Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,041,411	G→A	G13S (GGT→AGT)	zinT →	metal‑binding protein ZinT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,041,411	0	G	A	100.0%	53.3 / NA	18	G13S (GGT→AGT)	zinT	metal‑binding protein ZinT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/8);  total (10/8)

AGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGAT‑GATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAAT  >  NC_000913/2041198‑2041652                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   aGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATgg                                                                                                                                                                                                               >  1:392896/1‑251 (MQ=255)                      caACCTCAAATCACCAAAATAATTTATTGTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTTCACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTGTACAAACTGGCTGGTGCTTTAAGTGTCTTTATTTTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAAcc                                                                                                                                                                                          <  2:540901/251‑1 (MQ=255)                                                               atagataAGCAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAACCTGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACAGCAAACCCTTAACAGAGGTCGAACACAAAGCTGCTAATGGTGTTTTtg                                                                                                                                                 >  2:469106/1‑251 (MQ=255)                                                                                       cATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGACCAAAAAGCTGCTAATGGTGTTTTTGAT‑GATGCCAATGAAAAAAACCGa                                                                                                                         >  2:111421/1‑250 (MQ=255)                                                                                                gTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGAT‑GATGCCAATGTACAAAACCGAACGCTCAGTg                                                                                                               >  1:75231/1‑251 (MQ=255)                                                                                                 tttCCTAAGAGTTGTTGAATTTTGCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAAATGTTTTGACGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGAGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCCTCACTCACACGGCAACACATTAACAGAGGTCGAACAAAAAGATACTAGTGGTGTTTTTGATAGA‑GCCAATGTAAAAAACCGAACGCTCAGTg                                                                                                               >  2:450041/1‑250 (MQ=255)                                                                                                                              tGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTATGGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTACCTTTTCGCATGGTCATCATTCACACGTCAAACCCTTACCAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTTAT‑GATGCCAATGTTAACAACAGAACGCTCAGTGCCTGGGATGAAGTCTGGCACTCCGATTATc                                                                                 >  2:211306/1‑251 (MQ=255)                                                                                                                                                         taCATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGAT‑GATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGACAACTTGAcc                                                      >  1:398336/1‑251 (MQ=255)                                                                                                                                                                 ctctGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGAT‑GATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAACGAGGCAAACTTGACCCCGTCttt                                              >  1:306865/1‑251 (MQ=255)                                                                                                                                                                    tGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATgg                                                                                                                                                         >  1:77092/1‑142 (MQ=255)                                                                                                                                                                    tGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATgg                                                                                                                                                         <  2:77092/142‑1 (MQ=255)                                                                                                                                                                    tGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGAT‑GATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACtt                                                          <  1:417476/236‑1 (MQ=255)                                                                                                                                                                    tGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACGCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTACTGGTGTTTTTGAT‑GATGCCACTGTACAAAACCGAACGCTCCTTGTCTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAACCtt                                                          >  2:417476/1‑236 (MQ=255)                                                                                                                                                                      gaggaAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGAT‑GATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCagaa                                         <  1:19180/251‑1 (MQ=255)                                                                                                                                                                                    ggCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGAT‑GATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAg                           <  1:217174/251‑1 (MQ=255)                                                                                                                                                                                       gtttCGTCTTTTCGAACTGGCTGGTGCTTTAAGTGTCTTTAAATTTCGCGCTCCTGCCTTTTCGCATGGGCCTCACTCACACGGCAAACCCTTAACAGAGGACGAACTAAAATCTGCTAATGGTTTTTGTGAT‑GTAGACAATGTACAAAACCTAACTCTCAGTCGCTGGGATGGAGTCTGGCAATCCGAGTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAACCGGATTCAGAta                        <  2:126013/249‑1 (MQ=255)                                                                                                                                                                                                  aCAAACTGGCTGTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGAT‑GATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACa             <  1:462311/251‑1 (MQ=255)                                                                                                                                                                                                             gTTGCTTTAAGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGAT‑GATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAAt  <  1:262392/251‑1 (MQ=255)                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   AGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGAT‑GATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAAT  >  NC_000913/2041198‑2041652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑