Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,384,808 C→T K305K (AAG→AAA)  yfbK ← IPR002035/DUF3520 domain‑containing protein YfbK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,384,8080CT95.1% 124.8 / ‑3.5 43K305K (AAG→AAA) yfbKIPR002035/DUF3520 domain‑containing protein YfbK
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (26/13);  total (28/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

TCTGCGCTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGG‑CTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTC‑GTC‑GCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTTCATCAGAAATCATTG  >  NC_000913/2384573‑2385046
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                
tCTGCGCTTAAGAGAGGGTATCAATGTATATGTAGTTGCCGTTACCAACATCGGCAATTAGCACAATCAATGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGCCGACAGAGTAACACCAGACTCACGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTAAATGCCCACGTTAAAGTCAACGTCAATGG‑CTATTAAAATGCGATTGATGCCGCCATTAATAACACCTTTC‑GCC‑GCctgctg                                                                                                                                                                                                                                  >  2:569904/1‑250 (MQ=255)
      cTTCAGAGAGGGAATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGG‑CTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTC‑GCCTGCTGATAAGcc                                                                                                                                                                                                                           >  1:141707/1‑251 (MQ=255)
        tCAGAGAGGGGATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGG‑CTAATAAAATGCGATTGATGCCGCCCTTAATAACCCCTTTC‑GTC‑GCCTGCTGATAAGcc                                                                                                                                                                                                                           >  2:73471/1‑249 (MQ=255)
                    tCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGG‑CTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTC‑GCCTGCTGATAAGCCAGTTCCAGCCCGGc                                                                                                                                                                                                             >  1:212709/1‑251 (MQ=255)
                     cAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCTCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGCTTCAATCGATTTTGGATCGTCAATGCCAACGTTACAGTCACCGACACAGG‑CTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTC‑GACTACTGATAAGCCAGTTCCAGCCCgga                                                                                                                                                                                                             >  2:369719/1‑249 (MQ=255)
                         gTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGCGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCCATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGG‑CTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTC‑GCCTGCTGTTAAGCCCGTTCCAGACCGACAcccc                                                                                                                                                                                                        >  2:243346/1‑249 (MQ=255)
                                      cagTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCACACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGG‑CTAATAAAATGCGCTTGATGCCGCCCTTAATAAACCCTTTC‑GTC‑GCCTGCTGATAAGCCCGTTCCAGCCCGACACCGCCATTGGTACTGcc                                                                                                                                                                                           >  2:110557/3‑251 (MQ=255)
                                         ttACCAACACCGGCAATTCGCACCATCATTGCCTCGTTGTAAGTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGG‑CTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTC‑GCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCtt                                                                                                                                                                                         <  1:369719/250‑1 (MQ=255)
                                          tACCACCATCGGCAATTCGCCCCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGG‑CTAATAAAATGCGATTGATGCCGCCCTTAATAACCCCTTTC‑GTC‑GCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTc                                                                                                                                                                                        <  1:305206/250‑1 (MQ=255)
                                          tACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGG‑CTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTtc‑gtc                                                                                                                                                                                                                                           <  1:245334/200‑1 (MQ=255)
                                          tACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGG‑CTAATAAAATGCGATTGATGACGCCCTTAATAAACCCTTtc‑gtc                                                                                                                                                                                                                                           >  2:245334/1‑200 (MQ=255)
                                              aaCAGCGGCAATTCCCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACCCCAACCGTCGACAGAGTAACACCAGACGCCCGCGGTTTTGTGACCATGTATTCAATCGATTTTGGATCGACAATGCCAACGTTAAAGTCACCGTCAGTGG‑CTAATAAAATGCGATTGATGCCCCCCTAAATAAACCCTTTC‑GTC‑GCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCAt                                                                                                                                                                                   <  1:23295/251‑1 (MQ=255)
                                                                     ttGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGG‑CTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTC‑GCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCAtt                                                                                                                                                             >  1:466582/1‑250 (MQ=255)
                                                                         cccgttcagttttccaTTCCCCACGCCAAACGTCGACAGAGTAACTCCAGACTCCCGCTGGTTTTTACCCATTGTTTCAATCGATTTTGGATCTTCAATGCCAACGTTAATGTCTCCTTCAGTGT‑CCTATAAAATGCGATTGATTCCGCCCTTAATAACCCCTTTC‑GTC‑GCCTGCTGATAAGCCAGTTCCAGCCCGTCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATGGCGGCATTAAttt                                                                                                                                                        <  1:438309/236‑1 (MQ=255)
                                                                         cactttGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATCTAATCGTTTTTGGATCGTCAATGCCAACGTTAAAGTCCCCGTCAGTGG‑CTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTC‑GCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAAttg                                                                                                                                                        <  1:438292/247‑2 (MQ=255)
                                                                         cTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGG‑CTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTC‑GCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAAttt                                                                                                                                                        >  1:231985/1‑251 (MQ=255)
                                                                            gTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGG‑CTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTC‑GCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCg                                                                                                                                                     >  1:483084/1‑251 (MQ=255)
                                                                                tAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGG‑CTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTC‑GCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCCTTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCtt                                                                                                                                                 >  2:7561/1‑251 (MQ=255)
                                                                                             ccACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGG‑CTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTC‑GCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCgg                                                                                                                                      >  1:72329/1‑249 (MQ=255)
                                                                                             ccACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGG‑CTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTC‑GCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCgg                                                                                                                                      >  1:15503/1‑249 (MQ=255)
                                                                                               aCGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGG‑CTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTC‑GCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCgg                                                                                                                                      >  1:232777/1‑247 (MQ=255)
                                                                                                       cGTCGACAGAGTAACACCAGACTCCCGCTTTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGG‑CTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTC‑GCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGgca                                                                                                                          <  1:220399/251‑1 (MQ=255)
                                                                                                                agTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGG‑CTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTC‑GCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATAc                                                                                                                 <  1:467724/251‑1 (MQ=255)
                                                                                                                  tAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGG‑CTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTC‑GCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCAAGCGAATCAATTGCGGCCTTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACgg                                                                                                               >  1:258986/1‑251 (MQ=255)
                                                                                                                                     ttttttGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAACGTCACCGTCAGTGG‑CTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTC‑GCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAAGTGCGGCATTAATTTCCGCCTTATGACGCCCGGAGAGAGACGGCAATGCAATAAGGGGGTCGCAAGCGTAggac                                                                                             >  2:246804/1‑248 (MQ=255)
                                                                                                                                       ttttGACCATTGATTCAATCAATTTTGGTTCGTAAAAGCAAACTTTAAAGCCACTGTCAGTGG‑CTAAAAAAATGCGTTTGATGCCGCCATTAATAAACCCTTTC‑GGC‑GCCTGCTGATAAGCCAGTTCCATCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCAc                                                                                           <  2:212709/250‑1 (MQ=255)
                                                                                                                                          taaCCATTGAGTCAATCGATTTTGGATCGTAAATGCAAACGTTAAAGTCACAGTCAGTGG‑CTAATAAAATGCGAATGATGCCGCCCTTAATAAAACCATTC‑GTC‑GACTGCTGATAAGCCAGTTCCAGACCGGCACCGCTATTGGGAATGCCTTCGGCATCAGGAGAATCAATTGCGGCATTAATTTCCGCCTTAAGACCACCGGAGATGGAAGGCAAGGCAAAACGGGAAGCGCCAGCGGAGGTAACGATg                                                                                       >  2:151567/3‑251 (MQ=255)
                                                                                                                                                    ttCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGG‑CTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTC‑GCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTAt                                                                             >  1:152614/1‑251 (MQ=255)
                                                                                                                                                           gATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGG‑CTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTC‑GCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCgg                                                                                                                                      >  2:468682/1‑187 (MQ=255)
                                                                                                                                                           gATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGG‑CTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTC‑GCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCgg                                                                                                                                      <  1:468682/187‑1 (MQ=255)
                                                                                                                                                             ttttGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGG‑CTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTC‑GCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCAc                                                                    >  1:22283/1‑251 (MQ=255)
                                                                                                                                                                      gtcaagtcaacagctAAAGTCCCCGTC‑GTGGGCTAATAAAATGCGATGTATGCCGCCCTTAATAA‑CCCCTTCCGTC‑GCCTGCTGAAAAGCCAGTTCCAGCCCGGCCCCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCtt                                                           <  2:231985/237‑1 (MQ=255)
                                                                                                                                                                                    tAAAGTCACCGTCAGTGG‑CTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTC‑GCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAAttt                                              <  1:461848/250‑1 (MQ=255)
                                                                                                                                                                                                   tGG‑CTAATAAAATGCGATTGATGCCGCCCTTAATCAACCCTTTC‑GTC‑GCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCCATGTTATCCTGCTCACGAAGTTCTTTGCCCAATAATTTCAACGAAGACTGGAt                               >  2:94643/1‑250 (MQ=255)
                                                                                                                                                                                                     g‑CTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTC‑GCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATa                              >  1:344886/1‑249 (MQ=255)
                                                                                                                                                                                                        taataaAATGCGATTGATGCCGCCCTTAATAAACCT‑TTC‑CTCCGCTTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGt                           <  1:552713/250‑1 (MQ=255)
                                                                                                                                                                                                         aataaAATGCGATTGTGGCCGCCCGTAATAATCCCTTTC‑TTC‑GGCTGTTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGTGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGt                           <  1:331621/249‑1 (MQ=255)
                                                                                                                                                                                                         aaaaaaaTGCGATGGTGGCCCCCCTTAATAACCCCTTTC‑GTC‑GCTTGATGATAAGCCAGTCCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGt                           <  1:12092/246‑1 (MQ=255)
                                                                                                                                                                                                               aTGCGATTGATGCCGCCCTTAATAAACCCTTTC‑GTC‑GCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACg                   >  1:439366/1‑251 (MQ=255)
                                                                                                                                                                                                                        ctGCCGCCCTTAATAAACCCTTTC‑GAC‑GACTGCTGCTAAGCCCGTTCCCGCCCGGCACCGCCATTGGGACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGCTAGCAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTTACCAATAATTTCAACGAAGCCTGGATAAGGTGCAAACGTacatc             >  2:18085/2‑248 (MQ=255)
                                                                                                                                                                                                                              cccTTAATAAACCCTTTC‑GTC‑GCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTTCATCAGAAATCAt    >  1:26051/1‑251 (MQ=255)
                                                                                                                                                                                                                              cccTTAATAAACCCCTTC‑GTC‑GCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTTCATCAGAAATCAt    >  1:138536/1‑251 (MQ=255)
                                                                                                                                                                                                                                 ttAATAAACCCTTTC‑GTC‑GCCTGCTGCTAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTACCTTCGGCATCCAGCGACTCACTTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGCGCCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGCAGACTGGATAAGTGGCACACGTTCATCAGAAATCATTg  >  2:168548/1‑250 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                
TCTGCGCTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGG‑CTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTC‑GTC‑GCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTTCATCAGAAATCATTG  >  NC_000913/2384573‑2385046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑