Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,103,891	G→A	P207S (CCA→TCA)	wbbK ←	putative glycosyltransferase WbbK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,103,891	0	G	A	100.0%	42.6 / NA	17	P207S (CCA→TCA)	wbbK	putative glycosyltransferase WbbK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/6);  total (11/6)

CTAGAGGGAAAACAAACTATATCTGAAATATTATAACAATGATCGATTTTTTCTTTATCCAAGTACCCGAGGAAATGAACATTATCCAGTCCTTCTGCAAGACTGATAATATATTTTGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGGAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCC  >  NC_000913/2103665‑2104135                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     cTAGAGGGAAAAAAAACTATATCTGAAATATTATTACAAAGATCGATTTTTTCTTTATCAAAGAACCCGAGGAAATGAACATTATCCAGTACTTCTGCAAGACTGATAATATATTTTGCATACGCATTTTCTGTAACACTGATAGTAATAAGAAATTTAATATTGGCTTGTTCTTTCAATTTCCTTGCTGAACTAATAATAAGCTCGTAATTTTTAAATACTCGTAAAACAGCAGGGTAAAATATTGTCa                                                                                                                                                                                                                               >  2:356658/1‑250 (MQ=255)                    tatCTGAAATATTATAACAATGATCGATTTTTTCTTTATCCAAGTACCCGAGGAACTGAACATTATCCAGTCCTTCTGCAAGACTGATAATATATTTTGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCtt                                                                                                                                                                                                            >  1:265464/1‑250 (MQ=255)                                      aaTGAACGATTTTTTCTTTATCCAAGTACCCGAGGAAATGAACATTATCCAGTCCTTCTGCAAGACTGATAATATATTTTGCATACGAATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAAAAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAatcatcatc                                                                                                                                                                                          >  1:340960/1‑250 (MQ=255)                                        tGATCGCTTTTTTCTTTATCCAAGTACCCGAGGAAATGAACATTATCCAGTCCTTCTGCAAGACTGATACTATATTTTGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATACGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCCACTCAGTAGGGTTATTCTTAAATGTAGAATCTTCATCAGt                                                                                                                                                                                       >  2:164812/1‑251 (MQ=255)                                                   ttCTTTATCCAAGTACCCGAGGAAATGAACATTATCCAGTCCTTCTGCAAGACTGATAATATATTTTGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTACTTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCttt                                                                                                                                                                            >  2:222715/1‑251 (MQ=255)                                                   ttCTTTATCCAAGTACCCGAGGAAATGAACATTATCCAGTCCTTCTGCAAGACTGATAATATATTTTGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCttt                                                                                                                                                                            >  1:238042/1‑251 (MQ=255)                                                                   ccGAGGAAATGAACATTATCCAGTCCTTCTGCAAGACTGATAATATATTTTGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGAtaatttaat                                                                                                                                                            <  2:2683/251‑1 (MQ=255)                                                                                                            aataTATTTTGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGTGTAAAATATTGTCAACTCAGAATGGTTATTCTTAAATTGAGAATCAGCATCAGTAAGTTGGCTTTGATCAGATAATTGAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCt                                                                                                                   <  1:356658/251‑1 (MQ=255)                                                                                                                                       aCCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGc                                                                                                                                                                               >  1:343237/1‑164 (MQ=255)                                                                                                                                       aCCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCCTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGc                                                                                                                                                                               <  2:343237/164‑1 (MQ=255)                                                                                                                                             gATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGGAATTTTTAAATACTCGTGAAACAGCATGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTGATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGaa                                                                                  <  2:265464/251‑1 (MQ=255)                                                                                                                                                   taGCAGAAATTTAATATTGGATTGTTCTTTCAATTTACTTGCTACACTAATAATAAGCTCGTACTTTTTAATTACTCGTGAAACAGCAGGGTAAAATATTGTCAACTAAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGACCGACTGACAATGATGTTATTTATAGTATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGaacaaacc                                                                            >  2:214817/2‑250 (MQ=255)                                                                                                                                                               aaTATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTAttt                                                                 <  1:164812/250‑1 (MQ=255)                                                                                                                                                                                               cACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAg                                                                                                                            <  1:210345/159‑1 (MQ=255)                                                                                                                                                                                               cACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAg                                                                                                                            >  2:210345/1‑159 (MQ=255)                                                                                                                                                                                                         aaGCTCGGAATTTTTAAATACTCGTGAAACAGCAGGGTAACATATAGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATAAATCAGCCCGTATAGCATTTTa                       >  2:326919/1‑250 (MQ=255)                                                                                                                                                                                                                             tacGTGAAACAGCAGGGTAAAATATTGTCAAATAAGTAGGGTTATTCTTAAATTGAGAATCAACATCCGTAAGTTGGCTTTTATAAGATAATTTAATTTATGGCCGACTGACAATGATGTTATTTATAGAATCTTTCTTGATACATTTTTATTTCATCCAGAATTATTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGACCGTATAGCATTTTAAATAAGAAAAATCTAGACTcc  >  2:486570/3‑251 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     CTAGAGGGAAAACAAACTATATCTGAAATATTATAACAATGATCGATTTTTTCTTTATCCAAGTACCCGAGGAAATGAACATTATCCAGTCCTTCTGCAAGACTGATAATATATTTTGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGGAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCC  >  NC_000913/2103665‑2104135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑