Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,743,164	C→T	E27K (GAG→AAG)	ribC ←	riboflavin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,743,164	0	C	T	100.0%	122.6 / NA	40	E27K (GAG→AAG)	ribC	riboflavin synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/20);  total (20/20)

AGCAGTGGTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGCCCTTACCAGTGAAGGGGTAAGAATGGCTATTTTTTCACTGGAGAATTAATAAATCCTCGCTACAATAGACTGAATTTCC  >  NC_000913/1742922‑1743382                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                           agcagTGGTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACAt                                                                                                                                                                                                                    <  1:130497/251‑1 (MQ=255)                       gACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACTCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTCACGTTTCTTTCATCAGTTCAAAACTGCCATGGTTGCCGTTACTTTCCGTCACTGTCAGGCAGCAACCGTTCTGCGCCACGGTAGCACCGTGTTCCAGGACGTCCAGCATGTTGTCGGTTACTTTCACCACCTGCTTACGAAAATTTgtattctt                                                                                                                                                                                              >  2:55837/1‑244 (MQ=255)                                tgtCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCg                                                                                                                                                                                      >  1:445173/1‑250 (MQ=255)                                    cGCCAATTTCATCACTGCATTTCGCCGCACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTATCGTAAATCGACAcc                                                                                                                                                                                 >  2:566520/1‑251 (MQ=255)                                     gCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACAcc                                                                                                                                                                                 >  1:482468/1‑250 (MQ=255)                                                caCTGAATTTCGCCGCACGAGCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGGTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGAGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGt                                                                                                                                                                      <  1:416047/250‑1 (MQ=255)                                                          cgccgcACGCTCAACGTTTACCCAAGCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACaa                                                                                                                                                           <  2:86957/251‑1 (MQ=255)                                                              gcacgatgaaCGTTTACCCAATCCCCCACTATTAAATCGCCAAGATTGGTAACGCGTATGGTTACTTTCATCAACTCACATCTGACATGGTTGCCGTTAATTTCCTGCACGGTCAGGCAGAAACCGGTATGCGCCACCGAAGCACCGGTGTCCAGGCCGTCCAGCATGTGGTCGGGTAATTTCACCTCATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCATTTTTGCGGTGCCCTGTACAAtacc                                                                                                                                                       <  2:199991/243‑4 (MQ=255)                                                                            tACCCAATCCCCCACTTTTAAATCGCCAAAATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAGCTGACATGGTTGCCGTTAATTTCCGTCGCGGTCAGGCAGCAACCGTTATGAGCAACGAAAGCACCGGTTTCCAGGCCGTCCATCATGTGATCGGGTAACTTCACCACATGCGTAAGAAAATTTGGTTTCTCGGCAATCACCACAATTTTTGCGGTGCCCTGTACAATCACCGTAAACATCCtt                                                                                                                                          >  1:394/1‑250 (MQ=255)                                                                              cccAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACa                                                                                                                                       <  1:378212/251‑1 (MQ=255)                                                                                      cccACTTTTAAATCGCCAAGCTTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAAGCCCCGTAAACATACTTACAACTCCTg                                                                                                                                >  2:384460/1‑250 (MQ=255)                                                                                               aaaTCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGtt                                                                                                                       >  1:88848/1‑250 (MQ=255)                                                                                                aaacGCCAAGATGGGTAATGCTTACCGGTTCTTTCAGCAGGTCAAATCTGACATGGTTGCCTGTAATTTCCGTCACGGTCAGGCAGCATCCGTTATGCGCCACGGAAGCACCGGTGTCCAGGCCTTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACTCCAGTTTTGCGGTGACCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTaa                                                                                                                     <  2:74828/248‑1 (MQ=255)                                                                                                        aGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCt                                                                                                             >  1:368766/1‑251 (MQ=255)                                                                                                          aTTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACGCCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGt                                                                                                           <  1:211497/251‑1 (MQ=255)                                                                                                           gtGGTAATGGATAACATTTCTTTCATGAGGTCAAAACTGTCATGGTTGCCGTTAAGTTCCGTCTCGTTCAGGCAGCAACCGTTATGCGCCACGGTAGCATCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCTCCACATGCGTACGAATATGTGGTTTCTCGGCAATCTACACCAGTTTTGCGGTGCCCTGTACAATACCCGTAAACATACTTACATCTTCGGAACTCATTTAAGTCATTTTGt                                                                                                           <  2:386913/249‑1 (MQ=255)                                                                                                             ggTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGCGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGCCATTCTGTTc                                                                                                         >  1:416074/1‑250 (MQ=255)                                                                                                               tAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACAt                                                                                                                                              >  1:120390/1‑211 (MQ=255)                                                                                                               tAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACAt                                                                                                                                              <  2:120390/211‑1 (MQ=255)                                                                                                               tAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGTAATCAGTTAAGACATTCTGTTCAGc                                                                                                      >  1:341823/1‑251 (MQ=255)                                                                                                                    cGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCACGTAAACATACTTACAACTCCTGACATCAGTTAAGACATTCTGTTCAGCACAAt                                                                                                 >  1:400290/1‑251 (MQ=255)                                                                                                                          gTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACaa                                                                                                  <  2:403155/244‑1 (MQ=255)                                                                                                                          gTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACaa                                                                                                  >  1:403155/1‑244 (MQ=255)                                                                                                                               tttcATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCCTGTGGTCGGGGAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACGTTCTGTTCAGCACAATAGCAGGTGGa                                                                                       >  2:390651/1‑250 (MQ=255)                                                                                                                                ttcATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGaa                                                                                      >  1:180490/1‑250 (MQ=255)                                                                                                                                      aGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGccc                                                                               <  2:180490/251‑1 (MQ=255)                                                                                                                                       ggTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGCCCt                                                                              <  1:55837/251‑1 (MQ=255)                                                                                                                                                  aCATGGGTGCCGTGAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTGCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGCCCTTACCAGTGAAg                                                                   <  2:286765/251‑1 (MQ=255)                                                                                                                                                             gTTAAGTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCTGACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTGTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGCCCTTACCAGTGAAGGGGTAAGAATg                                                        <  2:283109/251‑1 (MQ=255)                                                                                                                                                                    tCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGCCCTTACCAGTGAAGGGGTAAGAATGGCTAttt                                                 >  1:235910/1‑251 (MQ=255)                                                                                                                                                                         cacgtgcAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGTCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATGTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACAGTCTGTTCAGCACAATAGCAGTTGGAAAACGCCCTTACCAGTGAAGGGGTAAGAATGTCTATTTTTTc                                             <  2:350799/244‑1 (MQ=255)                                                                                                                                                                               cAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTGCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGCCCTTACCAGTGAAGGGGTAAGAATGGCTATTTTTTCACTGgag                                      <  1:390651/251‑1 (MQ=255)                                                                                                                                                                                 ggCAGCAACCGTTATGCGCAACGGAAGCACCGGTTGCCAGGCCGTCCAGCATGTGGTCGGGTACCTTCCCCACATGCGTACGCAACTTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGCACATTCCCCGTAAACATACTTACACCTCCTGAAATCTGTTAAGACATTTTGTTCAGAACAATCGCAGGTGGAAGACGCCCTTCCCACGCAAGGGGTAAGACTGTCCAttttttgagtgtggc                                     >  2:117251/1‑241 (MQ=255)                                                                                                                                                                                     gcaACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGCCCTTACCAGTGAAGGGGTAAGAATGGCTATTTTTTCACTGGAGAATta                                 >  1:137425/1‑250 (MQ=255)                                                                                                                                                                                          cGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTGTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACACCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGCCCTTACCAGTGAAGGGGTAAGAATGGCTATTTTTTCACTGGAGAATTAATAAAt                           <  1:384460/251‑1 (MQ=255)                                                                                                                                                                                            ttATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGCCCTTACCAGTGAAGGGGTAAGACTGGCTATTTTTTCACTGGAGAATTAATAAATcc                         >  1:550005/1‑251 (MQ=255)                                                                                                                                                                                            ttATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGCACGACAATTTGGTTTCTCGTCACTCGCCACCAGATTTGCGGTGCCCTGTACAATCCCCGTAAACATACTCACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGCCCTTACCAGTGAAGGTGTAAGCATGGCAATTTTTTCACTGAAGAATTAATAAATcc                         >  2:401585/1‑251 (MQ=255)                                                                                                                                                                                              aTGCGCCACGGAAGCACCGGGTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTTCACCACATGCGTACGAAAATTTGGTTGCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGCCCTTACCAGTGAAGGGGTAAGAATGGCTATTTTTTCACTGGAGAATTAATAAATCCTc                       <  1:365663/251‑1 (MQ=255)                                                                                                                                                                                                   ccACGGAAGTACCGGTTTCCAGGCCGTCCAGCATTCGGGAGGGTAACTTCACCACAGGCGTACTAAAATTTGGTCTCTCGTCAATCGACACCAGTGTTGCTGTGCCATGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCAAAATAGCTGGTGGAAAACGACCTTACCAGTGAAGGGGTAAGAATGGCTATTTTTTCACTGGAGAAGTAATAAATCCGCGCTAc                  <  2:416074/251‑1 (MQ=255)                                                                                                                                                                                                                    tCCAGGCCGTCCAGCATGTGGGCGGGTAACTTCCCCACATGCGTTCGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGGAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAATCGCCCTTACCAGTGAAGGGGTAAGAATGGCTATTTTTTCACTGGAGAATTAATAAATCCTCGCTACAATATACTGTATTTcc  <  2:559769/250‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                           AGCAGTGGTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAGACATTCTGTTCAGCACAATAGCAGGTGGAAAACGCCCTTACCAGTGAAGGGGTAAGAATGGCTATTTTTTCACTGGAGAATTAATAAATCCTCGCTACAATAGACTGAATTTCC  >  NC_000913/1742922‑1743382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑