Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,120,786	G→A	T165I (ACT→ATT)	glcA ←	glycolate/lactate:H(+) symporter GlcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,120,786	0	G	A	100.0%	111.5 / NA	38	T165I (ACT→ATT)	glcA	glycolate/lactate:H(+) symporter GlcA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/17);  total (21/17)

CGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCGCCGC‑GTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAG‑CAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAAC  >  NC_000913/3120539‑3120983                                                                                                                                                                                                                                                        |                                                                                                                                                                                                        cGGACCAATATAGTTAGATGTAAATAACTGAGTGACAGCGAAGCTTCCCCCAGCAAACAGCGCCGCTGGCCAAGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCCGAACGGCACAAGAACCGACAGGAACGGTAACTGACATCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACACGAATCAGCACGCCCAACGCACCACACGCCACCGTCGCAatat                                                                                                                                                                                                      >  2:470773/1‑251 (MQ=255)            aGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATc                                                                                                                                                                                              >  1:58849/1‑248 (MQ=255)                     tAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCCATCAGACACAGcccc                                                                                                                                                                                  >  1:73957/1‑251 (MQ=255)                       aaGAACTGAGTGACAGCGATGCTTACCCCAGCAACCAGCGCCGCTGGCCACGAATCTTTCACCCCTTTCCAGCCGTCAATCATTGCTACCAGCAAGAACGGCACAAGAACCGACAGGAACGGTAACTGAAGTCCCGACATTGCGCCAAAGTGGAACGGATCGATTCCCGTTACCTGCCCGGCGACCAGATTCGGCACGCCCAACAAACCAAACGCCACCGGCGCAAAATTGGCAATAACACACAGccccac                                                                                                                                                                                >  2:319906/1‑249 (MQ=255)                                     aGCGAAGCTTCACCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCAAAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCAACCGGAGCAATATTGGCAATCAGACACAGCCCCGCCGC‑GTAAAACGGtt                                                                                                                                                                 >  2:114089/1‑251 (MQ=255)                                              tCCCCCAGCACCCAGCCCCGCTGGCCACGCCTCTTTCACCCCTTTCCAGCCCTCCATCATTGCTCCCAGCCAGAACGGCACAAGAACCGACAGGAACGGTACCTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGC‑GTATAACGGTTTTAAGcc                                                                                                                                                          <  1:465906/249‑1 (MQ=255)                                               cccccAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGC‑GTATAACGGTTTGAAGccc                                                                                                                                                         >  1:28263/1‑249 (MQ=255)                                                           aGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGACCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGCCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAAACGGCACGCCCAACGCACCAAACGCCACCGGCGCAATTATGGCAATCAGACACACACCCCCCCC‑GACTAACGGGTTGAAGCCCAGGCccaccaccac                                                                                                                                           >  2:277147/1‑247 (MQ=255)                                                                     cccACGTCTCTTTCCCCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGCCGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGC‑GTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTa                                                                                                                                 <  1:442710/250‑1 (MQ=255)                                                                        aCGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGC‑GTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATc                                                                                                                              <  1:367467/251‑1 (MQ=255)                                                                                 tCACCCCTTTCCAGCCTTCCATCATTGCTCCCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGC‑GTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCgg                                                                                                                      <  1:300149/250‑1 (MQ=255)                                                                                       cTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGATACCTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGGCCAGAATAGGCACGCCCAACGAACCAACCGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCTGCA‑AACGGTTTGAAGCCCAGGCCCACCAGCACCGAACCGGAAAAAGCCACCggagccac                                                                                                                >  2:41371/1‑244 (MQ=255)                                                                                             aGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGC‑GTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAagcc                                                                                                          >  1:265659/1‑250 (MQ=255)                                                                                                  cccacaataccccccaccccgaccGGCAAAAGAACCGAAAGGAACGGAAACTGCCGTCCCGCCATTGCGCCAATGTGGAACGGAGCGATTCCCGTTACCTGCCCGGCGACCAGAATCGGCACGCCCAACGCACCAACCGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGC‑GTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAagccagcc                                                                                                      <  2:180406/226‑1 (MQ=255)                                                                                                             ccccacccagAACGACACAAGACCCGACAGAAGCGGTACCTGACGCCCCGCCATTTCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAACCGGCACTCCCAACGCACCAACCGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGC‑GTATAACTGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGTTCCTTCCAG‑Ca                                                                                        <  2:519790/245‑1 (MQ=255)                                                                                                                cagccagAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGC‑GTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAACGCCAGCCGCTCCTTCCAG‑CAACg                                                                                     >  1:217108/1‑251 (MQ=255)                                                                                                                  gccagAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGC‑GTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAG‑CAACGc                                                                                    >  1:474791/1‑250 (MQ=255)                                                                                                                    cagAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGC‑GTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAG‑CAACGCAcc                                                                                 >  1:538128/1‑251 (MQ=255)                                                                                                                       aaCGGCACAAGAACCGACAGGAACGGTAACTGACGTCACGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCACCGCACCAAACGCCACCGGCGCAATATTGGCATTCAGACACAGCCACGCCGC‑GTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCCCCCCGGTAAACCACACCGGCGCCCCAAAGCCAGCCGATCCCTCAAG‑CAACGAACCaa                                                                               >  2:428144/1‑250 (MQ=255)                                                                                                                        aCGGCACAAAACCCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACGGCCCCGCCGC‑GTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAG‑CAACGCACCAAAg                                                                             <  1:319906/251‑1 (MQ=255)                                                                                                                          gccacaAGAACCGTGAGAAACGGTAACTGTCGCCCCGCCATTGCGCCAATGTGGAACGGACCCATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAACCGCCACCGGCGCAATATTGGCAATCAGACACATCCCCGCCGC‑GTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAG‑CAACGCACCAAAGga                                                                           <  1:41371/249‑1 (MQ=255)                                                                                                                                  aaCCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGC‑GTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAG‑CAACGCACCAAAGGAGAAACCaa                                                                   <  1:440176/251‑1 (MQ=255)                                                                                                                                                         gTCCCGCCCTTGCGCCAATGTGGAACAGATCGCTTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCAAACGAAACCCACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGC‑GTATAACGGTTTGAAACCCAGGCCCACCAGCAGCGCACCAGTAATCGCCACCGGCGACCCAACGCCAGCAGCTCATACCGGACAA‑GCACCAAAGGAGCAACGAATCAGCAACACCTGCAAACGCt                                              >  2:348957/1‑249 (MQ=255)                                                                                                                                                         gTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTCCCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGC‑GTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAG‑CAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCt                                              <  1:348957/249‑1 (MQ=255)                                                                                                                                                           cccGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGC‑GTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAG‑CAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGAtc                                          <  1:332251/251‑1 (MQ=255)                                                                                                                                                              gCCATTGCGCCAATGTGGGACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGC‑GTATAACGGATTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAG‑CAACGCACCACAGGAGAAACCAATCAGTAACACCTGCAAACGATGAtcgtc                                       >  1:195203/1‑251 (MQ=255)                                                                                                                                                               ccatggccccactggggAACGGATCGATCCCCGTTCCCTGCCCGGCAACCAGAATCGGCCCGCCCAACGCCCCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGC‑GTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAG‑CAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGAtcgtcg                                      <  2:265659/236‑1 (MQ=255)                                                                                                                                                                cATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGC‑GTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAG‑CAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCgg                                     >  1:203525/1‑251 (MQ=255)                                                                                                                                                                   tGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGC‑GTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAG‑CAAc                                                                                      >  1:534148/1‑199 (MQ=255)                                                                                                                                                                         aaTGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGC‑GTCTAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCAGCACCTTCCAG‑CAACGCACCAAAGGAGAAACCACTCAGCAACACCTGCAAACGCTGATCGTCGGTGATgg                               >  2:422320/1‑248 (MQ=255)                                                                                                                                                                                       cgatccccgtacccTGACCGGCGCCCAGAATGGGCCCGCCCAACCCACCAACCGCCACCGGCGCAATATGGGCAATCAGACACGGCCCCGCCGC‑GTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAG‑CAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCgg              <  2:474791/239‑1 (MQ=255)                                                                                                                                                                                         aTTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGC‑GTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAG‑CAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCAAGCTGCgggt            >  2:96085/1‑249 (MQ=255)                                                                                                                                                                                                ttACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGC‑GTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAG‑CAACGCACCAAAGGAGAAACCAATCAGTAACACCTg                                                      >  2:268678/1‑202 (MQ=255)                                                                                                                                                                                                ttACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGC‑GTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAG‑CAACGCACCAAAGGAGAAACCAATCAGTAACACCTg                                                      <  1:268678/202‑1 (MQ=255)                                                                                                                                                                                                   cccgaccgcactccctaaTCAGCACGACCAACCCCCCAGAGGCCCCCGGCGCAAGATGGGCAATCAGACACAGCCCCGCAGC‑GTATAACGGGTTCAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGAACCAAAGCCAGCCGCTCCTTCCAG‑CAACGCACCAAAGGAGAAACCAATCAGTAACACCAGAAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTTCGGATAATATCGAAc  <  2:173560/235‑1 (MQ=255)                                                                                                                                                                                                   ccTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGC‑GTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAG‑CAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGAtcg                                         >  2:256559/1‑212 (MQ=255)                                                                                                                                                                                                   ccTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGC‑GTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAG‑CAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGAtcg                                         <  1:256559/212‑1 (MQ=255)                                                                                                                                                                                                            cGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGC‑GTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAG‑CAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCt                  >  2:174411/1‑226 (MQ=255)                                                                                                                                                                                                            cGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGC‑GTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAG‑CAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCt                  <  1:174411/226‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                        CGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCGCCGC‑GTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAG‑CAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAAC  >  NC_000913/3120539‑3120983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑