Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,853,977 C→T M1M (ATG→ATA) † ydjE ← putative transporter YdjE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,853,9770CT92.0% 65.3 / ‑2.3 25M1M (ATG→ATA) †ydjEputative transporter YdjE
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (13/10);  total (14/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

CAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGTTATTATGTAATTAATATGACTAAAATCAGCTAACTG  >  NC_000913/1853751‑1854200
                                                                                                                                                                                                                                  |                                                                                                                                                                                                                               
caatAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTaaa                                                                                                                                                                                                         <  1:75754/248‑1 (MQ=255)
   tAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTaaaa                                                                                                                                                                                                        >  1:427857/1‑249 (MQ=255)
     aGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGt                                                                                                                                                                                                    >  1:519107/1‑251 (MQ=255)
         aGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGc                                                                                                                                                                                                 >  1:80434/1‑250 (MQ=255)
                  aaTAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGGGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGc                                                                                                                                                                                        >  2:129359/1‑250 (MQ=255)
                                     cGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCtt                                                                                                                                                                    <  1:557527/251‑1 (MQ=255)
                                      gAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCttt                                                                                                                                                                   >  2:282778/1‑251 (MQ=255)
                                        ggTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTGCTATTAGGTg                                                                                                                                                                                                                         <  2:369482/195‑1 (MQ=255)
                                        ggTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGAAGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTg                                                                                                                                                                                                                         >  1:369482/1‑195 (MQ=255)
                                                             attattTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGgcgc                                                                                                                                            >  1:165093/1‑251 (MQ=255)
                                                                               gCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGa                                                                                                                          <  1:351097/251‑1 (MQ=255)
                                                                                   aCCAGCTTTGCTAATACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTTCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACaa                                                                                                                       <  2:557262/250‑1 (MQ=255)
                                                                                                   cgacATTACCGGAGTAACTCAAAAACCCTTGTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGTCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTTCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCTTCATTTTATTTCAAGGCGCAGTAACAAATAATATTGACAACGTGTGCTTAAATTAcc                                                                                                      <  2:436102/251‑1 (MQ=255)
                                                                                                             ggAGTAAATCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAAGTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTg                                                                                            <  2:306951/251‑1 (MQ=255)
                                                                                                              gAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTgg                                                                                           <  1:282778/251‑1 (MQ=255)
                                                                                                                      cAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATattatt                                                                                   >  1:434145/1‑251 (MQ=255)
                                                                                                                                    ataaCAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATg                                                                     >  1:479904/1‑251 (MQ=255)
                                                                                                                                                  cTTATAATAGCAACTCTACGATAATGAAACCGGGCCAGCGACAAAAATTCCAGTCTTGCGCCAATTTAATCCTATTGTTCTATTAGGCGCCTCGGCTTTGCTACACAGGTAGCAATTGATGCAAATTCACTTGCCGCATCTTTATCCTCCTTTTATTTCAAGGGGAAGGAACAAATAATATTTACAACGTGTCCTTAAATTAAGCCACCAGCTGAATATTATTGTCTTTGCAAAATGCTATCCAcacagac                                                       >  2:230765/1‑245 (MQ=255)
                                                                                                                                                       aataCCAAATATACGCTAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCgg                                                  >  1:431322/1‑251 (MQ=255)
                                                                                                                                                                tataCGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATc                                         >  1:403791/1‑251 (MQ=255)
                                                                                                                                                                                   gCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCAGATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGTAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGTTATTATGTAATTAata                      <  2:519107/251‑1 (MQ=255)
                                                                                                                                                                                    ccAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGAAGCAATTGCGGCAAATTCACTTGCCGCATCTTTCTCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGAACCCGGTGATTTATCAGTTATTATGTAATTAatat                     >  2:322983/1‑251 (MQ=255)
                                                                                                                                                                                                ggTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCACTTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATACTATTGACAACGTGGGCTTAAATTACCACACCAGCTGGATATTATTGACTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGTTATTATGTAATTAATATGCCTAATATc           >  2:29344/1‑249 (MQ=255)
                                                                                                                                                                                                    cAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGTTATTATGTAATTAATATGACTAAAATCAGCTaa     <  1:129359/251‑1 (MQ=255)
                                                                                                                                                                                                          tGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGTTATTATGTAATTAATATGACTAAAATCAGCTAACTg  <  1:553133/248‑1 (MQ=255)
                                                                                                                                                                                                                                  |                                                                                                                                                                                                                               
CAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGTTATTATGTAATTAATATGACTAAAATCAGCTAACTG  >  NC_000913/1853751‑1854200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑