Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,080,405	C→T	G63S (GGT→AGT)	yeeA ←	putative transporter YeeA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,080,405	0	C	T	92.0%	73.2 / NA	25	G63S (GGT→AGT)	yeeA	putative transporter YeeA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (11/12);  minor base G (0/2);  total (11/14)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

ACGCCGTATCAATTTCACCTGTCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCAC‑CAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATACCCGGATTTCAAACGGGCTTAATGACTTATCGGCACGCACGCAGAAACTCCGTAATGAGATTAAAAGCGCTCATAAC  >  NC_000913/2080168‑2080628                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                aCGCCGTACAAATTCCACCTGTCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCAC‑CAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGAtt                                                                                                                                                                                                                    <  1:563468/251‑1 (MQ=255)                  ccTGTCGGGGAACCCACAACAATTGCCCGCGTCACCCCAATCAATAAAACTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCCAAGGAACATGGCCGCCGCGCAC‑CAGATTAACATCAGCGGTACCGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTAGCCCCATAATcaccac                                                                                                                                                                                                   >  1:79287/1‑251 (MQ=255)                    tGTCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATCCGGTTTCTTGCCCAGCGCACGCCAACAGCAAAGGAACATGGCCGCCGCGCAC‑CAGACTAACAACAGCGGAAAAGTGATTAACTCCAGTTGCAGAGCGATAAGAACTAAAATCACACACAAAACCGGACCGCCAATACGATCAAAGGCACGAGGGACAACGTTAATCCAGAACGAGCAAGGCCCCATAATcaccaac                                                                                                                                                                                                  >  2:551358/1‑248 (MQ=255)                        ggggAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCAC‑CAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATggg                                                                                                                                                                                             >  1:173307/1‑250 (MQ=255)                               ccccaccatgtcccAGCCTCACCCCAATAAATAATCTTGGATACGGTTTCGTGCCAAGCGCCAGCCAACCGAAAAGAATCATGGCTGCCTCGCAC‑CAGCCTAAAATCAGCGGAAAAGAGATAAATTCCTTTTGCATAGCGTAAAGACCTAAAACCAAACCCATCCCCGTACCTCCAATACGCTCAAAGGCGCGAGGGACACCGTGACGCCAGAGCGAGATTGGCCCCATAATCACCACCATGGTGACCAGc                                                                                                                                                                                      <  2:531509/239‑1 (MQ=255)                                    accaTTGCCAGCGTCACCACAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCAGCGCAC‑CAGACTAACATCAGCGGTCACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTACAATCGAACCCAACACCGTACCGCCACAACGATCAAAGACGCGAGGGACAAAGTCACTCCAGAACGCGATTGGCCCAATAATCACCACCATGTTGACCCGCGGcc                                                                                                                                                                                  >  2:172578/4‑250 (MQ=255)                                           ccAGCTTCCCCCCAATCAATAAACCTTGATCCGTTTTCTTGCCCAGCGCCAGCCACCCCCAAAGGAACATGGCCGCCGCGCAC‑CAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGCCCTAAAAGCGAACCCACCACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCtt                                                                                                                                                                          <  1:103071/251‑1 (MQ=255)                                                         atcaatatacatgtattagctttCTTGCCCAGACCCAGCCAACCGGAAAGGAACAGGGCGGCCGCG‑ACACATCTTAACCTCATCGATCATGAGATTACCTCCAGTTGCAGAGCGATAAGCACTAAAATGGAACCCAACACCTTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACGCCAGAACGAGATTGGCCCCATAAGCACCACCATGGTGACCAGCGGCCAGGTGCTGTCCGGGATAGTaaa                                                                                                                                                            <  2:547041/231‑1 (MQ=255)                                                              tAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCAC‑CAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGc                                                                                                                                                       >  1:564774/1‑251 (MQ=255)                                                                      aaTACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCCC‑CAGACTAACATCAGCGGAAACGAGATTACCTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACCCCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAAt                                                                                                                                                <  1:551358/249‑1 (MQ=255)                                                                                      caccgcgagccaCCCGCAAAGGAACATGGCCGCCGCGCAC‑CAGACTAACACCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAACCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGa                                                                                                                               <  1:270587/244‑1 (MQ=255)                                                                                                                       cgcgCAC‑CAGACTAACATCAGCGGTAACGAGATTAACCCCAGTTGCAGAGCGATAAGACCAAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGc                                                                                              <  1:320268/251‑1 (MQ=255)                                                                                                                         cgcAC‑CAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATCAGACCTAAAATCGAACCCAACACCCTACCGCCCATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACAATGGCGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCAACATGCgg                                                                                            >  1:329719/1‑251 (MQ=255)                                                                                                                                  aCTAACATCAGCGGTAACAAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCg                                                                                    <  1:381417/251‑1 (MQ=255)                                                                                                                                                 aaCGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAg                                                                                                                                        <  1:470088/184‑1 (MQ=255)                                                                                                                                                 aaCGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAg                                                                                                                                        >  2:470088/1‑184 (MQ=255)                                                                                                                                                          aaCTCCAGTTGCAGAGAGATAAGACCTAAAATCGAACCCAACACCGGACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGAGTGGCACCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGGTAATGGGAAAAGTGAGCAGGAATGCCAGCGCGGCACGGGGAACATGCAAAATGCGGGCATGGCGGTGAACCCGGactatgaaagggat                                                            >  2:23662/1‑238 (MQ=255)                                                                                                                                                              ccatgtGCAGAGCGAAAAGACCTAAAACCGAACCCAACCCCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATACCCGGATTTCAAACGGGCTTAAt                                                        <  1:65017/246‑1 (MQ=255)                                                                                                                                                              ccAGTTGCAGGGCTATAAGACTTAAAAGCGAACCAAACACCTTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGTAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATACCCGGATTTCAAACGGGCTTAAt                                                        <  1:544366/251‑1 (MQ=255)                                                                                                                                                                aGTTGCAGCGCGATAATACCTAAAATCGATCCCAACACCGAACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAAGCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATACCCGGATTTCAAACGGGCTTAATGa                                                      <  1:424157/251‑1 (MQ=255)                                                                                                                                                                        agCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATACCCGGATTTCAAACGGGCTTAATGACTTATCgg                                              >  1:67961/1‑251 (MQ=255)                                                                                                                                                                               aGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCAACATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATACCCGGATTTCAAACGGGCTTAATGACTTATCGgcacgcac                                       <  1:208314/251‑1 (MQ=255)                                                                                                                                                                                          cGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTAACATGCACAATGCGGTAATGGCGGTATACCCGGATTTCAAACGGGCTTAATGACTTATCGGCACGCACGCAGAAACTcc                            <  1:454362/251‑1 (MQ=255)                                                                                                                                                                                                              cAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCCCAATGCGGTAATGGCGGTATACCCGGATTTCAAACGGGCTTAAATACTTATCAGCCCGCACGGCGACACTCCGTAATGAGATTAAAAacgcc        >  2:168216/1‑250 (MQ=255)                                                                                                                                                                                                                    gCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGCGTACCATGCACAATGCGGTAATGGCGGTATACCCGGGTTTCAAACGGGCTTAAGGAATTATCGGCAAGCACGCAGAAACTCCGGAATGAGGTTAAAAGCGCTAATAAc  >  1:102288/1‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                ACGCCGTATCAATTTCACCTGTCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCAC‑CAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATACCCGGATTTCAAACGGGCTTAATGACTTATCGGCACGCACGCAGAAACTCCGTAATGAGATTAAAAGCGCTCATAAC  >  NC_000913/2080168‑2080628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑