Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,828,654	C→T	G361R (GGG→AGG)	astD ←	aldehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,828,654	0	C	T	97.7%	118.0 / NA	45	G361R (GGG→AGG)	astD	aldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (32/11);  minor base G (1/0);  total (34/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGAATGGCGCGGTACTGGCAGCACCGGTAAGCGGTTTGTTCCAGTTAACAATCCCCGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC  >  NC_000913/1828408‑1828893                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                cgAATGGTGCGGTACTTGAAGCACCGGTAAGCGGTATGTTCCAGTTAACAATCCCCGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACCTCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGc                                                                                                                                                                                                                                             <  1:190481/251‑1 (MQ=255)         gcgctACTGGCAGCACCGGTAAGCGGTTTGTTCCAGTTAACAACCCCCGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCaa                                                                                                                                                                                                                                     <  2:85012/247‑1 (MQ=255)              aCTGGCAGCACCGGTAAGCGGTTTGTTCCAGTTAACAATCCCCGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCCTTTCCATGATCCCCGGCGTCAGCCACGATg                                                                                                                                                                                                                                >  2:106533/1‑251 (MQ=255)                               gCGGTTTGTTCCAGTTAACAATCCCCGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAg                                                                                                                                                                                                               <  1:533837/251‑1 (MQ=255)                                      gTTCCAGTTAACAATCCCCGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCgcggcg                                                                                                                                                                                                        <  1:550731/251‑1 (MQ=255)                                       ttCCAGCTAACAATCCCCGCCCGCGCCTCCAGCAACAGTTGATCGCACTTTTCCCGCTCGGGGGACACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTAGCCATACAAATCGCTTCATCGAAAGGATCATAACGCCAGCCGCGAAATAACGGTCCGAACAACTCTTCATCTGATCCGCCAGCAACACCTGTCATTTCAATGATCCTCGGCGAAAGCAACGATGTCCCGGCTTGCAAAAAgcctggccc                                                                                                                                                                                                       >  2:374294/1‑244 (MQ=255)                                        tCCAGTTAACAATCCCCGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTCATAAGCGCGgcgc                                                                                                                                                                                                       >  1:124321/1‑250 (MQ=255)                                                     ccccGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGCAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACATCTTCCTCTGGTACGCCAGCCACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTACTCAGCGCGGCGCAAGCAGGGgtcgtc                                                                                                                                                                                         >  2:218991/1‑251 (MQ=255)                                                          ccctcgccgcCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGTAAACCAGCCCGCAAGAGAGGCCGAAGCGAGTGTTATTCGCAATTCGAATCGCGTCATCGAAAGTATCATAACTCCAGACGCGCAATAACGGTCCGAACACCGCTTCATCTGGTACGCCAGCAACGCCTGTCATTGCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAAGAAGCTCGTCGCAAGCTGGGGTCTTCCGccc                                                                                                                                                                                    <  2:245499/247‑1 (MQ=255)                                                          cccgcGCCTCCAACAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGccc                                                                                                                                                                                    >  2:223458/1‑251 (MQ=255)                                                               gcCTCCAGCAACAGTTGATCGAACTTTTCCAGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGAGTTATTCGCCAGTCGACTCGCTTCATCGAAAGGATAATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATACGGTACGCAAGCAACGCCTGTACTTAAAATGATCCGCGCCGACATCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGACGTCCGCCCCTCGc                                                                                                                                                                               >  2:423504/1‑251 (MQ=255)                                                                     aGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATTGAAAGTATCATAACACCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGCTGACCCTGCTTTTAATAAGCGCGGCGCGAGAAGGGGCCGTCCGCCCATCGCtacc                                                                                                                                                                           >  2:220327/1‑246 (MQ=255)                                                                                        tttCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAacc                                                                                                                                                      >  1:173001/1‑251 (MQ=255)                                                                                           cccGCTCGGGGGACACCAGACCGCAAGCGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGAACGCCAGCAACGCCTGTCATTTCAATGCTCCTCGGCGTCAGCAACGCTGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCCCTTCCAGTTGCTGCCAAGAATTAaccacc                                                                                                                                                   >  2:188959/1‑251 (MQ=255)                                                                                                  gggggACACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGGCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACctgctg                                                                                                                                              >  2:174913/1‑249 (MQ=255)                                                                                                  gggggAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCtgtg                                                                                                                                            >  1:376369/1‑251 (MQ=255)                                                                                                    gggAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTTCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCg                                                                                                                                          <  1:59221/251‑1 (MQ=255)                                                                                                     ggAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGAGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGATGCCATGCAGTAACCACCTGCTGTGCgg                                                                                                                                         >  2:518347/1‑251 (MQ=255)                                                                                                                 gCAAGAGATGCCGAAGCGAGTGTTATTCGCCATTCGAAGCGCTTCATTGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGTTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGaaa                                                                                                                             <  2:461043/251‑1 (MQ=255)                                                                                                                             gAAGCGAGTGTTCTTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTACTAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCCGTAACCACCTGCTGTGCGGCCTGTTCAGGAATCAGCCCTCCa                                                                                                                  >  2:406004/1‑250 (MQ=255)                                                                                                                             gAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCa                                                                                                                  >  1:104487/1‑250 (MQ=255)                                                                                                                                   aGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGCTGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCCGAAATCAGCCCGCCAATaaccg                                                                                                           >  2:534328/1‑248 (MQ=255)                                                                                                                                   aGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATaac                                                                                                             >  2:390245/1‑248 (MQ=255)                                                                                                                                   aGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACg                                                                                                           >  1:172791/1‑251 (MQ=255)                                                                                                                                                aTTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTc                                                                                              >  1:307924/1‑251 (MQ=255)                                                                                                                                                   cGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCCGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCAGCGGTTCGTCATc                                                                                           >  2:427205/1‑251 (MQ=255)                                                                                                                                                          cTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTc                                                                                              >  1:217292/1‑241 (MQ=255)                                                                                                                                                            tCATCGAAAGTATCATAACGCCAGACGCGCAATACCGGCCCGAACCCCTCTTCATCTGGTCCGCCAGCAACGCCTGTCATTTCAATGACCCTCGGCGTCAGCAACGATGCCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGCCCCCCCACCGCTCCCAGTTGCTGCCATGCAGTACCCCCCTGCTGTGCGGCCTGTTCAAAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTg                                                                                    <  1:106533/249‑1 (MQ=255)                                                                                                                                                             catgaaaaGTATCATAACGCCAGCCGCGAAATAAGGGCCCGAACCCCTTTTCATCTGGTCCGCCAGCAACGCCTGTCATTTAAATGATCCTCGGCGTCAGCAACGATGCCCCTGCTTGTAAAAAGCGCGGCGCAAGCAGGGGTCGCCCCCCCATCGCTTCCAGTTGCTGCCAGGCAGTAACCACCTGCTGGGCGGCCTGTCAAGAAACCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGccc                                                                                 <  1:390245/246‑1 (MQ=255)                                                                                                                                                             cATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACctgc                                                                                                                                                >  1:407551/1‑188 (MQ=255)                                                                                                                                                                cGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCACGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTCACCACCTGCTGGGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGGTGCCCGGc                                                                              >  2:486709/1‑251 (MQ=255)                                                                                                                                                                 gAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAAACACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCg                                                                             >  1:206549/1‑251 (MQ=255)                                                                                                                                                                     gTATCATAACGCCAGACGCGCAATAACGGTCCGACCACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTACCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCCGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCACAGTTTCCCGGCGTTa                                                                          >  2:275592/1‑250 (MQ=255)                                                                                                                                                                      tATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGCTCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATACGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTTAGCGGCCTGTTCAGAACTCAGCCCGCCAATACACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCggtcat                                                                        >  2:282118/1‑246 (MQ=255)                                                                                                                                                                       aTCATAACGCCAGACGCCCAATAAGTGTCCGAACACCTCTTCATCGGGTCCGCCAGCAACGCCTGTCATTTAAAGGTTCCTCGGCGCCAGCAACGTTGCCCCTGTTTGTAATAAGCGCGGCGCAAGCGGGGTTCGCCCGCCCATCGCTTCCATTTGCTGCCATGAATTACCCCCCTGTTGTGCGGCTTGTTCAGAAACCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAAt                                                                        <  1:215144/250‑1 (MQ=255)                                                                                                                                                                         cATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCgc                                                                     >  1:422365/1‑251 (MQ=255)                                                                                                                                                                           tAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCgctg                                                                   >  1:353258/1‑251 (MQ=255)                                                                                                                                                                                                           ctctTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCAt                                                                                                                                                               >  1:60354/1‑127 (MQ=255)                                                                                                                                                                                                           ctctTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCAt                                                                                                                                                               <  2:60354/127‑1 (MQ=255)                                                                                                                                                                                                                     ggTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTgcgc                         >  1:304011/1‑251 (MQ=255)                                                                                                                                                                                                                      gTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGATTGAAATAAGCGCGGCGCAAGCAGGGGTCGTACGCCCACCGCTTCCAGTTGATGCCATGCAGAAACCAACGGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCAGCGGATCGTCATCCAAGTTGCACGGCGTTAATCGCTGGCGGAGGGCAACCAGACGAGGACGAAACGCACCGCCCTGCGcc                        >  1:533429/1‑251 (MQ=255)                                                                                                                                                                                                                            cAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCCTCGCCCTCCGCCCCGCtt                  >  2:191764/1‑251 (MQ=255)                                                                                                                                                                                                                                aaCGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTc               >  1:108771/1‑250 (MQ=255)                                                                                                                                                                                                                                 aCGCCTGTCATTTCAATAATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGAGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGATGCCCGGCGTTAATCGCTGGCTGAAGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCac             >  2:561101/1‑250 (MQ=255)                                                                                                                                                                                                                                            ttCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAAcggc  >  1:182862/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                CGAATGGCGCGGTACTGGCAGCACCGGTAAGCGGTTTGTTCCAGTTAACAATCCCCGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC  >  NC_000913/1828408‑1828893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑