Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,812,346	C→T	G263R (GGA→AGA)	ydjO ←	protein YdjO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,812,346	0	C	T	97.0%	106.6 / NA	33	G263R (GGA→AGA)	ydjO	protein YdjO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (12/20);  minor base G (1/0);  total (13/20)
Fisher's exact test for biased strand distribution p-value = 3.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTT‑ATGCTGGCAACGGTCCGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAG  >  NC_000913/1812101‑1812580                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                           ggtggtggtGCACCTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTT‑ATGCTGGCAACGGTCTGtttt                                                                                                                                                                                                                                       <  1:482417/251‑1 (MQ=255)       ggtgCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTT‑ATGCTGGCAACGGTCTGTTTTGtat                                                                                                                                                                                                                                   >  1:351907/1‑249 (MQ=255)            accTTTCGCCCCCCTGTTTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAAGTCTT‑ATGCTGGCAACGGTCTGTTTTGTATAGGGGcc                                                                                                                                                                                                                            <  2:351907/249‑1 (MQ=255)                               gtccgtctgggtgtggcccgtccattAACCTTGGGGGCGCTGTTAATTTCCGTTGGTCCGCTTATGTACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAAATGGCACGCCATTAAGTCTT‑ATGCTGGCAACGTTCTGTTTTGTATAGGGGCCGTTGCCTTTCTTTTTAAAt                                                                                                                                                                                                         <  2:564321/226‑1 (MQ=255)                                        gcGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTT‑ATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCAt                                                                                                                                                                                                  <  1:309314/249‑1 (MQ=255)                                         cGATGGGCCTGCCAGTAACCCTGGGGGCGCTGTTCATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGAGGCTCGATGAAAGCTGGCACGCTGCCCATCCAGTGGCTGAAGCAAACCGATAAAGACATTCTGGATGGCGAAGAGGCCGCCGCAATGGCACACCATTAATTCTTTAT‑ATGGAAACCGTATGTTTTGTATTGGAGGCGTTGCCTTAATTTTTaaaagatcactcac                                                                                                                                                                                               >  2:492202/1‑240 (MQ=255)                                          gATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCAAACCATTAATTCTT‑ATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTTAATGTTACATcgcg                                                                                                                                                                                              >  1:490136/1‑251 (MQ=255)                                                     cAGTAACCCTGGGGGCTCTTTTAATCTCCGTGGGACCGCTTATCGACATGGGCCGTACGGCGTTAACCGTTAGTGGCGCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTT‑ATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATAc                                                                                                                                                                                   <  2:141502/251‑1 (MQ=255)                                                      aGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCCGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTT‑ATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACt                                                                                                                                                                                  >  1:523836/1‑251 (MQ=255)                                                             cTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTT‑ATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATAATGACTCat                                                                                                                                                                           >  1:431882/1‑251 (MQ=255)                                                               ggtggCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACTCCATTAATTCTT‑ATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCat                                                                                                                                                                           <  1:377922/249‑1 (MQ=255)                                                                     gcTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCAAGCTGACCAGCCAGTGGCTGAAGCAAACCGCTAAAGCCATTCTGGATAGTGAAGACGACGCCGCACTGGCACACCCTTAATTCTT‑ATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATTGTCCATCGCGGCGATCAATCATGACTCATAATTaaa                                                                                                                                                                    >  2:188404/1‑250 (MQ=255)                                                                                ccGTTGAACCGCTTATCGACATGGGCCGTACGTCTTTAAACGTTAGTGGCTCGATGACAGCTGGCACTCTAACCAGCCAGTGGCTTAAGCAAACCTATAAAGCCATTCTGGATATCTAAGACGACGCCGAACTGGCACACCATTAATTCTT‑ATGCTGGCAACGGTCTGTTTTGTATAGGTGCCGTTGCCTTACTTTTTAAATTTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCa                                                                                                                                                          <  2:257907/249‑1 (MQ=255)                                                                                    tGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATTACAGCTGGCACGCTGCCCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGGTAGCGAAGAAGACGCCGGACAGGCACCCCATTTATTCTT‑ATGCTGGCAACGGGCGGTTTTTTATAAGGGCCGTTTCCTTACTTTTTAAATGTTCCCTCCCGGCGATCGATACTGACTCCAATTTaaaaaaaactgcatggaa                                                                                                                                                     >  2:308646/1‑235 (MQ=255)                                                                                                cGACTTGGGCCGTACGTGGTTAAACGTTAGTGGCCTGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAACCCGATAAAGTCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTT‑ATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCATCGAAAAAAATGTCCaa                                                                                                                                         <  2:490136/250‑1 (MQ=255)                                                                                                    aTGGGCCGTACGGCGTTAAACGTTAGGGGCTCGTTGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACCCCATTAATTCTT‑ATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGTCTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTAt                                                                                                                                    <  1:469075/251‑1 (MQ=255)                                                                                                      gggCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAACCCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTT‑ATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCa                                                                                                                                  <  1:132914/251‑1 (MQ=255)                                                                                                          cGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTT‑ATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATa                                                                                                                               >  1:321662/1‑250 (MQ=255)                                                                                                                    tAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTT‑ATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGc                                                                                                                     <  1:42369/250‑1 (MQ=255)                                                                                                                     aaatgTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTT‑ATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTACTGGCt                                                                                                                    <  1:179347/246‑1 (MQ=255)                                                                                                                     aaaCTTTAGTGGTTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTT‑ATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAAGACATTCCTGGCt                                                                                                                    <  2:283307/250‑1 (MQ=255)                                                                                                                           tAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTT‑ATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCaatga                                                                                                              <  1:536174/250‑1 (MQ=255)                                                                                                                               ggCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCACTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTT‑ATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGACtgtg                                                                                                         >  1:203077/1‑251 (MQ=255)                                                                                                                                gCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTT‑ATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACCTTCCTGGCTCAATGAATGTGc                                                                                                        >  1:89919/1‑251 (MQ=255)                                                                                                                                     aTGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTT‑ATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGt                                                                                                    >  1:231537/1‑250 (MQ=255)                                                                                                                                                    cggACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACTCCATTAATTCTT‑ATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTGTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTaaaa                                                                                    <  2:46763/249‑1 (MQ=255)                                                                                                                                                                                             ggATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTT‑ATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTGAACTATCTGGAAt                                            >  1:540095/1‑250 (MQ=255)                                                                                                                                                                                             ggATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTT‑ATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGATTTCTAAGTGAACTATCTGGAAt                                            >  1:445306/1‑250 (MQ=255)                                                                                                                                                                                               aTAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTT‑ATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACt                                         <  1:135390/251‑1 (MQ=255)                                                                                                                                                                                                    gAAGTCGACGCCGAACTGGCACACCATTAATTCTT‑ATGCTGGCAACGGTCTGTTTTGTATAGGGGCCCGTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGTCTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTATCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCataa                                    <  1:82112/251‑1 (MQ=255)                                                                                                                                                                                                                        acacCATTAATTCTT‑ATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACtt                <  1:162507/251‑1 (MQ=255)                                                                                                                                                                                                                         cacCATTAATTCTT‑ATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTc               <  1:561661/251‑1 (MQ=255)                                                                                                                                                                                                                                        aTGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAg  <  1:308646/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                           GGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTT‑ATGCTGGCAACGGTCCGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAG  >  NC_000913/1812101‑1812580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑