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGATCTTTGCTACAGGAAAATCAACAATATgcgc                                                                                                                                                                                   >  2:22229/5‑251 (MQ=255)                                                      ttGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCAcc                                                                                                                                                                                >  2:110622/1‑251 (MQ=255)                                                           aTCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGGCATCGTGCGACAGGCCGAAGTGTTATTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATg                                                                                                                                                                           >  1:374637/1‑251 (MQ=255)                                                                  aTTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTgg                                                                                                                                                                    <  1:387832/251‑1 (MQ=255)                                                                           agTTCCCCTGCTGAGGGGAAATCGTAAGGACCGTACGTGTCGACAGCGTGTGACAGGCCGAGTTGTTTTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCGTTGCTACAGTAAAATCAACAATATGCGCACCAGATGCCACTGGCAGccgccc                                                                                                                                                           <  2:445322/251‑1 (MQ=255)                                                                            gTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCCGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGATAGATAATGTTCTTTGCTACAGGCAATTCAACAATATGCGCACCAGATGCCACTGGCAggcgcacc                                                                                                                                                          >  2:187617/1‑248 (MQ=255)                                                                             ttCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGccgcccgc                                                                                                                                                         >  1:374581/1‑251 (MQ=255)                                                                                    gctgAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTgcgc                                                                                                                                                    <  1:188279/249‑1 (MQ=255)                                                                                     ctgAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGTGCGtt                                                                                                                                                 >  2:122888/1‑251 (MQ=255)                                                                                       gAGTGGAAATCGTAAGGTACGGACGTTTCTACTGCGTGCGACAGGCCGAAGTGTTCTTCTGCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTa                                                                                                                                                <  1:259942/250‑1 (MQ=255)                                                                                         gTGGAAGTCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTGCTTCGACAATAACCCCGCCGCAGACAGAGTAATAGGTTTTACTTAATAGCTTTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTAATAGCGAGGCAACATTTCGGGATGGAAGGGATGGTTCTTAGCTCCAGGAAAATAAACAATATGAGCACCAGATGCGAATGGCATCCGCCGGCTGCGCGATAca                                                                                                                                              >  2:426438/1‑249 (MQ=255)                                                                                         gTGGAAAGCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCAGAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTa                                                                                                                                             <  1:367076/251‑1 (MQ=255)                                                                                                 cGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCATATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCt‑at                                                                                                                                     <  1:473905/250‑1 (MQ=255)                                                                                                  gTAAGGTACGGCCGTTTCGACATCGGGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTACAGGCAGTGATCCGCCTTCCGTTCTCATAGCGAGGCACCATTTCGGGATGGAAGCTAATGTTCTTTGCAACAGGAAAATAAACAATATGCGCACCAGATACCCCTGACAACCGCCCCCTGCGCTTTACAAACTCt‑ata                                                                                                                                    >  2:350318/1‑250 (MQ=255)                                                                                                           ggACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCT‑ATAAATGCAGGGa                                                                                                                          <  1:191328/251‑1 (MQ=255)                                                                                                             aCGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCT‑ATAAATGCAGGGAtc                                                                                                                        >  1:432198/1‑251 (MQ=255)                                                                                                               gttccaacatctggcaccagtccGAAGTGTTCTTCTCAGCCATTAACCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGTTCCGCATTCCGTTCTCATAGCGATGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCAGTGGCAGCCGCCCGCTGCGCGTTACTACCTCT‑ATAAATGCAGGGAtctc                                                                                                                      <  2:111473/230‑1 (MQ=255)                                                                                                               ggtgcGACACCGTGCGACAGGCCGAAGTGTTCTTATTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATg                                                                                                                                                                                      <  2:124773/184‑1 (MQ=255)                                                                                                               gTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATg                                                                                                                                                                                      >  1:124773/1‑188 (MQ=255)                                                                                                                tttCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGATCTTTGCTACAGGAAAATCAACAATATGCGCACCAAATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCT‑ATAAATGCAGGGAtctc                                                                                                                      >  1:239126/1‑250 (MQ=255)                                                                                                                      catcgtaagACAGGACGACGTGTACTTATTCGACACTAAACCCGCCGCCGACAGAGTAATAGGTTTTAATTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCAGCATGCCGTTATCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACCGGAAAATAAACAATATGCGCACCAGATGCCACTGGCATACACCCGCTGCACGTTACTAACTCT‑ATTACTGTAGGAATCCCATCAATg                                                                                                               >  2:223225/9‑251 (MQ=255)                                                                                                                      cATCGTGCGACATGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCT‑ATAAATGCAGGGATCTCATCAATg                                                                                                               <  1:137444/251‑1 (MQ=255)                                                                                                                             cggcAGGCCGAAGGGTTCTTCTTCTACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTTTCCCTTCCAGGCAGTTATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCTCCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACACT‑ATAAAGGCAGGTATCTCAGCATTGACAACAt                                                                                                        <  2:528710/248‑1 (MQ=255)                                                                                                                              gACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCT‑ATAAATGCAGGGATCTCATCAATGACAACATc                                                                                                       <  1:122888/251‑1 (MQ=255)                                                                                                                                   ccagaattggtcttcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATACGCATTCCGTTCTCATAGCGAAGCAACATTTCTGGATGGAAGATAATGTTCTTTGCTACAGTAAAATCAACAATATGCGCAACAGATGCCACTGGCATCCGCCCGCTGCGCTTTACTAACTCT‑ATAAATGCAGGGATCTCATTAATGACAACATCCTGCg                                                                                                  <  2:249535/241‑1 (MQ=255)                                                                                                                                         gtgtTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCT‑ATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGt                                                                                            <  1:15860/251‑1 (MQ=255)                                                                                                                                           gtTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCT‑ATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGttt                                                                                          <  1:24610/251‑1 (MQ=255)                                                                                                                                                       cAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCT‑ATAAATGCAGGGATCTCTTCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTccc                                                                              >  1:175357/1‑251 (MQ=255)                                                                                                                                                               ccgccgtcgGCAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATAAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCT‑CTAAATGCAGGACTCTCATCAAAGACAACATCCTTCAGACTGTTTCCTGCCACTccacatcgtat                                                                      >  2:503300/1‑242 (MQ=255)                                                                                                                                                                  ccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCT‑ATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGc                                                                   <  1:302273/251‑1 (MQ=255)                                                                                                                                                                  ccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCT‑ATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGc                                                                   >  1:545121/1‑251 (MQ=255)                                                                                                                                                                    gccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCCGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCT‑ATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATggtg                                                                  >  1:28789/1‑248 (MQ=255)                                                                                                                                                                     ccgACAGAGTTAAAGGGTTTTCTTATAAGCGCTTCGCGTCCCTTCCAGGAAGCGATCCGCATCCCGTTCTCATAGCGAGGCACCATTTCGGGTTGGAAGATAATGTTCTTTGCTCCAGGAAAATCAACACTATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTGACTAACTCT‑ATAAATGCAGGGCTCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGAc                                                                <  2:134920/251‑1 (MQ=255)                                                                                                                                                                         cAGAGAAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGTGCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCT‑ATAAAGGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATcc                                                            <  2:246252/251‑1 (MQ=255)                                                                                                                                                                                 aaGGTTTTACTTAATACCCTTTCATGTCCCTTCCAGGCAGTAATCCTCATTCCGTTATCATAGCGAGTGAACATTACGGGATGGAAGATAATGTTCTTTTCTACAGAAAAACCAACTATATGCCCATCAGATGCCTCTGCCAGCCGCCCGCTGCGCGTTACTAA‑TCTTATAAATGCAGGGATCTCAGCAATGCCAACATCCTCCGCACTGTTTCCTGACAGTCCCATGATGATGGCGACATCCGTGGCATg                                                    <  2:435429/250‑1 (MQ=255)                                                                                                                                                                                  aGGTTTTCTTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATATCGGGATGGAAGATAATGTTCTTTGCGTCAGTAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCT‑ATAAATGCAGGGATCTCATCAATGTCAACATCCTGCGGACTGTTTCCTGCCATTCCCAGGATGATGGCGACATCCGTGGCATgg                                                   <  2:529260/251‑1 (MQ=255)                                                                                                                                                                                          tttAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCTGCCTCCCGCTCCGCGTTACTAAATCT‑ATAAATGCCGGGATCTCATCAATGCCAACATCATGCGAACTGTTTCCTACCCGTCCCATGTTGACGGCGTCATCCGTGGCATGGCCTTTGcc                                           >  2:418846/2‑251 (MQ=255)                                                                                                                                                                                                aGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCT‑ATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTGCCCGTCAg                                     <  1:22229/251‑1 (MQ=255)                                                                                                                                                                                                  ctctTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCT‑ATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGCTGATGGCGACATCCGTGGCATGGCCTTTGCCCGTCAGTg                                   >  2:214087/1‑251 (MQ=255)                                                                                                                                                                                                      tCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCT‑ATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTGCCCGTCAGTGACa                                >  2:197882/1‑250 (MQ=255)                                                                                                                                                                                                      tCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCT‑ATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTGCCCGTCAGTGACa                                >  1:106272/1‑250 (MQ=255)                                                                                                                                                                                                          cacccTTCCAGGCATTGACCCTCATCCCGGTCTCATAGCGAGACAACATTTCGGGATGGAAGAAAATTTTCTTTATCACAGGAAAATCAACTATAGGCGCGCAAGAGGCCCCTGGCAGCGGCCCGCTGCGCGTTACTAACTCT‑ATAAAGTCAGGGATCTCATCAATGACATCACCCTGCGGATTGTTTCCTGCCAGTCCCATGATGATGTCGACATCCGTGGCATGGCCTTTGCCCGTCAGTGACAACGAc                           <  2:55241/249‑1 (MQ=255)                                                                                                                                                                                                            cccTTCCAGGCAGTGATCCGAATTTCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCCCCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCT‑ATAAATGCAGGGACCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTT‑GCCCGTCAGTGACAACGACCCg                        <  2:93304/251‑1 (MQ=255)                                                                                                                                                                                                                  cAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCT‑ATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTGCCCGTCAGTGACAACGACCCGTACAg                   <  1:308686/251‑1 (MQ=255)                                                                                                                                                                                                                       aGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCT‑ATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTGCCCGTCAGTGACAACGACCCGTACAGATCg               >  1:496694/1‑250 (MQ=255)                                                                                                                                                                                                                          gATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCT‑ATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTGCCCGTCAGTGACAACGACCCGTACAGATCGAcc            <  1:110622/250‑1 (MQ=255)                                                                                                                                                                                                                             ccGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCT‑ATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTGCCCGTCAGTGACAACGACCCGTACAGATCGAcccca         >  1:47271/1‑247 (MQ=255)                                                                                                                                                                                                                                 aTTCCGTTCTCAAAGCGAGGCAACATTTAGGGTTGGAAGTCAATGTTCTTTGCTCCAGGAAAACCAACATAATGCTCACCATATGCCACGGTCAGCCACCCGCTGCGGGTTACTACCTCT‑ATAAATGCAGGTACCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGAGGATGGCGACATCCGTGGCATGGCCTTTTCCCGTCAGTGACAACGACCCGTACAGATCGTCCACAATATg    <  2:374581/251‑1 (MQ=255)                                                                                                                                                                                                                                   tCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGCAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCT‑ATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTGCCCGACCGTGACAACGCCCCGTACAGCTAGCCAACAATATGGc  >  2:382748/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                 GCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGA‑TATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCT‑ATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTGCCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGC  >  NC_000913/3259047‑3259522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑