Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,224,895	C→T	L755L (CTG→TTG)	ebgA →	evolved beta‑D‑galactosidase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,224,895	0	C	T	97.5%	248.7 / NA	83	L755L (CTG→TTG)	ebgA	evolved beta‑D‑galactosidase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (42/37);  minor base G (2/0);  total (46/37)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAACATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCGATGGCCAGGTTA  >  NC_000913/3224651‑3225135                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                 caacacttcGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTAACAGTTCCCGCGGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCTCTGTTGAc                                                                                                                                                                                                                                              <  2:498040/241‑1 (MQ=255) cAACATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTc                                                                                                                                                                                                                                            <  1:475578/251‑1 (MQ=255)     aTTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACTCCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGa                                                                                                                                                                                                                                        <  1:141572/251‑1 (MQ=255)          ggTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCa                                                                                                                                                                                                                                    >  1:209261/1‑250 (MQ=255)             gACCAAAGAGTCCCGCACCCGCTACAGCGAATCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGa                                                                                                                                                                                                                                <  1:517874/251‑1 (MQ=255)                 aaaGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCa                                                                                                                                                                                                                             >  1:340359/1‑250 (MQ=255)                   aGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAAct                                                                                                                                                                                                                          <  1:265022/251‑1 (MQ=255)                        cccGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACttcttc                                                                                                                                                                                                                     >  1:254754/1‑251 (MQ=255)                           ccACCCGCTACAGCGAATCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGGTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGTTGAATGGCGAAGCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAg                                                                                                                                                                                                                  <  2:44056/250‑1 (MQ=255)                             aCCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGcc                                                                                                                                                                                                                >  1:235196/1‑251 (MQ=255)                               ccGCTACAGCGAAGCCGGACCCCCTATCGCCCCTTATCAGTTCCCGCTGAAGGAAAACCCCGCGCAGCCAGTGCCTTTCGCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCga                                                                                                                                                                                                              <  2:544897/251‑1 (MQ=255)                                cGCTACAGCGACGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGAGCAGCCAGTGCCTTTCGCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGCATCGCTGGTGACTCGCGAGCCAAAGATAAAATTCTTACAGCCgat                                                                                                                                                                                                             >  2:410899/1‑251 (MQ=255)                                cGCTACAGCGAAGCCGGCCCCCCTCCCGCCACTTATCAGTCCCCGCTAAAGGAAACCCCCGCGCAGCCAGTGCCTTTCGCACAAAATAAGG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGGGCTG‑CCCCGTTCGCGGCTACACCTTCGCGATCCCCT‑TCTCAAAAATGAGTGGCAAACCGACATCCGGGCAGGGGAATGGCGAACCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCgat                                                                                                                                                                                                             <  1:88838/251‑1 (MQ=255)                                  cTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCgatg                                                                                                                                                                                                            >  1:407810/1‑250 (MQ=255)                                    aCAGCGAAGCCGGACCCCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCgatgat                                                                                                                                                                                                          <  1:173623/250‑1 (MQ=255)                                     cAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCg                                                                                                                                                                                                        <  1:216046/251‑1 (MQ=255)                                           aGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGAACCAAATCATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCAgatgagctaacaac                                                                                                                                                                                                  >  2:258169/1‑242 (MQ=255)                                                gACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAg                                                                                                                                                                                             <  1:110843/251‑1 (MQ=255)                                                  cacCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCCCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGc                                                                                                                                                                                            >  1:255458/1‑250 (MQ=255)                                                   acCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTAGCGGCTACAACTTAGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTATTCAAGCCGATGATCGACAACCACCAGc                                                                                                                                                                                            >  2:462253/1‑249 (MQ=255)                                                        aTCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGAGCAGCCAGTGCCTTTCGCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTAGCAAACCGACATCCTGGCAGGTGAATGGCGAGTCGCTGTTGACTCGCGAGCCAAAGATAAACTTCTTCAAGCCGATGATCGTCAAACACAAGCAGGAGt                                                                                                                                                                                      >  1:453399/1‑250 (MQ=255)                                                          cGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCCCAAGCAGGAGTACg                                                                                                                                                                                   <  1:229810/251‑1 (MQ=255)                                                           gCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCCAAGATCAACTTCTTCAGGCCGATGATCGAAAACCCCAAGCAGGGGTACg                                                                                                                                                                                   >  2:549782/1‑250 (MQ=255)                                                             cACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCCCCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCCAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGa                                                                                                                                                                                  >  1:350312/1‑249 (MQ=255)                                                                  aTCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCtg                                                                                                                                                                           >  1:269899/1‑251 (MQ=255)                                                                     aGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCCCCAAATAATG‑CGCGTACGCTGACG‑CTGGAAGACG‑TTCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGATTGGCGAATCGCTGTTGACTCGCGATCCAAAGATCCACTTCTTAAAGCCGATGATCGACAACCAAAAGCAGGAGTAAGAAGGGCTGTgg                                                                                                                                                                        >  2:521202/1‑251 (MQ=255)                                                                       tccccGCGGAAGGAAAACACCGGGCAGCAAGTTCCTTTCGCACCAATGAATG‑CGCGTCCGCTGACG‑CTGGAAG‑CGTATCGTTTGAGCTG‑CACCGTTCGCGGTTACAACGTCGCGATAACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCAAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGc                                                                                                                                                                       <  2:137458/248‑1 (MQ=255)                                                                         cccGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCCCAAAATAATG‑CGCGTCCGCCGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGAACAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGTAGTACGAAGGGCTGTGGCAAc                                                                                                                                                                    <  1:237428/251‑1 (MQ=255)                                                                          ccgcccttccgatcccccGCGCAGCCAGTGCCTTTCGCACCAAATAATG‑CGCGTCCGCCGACG‑CTGGAAGACG‑ATCGGTTGACCTG‑CACCGTTCGCGGCTCCAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGAGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTGCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAAcc                                                                                                                                                                   <  2:435775/235‑1 (MQ=255)                                                                             cTGACGGAAAACACCGCGCAGACAGTGCCATTCGCACCAAATAATC‑CGAGACCGCTGACC‑CGGGCAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TATCAAAAATGCGTGGCAAACCGACATCCTGGCAGGTGACGGGCGAATCGCTGTTGACTCGCGCACCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACTAGCAGGAGTACGAAGGGCtgtaggcaccga                                                                                                                                                                 >  2:320230/1‑241 (MQ=255)                                                                             cTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGa                                                                                                                                                                 >  1:111960/1‑250 (MQ=255)                                                                              ggAAGGAAATCCCCGCGTAGCACGTGCCTTTCGCACCAAATA‑TGTCGCGCCCGCTTACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAAGGAGTGGCAAACCGACATCCTGGCAGGTGAAGGGCGAATCGCTGTTGACCCGCGAGCAAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAATCAGGAGTACGAAGGGCTGTGTCATCCGAAt                                                                                                                                                               <  2:368770/250‑1 (MQ=255)                                                                               gAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATc                                                                                                                                                              >  1:404496/1‑251 (MQ=255)                                                                                aaGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATc                                                                                                                                                              >  1:117740/1‑250 (MQ=255)                                                                                 agaaaaaCACCGCGCAGCCAGAGCCTTTCGCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTATCAGCTG‑CACCGTTCGCGGCTACAACCTCGCGATCACCT‑TCTCAAAACTGAGTGGCAAACCGACATCCTGGCAGGTGAATTGCGAAGCGCTGTTTACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGCGGCTCGACAACCACAAGAAGGGGTACGAAGGGCcgtgcgaaccggatcct                                                                                                                                                            >  2:16749/4‑238 (MQ=255)                                                                                         acCGCGCAGCCAGTGCCTTTCGCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGAGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAAcc                                                                                                                                                                   >  1:435775/1‑236 (MQ=255)                                                                                            gcccAGCCAGTGTCTTTCGAACCAAACTAGG‑CGCGTCCGCGTCCG‑CTGGATGCCA‑GTCGTCTGACCTG‑CCCCTTTCGCGGCTACAACTCCGCGATCGCCT‑TCGCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTAGACCCGCTAGCCAAAGACCAACTTCTTCAAGCCGATGATCGACAACCTCAAGCAGGAGTACTAAGGGCTGTGGCAACCGACTCATTTGCAGATCAt                                                                                                                                                 <  2:396603/248‑1 (MQ=255)                                                                                             cgccgCCAGGGCCTTTCGCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACTGACATCCGGGAAGGTGAATGGCGAAACGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATg                                                                                                                                                <  2:497380/247‑1 (MQ=255)                                                                                             cgcAGCCAGTGCCTTTCGCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATg                                                                                                                                                >  1:422363/1‑251 (MQ=255)                                                                                             cgcAGCCAGGCCCTTTCGCACCAAATAAGG‑CGCGCCCGCTGACG‑CTGGAAGACG‑ATCTTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATg                                                                                                                                                <  2:117740/251‑1 (MQ=255)                                                                                                 gCCAGTGCCTTTCGCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAgg                                                                                                                                            >  1:47105/1‑251 (MQ=255)                                                                                                 gCCAGTGCCTTTCGCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CAACGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGCGAATGGCGAATCGCTGGTGACTCGCGAGCCAAAGATCAACTTCTTCAATCCGTTGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGCATCATTTGCAGATCAAGCAgg                                                                                                                                            >  2:53363/1‑251 (MQ=255)                                                                                                  ccggggCCTTTCCCACCAAATAATG‑CGCGGCCGCTGACGTCTG‑ATGACG‑ATTGGTTGAG‑TGTCACCGTTCGCGGCTAAAACTTCTCGATCACCT‑TCTCAAAAATGATGGGCAAACCGACAGCCGTGCAGGGGAAGGGCGAATCGCTTTTGACTCGCGAGCCAAAGACCAACTTCGTCAATCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAAGCATTTGCAGATCATGCAGGa                                                                                                                                           <  2:340359/246‑1 (MQ=255)                                                                                                    aGTGCCTTTCGCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAAc                                                                                                                                         <  1:126924/251‑1 (MQ=255)                                                                                                        ccTTTCGCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGACGACG‑ATCGTTTGAGCTG‑CACCGTTAGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATACTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAACTCAACTTCTTCAAGCCGATGAACGACAACCACAAGCCGGAGAACGAAGGGCTGTGGCAACAGAATCCTTTGCAGATCATGCAGGAACATCt                                                                                                                                     >  2:227973/1‑251 (MQ=255)                                                                                                                   aaaTAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTg                                                                                                                          <  1:227973/251‑1 (MQ=255)                                                                                                                             ccggccGCTGCCG‑CGGGAAGACG‑CTTGTTATAGCGG‑CTCCTTTCGCGGCGACATCTTCGCGATCACCG‑TCCCAAAAATGAGTGGCAAACCCACAGTCTGGAAGGTAATTGGCGAATCGCTGTTGCCCCGCGAGCCAAAGACCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTTCCGTAGAAc                                                                                                                 <  2:533275/247‑1 (MQ=255)                                                                                                                               gTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGGGCATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCACCTTCTTCAAGCCGATGTTCGCCAACCACAAGCAGGAGTACGAAAGGCTGTGGCAACAGCATACTTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAAc                                                                                                                 >  2:465573/1‑249 (MQ=255)                                                                                                                                cccGCTGACG‑CTGGAAGACG‑ACCGTTGGGGCTG‑CACCGTTCGCGGCTACAACTGCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTAAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACaga                                                                                                              <  2:196941/250‑1 (MQ=255)                                                                                                                                 ccGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCGCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACaga                                                                                                              <  1:522179/250‑1 (MQ=255)                                                                                                                                  cgctggcg‑ctggAAGACG‑ATCGGTGGAGCGG‑CACCGTCCGCGGCAACAACGACGCGATCCCCC‑TCTCAAAAATGGTTGTCAAACCGACATATGGGCAGGTGAATGGCGAATCGCATTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCTCGATTTTGCCTTAGAACagaga                                                                                                            <  2:350312/251‑2 (MQ=255)                                                                                                                                      gACG‑CTGGAAGACT‑AGCGTTTGAGCTG‑CCCCGTGCGCGGCTACAACTGCGCGATCCCCT‑TCTCAAAAAGGAGTGGCAAACCGACATCCTGGCAGGGGAATTGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATg                                                                                                        <  2:399319/251‑1 (MQ=255)                                                                                                                                            tGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGa                                                                                                    >  2:154120/1‑250 (MQ=255)                                                                                                                                            tGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGa                                                                                                    >  1:402512/1‑250 (MQ=255)                                                                                                                                                 gccG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCGCAAAAATGAGTGGCAAACCGACACCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCt                                                                                              <  1:449072/249‑1 (MQ=255)                                                                                                                                                  aCG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACCGAGCGATGGGGAAGTGCTg                                                                                             >  2:197661/1‑251 (MQ=255)                                                                                                                                                   acgacctttgtagcgg‑CACCGTTCGCGGCTACAACTGCGCGAT‑ACCTGTCTCAAAAATGAGGGGCAAACCGCAATCCTCGCGGGTGAACGGCTATTCGCTGTTGACTCGCGAGCCAAAGAGCAACTGCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCGGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACATAGCGATGGTGAAGTGCTg                                                                                             <  2:541524/236‑1 (MQ=255)                                                                                                                                                           ttGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGAtcatca                                                                                      <  1:405552/250‑1 (MQ=255)                                                                                                                                                                cTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGc                                                                                 >  1:411427/1‑250 (MQ=255)                                                                                                                                                                  g‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGcac                                                                               >  1:283504/1‑250 (MQ=255)                                                                                                                                                                       cGTTCGCTGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCTACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTa                                                                          <  1:519496/251‑1 (MQ=255)                                                                                                                                                                        gttctgggcaccaacttatcaatgaCCT‑TCACAAAAATGAGTGGCAAACCGACAACCTGGCATGTGAATGGCGAATCGCGGTGGACTCGCGAGCCCAAGATCAACTTCTTCAAGCCGAAGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCAGATCATCAGCCGCACATTTAt                                                                         <  2:471376/227‑1 (MQ=255)                                                                                                                                                                          tCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGAAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTg                                                                       >  1:383764/1‑251 (MQ=255)                                                                                                                                                                             cgGCTACAACTTCGCGAAAACAT‑TCTCAAAAATGAGTGGCAAACCGCCATCCTGGCAGGTGAATGGCGAAACGCTGTTGACCCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGCCAACTACAAGCAGGGGTAAGAAGGGGTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTACCGTAGAAAAGAGCGATGGTGAAGTGCTGATCATCACCCCCACAGTTATTGctc                                                                    >  2:261355/1‑249 (MQ=255)                                                                                                                                                                               gctaaatcttctctgtCACCT‑TCTAAAAAATGAGTGGCAAACCGACACCCTGGCAGGTGAATGGCGAATCGCTGTGGACTCGCGAGCAAAAGAACAACTCCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACTAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCTTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGccgcg                                                                  <  2:408124/236‑4 (MQ=255)                                                                                                                                                                                     ccTTCGCGATCACCT‑ACTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAACGGCGCATCGCTGTTGACCCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGAACGAAGGGCTGTGGCAACCGGATCATCTGCAGATCCTGCAGGAACATCTGCGCGACTTTGCCGAAGAAAAGAGCGATGGTGAAGAGCTGCTCATCAACCGCACAGTTATCTCCCCGCCggcg                                                            >  2:259261/2‑249 (MQ=255)                                                                                                                                                                                               cACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGACCAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCg                                                  >  1:418509/1‑251 (MQ=255)                                                                                                                                                                                                aCCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGACGAATCGCTGTTGACTCGCGAGCCCACGATCAACTTCTTCAAACCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGACCCGCCGGTGTTTGACTTCgg                                                 >  1:529379/1‑251 (MQ=255)                                                                                                                                                                                                           aaaTGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCTGGAGTACGAAGGGCTGAGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACa                                                                                                                >  2:18169/1‑178 (MQ=255)                                                                                                                                                                                                           aaaTGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACa                                                                                                                <  1:18169/178‑1 (MQ=255)                                                                                                                                                                                                            attGAGTGGCAAACCGCCACCCTGGAAGTGGAATGGCGAACCGCTGTTGCCTCGCGAGCAAAAGATCAATTTCTTCAAGCCGATGATCGACAACCACAAGAAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCCCAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCa                                      <  1:106180/249‑1 (MQ=255)                                                                                                                                                                                                                      aaaCCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGCTCGACAACCACCAGCAGGAGTACGGAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGACCAGAGCGATGGTGAAGTGCTGCTCATCCGCCGCACAGTTATTGCCACGCCGGTGTTTGACTTCGGGATGCGCAGCACCTCCATCTg                            >  2:227015/1‑251 (MQ=255)                                                                                                                                                                                                                         ccGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGGGCTGATCATCAACCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTCCTTCTGgcg                         >  2:222374/1‑251 (MQ=255)                                                                                                                                                                                                                          cGACATCCTGGCAGGTGACTGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGgcgc                        >  1:307889/1‑251 (MQ=255)                                                                                                                                                                                                                            aCATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCACGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCAGGTGTTGGACTTCGGGATGCGCTGCACCTCCATCTGGCGCAt                      >  2:176767/1‑251 (MQ=255)                                                                                                                                                                                                                               atcgggtgggTGAATGGGGAATCGCGGTTAACCCGCGAGCAAAAAACCAATTCCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGTCTGGGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGAAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCCTAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATc                     <  1:337139/241‑1 (MQ=255)                                                                                                                                                                                                                                   ggCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTAAAGCCGATGCTCGACAACCAAAAGCAGGAGAACGGCGGGCTGTGGCAACCGAATCATTTGCAGACCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGCGCGATGGAGAAGTGCTGATCATCAACCGCACAGTTATTGCCACGCCGGTGATTGACTTTGGGATGCGCGGCACCTACATCTGGCGCATc                     >  2:337139/1‑245 (MQ=255)                                                                                                                                                                                                                                      aGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCGAt            >  1:542275/1‑251 (MQ=255)                                                                                                                                                                                                                                       ggTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCTCCTACATCTGGCGCATCGCTGCCgctg           >  2:388947/1‑248 (MQ=255)                                                                                                                                                                                                                                          gAATGGCGAATCGCTGATGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGCTGATCACCAACCACAAGCCGGAGTACGAAGGGCTGCGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGAGCGACTTTGCCGCAGAACACAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGTATGCGCTAAACCTACATCtcgcacctccctgacgagggcc        >  2:223851/1‑230 (MQ=255)                                                                                                                                                                                                                                             gggCGAATCGCTGTTGACTCGCGAGCAAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATAATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGGGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCGATGGCCAgg     <  1:287788/250‑1 (MQ=255)                                                                                                                                                                                                                                                 gAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGGGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCGATGCCCAGGTTa  >  2:221163/1‑250 (MQ=255)                                                                                                                                                                                                                                                  aaTCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCGATGGCCa       >  1:58618/1‑244 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                 CAACATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATG‑CGCGTCCGCTGACG‑CTGGAAGACG‑ATCGTTTGAGCTG‑CACCGTTCGCGGCTACAACTTCGCGATCACCT‑TCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCGATGGCCAGGTTA  >  NC_000913/3224651‑3225135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑