Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,134,498	G→A	V385V (GTC→GTT)	wzc ←	protein‑tyrosine kinase Wzc

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,134,498	0	G	A	100.0%	147.4 / NA	49	V385V (GTC→GTT)	wzc	protein‑tyrosine kinase Wzc
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/22);  total (27/22)

TAATACCGTGTTCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAGACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTC  >  NC_000913/2134260‑2134740                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   taataaCGTGTTCTTCCAGAACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCAGGGTCAACAATGCGCACATCGCCGACGGTGCTGGGCTCGGTGATTTTCAGCTCCTGCTCTTTATTCCACAGTTGCATATAAACctgctgcccc                                                                                                                                                                                                                                        >  2:234158/1‑247 (MQ=255)               tcCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATcgcg                                                                                                                                                                                                                          >  1:1847/1‑251 (MQ=255)                   gTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTc                                                                                                                                                                                                                      <  1:27504/251‑1 (MQ=255)                           gggCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCaaccgaac                                                                                                                                                                                                              >  2:204888/1‑244 (MQ=255)                                ttCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCCGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTAAGACGGACAAtcc                                                                                                                                                                                                         >  2:501196/1‑250 (MQ=255)                                 tccaTGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTAAGCACCTGCTCTTTATTCAGCCGTTGCATATCAACCTGCTGACCCGACTAGACATCGCGGGTCAGACGGACAAtctc                                                                                                                                                                                                        >  2:561194/4‑251 (MQ=255)                                     agCCGCGATTAAACAACCAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCGTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCctgc                                                                                                                                                                                                    <  2:266388/250‑1 (MQ=255)                                                  caacGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCa                                                                                                                                                                                       <  1:501196/251‑1 (MQ=255)                                                  caacGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCa                                                                                                                                                                                       <  1:46765/251‑1 (MQ=255)                                                   tacGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGAACTCGCGGGTCAGACGGACAATCTCCTGCTGGTTTTTCGGCAt                                                                                                                                                                                      >  2:112966/2‑251 (MQ=255)                                                        gcgcAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTGCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCg                                                                                                                                                                                 <  2:136990/251‑1 (MQ=255)                                                          gcagcagcaCACCCACGATAGAAAGCATCAGGCCAAGGAAAATCGAGACGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGAATCGTAATCGCGAGTCAGACGGACAATCTCCTGCTGGGGTTGCGGCATCGCCGtt                                                                                                                                                                               >  2:490960/1‑251 (MQ=255)                                                             gcagcaCACCCACGAGAGAAAGCATCAGGCCAAGGAAAATCGCGCCGAGGATAATCAGCCCTTGCCTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTAcg                                                                                                                                                                            <  2:1847/251‑1 (MQ=255)                                                                 cacaCCCACGATAGAAAGCATTAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCTTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATGGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGATGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGAc                                                                                                                                                                        <  2:484820/251‑1 (MQ=255)                                                                 aacacCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCCCGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACACCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCGGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGAc                                                                                                                                                                        <  2:192088/250‑1 (MQ=255)                                                                   cacctactaTAGAAAGCAGCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGGTTCAGCACGCCAGGCTGAGTGATTGCCGGTTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTGCAGCTCCTTCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCTCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGAcag                                                                                                                                                                      <  2:87861/243‑3 (MQ=255)                                                                    acCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACCATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGCCCGt                                                                                                                                                                     >  1:69761/1‑251 (MQ=255)                                                                      ccACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTa                                                                                                                                                                   <  1:294294/251‑1 (MQ=255)                                                                         cGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCCGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGCCTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGt                                                                                                                                                                >  2:286449/1‑251 (MQ=255)                                                                                           cAAGGATAATCGCGCCGAGGCTAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGAAGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTcc                                                                                                                                              >  2:168215/1‑251 (MQ=255)                                                                                                 tAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGCGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGcc                                                                                                                                        >  2:275639/1‑251 (MQ=255)                                                                                                  aaTCGCGCCGAGGATAAGTAGCCATTTCGTCGGGTTCAGCACGCCAGGCTGAGTGATGGCCGTGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTGTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCt                                                                                                                                       <  2:125180/251‑1 (MQ=255)                                                                                                              gATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCGTGACCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTTCTGGGTTTTCGGCATAGCCGTTACACGACCGTTAAGTTTGGCTTTTTCGTATTCCATCGCCTGACGTTTCTcc                                                                                                                            >  2:510614/1‑250 (MQ=255)                                                                                                              gATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGCATCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCATCGCCGTACGTTTCTccc                                                                                                                           >  2:475084/1‑250 (MQ=255)                                                                                                               aTAATCAGCCCTTTCTTCGGTTTCAGCACGCAAGGCTGAGTGATTGCCGGGTCAACACTGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCTTCATCGCGGATCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGGTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTcc                                                                                                                            >  1:150162/1‑249 (MQ=255)                                                                                                                     aGCCCTTTCTTCGTTTTCAGCACGACAGGCTGAGTGATTGCCGAGTCAACAATGCGCACATCGACGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACATGCTGACCAGACTCGACATCGCGGGTCAGCCGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGGTCAGCGACCGTTAAGTTTGTCTTTTTCGTCTTCCAGCGCCTGACCTTTCTCaagcatc                                                                                                                      >  2:290335/1‑247 (MQ=255)                                                                                                                            tcttcGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGt                                                                                                                                                                     <  1:466464/195‑1 (MQ=255)                                                                                                                            tcttcGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTACGCTCCTGCGCTTTATTCAGCAGTTGCATATAACCCTGCTGACCAGACTCGCCATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGt                                                                                                                                                                     >  2:466464/1‑195 (MQ=255)                                                                                                                              ttcGGTTGCACCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACCTCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCCCTTTAGTCAGCAGGTGCATATAAACCTGTTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACg                                                                                                            <  2:22050/250‑1 (MQ=255)                                                                                                                                   tttCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGAGGTACGCCgggg                                                                                                      >  2:70385/1‑250 (MQ=255)                                                                                                                                             ccAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTAcgc                                                                                                                                                                           <  2:216352/172‑1 (MQ=255)                                                                                                                                             ccAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTAcgc                                                                                                                                                                           >  1:216352/1‑172 (MQ=255)                                                                                                                                                   tGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTAc                                                                                        >  1:323017/1‑249 (MQ=255)                                                                                                                                                         aTTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTggg                                                                                >  2:162102/1‑250 (MQ=255)                                                                                                                                                            gccggttaaacaaTGCGCCCATCACCGGCAGTTCTGGCCTCGGTGATTGTCAGCTCATTCCCTTTACTCATCAGTAGCATAGAAACCGGCTGGCCAGACTCGGCATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTACGGCATCGCCGTTACGCGACCGTTAAGTTTGTCTTTTTCGTCTTCCAGCGCCTGACGTATCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGAt                                                                             <  2:142744/243‑1 (MQ=255)                                                                                                                                                                    aacaaTGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCct                                                                     <  1:162102/251‑1 (MQ=255)                                                                                                                                                                        aTGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTGTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCttt                                                                 <  1:376600/251‑1 (MQ=255)                                                                                                                                                                             cacaTCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTAcgcg                                                                                                                                                                          <  1:383555/141‑1 (MQ=255)                                                                                                                                                                             cacaTAGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTAcgcg                                                                                                                                                                          >  2:383555/1‑141 (MQ=255)                                                                                                                                                                                        cGGTGCTGACCTCGGTGATTTTCAGCTCATGCTCTTTATTCAGAAGTTGCATCTAAACCTGCTGCCCAGACTCGACATCGCGGCTCAGACGGCAAATCACATGCTGGGATTTCGGCATCGCCGTTACGCGCCCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCCGCAGCGTGCGGTACGCCGGGAGAGCTTTGGTGAACAGCTTAGCGATTTTCGCCCCTTTAAAggcaaattcgttc                                                 >  2:278725/1‑240 (MQ=255)                                                                                                                                                                                                        gATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGt                                 <  1:275639/251‑1 (MQ=255)                                                                                                                                                                                                        gATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGt                                 <  1:204888/251‑1 (MQ=255)                                                                                                                                                                                                         aTTTTCAGCTCCTGCTCGTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTTGTTGACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGt                                 <  1:317689/250‑1 (MQ=255)                                                                                                                                                                                                           tttCAGCGCCTGCTCTTTATTCAGCCGTTGCATATAAACATGCTTACCAGACTCGGCTTCTCTGGTCATACGGACAATCTCCTGCTGGGTTTTCGTCCTCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCGGCGCCTGACGTTTCTCCAGCAGCGTGCGTGACTCCTGGTGAACTTTGGTGTCCCGCTTGGTTATTTCCTCCTCTTTAACTGTCAATACGTTCCTCTACGCATCGGTGTTc                               >  2:144469/1‑250 (MQ=255)                                                                                                                                                                                                                gccccTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCatc                         <  1:50692/248‑1 (MQ=255)                                                                                                                                                                                                                       ctctTTATTCAGCCGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCatcgaatcg                   >  2:526357/1‑250 (MQ=255)                                                                                                                                                                                                                                      ttGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCTGGTGACCTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGAATCGATGTTCACCATCGAATCGAGCCCCGCTTTTGCtt   >  2:299466/1‑251 (MQ=255)                                                                                                                                                                                                                                      ttGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCtt   >  1:435882/1‑251 (MQ=255)                                                                                                                                                                                                                                       tGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTc  >  1:570241/1‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   TAATACCGTGTTCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAGACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTC  >  NC_000913/2134260‑2134740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑