Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	502,232	G→A	T336I (ACC→ATC)	ybaL ←	putative transporter YbaL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	502,232	0	G	A	100.0%	139.4 / NA	44	T336I (ACC→ATC)	ybaL	putative transporter YbaL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/21);  total (23/21)

ATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCA‑GTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTC‑AATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAAC‑GGATC‑AAACAACATCCCGACGGAGACAA‑AAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACG  >  NC_000913/501992‑502478                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                          atatCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCA‑GTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTC‑AATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGt                                                                                                                                                                                                                                                 >  1:393124/1‑251 (MQ=255)       aCTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCA‑GTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTC‑AATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTg                                                                                                                                                                                                                                           >  2:121789/1‑251 (MQ=255)           ggATCTGCTTCTCTGCGTCGATTGCTGTTTCCAGCGTCTGCTCTTCCACCGTTTCGGTTTTCGCGAGAAATTTCTCAATGT‑GGGCAAACATTACCGGTTGGAGCTAAATCTACGGGATCGCCCCTGCCGGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTC‑AATGCCATGCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATTTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAg                                                                                                                                                                                                                                       <  1:215418/251‑1 (MQ=255)                      tcttctTCGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCA‑GTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTC‑AATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAAc                                                                                                                                                                                                                              >  1:262859/1‑249 (MQ=255)                           ttcGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCA‑GTAGTGCGCACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTC‑AATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGcc                                                                                                                                                                                                                       >  2:92061/1‑250 (MQ=255)                             cGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCA‑GTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTC‑AATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCAcc                                                                                                                                                                                                                     >  1:175845/1‑251 (MQ=255)                                    ctctTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCA‑GTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTC‑AATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACAAATCTGCCCCAGGCTGGCGGCGCTGATTAATGCCGGACGTTGGGAGTGCCCAAACAGACGCCCCAGGaaa                                                                                                                                                                                                               >  2:566286/1‑250 (MQ=255)                                                 ctctctTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCA‑GTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGAGCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAACTTC‑AATGCCATTCCCAGTCCCGCCAGGATAACCGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGATGGGAGTAACCAAACAGTCGCACCAGGAAAAATGCGGCTAACg                                                                                                                                                                                                  >  1:307975/3‑250 (MQ=255)                                                       cccAGCGTTTCGGTCTTCGCCAGATATTTCTCCA‑GTAGTGCGAACAGTACCGGGTGGAGCATAACCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTC‑AATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTAcc                                                                                                                                                                                           <  1:475705/250‑1 (MQ=255)                                                        ccAGCTTTTCGGTCTTCCCCAGATATTTCTCCA‑GTAGTGCGGACAGTACCGGGTTGAGCATAATCGACAGGACCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTC‑AATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTAcc                                                                                                                                                                                           <  1:445185/250‑1 (MQ=255)                                                                 cGGTCTTCGCCAGATATTTCTCCA‑GTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTC‑AATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGAACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGaaa                                                                                                                                                                                  >  2:97655/1‑249 (MQ=255)                                                                          ccAGATATTTCTCCA‑GTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTC‑AATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGc                                                                                                                                                                        >  1:140263/1‑251 (MQ=255)                                                                                   tctcCA‑GTAGGGCGACCAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTC‑AATGCCATCCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAg                                                                                                                                                                <  1:381728/250‑1 (MQ=255)                                                                                                 gAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTC‑AATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGtt                                                                                                                                                  >  1:81155/1‑251 (MQ=255)                                                                                                        cccGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTC‑AATGCCATCCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGaa                                                                                                                                            <  1:548986/249‑1 (MQ=255)                                                                                                            ggtagcgcaaaaacacgaGGACCGCCCCTGCCAGTACCAGGTTTTGCCCGGCCTGCGGCAGTAAATTC‑AAGGCCAGTCCCAGGCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGGCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGa                                                                                                                                       <  2:31671/234‑1 (MQ=255)                                                                                                               tGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTC‑AATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTACGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAACCAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGATGCTGAATCAGAAtt                                                                                                                                    >  2:292640/1‑251 (MQ=255)                                                                                                                  gCATAACCGACAGGACCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTC‑AATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAAc                                                                                                                                 <  1:199100/251‑1 (MQ=255)                                                                                                                         cGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTC‑AATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGa                                                                                                                                                                                    >  2:134021/1‑193 (MQ=255)                                                                                                                         cGACAGGATCGCCCCGGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTC‑AATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCATGAAAAATGCGGCTAACGACTTACCAAACAGa                                                                                                                                                                                    <  1:134021/193‑1 (MQ=255)                                                                                                                               gtgcaccccTGCCAGTCCCATGTGTTGCCCGGCCTGCGGCAATAAATGC‑AATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACCCACCAATCTGCGCCAGGCTGTCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACATTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCTTCTCCAGCACTGCCAGCGGTTGTTGAATCAGAATTAAC‑GGATC‑AAacaaca                                                                                                                  <  2:322578/246‑1 (MQ=255)                                                                                                                                 tCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTC‑AATGCCATTCCCAGTCCCGCCAGGCTAAACGAGAACTCACCCATCTGCGCCAGGCTGGCGGCGATGATTAATGACGTACGTTGGGCGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACGGAATAATCGCCAGCGCCGCCAGCACAGCCAGCGGTTCCTGGATAAGAATTAAC‑GGCTC‑AAacaacaac                                                                                                                >  2:218325/1‑249 (MQ=255)                                                                                                                                  ccccccggccattccctggTTTTGCCCGGCCGGCGTCAG‑AAATTCCAATGCCATTCCCAGCCCCGCCAGGATAAACGCGAACTCACCAATCTTCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTCCCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAAC‑GGATC‑AAACAACATcc                                                                                                               <  2:71996/234‑1 (MQ=255)                                                                                                                                    ccccTGCCATTACCAGGTTTTGCCCGGCCTGCGGCAGTAAATTC‑AATGCCATTCCCAGTCCCTCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAAC‑GGATC‑AAACAACATCCCg                                                                                                             <  1:281346/251‑1 (MQ=255)                                                                                                                                       cTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTC‑AATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAAC‑GGATC‑AAACAACATCCCGACg                                                                                                          >  1:207720/1‑251 (MQ=255)                                                                                                                                         gCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTC‑AATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAAC‑GGATC‑AAACAACATCCCGACg                                                                                                          <  1:174178/249‑1 (MQ=255)                                                                                                                                             gcaCCAGGTTTTGTCCGGCCTGCGGAAGTAAATTC‑AATGCAATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAAC‑GGATC‑AAACAACATCCCGACGGAGACa                                                                                                    <  2:125869/249‑1 (MQ=255)                                                                                                                                                      tttGTCCGGCCTGCGGCAGTAAATTC‑AATGCCATTCCCAGTCCCGCCAGGATACACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCAAGCACTGCCAGCGGTTGCTGAATCAGAATTAAC‑GGAGC‑AAACAACATCCCGACGGAGACAA‑AAAACAGc                                                                                          >  1:334251/1‑251 (MQ=255)                                                                                                                                                             gtccTGCGGCAGTAAATTC‑AATGCCATTCCCAGTCCCGACAGGATAAACGCGAACTCACCAATATGCGCCAAGCTAGCGGCGATGATACATGCCGTACGTTGGGAGTGACCAAAAAGACGCACCAGGAAAACTGCGGCTAACGAATTCCCACAAAGACTAAACGACAGCGTCGCCAGCCCTGCCAGCGGTTGCTGAATCAGAAT‑AACAGGATC‑AAACAACAACCCGACGGAGCCAC‑ACAACAGCAccaca                                                                                    >  2:569617/3‑247 (MQ=255)                                                                                                                                                             aacctacGGCAATAAATTC‑TATGGCATTCCCAGACCCGCCTGGATAACCGCGCACTCACCAATCTGCTCCAGGCTGGCGACGATGATTAATGCCGTACGTTGAGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTACCGACTGAACAAACGGACTACACTCCCACTTCACAAGCACGGCAAGCGGTTGCTGAATCAGTATTAAC‑AGATCCGAACAACA‑CCCGACGGAGAAAACAAAACAACACCga                                                                                     >  2:569597/7‑249 (MQ=255)                                                                                                                                                                               c‑AATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAAC‑TGATC‑AAACAACATCCCGACGGAGACAA‑AAAACAGCACCGCAAACGCGTCGCGCAATGGCa                                                                 <  1:230839/251‑1 (MQ=255)                                                                                                                                                                                 aaTGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTTAATCAGAATTAAC‑GGATC‑AAACAACATCCCGAc                                                                                                           <  1:478517/209‑1 (MQ=255)                                                                                                                                                                                               tCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAAC‑GGATC‑AAACAACATCCCGACGGAGACAA‑AAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGggcgg                                                  >  1:53289/1‑251 (MQ=255)                                                                                                                                                                                                 ccGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAAC‑GGATC‑AAACAACATCCCGACGGAGACAA‑AAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCa                                                <  1:92061/251‑1 (MQ=255)                                                                                                                                                                                                  cGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAAC‑GGATC‑AAACAACATCCCGACGGAGACAA‑AAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCAc                                               <  1:121789/251‑1 (MQ=255)                                                                                                                                                                                                     cAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAAC‑GGATC‑AAACAACATCCCGACGGAGACAA‑AAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACggg                                            >  1:64054/1‑250 (MQ=255)                                                                                                                                                                                                           aaaCGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAAC‑GGATC‑AAACAACATCCCGACGGAGACAA‑AAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTc                                       >  1:229977/1‑250 (MQ=255)                                                                                                                                                                                                                        ccAATCGGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAAC‑GGATC‑AAACAACATCCCGACGGAGACAA‑AAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGTGGCACGGTGACTCAGTTCAGACTCGtt                         <  1:509197/251‑1 (MQ=255)                                                                                                                                                                                                                                 gcCAGGCTGGCGGTGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAAC‑GGATC‑AAACAACATCCCGACGGAGACAA‑AAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCAt                <  2:229977/251‑1 (MQ=255)                                                                                                                                                                                                                                 gcCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAAC‑GGATC‑AAACAACATCCCGACGGAGACAA‑AAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCAt                <  1:170748/251‑1 (MQ=255)                                                                                                                                                                                                                                   cAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAAC‑GGATC‑AAACAACATCCCGACGGAGACAA‑AAAACAGCACCGCAACCGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATcc              >  1:525459/1‑251 (MQ=255)                                                                                                                                                                                                                                    aGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAAC‑GGATC‑AAACAACATCCCGACGGAGACAA‑AAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATccc             >  1:109718/1‑251 (MQ=255)                                                                                                                                                                                                                                    aGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAAC‑GGATC‑AAACAACATCCCGACGGAGACAA‑AAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATccc             >  1:475061/1‑251 (MQ=255)                                                                                                                                                                                                                                           cggcgATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAAC‑GGATC‑AAACAACATCCCGACGGAGACAA‑AAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAg      <  1:566286/251‑1 (MQ=255)                                                                                                                                                                                                                                               gatgatTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAAC‑GGATC‑AAACAACATCCCGACGGAGACAA‑AAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACg  >  1:186457/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                          ATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCA‑GTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTC‑AATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAAC‑GGATC‑AAACAACATCCCGACGGAGACAA‑AAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACG  >  NC_000913/501992‑502478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑