Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,994,637	A→G	Y24H (TAC→CAC)	uvrC ←	UvrABC excision nuclease subunit C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,994,637	0	A	G	100.0%	140.9 / NA	40	Y24H (TAC→CAC)	uvrC	UvrABC excision nuclease subunit C
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/19);  total (21/19)

AAGATAAAAGGATATGATTTATCATCGCGTAGCAAAACGTTGTAACGCGGCTGATAGAGTTTGATGTAGTTGTGTTCCAGCAACAGCGCTTCGGTTTCTGTGTGAGTAACCGTTACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTACATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTG  >  NC_000913/1994390‑1994874                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                              aaGATAAAAGGATATGATTTATCATCGCGTAGCAAAACGTGTTAACGCGGCTGATAGAGTTTGATGTAGTTGTGTTCCAGCAACAGCGCTTCGGTTTCTGTGTGAGTAACCGTTACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCTGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCAt                                                                                                                                                                                                                                            <  2:187723/251‑1 (MQ=255)          gtatatGATTTATCATCGCGTAGCAAAACGTTGTAACGCGGCTGATAGAGTTTGATGTAGTTGTGTTCCAGCAACAGCGCTTCGGTTTCTGTGTGAGTAACCGTTACATCAATATGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAAc                                                                                                                                                                                                                                   <  1:563886/249‑1 (MQ=255)                    tATCATCGCGTAGCAAAACGTTGTAACGCGGCTGATAGAGTTTGATGTCGTGGTGTTCCAGCAACAGCGCTTCGGTTTCTGTGTGAGTAACCGTTACAACAATTTGCTGGATCTGGGCGACAAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGTAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCctggccgg                                                                                                                                                                                                                         >  1:418699/1‑248 (MQ=255)                          cgcgTAGCAAAACGTTGTAACGCGGCTGATAGAGTTTGATGTAGTTGTGTTCCAGCAACAGCGCTTCGGTTTCTGTGTGAGTAACCGTTACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGtt                                                                                                                                                                                                                   <  1:155456/251‑1 (MQ=255)                          cgcgTAGCAAAACGTTGTAACGCGGCTGATAGAGTTTGATGTAGTTGTGTTCCAGCAACAGCGCTTCGGTTTCTGTGTGAGTAACCGTTACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGtt                                                                                                                                                                                                                   <  1:452618/251‑1 (MQ=255)                            cgTAGCAAAACGTTGTAACGCGGCTGATAGAGTTTGATGTAGTTGTGTTCCAGCAACAGCGCTTCGGTTTCTGTGTGAGTAACCGTTACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTAc                                                                                                                                                                                                                 <  1:13063/251‑1 (MQ=255)                             gTAGCAAAACGTTGTAACGCGGCTGATAGAGTTTGATGTAGTTGTGTTCCAGCAACAGCGCTTCGGTTTCTGTGTGAGTAACCGTTACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACg                                                                                                                                                                                                                >  1:15809/1‑251 (MQ=255)                                                        agagTTTGATGTAGTTGTGTTCCAGCAACAGCGCTTCGGTTTCTGTGTGAGTAACCGTTACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAAc                                                                                                                                                                                     <  1:72413/251‑1 (MQ=255)                                                            tttGATGTAGTTGTGTTCCAGCAACAGCGCTTCGGATTATGTGTGAGTAACCGTTACATCAATTTGCTGGATCTGTGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGACAAGCCGTTTTTTCAGGTCTTTCGATTTGCCGACATAGATACCCGTCCAACCAGCATCGTGCATGCGTTAAACGCCTGGCTGGCTGGTTACGGTTTTTGAAAACGCTTTTGCGACAAACTGAt                                                                                                                                                                                 >  2:20100/1‑251 (MQ=255)                                                                       tgtgTTCCAGCAACAGCGCTTCGGTTTCTGTGTGAGTAACCGTTACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGa                                                                                                                                                                       <  1:337467/250‑1 (MQ=255)                                                                          gtTCCAGGAACAGCGTGTCGGTTTATGGGTGAGTTACCGTTCCAGTAATTTGCGGGACCTGGACGACCTGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCTTTTTTTCAGGTCTTTCGCTTTGCCTACATAGATATCTGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTGTTGAGTCAAACTGATCACTCACTGAcga                                                                                                                                                                    <  2:171885/250‑3 (MQ=255)                                                                          gtTCCAGCAACAGCGCTTCGGTTTCTGTGTGAGTAACCGTTACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACtt                                                                                                                                                                    <  2:181912/250‑1 (MQ=255)                                                                               aGCAACAGCGCTTCGGTTTCTGTGTGAGTAACCGTTACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGTTAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTTCGGCAAACTGGTACCTCACTGACTTgtaaat                                                                                                                                                              >  2:422820/1‑246 (MQ=255)                                                                                 cAACAGCGCTTCGGTTTCTGTGTGAGTAACCGTTACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGt                                                                                                                                                            >  1:161061/1‑251 (MQ=255)                                                                                              gTTTCTGTGTGAGTAACCGTTACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTacac                                                                                                                                               >  1:366846/1‑251 (MQ=255)                                                                                                   tgtgtgAGTAACCGTTACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGCAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGAcc                                                                                                                                          >  1:70891/1‑251 (MQ=255)                                                                                                                 ttACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGCCTTGATAATGTCTCCGCATTACACAGACCATGGCAACTTGcc                                                                                                                             >  2:442350/1‑250 (MQ=255)                                                                                                                                 gATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGATACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGATGCAGCTCAAc                                                                                                            >  2:283795/1‑251 (MQ=255)                                                                                                                                       ggCGACCCGCGCTTCGGTTTGGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGcc                                                                                                      <  2:15809/251‑1 (MQ=255)                                                                                                                                         cGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGCCAt                                                                                                    <  1:422820/251‑1 (MQ=255)                                                                                                                                          gACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGCAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGCCATAGATAACCGTACCACCAGCATCGTGCATGCGATACACGCCTGGCTGGCTAGTTACGGTCTTTAAAAACGCTTTTGCTTCATACTGATCACACCCTGACTTGATAATGTCTCAGCATTACAAAGACCATGGCGAATTGCCAGGAGGGTACGCTCAACATCGCCAtt                                                                                                   >  2:507098/1‑250 (MQ=255)                                                                                                                                          gACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCTTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACCGACCATGGCGAATTGCCAGGTGGGTCAGCTCAACATCGCCATg                                                                                                   >  2:52138/1‑251 (MQ=255)                                                                                                                                                                           gCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTa                                                                  <  1:125367/251‑1 (MQ=255)                                                                                                                                                                                   aaTAGCTGGGAAGCCGATTTTTCAGGGCTTTCGCTTAGCCGAAATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGATTTTGCGTCAAACTGATCACTCACTGACTTGATGATGTCTCCGCATTACACAGACCATGGCGAATAGCTCGGAGATTCAGATCAACATAGAAATGAATGTTACGTATACTGAACATaagaacgagatagatgttcc                                                          >  2:339615/1‑232 (MQ=255)                                                                                                                                                                                     aaGCTGGAAAGCCATTTTTTCAGGACTTTCGCTTTGCCGACATAGATAACCGTAACACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAACAACGCTTTTGCATCAAACTGCTCACTCACTGACTTGATAATGTCTCCGCATTACACAGAACATGGCGAATTGCAAGGTGAGTCAGCTCAACATCGCAATGAATGGTTAGTTTACTGAACATACGCTAGCGGTAGCTGTTCAcc                                                        >  2:336312/2‑251 (MQ=255)                                                                                                                                                                                        cTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTTCCAGTTGAGt                                                                                                                     <  2:374477/187‑1 (MQ=255)                                                                                                                                                                                        cTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGt                                                                                                                     >  1:374477/1‑187 (MQ=255)                                                                                                                                                                                             aagccttttgttcatgcttttCGCTTTGCCGACATATATATCCGTACCACCAGCATCGTGCATGTGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACGGATCACTTACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATTGCCATGAATGTTTAGTTTACTGAACCTACGAGAGCGGTAGCTGTTCACCGttt                                                    <  2:411610/229‑1 (MQ=255)                                                                                                                                                                                             aaGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGttt                                                    >  1:411610/1‑247 (MQ=255)                                                                                                                                                                                                ccGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACt                                             <  1:559620/251‑1 (MQ=255)                                                                                                                                                                                                        tCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTTGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTg                                      <  2:427014/250‑1 (MQ=255)                                                                                                                                                                                                               tttCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACAt                                                                              <  2:352408/203‑1 (MQ=255)                                                                                                                                                                                                               tttCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACAt                                                                              >  1:352408/1‑203 (MQ=255)                                                                                                                                                                                                                ttCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGtt                               <  1:549258/249‑1 (MQ=255)                                                                                                                                                                                                                 tCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTg                            >  1:85846/1‑251 (MQ=255)                                                                                                                                                                                                                      ttGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACCTACGATAACGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTg                            >  2:362116/1‑246 (MQ=255)                                                                                                                                                                                                                            aCATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTg                 >  1:292222/1‑251 (MQ=255)                                                                                                                                                                                                                                    aaCCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTg         >  1:457636/1‑251 (MQ=255)                                                                                                                                                                                                                                          cccaccaGCATCGTGCATGCGATAAACGCCTGGCTGGCGTGTTTCGTTTATTAAAAACGCTTTTGCGTCAAACTGTTCACTCACTGACGTGATAATGGCTCCGCATTACTCAGACCATGTCGAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGGTCTCCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCtt   <  2:557326/250‑1 (MQ=255)                                                                                                                                                                                                                                           ccaccaGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTg  >  1:337043/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                              AAGATAAAAGGATATGATTTATCATCGCGTAGCAAAACGTTGTAACGCGGCTGATAGAGTTTGATGTAGTTGTGTTCCAGCAACAGCGCTTCGGTTTCTGTGTGAGTAACCGTTACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTACATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTG  >  NC_000913/1994390‑1994874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑