Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,227,339	C→T	T369I (ACC→ATC)	ygjI →	putative transporter YgjI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,227,339	0	C	T	93.3%	85.5 / NA	30	T369I (ACC→ATC)	ygjI	putative transporter YgjI
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base T (22/6);  total (22/8)
Fisher's exact test for biased strand distribution p-value = 6.44e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCCGATGCTCGGTTCCAATACCGTGCAGGATC‑TGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTCGGCGGTATTGTT  >  NC_000913/3227097‑3227565                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                   cGGTCTGGTGTCCTGTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCCTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCCGATGCTCGGTTCCAATACCGTGCAGGATC‑TGATGAATACTATTATTAATATGATCGCCGCa                                                                                                                                                                                                                             <  1:58888/250‑1 (MQ=255)          gtCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCCGATGCTCGGTTCCAATACCGTGCAGGATC‑TGATGAATACTATTATTAATATGAtcgc                                                                                                                                                                                                                                 >  1:163438/1‑237 (MQ=255)          gtCCTTTACCGCGATGTTCGGTTCCCTGCTGATGGGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCCGATGCTCGGTTCCAATACCGTGCAGGATC‑TGATGAATACTATTATTAATATGAtcgc                                                                                                                                                                                                                                 <  2:163438/237‑1 (MQ=255)             cTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCCGATGCTCGGTTCCAATACCGTGCAGGATC‑TGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTc                                                                                                                                                                                                                >  1:131104/1‑251 (MQ=255)                  ccGCGATGTTCGGTTCCCTGCTGATGTGGCCCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGTTCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCCGATGCTCGGTTCCAATACCGTGCAGGATC‑TGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTccgcc                                                                                                                                                                                                            <  2:462892/250‑1 (MQ=255)                                       tgctgtgGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAAAGGCGTTCCGGCGCGCGAAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCCGATGCTCGGTTCCAATACCGTGCAGGATC‑TGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTGCCGCCGTTATTCATCATGCTTGCTTAc                                                                                                                                                                                      >  2:386162/4‑251 (MQ=255)                                       tgATGTGGACCGCAACGCCGGTGAAACTTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCCGCTGCTCGGTTCCAATACCGTGCAGGATC‑TGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTAc                                                                                                                                                                                      >  1:178069/1‑251 (MQ=255)                                        gATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCCGATGCTCGGTTCCAATACCGTGCAGGATC‑TGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTAc                                                                                                                                                                                      <  1:460110/250‑1 (MQ=255)                                             ggACCGAAACGCCTGTGAAAATTTTCTTCTCCGAAAACCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCCGATGCTCGGTTCCAATACCGTGCAGGATC‑TGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAAt                                                                                                                                                                                <  1:131538/251‑1 (MQ=255)                                                                                          gCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCCGATGCTCGGTTCCAATACCGTGCAGGATC‑TGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGc                                                                                                                                   >  2:212286/1‑251 (MQ=255)                                                                                            aTCTTTGGTAAGCAAACCGTCGAACTGAACGAAACCGGCGTTCCGGCGCGCGCAGAGAGGCTCCAGTTCCTGATCGTCATACCGCTGATGATTAT‑CCCGATGCTCGGTTCCACTACCGTGCAGGATC‑TGTTGAATACTATTATTAATATGATCGCCGCAGCGTCCCTGCTTCCGCCGTTATTCATCATGCTGTCTTACCTGAATTTACGCGCCACATTCGATCACCTGCCACGCGATTTCCGTATGGGCTc                                                                                                                                 >  2:59748/1‑251 (MQ=255)                                                                                            aTCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCCGATGCTCGGTTCCAATACCGTGCAGGATC‑TGCTGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTCTTCATCATGCTGGCTTACCTGAATTTACGCGCCAACTTCGATCACCTGCCACGCGATTTCCGTATGGGCTc                                                                                                                                 >  2:275846/1‑251 (MQ=255)                                                                                                      aGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCCGATGCTCGGTTCCAATACCGTGCAGGATC‑TGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCAcc                                                                                                                       >  2:533822/1‑251 (MQ=255)                                                                                                         aaaCCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCCGATGCTCGGTTCCAATACCGTGCAGGATC‑TGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGt                                                                                                                    >  1:477172/1‑251 (MQ=255)                                                                                                                    cTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCCGATGCTCGGTTCCAATACCGTGCAGGATC‑TGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCgttgt                                                                                                         >  1:170150/1‑251 (MQ=255)                                                                                                                      gAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCCGATGCTCGGTTCCAATACCGTGCAGGATC‑TGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGttt                                                                                                       >  1:468826/1‑251 (MQ=255)                                                                                                                                ggCGTTCCGGCGCGCGCAGCGTGGATCCAGGTCCTGATCGTCATCCCGCTGATGATTAT‑CCCGCTGCTCGGTTCCAATACCGTGCAGGATC‑TGCTGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAACTTAGATCACCTGCCACTCGATTTCCGTATGTGCGCCCGCCGCCCCGGCATTATCGTTGTTTCAATACTGAt                                                                                             >  2:469658/1‑251 (MQ=255)                                                                                                                                     tCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCCGATGCTCGGTTCCAATACCGTGCAGGATC‑TGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGGATTATCGTTGTTTCAATCCTGATTGCgt                                                                                        >  2:286654/1‑250 (MQ=255)                                                                                                                                          tcccaatctggtgaatccatctCCTGATCGTCACCCCGCT‑ATTGTTATTCCAGATCCTCGTTTCAAATCCCGTGCAG‑ATCCAGATGATTCCTTTTATTAATTTGATCGCCGCAGCTCCCTTGCTCCCGCCGTATTTCATCATGCTGGCTTACCTAAATTTCCGCGCAAAATTAGCTCACCTTCCAGGCTTTTTCCTTAGGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATAtt                                                                                    <  1:314010/229‑1 (MQ=255)                                                                                                                                             gcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCCGATGCTCGGTTCCAATACCGTGCAGGATC‑TGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATATTTGcc                                                                                >  1:244789/1‑251 (MQ=255)                                                                                                                                                   cGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCCGATGCTCGGTTCCAATACCGTGCAGGATC‑TGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATAttagattcgga                                                                           >  2:314010/1‑241 (MQ=255)                                                                                                                                                                      cGTCACCCCGTTGATGATTAC‑CCCGCTGCTCGGTTCCAATCCCGTGCAGGATT‑GGATAAATCCTATTATAAATATGACCCCCGCAGCGCCCATGCTCCCGCCGTTATTCATCATGCTGGCTTCCCGGAATTTCCGCGCCAAATTAGATCCCCTCCCACGCGATTTCCGTATGGGCCCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTcc                                                       <  1:533822/251‑1 (MQ=255)                                                                                                                                                                               gCTGATGATTAT‑CCCGATGCTCGGTTCCAATACCGTGCAGGATC‑TGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATATTTTCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGgcg                                              >  2:95447/1‑251 (MQ=255)                                                                                                                                                                                  gatgatTAT‑CCCGATGCTCGGTTCCAATACCGTGCAGGATC‑TGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGcgcg                                            >  1:47996/1‑250 (MQ=255)                                                                                                                                                                                                 tGCTCGGTTCCAATACCGTGCAGGATC‑TGATGACTACTATTATTACTATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACcacc                             >  1:156172/1‑249 (MQ=255)                                                                                                                                                                                                        ttCCAATACCGTGCAGGATC‑TGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCAtttttt                      >  1:139242/1‑251 (MQ=255)                                                                                                                                                                                                           cAATACCGTGCAGGATC‑TGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTtat                    >  1:552875/1‑250 (MQ=255)                                                                                                                                                                                                             aTACCGTGCAGGATC‑TGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGCTTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATCTTCTGACCATCATTTTTTATAAc                 >  1:237638/1‑251 (MQ=255)                                                                                                                                                                                                                   tgcgggATC‑TGATGATTACTATTATTAATATGATCGCCGCAGCGTCCATTCTTCCCCCTTTTTTCATCAGTCTGGCTTACCTGAATTTACGCGCCAAATTAGATCCCCTGCCCCGCGATTTCCGTAGGGGCCCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTcg             <  1:386162/245‑1 (MQ=255)                                                                                                                                                                                                                    gCAGTATC‑TGATTAATATTTTTATAAATATGACCGCCGCAGCTTCCATGCTCCCGCCGTTATTCATCATGCTGCCTTACCTAAATTTACGCGCCAAATTAGATCCCCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTcggcg          <  1:163867/251‑1 (MQ=255)                                                                                                                                                                                                                             tgatgaATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTCGGCGGTAGTGtt  >  2:170890/1‑251 (MQ=255)                                                                                                                                                                                                                              gatgaATACTCTAATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGACTTTACGCGCCAAATGAGATCACCTGCCACGCGATTTCCGTCTGGGCTCCCGCCGCACCGGTATTCTCGTTGTTTCAATGCTTATTGCGATATTTGCCGTCGGGTATGGCGCTTCGACATTCCCGACTGGCGAGAATATTCTGGACACCATTTTTTCTATCGTcggcgagcttgta  >  2:472597/1‑242 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                   CGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCCGATGCTCGGTTCCAATACCGTGCAGGATC‑TGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTCGGCGGTATTGTT  >  NC_000913/3227097‑3227565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑