Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,484,827 C→T L152F (CTT→TTT)  evgS → sensor histidine kinase EvgS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,484,8270CT93.8% 95.3 / NA 33L152F (CTT→TTT) evgSsensor histidine kinase EvgS
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (15/15);  minor base G (0/2);  total (15/18)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGT‑TATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAA‑CCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACTCACTCCTTAAATGTAGTG  >  NC_000913/2484591‑2485066
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                
ccGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGT‑TATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATg                                                                                                                                                                                                                                    <  1:12425/251‑1 (MQ=255)
           caacgtgctagcgtgaatAATGCTGATTATATAAAATTTGTTAAAAGAGCTTTAAATATCCAATTAGCACGACGGGAATACGCAGATCATCAAAAAGCAATGGACTCTCTTGCACAAGGTGACGTTGATATAG‑GTGTAACACACTTAGTTCCTTCGCCGCATCTTAATCACTCCAGTGCTGCCACCAAACCGTTGATAAGTCCCGTCCCGTTGCTGGTAACCACCCTTCACGACTCAATGCGTCCGCTTaa                                                                                                                                                                                                                         <  2:280819/234‑2 (MQ=255)
                              aTGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGT‑TATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAA‑CCAGTAAat                                                                                                                                                                                                     >  1:249681/1‑251 (MQ=255)
                                    attattTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGT‑TATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAA‑CCAGTCAATATTGcc                                                                                                                                                                                               >  2:129241/1‑250 (MQ=255)
                                          tAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGT‑TATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAA‑CCAGTAAATATTGCTCGGGt                                                                                                                                                                                          <  1:14165/250‑1 (MQ=255)
                                            aaTCTTTTAAAAAGATCGTTAAATATCAAATTAACACTCCGGGAATACGCAGCTCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGT‑TATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAA‑CCAGTAAATATTGCGCGGGTAGc                                                                                                                                                                                       >  1:459929/1‑251 (MQ=255)
                                                      aaaGTGCGCTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGT‑TATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAAGTCCCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAA‑CCAGTaaa                                                                                                                                                                                                      <  2:124754/226‑1 (MQ=255)
                                                      aaaGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGT‑TATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAA‑CCAGTaaa                                                                                                                                                                                                      >  1:124754/1‑226 (MQ=255)
                                                       aaGAGCGTTAAATAGCAAATGAACACTACGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCATAAGGTGAAGTTGATATAGTTT‑TATCACATTTAGTTACTGCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCTCCGTTCACGACGCAATGGGGCCGCTTACCTCACCAAAA‑CCAGTAAATATTGCTCGGGTAGCAAATTAccccc                                                                                                                                                                            <  1:550168/251‑1 (MQ=255)
                                                                    atCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGT‑TATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAA‑CCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAACCGAGGTAAt                                                                                                                                                               >  2:431604/1‑251 (MQ=255)
                                                                              cacTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGT‑TATCACAATTAGTTACTTAGCCGCCTCTTAATAATGAAATTGATGCAACAAAAACATTGATAATTCCCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGCCCGCTTACCTCACCAAAA‑ACAGTAAATATTGCTCGGGCAGACAATTATCCCACAGACGAGGTAATTCatcaatct                                                                                                                                                     >  2:318413/1‑250 (MQ=255)
                                                                                     ggAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGT‑TATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAA‑CCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCa                                                                                                                                               >  1:267887/1‑250 (MQ=255)
                                                                                           cGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGT‑TATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAA‑CCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGcaa                                                                                                                                        <  1:238710/251‑1 (MQ=255)
                                                                                              aGATCATCAAAAAGCAATGGACGCTCTGTCGGAAGGTGAAGTCGATATAGGGT‑TATCACCTTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTCCCTTTCCGGCGCTGGGATCCACCTTTCACGACACAATGCGAACGCTTACCTCACCAAAT‑CCAGTAAATATTGCTCGGGTAGCAAATTCCCCCCCAGACGAGGTAATTCATCAATCAGTTCCAAAAGcataa                                                                                                                                      <  2:443902/250‑4 (MQ=255)
                                                                                               gATCAGCAAAAAGCAATGGTAGGTCGTGCAGAAGGTGAAGTCGATATAGTGT‑TATCACCTGTAGTTACTTCGCCTCCTCTGAATAATAACAATGCTGCAACCAAACCAGTCATAATTTCCTTTCCGGCGCTGGTAACCGCCTTTCACGACTTAATGCGATCGCTTTCCTCACCAAAA‑CCAGTAAAAATTGCGCGGGTATCAAACTACCCCCCAGACGAGGTAATTCATCAATCATGTCCTAAATcaacaag                                                                                                                                    <  2:481894/251‑2 (MQ=255)
                                                                                                ataatcaTAAAGCAATGGACGCGCTTGAAGAAGGTGAAGTCTATATAGTTT‑TATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAA‑CCAGTAAATATTGCTCAGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAAtt                                                                                                                                   <  2:311414/248‑1 (MQ=255)
                                                                                                     caAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGT‑TATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAtct                                                                                                                               <  1:140037/251‑1 (MQ=255)
                                                                                                            cAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGT‑TATCACATTTAGTTACTTCGCCGACTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCAGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCATCCCTCACCAAAA‑CCAGTAAATATTGATCGGGTAGCAAATTACACCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTAc                                                                                                                        >  1:56755/1‑250 (MQ=255)
                                                                                                                                                          aTTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAA‑CCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGAt                                                                          <  1:6520/251‑1 (MQ=255)
                                                                                                                                                            ttAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAA‑CCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGAtt                                                                         >  1:94352/1‑250 (MQ=255)
                                                                                                                                                                                      tGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGGAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAA‑CCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTATGCACAATGATTACTTTATTGGTAGTAACATCATTAcc                                               >  1:42914/1‑250 (MQ=255)
                                                                                                                                                                                       gACATTGCTGCAACCAAACCATTGATAAGTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAA‑CCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTaaca                                              <  2:249681/250‑4 (MQ=255)
                                                                                                                                                                                               tgcAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAA‑CCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAACTTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATgt                                     >  2:200337/1‑250 (MQ=255)
                                                                                                                                                                                                              gATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAA‑CCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATTATTTCCCGCTATTtc                       <  1:487399/250‑1 (MQ=255)
                                                                                                                                                                                                                gaaTTTCCTTTCCGGCGCTGTGAACCACCTTTCTCGACGTAATGCGAACGCTTACCTCACCAAAA‑CCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAAATCATCAAGCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGACTACTTTATTGGTAGTAACATCATGACCAGCAGTATGATTTCCTGCTATTtcact                    <  2:63533/250‑1 (MQ=255)
                                                                                                                                                                                                                  aTTACCTTTCCGGCGCTGGGAACCACCGTTCACGCCTCTATGCGACCGCTTACCTCACCAAAA‑CCAGTAAATATTGCGCGGGTAGCAAATTACCCCCCAGACGAGGTAAGTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATTATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTtcactca                  <  2:305382/251‑1 (MQ=255)
                                                                                                                                                                                                                     aCCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAA‑CCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTtcact                    >  1:63533/1‑246 (MQ=255)
                                                                                                                                                                                                                     aCCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAA‑CCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCactcactc               <  1:129241/251‑1 (MQ=255)
                                                                                                                                                                                                                      ccTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAA‑CCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATcc                                                                                               >  2:264110/1‑170 (MQ=255)
                                                                                                                                                                                                                      ccTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAA‑CCAGTAAATATTGCGCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATcc                                                                                               <  1:264110/170‑1 (MQ=255)
                                                                                                                                                                                                                              gcgcTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAA‑CCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACTCACTCCTTAAATGt      <  1:200337/251‑1 (MQ=255)
                                                                                                                                                                                                                               cgcTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAA‑CCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACTCACTACTTAAATGt      >  1:346142/1‑250 (MQ=255)
                                                                                                                                                                                                                                   ggTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAA‑CCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACTCACTCCTTAAATGAAGTg  >  2:449451/1‑250 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                
CCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGT‑TATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAA‑CCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACTCACTCCTTAAATGTAGTG  >  NC_000913/2484591‑2485066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑