Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,040,433	C→A	A319D (GCC→GAC)	msrP →	protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,040,433	0	C	A	97.4%	109.4 / NA	39	A319D (GCC→GAC)	msrP	protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (22/16);  minor base T (0/1);  total (22/17)
Fisher's exact test for biased strand distribution p-value = 4.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

ACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGCCCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGTCGCACTGCACTGAAATTTTTGCTGGCGACCTTGTTAATCACCCCTCTGGCAC  >  NC_000913/2040191‑2040678                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      aCTGATTGGTCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGTGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGGCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTgg                                                                                                                                                                                                                                               <  1:250450/251‑1 (MQ=255)                           ttAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGTGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGAtt                                                                                                                                                                                                                       <  1:555536/249‑1 (MQ=255)                             aaaGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGTGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCg                                                                                                                                                                                                                   >  1:99029/1‑251 (MQ=255)                                                 cAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGTGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTc                                                                                                                                                                                               >  1:323249/1‑251 (MQ=255)                                                       tAAGCGGACCCGCTATCGTCCGCCAACCACCTGGAATATGGCAGTGCCTGTCTAATACGGTTTTTACGCCAACGTTAATCCGGATGTTGATCACCCGCGCTGGTCACAGGTTACCGAACGAGTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACTCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACa                                                                                                                                                                                          <  2:99029/250‑1 (MQ=255)                                                          gCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGTGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCa                                                                                                                                                                                       >  1:544756/1‑250 (MQ=255)                                                             gACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGTGCCTGACGACTACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATAGTGGCCTGGATTTGCGGGGGAATTTCTAAATGCGGCTGACAGCaaaa                                                                                                                                                                                    >  2:432140/1‑250 (MQ=255)                                                              aCCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGTGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACa                                                                                                                                                                                  <  1:209140/251‑1 (MQ=255)                                                                      gCGTCCGCCAACCACCTGGAATCTGGCAGTGCCTGACGAATTCGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACAt                                                                                                                                                                          <  2:50818/251‑1 (MQ=255)                                                                        gTCCGCCAACCACCTGGAATCTGGCAGTGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATgg                                                                                                                                                                        >  1:426073/1‑251 (MQ=255)                                                                                  caccTGGAATCTGGCAGTGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTAAAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGATGTATCGTGGCCTGGATTTGAGGGAGAATTTATAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGttt                                                                                                                                                              >  1:373601/1‑251 (MQ=255)                                                                                       ggAATCTGGCAGTGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCAACCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTg                                                                                                                                                         >  1:380870/1‑251 (MQ=255)                                                                                             tgacagtgcCTGACGAATAAGGTTTTTACGCCAACGTTATTCCGTATGTTGCTCACCCGCGATGGTCAAAGACTACCGCACGATTTATTGGTTCAGGCGGCATCCTCGATGTATAGCGCCGGCCAATTCTAATGTTTAATGGTTGAGCCGACCAGGTGGCATCGCTGTATCGTGGGCTGGCTGTGCGGGAGAATTTCTAACTACGTCTGCAAGCAACACAGGTGACATGGCTGAAGGttttcattcatgtt                                                                                                                                                   >  2:50129/8‑240 (MQ=255)                                                                                                cagtgcCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCTAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGGTTGCCTGCATCTTGcc                                                                                                                                                <  1:53395/247‑1 (MQ=255)                                                                                                   tgcCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGCTCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGAGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACCGCAAAACAGGAGACATGGCTGAAAGTTTGCCTGCATCTGGCCggg                                                                                                                                             >  2:37650/2‑250 (MQ=255)                                                                                                         aCGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGAtt                                                                                                                                                                                                                       <  2:418756/171‑1 (MQ=255)                                                                                                         aCGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGAtt                                                                                                                                                                                                                       >  1:418756/1‑171 (MQ=255)                                                                                                           gAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGcc                                                                                                                                     >  1:103219/1‑251 (MQ=255)                                                                                                                           ccAACGTTAATCCGTATGTTGCTCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCACCGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGTGGGAGAATTTCTAAATGCGTCTGACAGCAAACCAGGTGACATGGCTGCAAGTTTGCCTGCATCTTGCCGGATTGTTCCCGTTTCTCTGGCTGGTc                                                                                                                     >  2:7428/1‑251 (MQ=255)                                                                                                                            cAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCt                                                                                                                    >  1:384189/1‑251 (MQ=255)                                                                                                                               cGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTggg                                                                                                                 >  1:150082/1‑251 (MQ=255)                                                                                                                               cGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCCGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTTTTGCCGTTTCTCTGGCTGGTCTggg                                                                                                                 >  2:48122/1‑251 (MQ=255)                                                                                                                               cGTTAATCCGTAGGTTGATCACCCGCGCTGGTCACAGGCTTCCGAACGATTTATTTGTTCAGGCGGCATCCGCGATGTACAGCGCCAGCCAACGCTACGGTTTAATGGTGACGCCGTCCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCGGGCTGGTCTggg                                                                                                                 <  2:170716/251‑1 (MQ=255)                                                                                                                                gTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGTACGTTTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTACTGGTTACGCCGACCAGGTGGCATCGCTGTTTCGTGGCCTGGATTTGCGGGAGCATTTCTAAATGCGTCTGACAGCAAAACAGGTGACTGGGCTGAAAGTTTGCCTGCATATTGCCGGATTGTTTCCGTTTCTCTGTCTGGTCTGGGc                                                                                                                >  2:252231/1‑251 (MQ=255)                                                                                                                                  tAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTTCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCg                                                                                                               >  1:357494/1‑250 (MQ=255)                                                                                                                                                 tCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTCCAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAACCAGGTGACATGGCTGAAAGTTTGACTGCATCTTGCCGGATTGTTGACGTTTATCTGGCTGGTCTGGGCGATCAATCACGGAGGAc                                                                                               >  1:557094/1‑251 (MQ=255)                                                                                                                                                    cccGGGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCATGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGGCTGACAGCAAAACAGGTGACATGGCTGGAAGTTTGCCTGCATCTGGCCGGATTGTTGCCTTTGCTCTGGCTGTTCTTGGCGATCAATCGCGGTGGACt                                                                                              <  2:522135/249‑1 (MQ=255)                                                                                                                                                     ccGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCATCGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTgtg                                                                                           <  2:150082/251‑3 (MQ=255)                                                                                                                                                       ccgcTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTg                                                                                         <  2:544756/250‑1 (MQ=255)                                                                                                                                                        cgcTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGAATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCAGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGAGGACTGGGTGc                                                                                        >  1:189751/1‑251 (MQ=255)                                                                                                                                                                                          cAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGt                                                      <  1:37650/251‑1 (MQ=255)                                                                                                                                                                                           aGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGTc                                                     <  2:567235/251‑1 (MQ=255)                                                                                                                                                                                           aGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGTc                                                     <  1:305319/251‑1 (MQ=255)                                                                                                                                                                                                    ccTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGTCgcactgca                                             <  1:53172/250‑1 (MQ=255)                                                                                                                                                                                                     cTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCATGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTATACATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGTTTGTTGCCGTTTATCTGGCTGGTCTGGGCGGTACATCACGGTGGACTGGGGGCCGAGCCGGTGAAAGATATTAAGCAttagatcgggagagcgtcggg                                           >  2:131247/1‑232 (MQ=255)                                                                                                                                                                                                     cTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCAtt                                                              <  1:131247/232‑1 (MQ=255)                                                                                                                                                                                                         aTGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGTCGCACTGCACTGaa                                        >  1:267338/1‑250 (MQ=255)                                                                                                                                                                                                                   ccagccaACGCTACTGTTTAACGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGCTTTGCGGGAGAATTTCTCAATGCGTCTGACAGCAAAACCGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGCTTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCAAGGGGGACTGGGTGCCGCTCCGGTGAAAGATATTCATCATTTTACTTGTCTCAATGCACTGAAATTTTTGCTgg                             >  2:102181/1‑251 (MQ=255)                                                                                                                                                                                                                                       aTGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGTCGCACTGCACTGAAATTTTTGCTGGCGACCTTGTTAATCACCCct         <  1:278067/251‑1 (MQ=255)                                                                                                                                                                                                                                              cgccgaCCAGGTGGCATCGCTGTATCGTAGCCTAGATTTGCTAGAGAATTTCTCAATGCGTCTGACAGCAACACCGATGCCATGGCTGAAATTTTTCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGTCTGTTCTTGGCGATCAATCACTGTGGACTTTGTGCCGATCCGGTGAAAGATATTCCGCATTTTACTGGTCTCACTTCACTGCAATTTTTGCTTGCGACCTTGTTAATCACCCCTCTGGCAc  >  1:473924/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      ACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGCCCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGTCGCACTGCACTGAAATTTTTGCTGGCGACCTTGTTAATCACCCCTCTGGCAC  >  NC_000913/2040191‑2040678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑