Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,867,454	T→A	Q33L (CAG→CTG)	rpoS ←	RNA polymerase sigma factor RpoS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,867,454	0	T	A	100.0%	146.7 / NA	45	Q33L (CAG→CTG)	rpoS	RNA polymerase sigma factor RpoS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (22/23);  total (22/23)

CGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCG‑ATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGG  >  NC_000913/2867232‑2867693                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                cggGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCG‑ATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAAc                                                                                                                                                                                                                     >  2:115826/1‑251 (MQ=255)           tcaccaccAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTGCTTCGGCCGTTAACAGTGGTGAATAACCAATCGCACCAAGGTAAAGCTGAGTCGCGTCCAACACTCGCTGTGTGGCTCCCTGCG‑ATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAAcgcc                                                                                                                                                                                                            <  2:191350/248‑4 (MQ=255)                      cGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGCCGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCG‑ATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTACGGCCTTTTCGTCAACAACCTCAACTCCGTTCtcatcta                                                                                                                                                                                                 >  2:553211/1‑248 (MQ=255)                                   cGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCG‑ATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCa                                                                                                                                                                                   <  1:547426/251‑1 (MQ=255)                                        ttacggcggCGAGAGGCGACATCTCCAAGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCACCCTGCG‑ATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTATACTAAGACCTTTTCGTCAAAAACCTCAACTACGATATCATCAAATTCAGCATCTTCATTTa                                                                                                                                                                               >  2:441356/4‑250 (MQ=255)                                                                     cAGTGCTCGACACGCAAAATAATCTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTTTGTGGCTCCCTGCT‑ATAACAGTTCCTCTGCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTGCATTTAAATCATGAACTTTCAGCGTATTCTGACTCa                                                                                                                                                 <  2:434372/251‑1 (MQ=255)                                                                         gcgcGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCG‑ATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATaa                                                                                                                                              <  1:140115/250‑1 (MQ=255)                                                                            cgACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAACGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCG‑ATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATACGGTg                                                                                                                                          >  2:145745/1‑251 (MQ=255)                                                                             gACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATTACCAATCGCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCG‑ATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTgg                                                                                                                                         <  2:541959/251‑1 (MQ=255)                                                                                gcgcAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCG‑ATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTc                                                                                                                                      <  1:222359/251‑1 (MQ=255)                                                                                   cAAAATAAACTTCTTCTGCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCG‑ATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTa                                                                                                                                   <  1:71441/251‑1 (MQ=255)                                                                                    aaaaTAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTCAGTCGCGTCCACCACACGCTGTGTGGCTCCCTGCG‑ATAACAGTTACTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTCGCTCCTAc                                                                                                                                  >  1:65352/1‑251 (MQ=255)                                                                                       aTAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCG‑ATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCg                                                                                                                               >  1:326447/1‑251 (MQ=255)                                                                                                ttcttcGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCG‑ATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCtt                                                                                                                      >  1:349733/1‑251 (MQ=255)                                                                                                    tcGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCG‑ATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGAc                                                                                                                   <  1:273053/250‑1 (MQ=255)                                                                                                        ccGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCG‑ATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAAc                                                                                                              >  1:284580/1‑251 (MQ=255)                                                                                                            tAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCG‑ATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTc                                                                                                          >  1:239596/1‑251 (MQ=255)                                                                                                             aaCAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCG‑ATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCa                                                                                                         <  1:167414/251‑1 (MQ=255)                                                                                                               cAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCG‑ATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCa                                                                                                         >  2:152461/1‑249 (MQ=255)                                                                                                                 gtggtgAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCACCCTGCG‑ATAACAGATCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGACCTTTTCGTCAAAAACCTCAACTCCGTTCTCATACAATTCAGCATCTTCATTTTAATCATGAACTTTCAGCGTATTCTGACTCTTAAGGTGGCTCCTACCCGTGATCCCTTGACGGCACATTCAAGc                                                                                                      >  2:137309/1‑250 (MQ=255)                                                                                                                  tggtgAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCG‑ATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCa                                                                                                     >  1:68296/1‑250 (MQ=255)                                                                                                                   ggtgAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCG‑ATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATc                                                                                                                                                                           <  2:42979/179‑1 (MQ=255)                                                                                                                   ggtgAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCG‑ATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATc                                                                                                                                                                           >  1:42979/1‑179 (MQ=255)                                                                                                                                   cACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCG‑ATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAGCCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTccg                                                                                       <  1:311598/247‑1 (MQ=255)                                                                                                                                     ccAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCG‑ATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGAtt                                                                                 >  1:351263/1‑251 (MQ=255)                                                                                                                                             aaGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCG‑ATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATACGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTg                                                                         >  2:3365/1‑251 (MQ=255)                                                                                                                                                         gTCCAACACACGCTGTGTGGCTCCCTGCG‑ATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCAGAGGCAAATAACg                                                             >  2:397756/1‑251 (MQ=255)                                                                                                                                                                acacGCTGTGTGGCTCCCTGCG‑ATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCGTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCggg                                                      <  1:152461/251‑1 (MQ=255)                                                                                                                                                                 cacGCTGTGTGGCTCCCTGCG‑ATAACAGTCCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGt                                                     <  1:553211/251‑1 (MQ=255)                                                                                                                                                                  cctcTGTGTGGTTCCCTGCG‑ATATCAGTTCCTCTTCGTCCAAATCGTTATCACTGGGTTCCATTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATACTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGtt                                                    <  2:148614/248‑1 (MQ=255)                                                                                                                                                                    gctggttgGCTCCC‑GCGGATAACAGCTCCCCTTCGGCCAAATCTTTATCTCTGGGTTCCAGGTCTACTAAGGTCTTTTCGTAAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACGTTCAGCGTAATCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGGCGGAACATACAGGCAAAAGCTTGTTTCCGCCGATTTAGCGCTGCGGCAAATAACGCAGCGGGTTTa                                                  <  2:138485/245‑1 (MQ=255)                                                                                                                                                                       gtgtgGCTCCCTGCG‑ATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTAc                                                 <  1:138585/249‑1 (MQ=255)                                                                                                                                                                          tgGCTCCCTGCG‑ATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGAtt                                            >  1:127978/1‑251 (MQ=255)                                                                                                                                                                                ccTGCG‑ATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCGCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCt                                      <  1:365535/251‑1 (MQ=255)                                                                                                                                                                                   gCG‑ATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCtt                                     <  1:397756/249‑1 (MQ=255)                                                                                                                                                                                       aTAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAAc                                <  1:233476/251‑1 (MQ=255)                                                                                                                                                                                        tAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACg                               >  1:61173/1‑251 (MQ=255)                                                                                                                                                                                                             aTCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGtt                                                                                                                                                                                                         >  2:30174/1‑60 (MQ=255)                                                                                                                                                                                                             aTCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGtt                                                                                                                                                                                                         <  1:30174/60‑1 (MQ=255)                                                                                                                                                                                                                gTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCg                                                                                                                               <  2:110932/131‑1 (MQ=255)                                                                                                                                                                                                                gTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCg                                                                                                                               >  1:110932/1‑131 (MQ=255)                                                                                                                                                                                                                     cACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCCTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTg   <  1:115826/250‑1 (MQ=255)                                                                                                                                                                                                                     cACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATATTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGAGTTGAACTgg  >  1:102141/1‑251 (MQ=255)                                                                                                                                                                                                                            ttCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCgg                                                                      <  1:546832/176‑1 (MQ=255)                                                                                                                                                                                                                            ttCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCgg                                                                      >  2:546832/1‑176 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                CGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCG‑ATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGG  >  NC_000913/2867232‑2867693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑