Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,878,855	C→T	A28V (GCT→GTT)	yoaK →	putative membrane protein YoaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,878,855	0	C	T	100.0%	46.2 / NA	17	A28V (GCT→GTT)	yoaK	putative membrane protein YoaK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/8);  total (9/8)

ATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGCTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTG‑TGGCGATTATTGTCGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAA‑GGCTGCCTGTGTGCAGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACAATCGCACCAATAACGGCAACCACGAAGCTGCCAAAATTG  >  NC_000913/1878612‑1879082                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                      atggtagAAGTCGTTTCTGAAACAACCGATGCATACAGGGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGAGTGGTGATAACATGCGAATCGGTTTTATTTTTCCCGTTGTAATCTTCATTACAGGGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGCCCCg                                                                                                                                                                                                                                <  2:176573/248‑1 (MQ=255)                                                       gATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTG‑TGGCGATTATTg                                                                                                                                                                         <  1:442948/251‑1 (MQ=255)                                                       gATATTGCAATGCATCAGGAGAAAAAACACAACCAGCAAACACCTTTTGTCGCGCATCCAGCGATACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTCTTGGCGGCTATGTTGCCCCGGGAGCATAAAAGTGAAAACAACCACGATTATTATGATTGGTG‑TTGCGattattt                                                                                                                                                                         >  2:565585/1‑250 (MQ=255)                                                                aTGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGACCCGGGAGCATAAAGATGACAAAAACAACGATTATTATGTTGGGTG‑TGGCGAATATTGTCGTACTct                                                                                                                                                                >  1:351748/1‑250 (MQ=255)                                                                       aGGAGAAAAAACACAACCAGAAAACACCTTTTGTCGCGCATCCAGCGCTACATTCCTGAGGCGTATTCTCATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGGCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTG‑TGGCGATTATTGTCGTACTCGGCACTGa                                                                                                                                                         <  2:345054/251‑1 (MQ=255)                                                                                 acacAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTG‑TGGCGATTATTGTCGTACTCGGCACTGAGCTGGGAtgg                                                                                                                                               >  1:133014/1‑251 (MQ=255)                                                                                              ccacCTTTGGTCGCGCATCCAGCGCTACATTCCTGAGGCGTAGTTAGAGTCTGTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCTGTCGTATTTTTAGCATGGTGTTTTATTGGCGGCTATGTTGCACCGGGAGTATAAAGATGAAAAAAACAACGATTATTATGATGGGTG‑TGGCTATTATTGACGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCAcc                                                                                                                                  <  1:93787/250‑1 (MQ=255)                                                                                                              aTCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTG‑TGGCGATTATTGTCGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCaa                                                                                                                   >  1:569736/1‑250 (MQ=255)                                                                                                                ccAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGCCCCGGGGGCATAAAAATGAAAAAAACAAAGCTTATTATGATGGGTG‑TGGCGAATAATGTCGTACTCGGCACTGAGCTGGGATGGGGGTAACGGCACCTCTAAAAAATAGCAAA‑gg                                                                                                               >  1:488243/1‑251 (MQ=255)                                                                                                                          aTTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTG‑TGGCGATTATTGTCGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAA‑GGCTGCCtgtgt                                                                                                     <  1:571138/251‑1 (MQ=255)                                                                                                                                                          gTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTCTTATGATGGGTG‑TGGCGATTATTGTCGTACTCGGCCCTGAGCTGGGATGGGGGTAACGTCACCTCTAAAAATTAGCAAA‑GGCTGCCTGTGTGCAGCCTTTGTTCAATTTAAGCGTTAACtttt                                                                     >  1:348629/1‑251 (MQ=255)                                                                                                                                                                                  tttttCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCCATGTTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGGTGGGGG‑AGGCGATTATTGTTGTAATAGGCACTGGGCTTGGATGTGGGTAACGCAACCTCTAAAAAAAAGAAAA‑GGCCGGCTGTGGGCTGCCTTTGTGCAAATTAAACGTTAACTTTTAATATTACTTTAGGTAAATaaaac                                             >  1:167782/1‑247 (MQ=255)                                                                                                                                                                                        cGGTTGTAATCTTCATTACAGCGGGCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTG‑TGGCGATTATTGTCGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAA‑GGCTGCCTGTGTGCAGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACAAt                                       <  1:100823/251‑1 (MQ=255)                                                                                                                                                                                         ggtggTAATCTTCATTACAGCGGCCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGCCCCGGGGGCATAAAAATGAAAAAAACAAAGATTATTATGGTGGGGGGTGCCG‑TTATTGTTGTACTCGGCACTGAGCTGGGAGGGTGGGAACGTTCCCTCTAAAAAAAAGCAAAAGGAT‑CCTGGGTGCAGCCTTTGTGCAATTTTAGCGTATACTTTTAAACTTCATGttaataaaaatcaagacaat                                       >  2:319186/5‑231 (MQ=255)                                                                                                                                                                                                    tCATTTCAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTG‑TGGCGATTATTGTCGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAA‑GGCTGCCTGTGTGCAGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACAATCGCACCAATa                             <  2:133014/249‑1 (MQ=255)                                                                                                                                                                                                     cATTAAAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGAGGCTATGTTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTG‑TGGCGATTATTGTCGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAA‑GGCTGCCTGTGTGCAGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACAATCGCACCAATAACg                          <  2:103739/251‑1 (MQ=255)                                                                                                                                                                                                                              tGGTTTTTTATTGGCGGCTATGTTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTG‑TGGCGATTATTGTCGTACTAGGCACTGAGCTGGGGTGGGGGTAACGTCACCTCTAAAAAATAGCAAA‑GGCTGCCTGTGTGCAGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACAATCGCACCAATAACGGCAACCACGAAGCGGCCAAAATTg  >  1:145810/1‑250 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                      ATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGCTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTG‑TGGCGATTATTGTCGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAA‑GGCTGCCTGTGTGCAGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACAATCGCACCAATAACGGCAACCACGAAGCTGCCAAAATTG  >  NC_000913/1878612‑1879082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑