Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,064,246 C→T E74E (GAG→GAA)  cobT ← nicotinate‑nucleotide‑‑dimethylbenzimidazole phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,064,2460CT91.7% 136.0 / ‑1.1 48E74E (GAG→GAA) cobTnicotinate‑nucleotide‑‑dimethylbenzimidazole phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (1/3);  new base T (23/21);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 6.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACGTCCAAAAGCAACTTTTCAGCCTGACGGCGACTCATTGCCGGAGCTGAAGCAATATTGCCGCTACCTCGTGCGACACGCATGTTGATAAGCCCGGGGATAGGCTCAGCAGTATCAATACCAACATCAATTACGTGGACGTTAGCGCCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTTCCACGGGTCATATTTTCAGCCTGTATGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCCTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCATTCCTGCCAGTTGTATGGCAAGCACCTCCAGCTTTCCCAGGCTACCAACAGGTTTGAGTAACCCGTCAATATGCCGTTGTGCACGCGACATAGCGGTAGAATCGATGGCTGGGATCGTATTCAGTAAATCGGCAAGTATTTGCATCTCACGTCTCTTTATAGCAGTG  >  NC_000913/2064000‑2064489
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   
gACGTCCAAAAGCAACTTTTCAGCCTGACGGCGACTCATTGCCGGAGCTGAAGCAATATTGCCGCTACCTCGTGCGACACGCATGTTGATAAGCCCGGGGATAGGCTCAGCAGTATCAATACCAACATCAATTACGTGGACGTTAGCGCCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTTCCACGGGTCATATTTTCAGCCTGTATGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCTTccc                                                                                                                                                                                                                                                 >  1:345834/1‑251 (MQ=255)
gACGTCCAAAAGCAACTTTTCAGCCTGACGGCGAATCATTGCCGGAGCTGAAGCAATATTGCCGCTACCTCGTGCGACACGCATGTTGATAAGCCCGGGGATAGGCTCAGCAGTATCAATACCAACATCAATTACGTGGACGTTAGCGCCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTTCCACGGGTCATATTTTCAGCCTGTATGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCTTccc                                                                                                                                                                                                                                                 <  1:296751/251‑1 (MQ=255)
     ccAAAAGCAACTTTTCAGCCTGACGGCGACTCATTGCCGGATCTGAAGCAATATTGCCGCTACCTCGTGCGACACGCATGTTGATAAGCCCGGGGATAGGCTCAGCAGTATCAATACCAACATCAATTACGTGGACGTTAGCGCCCTCTTGTTCTGCCAGCACACACACGCCGGTTGTTCCACGGGTCATATTTTCAGCCTGTGTGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCTTCCCAGAc                                                                                                                                                                                                                                             <  2:293633/250‑1 (MQ=255)
      cAAAAGCAACTTTTCAGCCTGACGGCGACTCATTGCCGGAGCTGAAGCAATATTGCCGCTACCTCGTGCGACACGCATGTTGATAAGCCCGGGGATAGGCTCAGCAGTATCAATACCAACATCAATTACGTGGACGTTAGCGCCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTTCCACGGGTCATATTTTCAGCCTGTATGGCTGTCGCTTCTTTTGGAGAAATAGCGACCCCTTCCTCCCAGACGc                                                                                                                                                                                                                                           >  1:103198/1‑251 (MQ=255)
            cAACTTTTCAGCCTGACGGCGACTCATTGCCGGAGCTGAAGCAATATTGCCGCTACCTCGTGCGACACGCATGTTGATAAGCCCGGGGATAGTCTCAGCAGTATCAATACCAACATCAATTACGTGGACGTTAGCGCCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTTCCACGGGTCATATTTTCAGCCTGTATGGCTGTCACATCTTTTGGAGAAATAGCGACCCCTTCTTCCCAGACGCCGTGAt                                                                                                                                                                                                                                     <  2:349110/251‑1 (MQ=255)
                                gACTCATTGCCGGAGCTGAAGCAATATTGCCGCTACCTCGTGCGACACGCATGTTGATCAGCCCGGGGATAGGCTCAGCAGTATCAATACCAACATCAATTACGTGGCCGTTAGCGCCCGCTTGTTCTGCCAGCACACACAAGCCGGTTGTTCCACGGGTCATATTTTCAGCCTGTATGGCTGTCACTTCTTTTGGAGGAATAGCGACCCCTTCTTCCCAGACGCCGTGCTCGGCACACATAACCAGTAcc                                                                                                                                                                                                                 >  2:67810/1‑251 (MQ=255)
                                gACTCATTGCCGGAGCTGAAGCAATATTGCCGCTACCTCGTGCGACACGCATGTTGATAAGCCCGGGGATAGGCTCAGCAGTATCAATACCAACATCAATTACGTGGACGTTAGCGCCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTTCCACGGGTCATATTTTCAGCCTGTATGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCTTCCCAGACGCCGTGATCGGCACACATAACCAGTAcc                                                                                                                                                                                                                 <  1:63134/251‑1 (MQ=255)
                                gACTCATTGCCGGAGCTGAAGCAATATTGCCGCTACCTCGTGCGACACGCATGTTGATAAGCCCGGGGATAGGCTCAGCAGTATCAATACCAACATCAATTACGTGGACGTTAGCGCCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTTCCACGGGTCATATTTTCAGCCTGTATGGCTGTCACCTCTTTTGGAGAAATAGCGACCCCTTCTTCCCAGACGCCGTGATCGGCACACATAACCAGTAcc                                                                                                                                                                                                                 >  1:487815/1‑251 (MQ=255)
                                     aTTGCCGGAGCTGAAGCAATATTGCCGCTACCTCGTGCGACACGCATGTTGATAAGCCCGGGGATAGGCTCAGCAGTATCAATACCAACATCAATTACGTGGACGTTAGCGCCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTTCCACGGGTCATATTTTCAGCCTGTATGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCTTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCttt                                                                                                                                                                                                            <  1:243616/251‑1 (MQ=255)
                                      ttGCCGGAGCTGAAGCAATATTGCCGCTACCTCGTGCGACACGCATGTTGATAAGCCCGGGGATAGGCTCAGCAGTATCAATACCAACATCAATTACGTGGACGTTAGCGCCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTTCCACGGGTCATATTTTCAGCCTGTATGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCTTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCtttt                                                                                                                                                                                                           >  1:131622/1‑251 (MQ=255)
                                                  aaGCAATATTGCCGCTACCTCGTGCGACACGCATGTTGATAAGCCCGGGGATAGGCTCAGCAGTATCAATACCAACATCAATTACGTGGACGTTAGCGCCCGCTTGTTCTGCCAGCACACACCCGCCGGTTGTTCCACGGGTCATATTTTCAGCCTGTATGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCTTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGc                                                                                                                                                                                               >  1:206136/1‑251 (MQ=255)
                                                   aGCAATATTGCCGCTACCTCGTGCGACACGCATGTTGATAAGCCCGGGGATAGGCTCAGCAGTATCAATACCAACATCAATTACGTGGACGTTAGCGCCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTTCCACGGGTCATATTTTCAGCCTGTATGGCTGTCACTTCTTTTGGAGCAATAGCGACCCCTTCTTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCg                                                                                                                                                                                              >  2:241793/1‑251 (MQ=255)
                                                         agtGCCGCTACCCCGCGCGACACGCATGTTGAAAAGCCCGGGGATAGGCCCAGCATTATCAATGCCAACATCATTTTCGTGGACGTTAGCTCCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTTCCACGGGTCATATTTGCAGCCTGTATGTCTGTCACCTCTTTTGGAGAAATAGCGACCCCTTCTTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATg                                                                                                                                                                                         <  2:161130/248‑1 (MQ=255)
                                                                              ccGCATGGTGATAAGCCCGGGGATAGGCTTAGCAGTATCAATACCAACATCAATTTCGTGGACGTTAGCTCCCGCTTTTTCTGCCAGCACACACTCGCCGGTTGTTCCACGGGTCATATTTTCAGCCTGTATGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCTTCCCAGACGCCGTGATCGGTACACATAACCAGTACCGCGTGTTTGCCCACATGCGTTATGCCATTCAACCCCGGCTTTCCTg                                                                                                                                                                   <  2:338719/250‑1 (MQ=255)
                                                                               cGCATGTTGAGAAGACCGGGAATAGGCTCGGCAAAATCACTACCAACATCAATTACTTGGAAGTTAGCGCCCGCGTGTTCTGCCAGTACACACACGCCGATTGTTCAACGCGTAATATGTTGCGCCTGTATGGATATCACTTCTTTTGGAGAAATAGCGACCCCTCCTTCCCCGGCGCCGTGATCTGCACACATAACTAGCACCGCTTGTTTAACCACACACGGTCTGCCATCCCACCCCGGCATTCCTGc                                                                                                                                                                  >  2:206275/1‑251 (MQ=255)
                                                                                     ttGATAAGCCCGGGGATAGGCTCAGCAGTATCAATACCAACATCAATTACGTGGACGTTAGCGCCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTTCCACGGGTCATATTTACAGTCTGTATGGCTGTCACTTCTTTTGGAGTAATAACGACCCCTTCTTCCCCGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCTACATGCGGTATGCCATTCAACCCCGGCATTCATGCCAGttt                                                                                                                                                            >  2:177164/1‑250 (MQ=255)
                                                                                                   gATAGGCTCAGCAGTATCAATACCAACATCAATTACGTGGACGTTAGCGCCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTTCCACGGGTCATATTTTCAGCCTGTATGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCTTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCATTCCTGCCAGTTGTATGGCAAGCACCt                                                                                                                                              >  1:247386/1‑251 (MQ=255)
                                                                                                              cagTATCAATACCAACATCAATTACGTGGCCGTTAGCGCCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTTCCACGGGTCATATTTTCAGCCTGTATGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCTTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCgg                                                                                                                                                                                             >  1:244384/1‑193 (MQ=255)
                                                                                                                gTATCAATACCAACATCAATTACGTGGACGTTAGCGCCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTTCCACGGGTCATATTTTCAGCCTGTATGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCTTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCATTCCTGCCAGTTGTATGGCAAGCACCTCCAGCTTTCCCAg                                                                                                                                 <  1:76822/251‑1 (MQ=255)
                                                                                                                  aTCAATACCAACATCAATTACGTGGACGTTAGCGCCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTTCCACGGGTCATATTTTCAGCCTGTATGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCTTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCATTCCTGCCAGTTGTATGGCAAGCACCTCCAGCTTTCCCAGGc                                                                                                                               <  1:275132/251‑1 (MQ=255)
                                                                                                                    cAATACCAACATCAATTACGTGGACGTTAGCGCCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTTCCACGGGTCATATTTTCAGCCTGTATGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCTTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCATTCCTGCCAGTTGTATGGCAAGCACCTCCAGCTTTCCCAGGCTa                                                                                                                             <  1:265740/251‑1 (MQ=255)
                                                                                                                       tACCAACATCAATTACGTGGACGTTAGCGCCCGCTTGTTCTGCCAGCACACACACGACGGTTGTTCCACGGGTCATATTTTCAGCCTGTATGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCTTCCCAGACGCCGGTATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCATTCCTGCCAGTTGTATGGCAAGCACCTCCCGCTTTCCCAGGCTAcc                                                                                                                           >  2:140581/1‑250 (MQ=255)
                                                                                                                       tACCAACATCAATTACGGGGCCGTTAGCGGCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTTCCACGGGTCATATTTTCAGCCTGTATGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCTTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGGATGCCATTCAAACCCGGCATTCCTGCCCGTTGTATGGCAAGCACATCCAGCTTACCCAGGCTAcc                                                                                                                           >  2:23219/1‑250 (MQ=255)
                                                                                                                         ccAACATCAATTACGTGGACGTTAGCGCCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTTCCACGGGTCATATTTTCAGCCTGTATGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCTTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCATTCCTGCCAGTTGTATGGCAAGCACCTCCAGCTTTCCCAGGCTACCAAc                                                                                                                        <  1:241793/251‑1 (MQ=255)
                                                                                                                             cATCAATTACGTGGACGTTAGCGCCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTTCCACGGGTCATATTTTCAGCCTGTATGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCTTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCATTCCTGCCAGTTGTATGGCAAGCACCTCCAGCTTTCCCAGGCTACCAACAGGt                                                                                                                    >  1:422410/1‑251 (MQ=255)
                                                                                                                               tccaTTACGTGGACGTCGGCGCCCGCTTGTTCCGCCAGCACATACACGCCGGTTGTTCCACGGGTCATATTTTCAGCCTGTATGGCTTTCACTTCCTTTGGAGAAATAGAGACCCCATCTTCCCAGACGCCGCGATCGGCACACATATCCACTACCGCTATTTTGCCCACAAGCGTGATGCCATTCCACCCCGGCATTCCTGCCAGTTGTATGGCACGCCACTACAGTTTTCCCAGACTACCAACAGGttt                                                                                                                  >  2:113887/4‑251 (MQ=255)
                                                                                                                                    tACGTGGACGTTAGCGCCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTTCCACGGGTCATATTTTCAGCCTGTATGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCTTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCATTCCTGCCAGTTGTATGGCCAGAACCTCCAGCTTTCCCAGGCTTCACACAGGTTTgcgt                                                                                                              >  2:174424/1‑247 (MQ=255)
                                                                                                                                       gTGGACGTTAGCGCCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTTCCACGGGTCATATTTTCAGCCTGTATGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCTTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCATTCCTGCCAGTTGTATGGCAAGCACCTCCAGCTTTCCCAGGCTACCAACAGGTTTGAGGAAcc                                                                                                          >  2:221449/1‑251 (MQ=255)
                                                                                                                                         ggACGTTAGCGCCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTTCCACGGGTCATATTTTCAGCCTGTATGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCTTCCCAGACGCCGTGATCGGCACACATAACCAGTAACGCTTTTTTGCCCACATGCGGAATGCCATTCAACCCCGGAATTCCTGCAAGTTGTATGGCACGCACATCCAGCTTTCCCAGGCTCCCAAAAGGTTTGCGTAACCCg                                                                                                        >  2:404523/1‑251 (MQ=255)
                                                                                                                                            cGTTAGCGCCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTTCCACGGGTCATATTTTCAGCCTGTATGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCTTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCATTCCTGCCAGTTGTATGGCAAGCACCTCCAGCTTTCCCAGGCTACCAACAGGTTTGAGTAACCCGTc                                                                                                      >  2:239967/1‑250 (MQ=255)
                                                                                                                                                aGCGCCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTTCCACGGGTCATATTGTCAGCCTGTATGGCTGTCGCTTCTTTTGGAGAAATAGCGACCCCTTCCTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCATTCCTGCCAGTTGTATGGCACGCACCTCCAGCTTTCCCAGGCTACCAACAGGTTTGAGTA‑CCCGTCAatat                                                                                                 <  2:103198/250‑1 (MQ=255)
                                                                                                                                                   gcCCGCTTGTTCTGCCAGCACACTCACGCCGGTTGTTCCACGGGTCATATTTTCAGCCTGTATGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCTTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCATTCCTGCCAGTTGTATGGCAAGCACCTCCAACTTTCCCAGGCTACCAACAGGTTTGAGTAACCCGTCAATATGcc                                                                                              <  1:408954/251‑1 (MQ=255)
                                                                                                                                                   gcCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTTCCACGGGTCATATTTTCAGCCTGTATGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCTTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCATTCCTGCCAGTTGTATGGCAAGCACCTCCAGCTTTCCCAGGCTACCAACAGGTTTGAGTAACCCGTCAATATGcc                                                                                              <  1:390784/251‑1 (MQ=255)
                                                                                                                                                       gCTTGTTCTGCCAGCACACACACGCCGGTTGTTCCACGGTTCATATTTTCAGCCTGTATGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCTTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCATTCCTGCCAGTTGTATGGCAAGCACCTCCAGCTTTCCCAGGCTACCaa                                                                                                                         >  1:428988/1‑220 (MQ=255)
                                                                                                                                                                                       tCCACGGGTCATATTTTCAGCCTGTATGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCTTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCATTCCTGCCAGTTGTATGGCAAGCACCTCCAGCTTTCCCAGGCTACCAACAGGTTTGAGTAACCCGTCAATATGCCGTTGTGCACGCGACATAGCGGTAGAATCGATGGCt                                                           <  1:25070/250‑1 (MQ=255)
                                                                                                                                                                                           atggTCATATTTTCAGACTGTATGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCTTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCATTCCTGCCAGTGGTATGGCAAGCACCTCCAGCTTTCCCAGGCTACCAACAGGTTTGAGTAACCCGTCAATATGCCGTTGTGCACGCGACATAGCGGTAGAATCGATGGCTGGGAt                                                      <  2:422410/249‑1 (MQ=255)
                                                                                                                                                                                                     tttCATCCTGTATGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCTTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCATTCCTGCCAGTTGTATGGCAAGCACCTCCAGCTTTCCCAGGCTACCAACAGGTTTGAGTAACCCGTCAATATGCCGTTGTGCACGCGACATAGCGGTAGAATCGATGGCTGGGATCGTATTctg                                             <  2:159326/250‑3 (MQ=255)
                                                                                                                                                                                                              gTATGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCTTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCATTCCTGCCAGTTGTATGGCAAGCACCTCCAGCTTTCCCAGGCTACCAACAGGTTTGAGTAACCCGTCAATATGCCGTTGTGCACGCGACATAGCGGTAGAATCGATGGCTGGGATCGTATTCAGTAAATCGGc                                    <  1:23219/250‑1 (MQ=255)
                                                                                                                                                                                                              gTATGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCTTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCATTCCTGCCAGTTGTATGGCAAGCACCTCCAGCTTTCCCAGGCTACCAACAGGTTTGAGTAACCCGTCAATATGCCGTTGTGCACGCGACATAGCGGTAGAATCGATGGCTGGGATCGTATTCAGTAAATCGGCa                                   <  1:484060/251‑1 (MQ=255)
                                                                                                                                                                                                                aTGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCTTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCATTCCTGCCAGTTGTATGGCAAGCACCTCCAGCTTTCCCAGGCTACCAACAGGTTTGAGTAACCCGTCAATATGCCGTTGTGCACGCGACATAGCGGTAGAATCGATGGCTGGGATCGTATTCAGTAAATCGGCaa                                  <  1:488321/250‑1 (MQ=255)
                                                                                                                                                                                                                       tCACTTCTTTTGGAGAAATAGCGACCCCTTCTTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCATTCCTGCCAGTTGTATGGCAAGCACCTCCAGCTTTCCCAGGCTACCAACAGGTTTGAGTAACCCGTCAATATGCCGTTGTGCa                                                                                      >  1:430796/1‑191 (MQ=255)
                                                                                                                                                                                                                       tCACTTCTTTTGGAGAAATAGCGACCCCTTCTTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCATTCCTGCCAGTTGTATGGCAAGCACCTCCAGCTTTCCCAGGCTACCAACAGGTTTGAGTAACCCGTCAATATGCCGTTGTGCa                                                                                      <  2:430796/191‑1 (MQ=255)
                                                                                                                                                                                                                         cctgcttTTGGAGAAATTGCACCCCCTTCCTCCCAGACGACGTGATCTGCACCCCTAACCATTATCTCTTTTTTGCCCTCATGCGGTATGCCATTCAACCCCGGCATTCCTTCCTGTTGTATGTCAAGCACCTCCAGCTTTCCCAGGCTACTAACAGGTTTGAGTAACTCGTCAAGATGCCGTTGTGCACGCTACATAGCGGTAGAATCGATGGCTGGGATCGTATTCCTTAATTCTGCACGTATTTGctt                        <  2:80976/247‑3 (MQ=255)
                                                                                                                                                                                                                            tcttTTGGAGAAATAGCGACCCCTTCCTCCCAGACGCCGTGCACGGCACACATACCCAGTTCCGCTTTTTTGCTCACAGGCGTTATGCCATTCAACCCCGGCATGCCTGCCAGTTGTATGGCAAGCACCTCCAGCTTTCCCAGGCTACCAACAGGTTTGAGTAACCAGTCAATATGCCGTTGTGCACGCGACATAGCGGTAGAATCGATGGCTGGGATCGTATTCAGTACATCGGCAAGTATTTGCAtct                      <  2:296806/250‑1 (MQ=255)
                                                                                                                                                                                                                                    agaAATAGCGACCCCTTCTTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCCTTCCTGCCAGTTGTATGGCAAGCACCTCCAGCTTTCCCAGGCTACCAACAGGTTTGAGTAACCCGTCAATATGCCGTTGTGCACGCGACAAAGCGGTAGAATCGATGGCTGGGATCGTATTCAGTAAATCGTCAAGTATTTGCATCTCACGTctct             >  1:342692/1‑251 (MQ=255)
                                                                                                                                                                                                                                    agaAATAGCGACCCCTTCTTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCATTCCTGCCAGTTGTATGGCAAGCACCTCCAGCTTTCCCAGGCTACCAACAGGTTTGAGTAACCCGTCAATATGCCGTTGTGCACGCGACATAGCGGTAGAATCGATGGCTGGGATCGTATTCAGTAAATCGGCAAGTATTTGCATCTCACGTctct             <  1:67810/251‑1 (MQ=255)
                                                                                                                                                                                                                                      aaaTAGCGACCCCTTCTTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCATTCCTGCCAGTTGTATGGCAAGCACCTCCAGCTTTCCCAGGCTACCAACAGGTTTGAGTAACCCGTCAATATGCCGTTGTGCACGCGACATAGCGGTAGAATCGATGGCTGGGATCGTATTCAGTAAATCGGCAAGTATTTGCATCTCACGTCTCttt           >  1:517046/1‑251 (MQ=255)
                                                                                                                                                                                                                                               ccccTTCTTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCATTCCTGCCAGTTGTATGGCAAGCACCTCCAGCTTTCCCAGGCTACCAACAGGTTTGAGTAACCCGTCAATATGCCGTTGTGCACGCGACATAGCGGTAGAATCGATGGCTGGGATCGTATTCAGTAAATCGGCAAGTATTTGCATCTCACGTCTCTTTATAGCAGTg  >  1:306415/1‑251 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   
GACGTCCAAAAGCAACTTTTCAGCCTGACGGCGACTCATTGCCGGAGCTGAAGCAATATTGCCGCTACCTCGTGCGACACGCATGTTGATAAGCCCGGGGATAGGCTCAGCAGTATCAATACCAACATCAATTACGTGGACGTTAGCGCCCGCTTGTTCTGCCAGCACACACACGCCGGTTGTTCCACGGGTCATATTTTCAGCCTGTATGGCTGTCACTTCTTTTGGAGAAATAGCGACCCCTTCCTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCATTCCTGCCAGTTGTATGGCAAGCACCTCCAGCTTTCCCAGGCTACCAACAGGTTTGAGTAACCCGTCAATATGCCGTTGTGCACGCGACATAGCGGTAGAATCGATGGCTGGGATCGTATTCAGTAAATCGGCAAGTATTTGCATCTCACGTCTCTTTATAGCAGTG  >  NC_000913/2064000‑2064489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑