Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,789,286	G→A	G101D (GGT→GAT)	glaH →	glutarate dioxygenase GlaH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,789,286	0	G	A	98.0%	140.1 / NA	49	G101D (GGT→GAT)	glaH	glutarate dioxygenase GlaH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (24/24);  minor base C (1/0);  total (25/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGATTCACCCTCACCCCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGGTGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTG‑AAAAATGTCGATAACTCAGA‑CAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACGTATGTCGAAGAGATAACCGATTACGTGCTGATGATGAAAATCGACGAGCAAAACA  >  NC_000913/2789041‑2789531                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                        cGGATTCACCCTCACCCCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCg                                                                                                                                                                                                                                                    >  1:29908/1‑251 (MQ=255)    aTTCACCCTCTCCCCGACTGCGCAATCCCCGCGTCTGCTGGAACTCTCCTTCACTGAACCGCCGCCCAAACATTTTCTTGAGCAGGTTTCCGAGTGGCCTGTGCATGCGCTTGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATGTATGTGCGAACCAGCTGCTGCCATTTCTGGTGAAGACGTTGCTAGACCGCGCTGAAGGTGCGTTGTTGATCAATGCGTTGGATGTCgatg                                                                                                                                                                                                                                                 <  2:345228/251‑1 (MQ=255)         ccctcacccCTGCGGCGCAATCCCCGCGGCTGCTGGAACTCACCTTCACCGAACAGACGACCAAATAGTTTCTTGGGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTATAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGAtgtg                                                                                                                                                                                                                                            <  2:179102/251‑1 (MQ=255)             cacccCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGaagc                                                                                                                                                                                                                                        <  2:175305/251‑1 (MQ=255)                   gTCGGCGCACTCCCCGCGACTGCTGGAACTCACCTTCACCGAACAGCCGAACAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGCGCAGGCGCTGGAGTACACATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAACTGCAACCATTGCTGTTGAAGGCGTTGCTAAACCGCGCTGATGGTGCGTTGTTGCTCCATGCGGTGGCTGTCGATGCTGTGGAGCAAGCgg                                                                                                                                                                                                                                  >  2:199509/1‑251 (MQ=255)                   gTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCgg                                                                                                                                                                                                                                  >  1:179330/1‑251 (MQ=255)                         gcAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATg                                                                                                                                                                                                                               >  1:522796/1‑248 (MQ=255)                              ccccGTGTCTGCTTGAACTCACCTTCACCGAACAGACGACCAAACAGTATCTTGAGCAGGTTTCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTTCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGGGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGt                                                                                                                                                                                                                        <  2:341462/250‑1 (MQ=255)                                 cgcgTCTGCTGGAACTCACCTTCACCGAACAGTCGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGGTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAg                                                                                                                                                                                                                    <  2:169679/251‑1 (MQ=255)                                    gTCTTCTGGAACACACCTTCCCCGAACGGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGGGGCCCGTGCAGGCGCTGGAGTACAAAGCGGTTCTGCGTTGTCGGGTAGCCAAAATTCTGGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCTGTGGATGTCGATGATGTGAAGCATGCGGATGAGATGGTGAAGCTg                                                                                                                                                                                                                 <  2:395672/251‑1 (MQ=255)                                        gctgGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCgg                                                                                                                                                                                                             >  1:496768/1‑250 (MQ=255)                                         ctgGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGCACCAGCTGCAACCATTGCTGTTGCAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCAGTGGATGTCGATGGTGTGAAGCAAGCGGGTGAGATGGTGAAGCTGGCg                                                                                                                                                                                                              >  2:15416/1‑249 (MQ=255)                                                  aCCTTCCCCGAACAGACGACCAAACAGTTTCTTGAGCAGGCTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGgc                                                                                                                                                                                                   <  2:216949/251‑1 (MQ=255)                                                               aGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCATGCGCTGGAGTACAACTCGTTTATGCGTTTTCGGGTAGCCAAAATCCTTGACGATCTATGTGCGAACCAGCTGAAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATTTCGTTGATGGGAAGCAAGCGGCTGAGATGGGGAAGCTGGCTACGGCGGTGGCGCATCTGCTTggg                                                                                                                                                                                      >  2:330535/1‑250 (MQ=255)                                                                            gtttcgtgagatggTTGCCGAGTGTACCGTGCAGGCGCTGGAGTACAAAGCGTGTCTGCGTTTGCGTGTAGCTAAAATTCTTGACGAGCTATTGGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTTAAGCTGGCGACGGCGGTGGCGCAGCTGATTGGCCGTTCCAATTTc                                                                                                                                                                          <  2:24353/238‑1 (MQ=255)                                                                                  tGAGCAGGTTGCCGAGTGGCCCGTGCAGGCTCTTGAGTACAAATCGTGTCGGCGTTTGCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCATCCATGTCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGACGCTGGCGACGGCGGTGGCGCATCTGATGGGCCGTTGCAATTTcgatgc                                                                                                                                                                     <  2:10908/249‑1 (MQ=255)                                                                                      cAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACCGCGGTGGGGCATCTGATTGGCCGGTCCAATTTcgatgcgatggg                                                                                                                                                               >  1:319971/1‑249 (MQ=255)                                                                                       aGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGc                                                                                                                                                              <  1:399896/251‑1 (MQ=255)                                                                                            gCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTGTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCa                                                                                                                                                         <  2:496768/251‑1 (MQ=255)                                                                                                   ggCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGGGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTAc                                                                                                                                                  <  1:3948/251‑1 (MQ=255)                                                                                                     cccGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGAGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTAcgc                                                                                                                                                >  1:66943/1‑251 (MQ=255)                                                                                                      ccGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGAAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGGGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGCGATGGTGAAGCTGGCGACGGCGGGGGGGCCTCTGATGGGGCGTTACAATTTcgatgcagtgtgaggtcaagatatcgcc                                                                                                                                               >  2:413557/1‑229 (MQ=255)                                                                                                                   ggAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTG‑a                                                                                                                                 <  2:179330/251‑1 (MQ=255)                                                                                                                   ggAGTACAAAGCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTGGTAGt                                                                                                                                    <  1:306623/249‑1 (MQ=255)                                                                                                                          aaaTCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTG‑AAAAATGt                                                                                                                          >  1:31190/1‑251 (MQ=255)                                                                                                                           aaTCGTTTCTGCGTTTTCGGGTCGCCAAAATTCTTGACGCTCTATGTGCGAACCCGCTGCAACCATTGCTGTTGAAGACGTTGCTACACCGCGCTGCAGGTGCGTTGTTGATCAATGCGGCGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGACGCTGGCGACGGCGGCGGCGCATCTGATTGGCCGTTCCCATTTCCATGCGTACAGCGGTCAGCATTACGCGCGTTTTGTCGTG‑AAAAAtctc                                                                                                                         >  2:52473/1‑248 (MQ=255)                                                                                                                                         tttCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGATGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGGGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGAGGCGACTCTGATTGGCCGTTCCGATTTTGATGCGATGCGCGGTCCGGATTACGCGCGTTTTGTAGTG‑ATAAATGGCAATAACTCAggc                                                                                                             >  2:367290/1‑247 (MQ=255)                                                                                                                                                    cAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTa                                                                                                                                      <  1:43645/214‑1 (MQ=255)                                                                                                                                                    cAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGGGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGGCAGTATTACGCGCGTTTTGTa                                                                                                                                      >  2:43645/1‑214 (MQ=255)                                                                                                                                                              gACGATCTATGTGCGAACCAGCTGCAACCCTTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGATGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGAAAGCGGATGAGATGGTGAAGCTGGCGACGGCGCGGGAGCATCTGATTGGCCGATCCAATTTCGAAGCGGAGAGCGGTCCGTATTACGCGCGGTTTGTAGGGCAAAAATGT‑GATAACTCAGG‑AAGCTAACTGCGCCAGCCGCAcc                                                                                      >  2:547722/1‑250 (MQ=255)                                                                                                                                                                cGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCCATTTCGATGCGATGAGCGGTCTGTGTTACGCGCGTTTTGTAGTG‑CAAAATGTCGATAAACCAGA‑GCGCTCTCTGCGCCAGCCGCACcgcg                                                                                   >  2:14038/1‑251 (MQ=255)                                                                                                                                                                cGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGa                                                                                                                                                                >  1:81694/1‑176 (MQ=255)                                                                                                                                                                cGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGACGCGATGa                                                                                                                                                                <  2:81694/176‑1 (MQ=255)                                                                                                                                                                            gAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCCATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTTGTG‑AAAAATGTCGATAACTCAGA‑CAGCTATCTGCGCCAGCCGccacgcggggtgggacatc                                                                       >  2:265985/1‑233 (MQ=255)                                                                                                                                                                               ccAGCTGCAACCATTGCTGTTGAAGAAGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGGCAGTATTACGCGCGTTTTGTAGTG‑AAAAATGTCGATAACTCAGA‑CAGCTATCTGCGCCAGCCGCACCGCGGGATGGAGCTacac                                                                     >  1:141674/1‑250 (MQ=255)                                                                                                                                                                                          cATTGCTGTTGAAGACGTTGCTAAACCGCGATGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGGG‑TAAAATGTCGATAACTCAGAACA‑CTATCTGCGCCAGCCGCACAGCGGGATGGAGCTACACAacgacgacccg                                                         >  2:374297/1‑246 (MQ=255)                                                                                                                                                                                            tggttgtggAAGTCGTTGCTAAACCGCGCTGAAGGTGCGGTGTGGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTG‑AAAAATGTCGATAACTCAGA‑CAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACg                                                         <  2:319971/241‑1 (MQ=255)                                                                                                                                                                                                 gTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGCGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTG‑AAAAATGTCGATAACTCAGA‑CAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACGTATGTc                                                   <  1:456810/250‑1 (MQ=255)                                                                                                                                                                                                    gAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTG‑AAAAATGTCGATAACTCAGA‑CAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACGTATGTCGAag                                               <  1:15416/251‑1 (MQ=255)                                                                                                                                                                                                             gCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTG‑AAAAATGTCGATAACTCAGA‑CAGCTATCTGCGcc                                                                                               <  1:334611/194‑1 (MQ=255)                                                                                                                                                                                                               aaaaCCGCGTGGAAGGTGCGTTGTTGATAAATGCGGTGTATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTGCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTG‑AAAAATGTCGATAACTCAGT‑CAGCTATCTGCGCCAGCCGCAACGCGTGATGGAGCTACACAACGACGGCACGTATGTCGAAGAGATATCCGAt                                    <  2:518182/250‑1 (MQ=255)                                                                                                                                                                                                                 aaCCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTG‑AAAAATGTCGATAACTCAGA‑CAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACGTATGTCGAAGAGATAACCGATTa                                  <  2:522796/251‑1 (MQ=255)                                                                                                                                                                                                                     gcgcTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTG‑AAAAATGTCGATAACTCAGA‑CAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACGTATGTCGAAGAGATAACCGATTACGTg                              <  1:34754/251‑1 (MQ=255)                                                                                                                                                                                                                            aGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTG‑AAAAATGTCGATAACTCAGA‑CAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACGTATGTCGAAGAGATAACCGATTACGTGCtgatga                       <  1:199509/251‑1 (MQ=255)                                                                                                                                                                                                                              gTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTG‑AAAAATGTCGATAACTCAGA‑CAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACGTATGTCGAAGAGATAACCGATTACGTGCtgatggtg                     >  1:294764/1‑248 (MQ=255)                                                                                                                                                                                                                                         aTCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGGG‑AAAAATGTCGATAACTCAGA‑CAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACGTATGTCGAAGAGATAACCGATTACGTGCTGATGTTGAAAATcgacg           >  1:565668/1‑250 (MQ=255)                                                                                                                                                                                                                                         aTCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATATGATTGGCCGTTTCAATTTCGATGCGATGAGCGGTCAGTCTTACCCGCGTTTTGTAGTG‑AAAAATGTCGATAACTCAGA‑CAGCTATCTGCGCCCGCCGGACCGCGTGGTGGAGCTTCCCAACGACGGCCCGTATGGCATAACGTTAACCGATTACGTGccgtatatgaaaatagacc           >  2:437482/1‑232 (MQ=255)                                                                                                                                                                                                                                           cAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTG‑AAAAATGTCGATAACTCAGA‑CAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACGTATGTCGAAGAGATAACCGATTACGTGCTGATGATGAAAATCGACGAGc        >  1:44426/1‑251 (MQ=255)                                                                                                                                                                                                                                            aaTGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTG‑AAAAATGTCGATAACTCAGA‑CAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACGTATGTCGAAGAGATAACCGATTACGTGCTGATGATGAAAATCGACGAGc        >  1:105199/1‑250 (MQ=255)                                                                                                                                                                                                                                                 ggtggatgTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTG‑AAAAATGTCGATAACTCAGA‑CAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACGTATGTCGAAGAGATAACCGATTACGTGCTGATGATGAAAATCGACGAGCAAAACa  <  1:209714/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                        CGGATTCACCCTCACCCCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGGTGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTG‑AAAAATGTCGATAACTCAGA‑CAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACGTATGTCGAAGAGATAACCGATTACGTGCTGATGATGAAAATCGACGAGCAAAACA  >  NC_000913/2789041‑2789531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑