Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,732,992	G→A	I394I (ATC→ATT)  I394I (ATC→ATT)	clpB ← clpB ←	chaperone protein ClpB chaperone protein ClpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,732,992	0	G	A	100.0%	191.2 / NA	61	I394I (ATC→ATT)  I394I (ATC→ATT)	clpB clpB	chaperone protein ClpB chaperone protein ClpB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/26);  total (35/26)

CGTACCAGAAAGCGATGCCTTCTCTGCTTTCCACTCTTCTTCTAACTCGGAGTACTGACGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCGATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCGTCAAGCGTCG  >  NC_000913/2732758‑2733233                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                  cGTACCAGAAAGCGATGCCTTCTCTGCTTTCCACTCTTCTTCTAACTCGGAGTACTGACGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGCGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATggatt                                                                                                                                                                                                                                   >  2:222184/1‑248 (MQ=255)     ccAGAAAGCGATGCCTTCTCTGCTTTCCACTCTTCTTCTAACTCGGAGTACTGACGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGCCTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGGGTTCTTCTGGTTTTGAGTCAATATGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATc                                                                                                                                                                                                                               >  2:559338/1‑251 (MQ=255)      cAGAAAGCGATGCCTTCTCTGCTTTCCACTCTTCTTCTAACTCGGAGTACTGACGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCTAGTTCTTCTGGTTTTGAGTCACTCTGCATACGAATGCTGGATGCTGCTTCATCACTCAGGTCGATGGCTTTATcc                                                                                                                                                                                                                              >  2:90331/1‑251 (MQ=255)          aaGCGATGCCTTCTCTGCGTTCCACTCATCTTCTAACTCGGAGTACTGACGTTCTTTTTCGCTCAGTTCTGCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGGTGTTCCAGTTTGAGCTGGATGATACGACGATCTAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGGCGATGGCTTTATCCGGCa                                                                                                                                                                                                                          <  2:59077/251‑1 (MQ=255)          aaGCGATGCCTTATCTGCTTTCCACTCTTCCTCTAACTCGGTGTACTGACGTTCTTTGTCGATCAGTTCTTCGTTGCGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGCTCGAGTAGGTCGAGTTCTTCTGGTTTTGAGACAATCTGCATGAGCATGCTGAATTCTGCTTCATCAATCAGGTCGATGGCTTTATCCgca                                                                                                                                                                                                                           >  2:469948/1‑248 (MQ=255)             cGATGCCTTCTCTGCTTTCCACTCTTCTTCTAGCTCGGAGTACTGACGTTCTTTGTCGCTCAGTTCTTCGGTGAGCATATCCATACGTTTTTTACTGGCTTCATCAGGCTCTTTCATTAACGCCTGTTGTGCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTGCTGGTTTTGAGTCAATCTGCATACGAACGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTCTCCGGCAGCt                                                                                                                                                                                                                       <  2:192399/251‑1 (MQ=255)              gATGCCTTCTCTGCTTTCCACTCTTCTTCTAACTCGGAGTACTGACGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTg                                                                                                                                                                                                                      >  1:442147/1‑251 (MQ=255)                        ctGCTTTCCACTCTTCTTCTAACTCGGAGTACTGACGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCa                                                                                                                                                                                                               <  1:246775/248‑1 (MQ=255)                         tgcgttCCACTCTTCTTCTAACTCGGAGTACTGACGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGATTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGCGCTGGATGATACGCCGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACTAAAGCTGGATGCTGCTTCATCATTCAGGTCGATGGCTTTATCCGGCAGCTGACGGCCAGCa                                                                                                                                                                                                            >  2:519934/5‑250 (MQ=255)                                        ttctAACTCGGAGTACTGACGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTTAGTCCATCTGCCTACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGGCGGTCAGCAATGTAGCGATg                                                                                                                                                                                                 >  2:132156/1‑246 (MQ=255)                                        ttctAACTCGGAGTACTGACGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATg                                                                                                                                                                                                 <  1:132156/246‑1 (MQ=255)                                              cTCGGAGTACTGACGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGCATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCGGACGGTCAGCAATGTAGCGATGAGACAACGTcc                                                                                                                                                                                      >  2:25370/1‑250 (MQ=255)                                              cTCGGAGTACTGACGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTcg                                                                                                                                                                                      >  1:566567/1‑251 (MQ=255)                                                    gTACTGACGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTg                                                                                                                                                                                >  1:506077/1‑251 (MQ=255)                                                      aCTGACGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATGTCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGc                                                                                                                                                                               >  1:563825/1‑250 (MQ=255)                                                           cGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAAt                                                                                                                                                                         >  1:508221/1‑251 (MQ=255)                                                                        tCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGCGCTGGATGATACGACGAACGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGGTGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAga                                                                                                                                                             >  1:220379/1‑249 (MQ=255)                                                                              cttcGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGCATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACGACGTCGCCGCTGCAACACTTGCCGGGTCAGTAATTTGc                                                                                                                                                      >  1:16090/1‑251 (MQ=255)                                                                                  gTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTGGCGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGCTGAGCAAACGTCGCCGCTGCAACCATTGCCGGGTCAGTAATTTGCACAt                                                                                                                                                  >  1:366073/1‑251 (MQ=255)                                                                                       ccatttccattctttttttACTGGCTTCATCTGACTCTTTCATTAACGCCTGTTGTTCCTGTTTGAGCTTGATGATACGTCGCGCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACAtggtgg                                                                                                                                             <  2:431186/238‑1 (MQ=255)                                                                                         atatCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGgtggtg                                                                                                                                           >  1:178837/1‑251 (MQ=255)                                                                                          tatCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGc                                                                                                                                          >  1:74759/1‑251 (MQ=255)                                                                                                        tttttGGCTTCATCAGACTCTTTCATTAACGCCTGTTGGTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGtt                                                                                                                             <  2:567618/246‑1 (MQ=255)                                                                                                           cTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCCGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATg                                                                                                                                                                                                         >  2:318061/1‑171 (MQ=255)                                                                                                           cTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTACATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATg                                                                                                                                                                                                         <  1:318061/171‑1 (MQ=255)                                                                                                                               cATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCa                                                                                                      >  1:130557/1‑250 (MQ=255)                                                                                                                                agtAACGCCTGATGTTCCAGTTTGAGCGGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTGTTGAGTCAATCTGCATACGAATGCTTGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAAt                                                                                                    <  2:486839/249‑1 (MQ=255)                                                                                                                                       ccTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGCGGCGGTCGAGATCTTCTGGTTTTGAGTCAATCTGCATACGCATGCTGGATGCTGCTTCATCAATCAGGTCGATGCCTTTATCCGGCAGCTGACGGTCTGCAATGTAGCGATGAGCCAACGGCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACTTGGTGAGGCAATTCGTACCGTTTTTTCAGGCCAAGCAGAATCGCAATGGAATCt                                                                                             >  2:133714/1‑251 (MQ=255)                                                                                                                                          gttgttCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTc                                                                                           <  1:90331/250‑1 (MQ=255)                                                                                                                                          gttgttCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCa                                                                                          <  1:546785/251‑1 (MQ=255)                                                                                                                                             gttCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCCGAATCGCAATGGTATCTTCAAc                                                                                        >  1:349043/1‑250 (MQ=255)                                                                                                                                                 cAGTTTGAGCTTGATGATCAGACGATCGATGCGGTCTAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGTTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTTCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACTCAGAATCGCAATGGTATCTTCAACAGatg                                                                                   <  2:142138/251‑3 (MQ=255)                                                                                                                                                      ttagcgggaggatACGACGATCGAGTCGGTCGAGGTCTTCTGGTTTTGAGGCAAACTTCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTc                                                                               <  2:563825/240‑1 (MQ=255)                                                                                                                                                       gAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCgg                                                                             >  1:553715/1‑251 (MQ=255)                                                                                                                                                             gatgatACGACGCTCGAGGCGGTCGAGGTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAACCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGcaacaa                                                                       <  2:306093/251‑1 (MQ=255)                                                                                                                                                                   acgacgATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACtt                                                                 >  1:373485/1‑251 (MQ=255)                                                                                                                                                                      acgaTCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGATGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGGGCAATTCGTAACGTTATTTCAGGCCAAGCAGAATCGCAATGGAATCTTCAACAGAAGGCTCGGCAACAAACACtttat                                                              >  2:507154/1‑249 (MQ=255)                                                                                                                                                                      aagaTCGAGTCGGTCGAGTTCTTCTGTTTTTGAGTCAATCCGCATACGAATGCTGGATGCCGCTGCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACATCGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCAACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCt                                                              <  2:157278/249‑1 (MQ=255)                                                                                                                                                                         aTCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTgg                                                            >  1:570370/1‑250 (MQ=255)                                                                                                                                                                         aTCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGa                                                           <  1:222184/251‑1 (MQ=255)                                                                                                                                                                           cGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGaaa                                                         <  1:324787/251‑1 (MQ=255)                                                                                                                                                                                cggtcgAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAacgacg                                                    <  1:559338/251‑1 (MQ=255)                                                                                                                                                                                    cgAGTTCTTCTGGTTTTTAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTc                                                 <  2:56644/250‑1 (MQ=255)                                                                                                                                                                                           ttctGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAgcgc                                           >  1:386426/1‑249 (MQ=255)                                                                                                                                                                                            tctGGATTTGAGTCAATCTGCATACGAATGCTGGTTGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCCCCGCTGCAACAATTGCAGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGATTCGCAATGGTAACTTCAACAGAACGCATGGCAACAAACACTTTCTGTAAAAGACGTTCCAGCgcaac                                        >  2:465807/1‑249 (MQ=255)                                                                                                                                                                                                  tttGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTGCTGGAAACGACGTTCCAGCGCAGCATCtt                                   <  1:151825/250‑1 (MQ=255)                                                                                                                                                                                                    tGAGTCAATCTGCATACGAATGCTGGATGCTGCTGCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTGTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCtttt                                 <  2:130557/250‑1 (MQ=255)                                                                                                                                                                                                         cAATCTGCATACGAATGCTGTAAGCTGCTTCATCAATCATGTCTATGGCTTTATCCGGCAGCTGACGGGCAGCAATGGAGCGATGATACAACGTCGCCGCTGCAACAAGTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCATCATCTTTTTCAAt                            <  2:361692/250‑1 (MQ=255)                                                                                                                                                                                                              tGCATACGAATGCTGGATGCTGCTTCCTCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAGTGGTATCTTCAACAGAAGCCTCGGCAACAAACAATTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTg                      >  2:54272/1‑251 (MQ=255)                                                                                                                                                                                                                 aTACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGGGAGTAATTTGCACACGGTGGTGCAAtgc                                                                                                                                     <  1:398825/137‑3 (MQ=255)                                                                                                                                                                                                                 aTACGAATGCTGGATGATGCTTCAGCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGATGCAACAATTGACGGGTCAGTAATTTGCACATGGTGGTGCACTTc                                                                                                                                     >  2:398825/1‑137 (MQ=255)                                                                                                                                                                                                                  tACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCg                   >  1:561709/1‑250 (MQ=255)                                                                                                                                                                                                                      atcgcgggATGCTGCTTCATCAATCAGGTCGATTGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATAtt              <  2:239031/245‑1 (MQ=255)                                                                                                                                                                                                                       atgatGGATGCTGCTTCATCAATCATGTCTATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTTCCTTCAACAGAAGGCTCGGCAACAAACACTTTCTTGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTc             <  2:442147/247‑1 (MQ=255)                                                                                                                                                                                                                          cTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATAtt              >  1:239031/1‑247 (MQ=255)                                                                                                                                                                                                                          cTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGCCGCCGCTGCAACAATTGCCGGGTCAGTAACTTGCACATGGTGGAGAAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACACACACTTTATGGAAACGACGTTCCAGCGACGCATCTTTTTCAATGTACTGGCGATATTCGTc          >  1:539492/1‑251 (MQ=255)                                                                                                                                                                                                                              aTGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACTCTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCGCCAAGc      <  1:124432/251‑1 (MQ=255)                                                                                                                                                                                                                                 ctgctTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCa                              >  1:20348/1‑224 (MQ=255)                                                                                                                                                                                                                                 ctgctTAATCAATCAGGACTATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGGTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCCACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCa                              <  2:20348/224‑1 (MQ=255)                                                                                                                                                                                                                                  tgctTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCGTCAAGcgtcg  >  1:222851/1‑251 (MQ=255)                                                                                                                                                                                                                                  tgctTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAAAGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGCCTCGGCAACAACCACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCGTCCAGcgtcg  >  2:227448/1‑251 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                  CGTACCAGAAAGCGATGCCTTCTCTGCTTTCCACTCTTCTTCTAACTCGGAGTACTGACGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCGATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCGTCAAGCGTCG  >  NC_000913/2732758‑2733233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑