Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,652,331	T→C	intergenic (+2346/+397)	ydfD → / ← ynfP	lysis protein/protein YnfP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,652,331	0	T	C	100.0%	52.5 / NA	17	intergenic (+2346/+397)	ydfD/ynfP	lysis protein/protein YnfP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/9);  total (8/9)

GGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAC‑CAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGA‑TGACATTAACGAGACTGAGCAAAGAGCATATCATTGAT‑GTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCATG  >  NC_000913/1652109‑1652574                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    ggAGTTCGCCATGGTGAGCTGTGTGCACTTGGATGGGAGTACATAGATCTGAAAAAAGGAACAATGATGATCAGGAGAAAATTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAC‑CAATCGGATCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGA‑TGACAATAACGAGACTGAGCAAAGAGCATATCAATGAT‑GTTCACCTCAGAGAGTATGGCAGTACACAAAAACa                                                                                                                                                                                                                         <  2:75298/251‑1 (MQ=255)           cTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAA‑CAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGA‑TGACATTAACGAGACTGAGCAAAAAGGCTATCATTGAT‑GTTTACCTCAGAGAGTATGGCAGAACAGAAAAAAAAAAAAGCAcc                                                                                                                                                                                                               >  1:165245/1‑251 (MQ=255)                      tgtgCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACACAA‑CAGGGCATTCATATTATTTAACCAACAATCCACGCTCTCCGGGGTCCGA‑GGACATTAAACAGACTGAAGAACAAGAAAATAATTTGT‑TTTCACCCCAACGGGAATGGCAGAACACAAAAACAAACATGCACCTTTGTTTTTc                                                                                                                                                                                                     >  2:118515/1‑250 (MQ=255)                           aCTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGTAAC‑CCATCGGGTCATTCATTTTATTTAGCCCGCAATCCACTCTCTCCGGAGTAAGAATG‑CATTAACGCGACTTAGCAAAGAGCATATCATTAATTGTTC‑CCTCCGCGCGTATAGCCGAACAACAAACCAAACATGCACCTTTTTTTTTTAACCTg                                                                                                                                                                                               >  1:63472/1‑251 (MQ=255)                            cTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAC‑CAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGA‑TGACATTAACGAGACTGAGCAAAGAGAAAATCATTGAT‑GTTCACCTCAGAGAGGATGGCAGAACAGAAAAACAAAAATGCAACTTTGTTTTTTAACCTg                                                                                                                                                                                               >  1:86646/1‑250 (MQ=255)                                                                            aggaatctAGCAAAAGACAGTTGCACTGTACCATAAACACAGGCGGTATC‑CAATCGGGTAATTCAGCTTATTAAGCCAGCAATCGACGCTCACCGGCGTCAGA‑TGACATTAACGAGACTGAGCAAAGAGCATATCAGTGAT‑GTTCGCCTCAGAGAGGATGGGAGAACAGAAAAACAAAAATGCACCTTTGTTGTTCAACCTGAAGTGTCAGCGAGAGTAAAAAAGTATGGTGACTATTTTACCGTTGagtc                                                                                                                                              <  2:441536/244‑4 (MQ=255)                                                                                            aCCGTTTCACGGTACCAAAAACACAGGCGGGAAC‑CAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGA‑TGACATTAACGAGACTGAGCAAAGAGCATATCATTGAT‑GTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTgg                                                                                                                              <  1:379017/251‑1 (MQ=255)                                                                                                           cAAAAACACAGGCGGGAAC‑CAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCGCCGGAGTCAGA‑TGACATTAACGAGACTGAGCAAAGAGCATATCATTGAT‑GTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCATCGATaaaa                                                                                                               <  2:165245/251‑1 (MQ=255)                                                                                                                       cGGGAAC‑CAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGA‑TGACATTAACGAGACTGAGCAAAGAGCATATCATTGAT‑GTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTc                                                                                                   >  1:75566/1‑251 (MQ=255)                                                                                                                        gggAAC‑CAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGA‑TGACATTAACGAGACTGAGCAAAGAGCATATCATTGAT‑GTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTcc                                                                                                  <  1:168632/251‑1 (MQ=255)                                                                                                                                        cattcatCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGA‑TGACATTAACGAGACTGAGCAAAGAGCATATCATTGAT‑GTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCat                                                                                   >  1:53107/1‑251 (MQ=255)                                                                                                                                                                   cGCTCTCCGGAGTCAGA‑TGACATTAACGGGACTGAGCAAAGAGCATATCATTGAT‑GTTCACCTCAGAGAGTATGGCAGAACAGAAACACAAAAATGCACCTTTTTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGATGAATCAATAAGGCAGATGTGGGACGCAGAGATAAAACGTGCCGGCCTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCctgct                                                        >  2:344926/1‑251 (MQ=255)                                                                                                                                                                                       aCATTAACGAGACTGAGCAAAGAGCATATCATTGAT‑GTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGc                                     <  1:161523/251‑1 (MQ=255)                                                                                                                                                                                           tAACGAGACTGAGCAAAGAGCATATCATTGAT‑GTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTaa                                  <  1:484841/250‑1 (MQ=255)                                                                                                                                                                                           tAACGAGACTGAGCAAAGAGCATATCAATGAT‑GTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGAAGATGAGGGACGCAGCGATCAAACATGCAGGAATCCGCCATCAAAAATCATATCAGTCGAGACATACTTAAACCTGCTGGTCGCTGACAGCTGATACTAAc                                 >  2:520321/1‑251 (MQ=255)                                                                                                                                                                                                 gaCTGAGCAAAGATCATATCATAGAT‑GTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCACACTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCAGCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCGAACCCgtca                           <  2:532815/251‑4 (MQ=255)                                                                                                                                                                                                                            gTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCATg  <  2:66012/250‑1 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    GGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAC‑CAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGA‑TGACATTAACGAGACTGAGCAAAGAGCATATCATTGAT‑GTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCATG  >  NC_000913/1652109‑1652574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑