Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,085,319	G→A	A69A (GCC→GCT)	tsuA ←	thiosulfate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,085,319	0	G	A	100.0%	79.4 / NA	26	A69A (GCC→GCT)	tsuA	thiosulfate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/12);  total (14/12)

CAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAG‑CGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCGGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGG‑CAAAAACGCCCACGCTGCATGACAAATCCCAGCAGAGCACCACAAATTAGCCCGCTTAATATCATTGAAAACATAAATTAATTAACCAGATGAATGTTA  >  NC_000913/2085073‑2085550                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                         aaaaTTAAAAGTCTCAGCAATAGAGTTATGTTCATTACTGTAGTGCTGAAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCACCCCCATATAAGTGAAAAG‑CGCGATCCAACTGCCGATCAACCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCtt                                                                                                                                                                                                                                      <  1:111445/250‑1 (MQ=255)        aaaGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAG‑CGCGATCCAACTGCCGATCAATCCCTCACCCGAGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGACGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGt                                                                                                                                                                                                                               >  1:551213/1‑251 (MQ=255)                    aTAGAGTTATTTTCAGTACTGTAGTGCTGCAAGGTTTAATTTAAACCACTGGCATGTGGAGAACGCATCACCGCCCTCATCCCCATAAAAGTGAAAAG‑CGCGATCAAACTCCCGATCAACCCCTCCCCCGCGCGATCCCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGc                                                                                                                                                                                                                   <  1:292668/251‑1 (MQ=255)                                 cAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGCGAACGCATCACCGCACTCATCACCATATAAGTGAAAAG‑CGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGACGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCATAAGTCAGTAGGCCCGCCTCAATTaaacc                                                                                                                                                                                                       >  2:249762/1‑247 (MQ=255)                                            tgctgcAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAG‑CGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCAAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCAc                                                                                                                                                                                           >  1:335314/1‑251 (MQ=255)                                                         aattaaaaCCACGGGAATGGGGAGAACGCATCACCTCACTCATCACCAAAAAATTGAAAAG‑CGCCATCCAACTGCCTAACAATCCCCCACCCGCGCGATACCAGGTCCGGGTGTCACATCCACCCGCAAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAAGCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGaa                                                                                                                                                                              <  1:459330/246‑1 (MQ=255)                                                             aaacccccgggCATGTGGAGACCGCATCCCCGCACTCACCACCATATAAGTGAAAAG‑CGCGATCCACCTGCCGATCAACCCCCCCCCCGCGCGATACCAGGTCCCGGGGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTg                                                                                                                                                                          <  1:290846/242‑1 (MQ=255)                                                              aaaCCACTGGCATGTGGAGACCGCATCACCGCCCTCAACACCATCTAAGTAAAAAA‑CGAGATCCAAATGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCAGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATCTACCCACCTATAACAGTACCAAGCCAAGAGAACGCCCCAGCTTCGTACGTCAGTAGGACAGCCTGACTTACCGAAAAAACCCCCACGCTTTGAACCgccattac                                                                                                                                                                         >  2:227100/1‑244 (MQ=255)                                                                   ccTGGCATGGGGAGACCGCATCCCCGCACTCTTCACCATATAAGTAAAAAG‑CGCGATCCAACGGCCGACCAACCCCTCCCCCGCGCGATACCAGGCCCCGGGGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCa                                                                                                                                                                    <  1:259404/250‑1 (MQ=255)                                                                                 aaCGCATCACCGCACTCATCACCATATAAGTGAAAAG‑CGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAAc                                                                                                                                                      >  1:67029/1‑251 (MQ=255)                                                                                       tCACCGCACTCATCACCATATAAGTGAAAAG‑CGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCCCGCTTTGTACCGAAATTGCAATCAGCAGGGCGTAAAACATg                                                                                                                                                   >  2:496753/1‑248 (MQ=255)                                                                                             cACTCATCACCATATAAGTGAAAAG‑CGCGATCCAACTGCCGATCAATCCCTCACACGCGCGATACAAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCAAGCTTCGTACGTCAGTAGGGCAGCCTGACTTACCGCAAAAACCCCCACGCTTTGTACAGAAATTGCAACCAGCAAGGCGTAAAACaagatattattt                                                                                                                                          >  2:253166/1‑240 (MQ=255)                                                                                              ccctcaccccaataaaatGAAAAGGCGCGATCCACCTGCCGATCAACCCCTCACCCGCGCGATACCGGGCCCGGGTGCCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTAttt                                                                                                                                          <  1:13992/234‑1 (MQ=255)                                                                                                  acccccATATAAGTGAAAAG‑CGCGACCCAACTGCCGACCAACCCCCCCCCCGGGGGATCACAGGCCCCGGTGGCACACCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCAc                                                                                                                                     <  1:8445/247‑1 (MQ=255)                                                                                                              gTGAAACG‑CGCGATGCAAATGCCGATCAATCCCTCACCAGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCACCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATc                                                                                                                         >  2:340591/1‑251 (MQ=255)                                                                                                                                               ccccccgtgcacaTACCAGGTCCGGGTGCCACATCCACCCGCCAGAACAATGCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATGAACGCAAAAACCCCCACTCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGG‑CAAAAACGCCCAc                                                                                        <  1:564088/239‑1 (MQ=255)                                                                                                                                                    ccgcacacactcAGGTCCCGGTGCCACACCCCCACTCCAGAAAAATTCCCAGCCCGAAGATATACCCACCGATAACAATACCAAACCCCGGTAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTTAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACAGGCGATTATTTTTCACGATATACATTTCGCGAAAACCACCTGTCAGG‑CAAAAACGCCCACGCTGc                                                                                   <  2:527309/240‑1 (MQ=255)                                                                                                                                                                aGGTCCCGGTGGCACACCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGG‑CAAAAACGCCCACGCTGCATGACAAATccc                                                                       <  1:559535/251‑1 (MQ=255)                                                                                                                                                                    cccGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGG‑CAAAAACGCCCACGCTGCATGACAAATCCcagc                                                                    >  1:53624/1‑250 (MQ=255)                                                                                                                                                                                tCCACCCGCCAGCACAATTCCCAACCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGACACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCAGAATTAACGCAAAAACCCCCACGCTTTGTACCGTAATTGCAATCAGCACTGCGAAAAACATGCGATTATTTTCCCCGCTATACATATCGCGAAAACCACCTGCCAGAACAAAAA‑GCCACCGCTACATGACAAATCCAAACAAAACACCACaa                                                        >  2:397233/1‑250 (MQ=255)                                                                                                                                                                                                               atatACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGG‑CAAAAACGCCCACGCTGCATGACAAATCACAGCAGAGCACCACAAATTAGCCCGCTTAATATCATTGAAAACATaa                         >  1:429477/1‑250 (MQ=255)                                                                                                                                                                                                                tataCCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGG‑CAAAAACGCCCACGCTGCATGACAAATCCCAGCAGAGCACCACAAATTAGCCCGCTTAATATCATTGAAAACATAaat                       >  1:267418/1‑251 (MQ=255)                                                                                                                                                                                                                aatccccACCAATAACATGACCAAGCCACGGGAACGCCCCAGCTTCGAAAATCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGG‑CAAAAACGCCCACGCTGCATGACAAATCCCATCAGAGCACCACAAATTAGCCCGCTTAATATCATTGAAAACATAaat                       <  2:53624/247‑1 (MQ=255)                                                                                                                                                                                                                                  tACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGG‑CAAAAACGCCCACGCTGCATGACAAATCCCAGCAGAGCACCACAAATTAGCCCGCTTAATATCATTGAAAACATAAATTAATTAACCAGATGAATg     >  1:137677/1‑251 (MQ=255)                                                                                                                                                                                                                                   aCCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGAAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGG‑CAAAAACGCCCACGCTGCATGACAAATCCCAGCAGAGCACCACAAATTAGCCCGCTTAATATCATTGAAAACATAAATTAATTAACCAGATGAATGt    >  1:224362/1‑251 (MQ=255)                                                                                                                                                                                                                                     cAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGG‑CAAAAACGCCCACGCTGCATGACAAATCCCAGCAGAGCACCACAAATTAGCCCGCTTAATATCATTGAAAACATAAATTAATTAACCAGATGAATGTTa  <  1:496753/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                         CAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAG‑CGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCGGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGG‑CAAAAACGCCCACGCTGCATGACAAATCCCAGCAGAGCACCACAAATTAGCCCGCTTAATATCATTGAAAACATAAATTAATTAACCAGATGAATGTTA  >  NC_000913/2085073‑2085550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑