Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,064,616	C→T	A203T (GCT→ACT)	cobS ←	cobalamin 5'‑phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,064,616	0	C	T	97.6%	125.9 / NA	42	A203T (GCT→ACT)	cobS	cobalamin 5'‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (21/19);  minor base G (0/1);  total (22/20)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCGTCAATATGCCGTTGTGCACGCGACATAGCGGTAGAATCGATGGCTGGGATCGTATTCAGTAAATCGGCAAGTATTTGCATCTCACGTCTCTTTATAGCAGTGCCAGCAGGAAGACCAGTTCACCAAGCTCTATGGCTGCTCCCAGCGTATCGCCCGTTTGCCCGCCCAGCGTACGTTTGAGAAGCTGACCGAGGATGAAAATCGCCACCATCGTCACCACCATAGCAGCCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTTTGCCAATGAACACATTGCCAAGCCCTTCTTCCCGCGCATAACGATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCC  >  NC_000913/2064384‑2064857                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  cccGTCAATATGCCGTTGTGCACGCGACATAGCGGTAGAATCGATGGCTGGGATCGTATTCAGTAAATCGGCAAGTATTTGCATCTCACGTCTCTTTATAGCAGTGCCAGCAGGAAGACCAGTTCACCAAGCTCTATGGCTGCTCCCAGCGTATCGCCCGTTTGCCCGCCCCGCGTACGTTTGAGAAGCTGACCGAGGATGAAAATCGCCACCATCGTCACCACCATAGCAGTCACACCATGCATACCCgg                                                                                                                                                                                                                                 >  1:65029/1‑251 (MQ=255)  ccGTCAATATGCCTTCGTGCACGCGACATAGCGGTAGAATCGATGGCTGGGATCGTATTCAGTAAATCGGCAAGTATTTGCATCTCACGTCTCTTTATAGCAGTGCCAGCAGGAAGACCAGTTCTCCAAGCTCTATGGCTGCTCTCAGCGTATCGCCCGTGTGCCCGCGCATCTTACGTTTGAGAAGCTGACCGAGGATGAAAATCGCCACCATCGTCACCACCATAGCAGTCACACCATGCATACCCGGc                                                                                                                                                                                                                                <  1:359312/251‑1 (MQ=255)          atGCCGTTGTGCACGCGACATAGCGGTAGAATCGATGGCTGGGATCGTATTCAGTAAATCGGCAAGTATTTGCATCTCACGTCTCTTTATAGCAGTGCCAGCAGGAAGACCAGTTCACCAAGCTCTATGGCTGCTCCCAGCGTATCGCCCGTTTGCCCGCCCAGCGTACGTTTGAGAAGCTGACCGAGGATGAAAATCGCCACCATCGTCACCACCATAGCAGACACACACTGCATACCCGGCAATAATAc                                                                                                                                                                                                                        >  1:555907/1‑251 (MQ=255)              cGTTGTGCACGCGACTTATCTGTAGAATCGATGTCTGGGATCTTATTCAGTAAAGCGGCAAGTATTTGCAGTTCACGTCTCTTTATAGCAGTGCCATCATGAAGACCAGTGCCCCAAGCTTTATGGCGGCTCCCAGCGTATCGCCCGTTTGCCCTCCCAGCGTACTTTTGAGAAGCTGACCGAGGATGAAAATCGCCACCATCGTCCCCACCATAGCAGTCAAACCATGCATACCCGTTAATAATACGgca                                                                                                                                                                                                                    <  2:320887/251‑1 (MQ=255)                ttgtgcctgcgACATAGCGGTATAATCGATGGCTTGGATCGTATTCAGTAAATCGTCAAGTATTTGCATCTCACTTCTCTTTATAGCAGTGCCAGCATGAAGACCAGTTCACCAAGCTCTATGGCTGCTCCCAGCGTATCGCCCGTTTGCCCGCCCAGCGTACGTTTGAGAAGCTGACCGAGGATGAAAATCGCTAACATCTTCACCACCATAGCATTCACACCATGCATAACCGTCAATAATTCGgcag                                                                                                                                                                                                                   <  2:345834/242‑1 (MQ=255)                            cATAGCGGTAGAATCGATGGCTGGGATCGTATTCAGTAAATCGGCCAGTATTTACATCTCACGACTCTTTATAGCAGTGCCAGCAAGCAGACCAGTTCACCAAGCTCTATGGCTGCTCCCATCATATCGCCCGTTTGCCCGCCCAGCGTACGTTTGAGAAGCTGACCGAGGATGAAAATCGCCACCATCGTCACCACCATAGCAGTcaca                                                                                                                                                                                                                                               >  1:416969/1‑210 (MQ=255)                               aGCGGTAGAATCGATGGCTGGGATCGTATTCAGTAAATCGGCAAGTATTTGCATCTCTCGTCTCTTTATATCATTGCCAGCAGGAAGACCAGTTCACCAAGCTCTATGGCTGCTCCCAGCGTATCGCCCGTTTGCCCGCCCAGCGTACGTTTGAGAAGCTGACCGAGGATGAAAAGCGCCACCATCGTCACCACCATAGCAGTCACACCATGCATACCCGGCAATAATACGGCATCAAAAATAGCGGCTaa                                                                                                                                                                                                   <  1:208056/251‑1 (MQ=255)                                               gCTGGGATCGTATTCAGTAAATCGGCAAGTATTTGCATCTCACGTCTCTTTATAGCAGTGCCAGCAGGAAGACCAGTTCACCAAGCTCTATGGCTGCTCCCAGCGTATCGCCCGTTTGCCCGCCCAGCGTACGTTTGAGAAGCTGACCGAGGATGAAAATCGCCACCATCGTCACCACCATAGCAGTCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGc                                                                                                                                                                                     >  2:194311/1‑249 (MQ=255)                                                     aTCGTATTCAGTAAATCGGCAAGTATTTGCCTCTCACGTCTCTTTATAGCAGTGCCAGCAGGAAGACCAGTTCACCAAGCTCTATGGCTGCTCCCAGCGTATCGCCCGTTTGCCCGCCCAGCGTACGTTTGAGAAGCTGACCGAGGATGAAAATCGCCACCATCGTCACCACCATAGCAGTCACACCGAGCATAACCGGCAATAATACGGCAACACAAAAAGCGGCTCAGCCGaatggggagccagattgg                                                                                                                                                                             >  2:299428/1‑234 (MQ=255)                                                     aTCGTATTCAGTAAATCGGCAAGTATTTGCATCTCACGTCTCTTTATAGCAGTGCCAGCAGGAAGACCAGTTCACCAAGCTCTATGGCTGCTCCCAGCGTATCGCCCGTTTGCCCGCCCAGCGTACGTTTGAGAAGCTGACCGAGGATGAAAATCGCTACCATCGTCACCACCATAGCAGTCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGAAGCAGGTTTGt                                                                                                                                                                             >  1:489954/1‑251 (MQ=255)                                                                 aaaTCGGCAAGTATTTGCATCTCACGTCTCTTTATAGCAGTGCCAGCAGGAAGACCAGTTCACCAAGCTCTATGGCTGCTCCCAGCGTATCGCCCGTTTGCCCGCCCAGCGTACGTTTGAGAAGCTGACCGAGGATGAAAATCGCCACCATCGTCACCACCATAGCAGTCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGAc                                                                                                                                                                                       <  1:277938/229‑1 (MQ=255)                                                                 aaaTCGGCAAGTATTTGCATCTCACGTCTCTTTATAGCAGTGCCAGCAGGAAGACCAGTTCACCAAGCTCTATGGCTGCTCCCAGCGTATCGCCCGTTTGCCCGCCCAGCGTACGTTTGAGAAGCTGACCGAGGATGAAAATCGCCACCATCGTCACCACCATAGCAGTCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGAc                                                                                                                                                                                       >  2:277938/1‑229 (MQ=255)                                                                  aaTCGGCAAGTATTTGCATCTCACGTCTCTTTATAGCAGTGCCAGCAGGAAGACCAGTTCACCAAGCTCTATGGCTGCTCCCAGCGTATCGCCAGTTTGCCCGCCCAGCGTACGTTTGAGAAGCTGACCGAGGATGAAAATCGCCACCATCGTCACCACCATAGCAGTCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTTGCGCCCATCAAttt                                                                                                                                                                >  1:521868/1‑251 (MQ=255)                                                                    tCGGCAAGTATTTGCATCTCACGTCTCTTTATAGCAGTGCCAGCAGGAAGACCAGTTCACCAAGCTCTATGGCTGCTCCCAGCGTATCGCCCGTTTGCCCGCCCCGCGTACGTTTGAGAAGCTGACCGAGGATGAAAATCGCCACCATCGTCACCACCATAGCAGTCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGAGTGACGCAGGTTTGTCGCCCATCAAttttc                                                                                                                                                              >  1:268647/1‑250 (MQ=255)                                                                               ttGCATCTCACGTCTCTTTATAGCAGTGCCAGCAGGAAGACCAGTTCACCAAGCTCTATGGCTGCTCCCAGCGTATCGCCCGTTTGCCCGCCCAGCGTACGTTTGAGAAGCTGACCGAGGATGAAAATCGCCACCATCGTCACCACCATAGCAGTCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTTTGCCAATGAaca                                                                                                                                                    <  1:177164/250‑1 (MQ=255)                                                                                 gCATCTCACGGCTCTTTATAGCAGTGCCAGCAGGAAGACCAGTTCACCAAGCTCTATGTCTGCTCCCAGCGTAGCGCCCGTTGGCCCGCCCAGCTTCCGTGTGAGAAGCTGACCGGAGATAATAAACGCCACAAACGTCAACACCATAGCATTCACACCATGCATACCCGGCAATAATCCGGCAGCAAAAATAGCGACTAAGCCGAGTGTGCCGTACGTTTGTAGCGCATCAATCTTgtctttgaacaaa                                                                                                                                                  <  2:517046/250‑13 (MQ=255)                                                                                    tctcACGTCTCTTTATAGCAGTGCCAGCAGGAAGACCAGTTCACCAAGCTCTATGGCTGCTCCCAGCGTATCGCCCGTTTGCCCGCCCAGCGTACGTTTGAGAAGCTGACCGAGGATGAAAATCGCCACCATCGTCACCACCATAGCAGTCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTTTGCCAATGAACACATTGc                                                                                                                                              >  1:164406/1‑251 (MQ=255)                                                                                       aaCGTCGCTTTATAGCAGTGCCAGCAGGAAGACCAGTTCACCAAGCTCTATGGCTGCTCCCAGCGTAGCGCCCGTTTGCCCGCCCAGCGTTCGTTTGAGAAGCTGACCGAGGATGAAAAACGCCACCATCGTCACCACCATAGCAGTCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACTCAGGTGTGTCGCCCATCAATTTTTCCAATGAACACATTg                                                                                                                                               <  1:206275/246‑1 (MQ=255)                                                                                        aCGTCTCTTTATAGCAGTGCCAGCAGGAAGACCAGTTCACCAAGCTCTATGGCTGCTCCCAGCGTATCGCCCGTTTGCCCGCCCAGCGTACGTTTGAGAAGCTGACCGAGGATGAAAATCGCCACCATCGTCACCACCATAGCAGTCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTTTGCCAATGAACACATTGCCAAg                                                                                                                                          <  1:239967/251‑1 (MQ=255)                                                                                               tttATAGCAGTGCCAGCAGGAAGACCAGTTCACCAAGCTCTATGGCTGCTCCCAGCGTATCGCCCGTTTGCCCGCCCAGCGTACGTTTGAGAAGCTGACCGAGGATGAAAATCGCCACCATCGTCACCACCATAGCAGTCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTTTGCCAATGAACACATTGCCAAGCCcttct                                                                                                                                   >  1:521832/1‑251 (MQ=255)                                                                                                ttATAGCAGTGCCAGCAGGAAGACCAGTTCACCAAGCTCTATGGCTGCTCCCAGCGTATCGCCCGTTTGCCCGCCCAGCGTACGTTTGAGAAGCTGACCGAGGATGAAAATCGCCACCATCGTCACCACCATAGCAGTCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTTTGCCAATGAACACATTGCCAAGCCctt                                                                                                                                     <  1:174424/248‑1 (MQ=255)                                                                                                  ataGCAGTGCCAGCAGGAAGACCAGTTCACCAAGCTCTATGGCTGCTCCCAGCGTATCGCCCGTTTGCCCGCCCAGCGTACGTTTGAGAAGCTGACCGAGGATGAAAATCGCCACCATCGTCACCACCATAGCAGTCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTTTGCCAATGAACACATTGCCAAGCCCttcttc                                                                                                                                 <  1:404523/250‑1 (MQ=255)                                                                                                   taGCAGTGCCAGCAGGAAGACCAGTTCACCAAGCTCTATGGCTGCTCCCAGCGTATCGCCCGTTTGCCCGCCCAGCGTACGTTTGAGAAGCTGACCGAGGATGAAAATCGCCACCATCGTCACCACCATAGCAGTCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTTTACCAATGAACACCTTACCACGCCCTTCTTccc                                                                                                                               >  1:314717/1‑251 (MQ=255)                                                                                                    aGCAGTGCCAGCAGGAAGACCAGTTCACCAAGCTCTATGGCTGCTCCCAGCGTATCGCCCGTTTGCCCGCCCAGCGTACGTTTGAGAAGCTGACCGAGGATGAAAATCGCCACCATCGTCACCACCATAGCAGTCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTTTGCCAATGAACACATTGCCAAGCCCTTCTTCCcg                                                                                                                              <  1:406129/251‑1 (MQ=255)                                                                                                      cAGTGCCAGCAGGAAGACCAGTTCACCAAGCTCTATGGCTGCTCCCAGCGTATCGCCCGTTTGCCCGCCCAGCGTACGTTTGAGAAGCTGACCGAGGATGAAAATCGCCACCATCGTCACCACCATAGCAGTCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTTTGCCAATGAACACATTGCCAAGCCCTTCTTCCcgcg                                                                                                                            >  1:382422/1‑251 (MQ=255)                                                                                                                      aCCAGTTCACCAAGCTCTATGGCTGCTCCCAGCGTATCGCCCGTTTGCCCGCCCAGCGTACGTTTGAGAAGCTGACCGAGGATGAAAATCGCCACCATCGTCACCACCATAGCAGTCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTTTGCCAATGAACACATTGCCAAGCCCTTCTTCCCGCGCATAACGATGACGAt                                                                                                             >  1:113080/1‑250 (MQ=255)                                                                                                                         aGTTCACCAAGCTCTATGGCGGCTCCCAGCGTATCGCCCGTTTGCCCGCCCAGCGTACGTTTGAGAAGCTGACCGAGGATGAAAATCGCCACCATCGTCACCACCATAGCAGTCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTTTGCCAATGAACACATTGCCAAGCCCTTCTTCCCGCGCATAACGATGACGATACAt                                                                                                         <  2:113080/251‑1 (MQ=255)                                                                                                                                           gctgctCCCAGCGTTTCGCCCGTTTGCCCGCCCAGCGTACGTTTGAGAAGCTGACCGAGGATGAAAATCGCCACCATCGTCACCACCATAGCAGTCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTTTGCCAATGAACACATTGCCAAGCCCTTCTTCCCGCGCATAACGATGACGATACATCAATAATGCGGCAGTccc                                                                                       <  1:26406/251‑1 (MQ=255)                                                                                                                                                ccccAGCGTATCGCCCGTTTGCCCGCCCAGCGTACGTTTGAGAAGCTGACCGAGGATGAAAATCGCCACCATCGTCACCACCATAGCAGTCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTTTGCCAATGAACACATTGCCAAGCCCTTCTTCCCGCGCATAACGATGACGATACATCAATAATGCGGCAGTCCCGCGGc                                                                                  <  1:552352/250‑1 (MQ=255)                                                                                                                                                             cccGTTTGCCCGCCCAGCGTACGTTTGAGAAGCTGACCGAGGATGAAAATCGCCACCATCGTCACCACCATAGCAGTCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTTTGCCAATGAACACATTGCCAAGCCCTTCTTCCCGCGCATAACGATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACAtgc                                                                     >  1:349266/1‑251 (MQ=255)                                                                                                                                                               cGTTTGCCCGCCCAGCGTACGTTTGAGAAGCTGACCGAGGAGGAAAATCGCCACCATCGTCACCACCATAGCAGTCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTTTGCCAATGAACACATTGCCAAGCCCTTCTTCCCGCGCATAACGATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACAtgctg                                                                   <  2:369786/251‑1 (MQ=255)                                                                                                                                                                     cccgcccAGCGTACGTTTGAGAAGCTGACCGAGGATGAAAAGCGCCACCATCGTCACCACCATAGCAGTCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTTTGCCAATGAACACATTGCCAAGCCCTTCTTCCCGCGCATAACGAGGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCa                                                             <  2:456279/251‑1 (MQ=255)                                                                                                                                                                                           aGCTGACCGAGGATGAAAATCGCCACCATCGTCACCACCATAGCAGTCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTTTGCCAATGAACACATTGCCAAGCCCTTCTTCCCGCGCATAACGATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGcc                                       >  1:401429/1‑251 (MQ=255)                                                                                                                                                                                              tgaacGAGGATGAAAATCTCCACCATCGTCACCACCATAGCAGTCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAATCCGAGTTTAACGCAGGTTTGTCGCCCATCAATTTTGACAATGAACACATTGCCAAGCCCTTCTTCCCGCGCATAAAGATAACGATACATCAATAATGCGGCAGTACCGCGGCTGACCGCACATGGAGCCGGCAGCAATGccagaggtcggaaacgcgg                                    >  2:310629/5‑233 (MQ=255)                                                                                                                                                                                              tGACCGAGGATGAAAATCGCCACCATCGTCACCACCATAGCAGTCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTTTGCCAATGAACACATTGCCAAGCCCTTCTTCCCGCGCATAACGATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAg                                                   <  1:310629/236‑1 (MQ=255)                                                                                                                                                                                                  cGAGGATGAAAATCGCCACCAGCGTCACCACCATAGCAGTCACACCATGCATTCCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTTACGCAGGTTTGTCGCCCATCAATTTTGCCAATGAACACATTGCCAAGCCCTTCTTCCCGCGCATAACGAGGACGATACATCAATAATGCGGCATTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCaga                                <  2:65029/251‑1 (MQ=255)                                                                                                                                                                                                            aaTCGCCACCATCGTCACCACCATAGCAGTCACACCATGCATACCAGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTTTGCCAATGAACACATTGCCAAGCCCTTCTTCCCGCGCATAACGATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGc                      >  1:448756/1‑251 (MQ=255)                                                                                                                                                                                                              cggcgaccctcggcatcaccaTAGCAGGCACATCATTCATACCCGGCAAGAATTAGCGAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTATCCTATCAATTTTTCCAATGAACACTTTGCCAAGCCCTTCTTCCCGCGCATACCTATGACGATACATCAATATTGCGGCACTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGTTCGATTCGCCACGCAGAGCCAACTCGCtt                    <  2:489954/235‑1 (MQ=255)                                                                                                                                                                                                                   accaTCGTCACCAACATAGCAGTCACACCATGCCTACCCGGCAATAATACGGCACCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGCCGACCAACAATTTTGCCAATGAACACATTGCCAAGACCTTCTTCCCGCGAATAACGCTGACGATACATCAATAATGCGGCAGTCCCGCAGCAGACCGCCCATACCGCCGCCAGCGATGCAAGGATCGCTTCGCCACGCATAGcccacgcgcatcacac               >  2:106231/1‑237 (MQ=255)                                                                                                                                                                                                                         gTCACCACCATAGCAGTCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTTTGCCAATGAACACATTGCCAAGCCCTTCTTCCCGCGCATAACGATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAAt         >  1:316389/1‑251 (MQ=255)                                                                                                                                                                                                                                ccaTAGCAGTCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGCGTGCGACGCAGGTTTGTCGCCCATCAATTTTGCCAATGAACACATTGCCAAGCCCTTCTTCCCGCGCATAACGATGACGATACATCAATAATGCGGCCGTCCCGCGGCTGACAGCCCATGCCGACGCCAGCGCAGCACGGATCGATTCGCCAAGCAGCACCAACCCGCTTAACCCCAGAATCTTTGcc  >  2:228558/1‑251 (MQ=255)                                                                                                                                                                                                                                  aTAGCAGTCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTTTGCCAATGAACACATTGCCAAGCCCTTCTTCCCGCGCATAACGATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGGGCCAACTCGCTTAACACCAGAATCTTTGcc  >  1:332467/1‑249 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  CCCGTCAATATGCCGTTGTGCACGCGACATAGCGGTAGAATCGATGGCTGGGATCGTATTCAGTAAATCGGCAAGTATTTGCATCTCACGTCTCTTTATAGCAGTGCCAGCAGGAAGACCAGTTCACCAAGCTCTATGGCTGCTCCCAGCGTATCGCCCGTTTGCCCGCCCAGCGTACGTTTGAGAAGCTGACCGAGGATGAAAATCGCCACCATCGTCACCACCATAGCAGCCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTTTGCCAATGAACACATTGCCAAGCCCTTCTTCCCGCGCATAACGATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCC  >  NC_000913/2064384‑2064857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑