Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,894,839	T→C	L12P (CTC→CCC)	pabB →	aminodeoxychorismate synthase subunit 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,894,839	0	T	C	100.0%	72.8 / NA	21	L12P (CTC→CCC)	pabB	aminodeoxychorismate synthase subunit 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/12);  total (9/12)

GGAGTGGTTGGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACC‑TGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGCTCCAGCAGGTGCTGGATC  >  NC_000913/1894594‑1895069                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                       ggAGTGGGTGGCACGCAGATCGTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCGCGACAGCTTGTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACC‑TGCAAACATTGTTAACGCCTGCTAAATGGTTGGCGCTAATGAGTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTCTACCCTGGc                                                                                                                                                                                                                                   <  2:453568/251‑1 (MQ=255)                       tctGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACC‑TGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAAttt                                                                                                                                                                                                               <  1:481785/249‑1 (MQ=255)                           ccaccaGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTTCCATCAGTTCCTGGATCCGCTCTACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACC‑TGCAAACATTGTTAACTCCTGCTAAATGGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGTCTTCAGGACGCCGCTGAATTTTATTtc                                                                                                                                                                                                         <  1:145771/251‑1 (MQ=255)                           ccaccaGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACC‑TGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTACTTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTtc                                                                                                                                                                                                         >  1:186332/1‑251 (MQ=255)                                aGCGCGATTTCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACC‑TGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTTTCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCg                                                                                                                                                                                                    <  1:359725/251‑1 (MQ=255)                                                cGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTACACC‑TGCAAACATTGTTACCTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGGCAGGCCTGATGAAGACGTTATCTCACGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCg                                                                                                                                                                                    >  2:84491/1‑251 (MQ=255)                                                               gtgCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACC‑TGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCGCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTa                                                                                                                                                                     <  1:149726/251‑1 (MQ=255)                                                                     tCAGTTCCTGGATCCGCTTGACAGCTTTTTGCTGCTGTTCATGTGTGAGTGAAGGTAAACC‑TGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGTCGTTATCTCCCGCTGTGATTACTTTTCCCTTGCGTCAGGACGCCGCTTAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACt                                                                                                                                                                 <  2:507798/249‑1 (MQ=255)                                                                                     cTCGACAGCTTATTGATGCTGTTCATGGGTGAGTGAAGGTAAACC‑TGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGCCGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACATGCCATGGGCGATGCTTTTACACTCCGGCTATGCCGttcatc                                                                                                                                               >  2:329853/1‑251 (MQ=255)                                                                                                      gctgggtattggTGAGTGAAG‑TAAACCCTGCAAACAGTGATAACTCCTTCTAAATTGTTGGCGCTAATTATTTCATGCTTCCCTGCACATAGCCATGAGAGTCAGGGCTGATGAAGACGTTATCTCCCGCTGTGTTTACTTTACCCGGGCGGCAGGACGCCGCTGAATTGTATTTCTCCCTCTTAACCCTCCGGCCGTGGGCGATGCGTTTACACTCCGGTGATGCCGAGCACCCGtatatactctttga                                                                                                                               <  2:335900/240‑11 (MQ=255)                                                                                                                       gaAGGTAAACC‑TGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCgccgagcc                                                                                                             <  1:514998/251‑1 (MQ=255)                                                                                                                            tAAACC‑TGCAAACATTGTTATCTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGTCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGAttt                                                                                                        >  2:159245/1‑251 (MQ=255)                                                                                                                                               tatcTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTc                                                                                       <  1:159245/247‑1 (MQ=255)                                                                                                                                                      tGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGAcgcc                                                                                                                                                                                                                        >  1:322241/1‑114 (MQ=255)                                                                                                                                                      tGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGAcgcc                                                                                                                                                                                                                        <  2:322241/114‑1 (MQ=255)                                                                                                                                                                              ttCATGCTACCCGTCACATAGCCAGTAGAGTAAGGCCTGATGAAGACGGTATCTCCCGCGGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGAGCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGaaa                                                        <  1:309621/250‑1 (MQ=255)                                                                                                                                                                                    cTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACt                                                                                          >  1:513144/1‑210 (MQ=255)                                                                                                                                                                                     tACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCCCTTTAACCACTTTCg                                                                                      >  2:126134/1‑213 (MQ=255)                                                                                                                                                                                     tACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCg                                                                                      <  1:126134/213‑1 (MQ=255)                                                                                                                                                                                         cGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACg                                             >  1:58061/1‑250 (MQ=255)                                                                                                                                                                                                               gaaCTGATGGAGACGGTATCTACCTCCGGGCTTACTTTACCATGGCTCCAGGACGCCGCTGAATTTTATTTCTCTCGCTTAACCCACCTGCCGAGGGCGATGCTTTTACACTCTGGCTCTGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTCAAGAAACCGTTGTTAGTGAAAGCGCAACACGCACAACGACCACTGATGCCCCGCTACAGGt                      >  2:73814/3‑251 (MQ=255)                                                                                                                                                                                                                                   ccccgttgttttttttttaccCTGGCGTCAGGACGCCGCTGATTTTTATTTCCCCCGTTTAAGCCACCTGCCGTGGGCGATGCTTTTGCACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCTCTACAGGTGCTCCAGCAGGTGCTGGATc  <  2:18123/236‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                       GGAGTGGTTGGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACC‑TGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGCTCCAGCAGGTGCTGGATC  >  NC_000913/1894594‑1895069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑