Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,643,679	A→T	L209Q (CTG→CAG)	ydfU ←	protein YdfU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,643,679	0	A	T	98.1%	174.6 / NA	53	L209Q (CTG→CAG)	ydfU	protein YdfU
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (23/29);  minor base G (0/1);  total (23/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCAATCAGATGATGCGGATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCAGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCATGCTGTCAGATTTTGTTGAGCAAGCAGCTCCAGTGATTCCGAT  >  NC_000913/1643432‑1643918                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 aCCAATCAGATGATGCGGATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATGTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGct                                                                                                                                                                                                                                               <  1:553846/251‑1 (MQ=255)        aGATGATGCGGATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCgg                                                                                                                                                                                                                                        <  1:555255/251‑1 (MQ=255)              tgCGGATCGTGGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGCCGCTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGAGTTGTTTCAATATGCTGGTGGCGGTCTGCGCTCCCGGTACGAt                                                                                                                                                                                                                                  >  2:230643/1‑251 (MQ=255)               gCGGATCGTCGGCTGGCATACCGCAGCCCGCCCACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTCACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATg                                                                                                                                                                                                                                 >  1:224638/1‑251 (MQ=255)                 ggATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTc                                                                                                                                                                                                                               >  1:197024/1‑251 (MQ=255)                   aTCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGGTGTTTCAATAGGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGc                                                                                                                                                                                                                             <  1:162440/251‑1 (MQ=255)                      gtcgGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTCCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCCATATGCTGGGGGCGGTCTGATCTCCCGGTACGATGTCGCttt                                                                                                                                                                                                                          >  2:472059/1‑251 (MQ=255)                         ggCTGGCAGACCGCAGCAAGCACACGGCTGTGTCCTAACCCAACGTGTTTATTTCCCCTTAACCCAGCGGCGACGTTTAGGCCTCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACTGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTcgc                                                                                                                                                                                                                       <  1:275394/251‑1 (MQ=255)                            tGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGCTTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGGCGCTTTCGCGGt                                                                                                                                                                                                                    >  2:303127/1‑251 (MQ=255)                             ggCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGt                                                                                                                                                                                                                    <  1:154735/250‑1 (MQ=255)                               cATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTGTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTaca                                                                                                                                                                                                                 <  1:230643/251‑1 (MQ=255)                                              cctcGGCTGTGTCTTAACCCAACGTGTGTATTTCCCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGAtttttt                                                                                                                                                                                                  <  2:537842/248‑1 (MQ=255)                                                acGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTGTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGTTTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTcc                                                                                                                                                                                                >  1:196882/1‑251 (MQ=255)                                                                     gtgtgtATTTCTCCTTAACCCCGCGGCGCCGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGCTTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATCTGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTCCACCGGGCAGCTTTTTTCCGCTG‑TACATCCCAGCGCacgc                                                                                                                                                                          >  2:254962/1‑250 (MQ=255)                                                                     gtgtgtATTTCTCCGTAACCCAGCGGCGACGTTTAGGCAGCTTCATGCAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTGTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTTCACCGAGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAacga                                                                                                                                                                          <  2:263132/251‑1 (MQ=255)                                                                         gaaattCTCCTGAACCCAGCGGCGGCGTTTAGGCAGCTTCATGAAAGATTCCGGTGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGGTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAACGAcgc                                                                                                                                                                       <  2:230767/246‑1 (MQ=255)                                                                          tATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGAAACGGTGCAAGATTTTTTGTTCGTTGTTTCAATATGCTGTTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTTGCGGTACACCGAGCAGATTTTTTCCGCTGGTA‑TTCCCCGCGAacgcagcgg                                                                                                                                                                     >  2:374312/1‑245 (MQ=255)                                                                           aTTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAACGACGCGAt                                                                                                                                                                    <  1:571371/251‑1 (MQ=255)                                                                              tctcCTTAACCCAGCGGCGACGTTTAGGCAGCCTCATGAAAGAGTCCGGAGACTCTTGATCAACGGTGATGCTTACCACCGTCTTTTTCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAACGACGCGATAc                                                                                                                                                                  <  2:271693/250‑1 (MQ=255)                                                                                       cccAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTCCGATGTCGCTTTCGCGGTACACCGGGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAACGACACGagatcggaaggg                                                                                                                                                          >  2:470486/1‑238 (MQ=255)                                                                                       cccAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAACGAcgcg                                                                                                                                                                      <  1:470486/237‑1 (MQ=255)                                                                                                 aCGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTGTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTGTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTGTCGCGGTACTCCGAGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAACGACGCTATACAGCCTCAGGTAGTGCATcca                                                                                                                                              <  2:9206/251‑1 (MQ=255)                                                                                                      tAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCaccacc                                                                                                                                         >  1:375281/1‑251 (MQ=255)                                                                                                       atgcatcTTCATTAAAGAGTCCGGAGACTCTGGATCAACGGTGATGCTTATCACCGTCGTGTCCTGTGGTGATTTTTGTTGTTGGTGGGCGTAATGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAAGATGCTGGTTGCGGTCTGCTCTCCCGGCACGATGTCGCTTTCGCGGTACATCGAGCAGATTGTTGCCGCTGGTA‑ATCCCAGCGAACGACGCGATACAGTTTCAGGTAGTGCAGccaccccc                                                                                                                                         <  2:258628/244‑4 (MQ=255)                                                                                                        ggCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTg                                                                                                                                       <  1:33868/251‑1 (MQ=255)                                                                                                                   gAAAGATTCCGGAGACTCTGGATCAACGGTGATGCGTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCg                                                                                                                            <  1:318096/251‑1 (MQ=255)                                                                                                                               agaCTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGAt                                                                                                                 >  1:420001/1‑250 (MQ=255)                                                                                                                                         tCAACGGTGCTGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGAAAAGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCACGGTACGATGTCGCTTTCGCGGTACACCGAGCAGCTTTTTTCCGCTGGTA‑ATCCCAGCGACCGACGCGATACAGCCTCAGGTAGTGCATCCACCACCAGCATGCAGCCCGCCCACCAGGACAATTCAACCa                                                                                                       >  2:524747/1‑250 (MQ=255)                                                                                                                                             cGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAACGCCGCGATACAGCCTCAGGTAGTGCATCCACCCCCTGATTGCCGACCGCCCCCCAGGATAATTCAGCCAAAGa                                                                                                   >  2:548180/1‑250 (MQ=255)                                                                                                                                                    gCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTcc                                                                                            <  1:242117/250‑1 (MQ=255)                                                                                                                                                        acctccGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCt                                                                                        <  1:86867/246‑1 (MQ=255)                                                                                                                                                                       tgtgGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTAt                                                                        >  1:539710/1‑251 (MQ=255)                                                                                                                                                                          ggtgATTTTTGTTTCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTg                                                                      <  1:338887/250‑1 (MQ=255)                                                                                                                                                                            ggATTTTTGTGGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGGCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGt                                                                   <  2:6289/250‑1 (MQ=255)                                                                                                                                                                                ttttGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGAc                                                                <  1:303127/250‑1 (MQ=255)                                                                                                                                                                                      tgCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGa                                                          <  1:83390/250‑1 (MQ=255)                                                                                                                                                                                        cTGGTGGGAGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCACGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACAGCCCACCCGGATACTTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTCTTGCGTAACGGCTGACgg                                                       >  2:44170/1‑250 (MQ=255)                                                                                                                                                                                              ggCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCa                                                 <  1:70992/251‑1 (MQ=255)                                                                                                                                                                                                   aaGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCACTATGCTGGGGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTAGCGGTACACCGAGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAACGACGCGATACAGACTCAGGTAGTGCAGACCCCACCTGAGTGCAGACGGCCCACCAGACAAAATCAGCCAAAGATAATTCCCGCCCATTCGATCAGCCTATTGCGTGACGGCTGCCGTAAATCACCCAt                                            >  2:314544/1‑251 (MQ=255)                                                                                                                                                                                                   aaGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCaaat                                                                                                    <  2:458424/195‑2 (MQ=255)                                                                                                                                                                                                   aaGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAg                                                                                                    >  1:458424/1‑195 (MQ=255)                                                                                                                                                                                                   aaGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGAt                                                            <  1:329597/235‑1 (MQ=255)                                                                                                                                                                                                   aaGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTA‑ATACCAGCGACCGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTCCCGCTTCTTGCGTGACggctagaccggaaaagcgac                                            >  2:329597/1‑233 (MQ=255)                                                                                                                                                                                                   aaGGCAACGGTGCAAGATTGTTTGTGCGTTGTTTCAATATGCTGTTGGCGGTCTGCTCTCCCGGTACGATGTCGCATTCTCGGCACACCTAGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCCCCCa                                             <  2:224638/250‑1 (MQ=255)                                                                                                                                                                                                       cAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACAACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCATGCTg                                        >  1:219591/1‑251 (MQ=255)                                                                                                                                                                                                           ggTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGCCGTCAATACCCCATGCTGTCa                                     >  2:216747/1‑250 (MQ=255)                                                                                                                                                                                                               cAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGCTTTTTTCCGCTGGTA‑CTCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGAAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTACGTACCGGATGACGTCCATAACCCATGCTGTccggttt                                >  2:331330/1‑245 (MQ=255)                                                                                                                                                                                                                   aTTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCATGCTGTCAGATTttgtt                            <  1:374312/251‑1 (MQ=255)                                                                                                                                                                                                                     tttttGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCACTGGTA‑ATCCCAGCGGCCGACGCGATACAGCCTCAGGGAGTGCATCCACCACCTGATTGCAGCACGCCCACCGGGATAATTCCGCCACAGATCATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCACTGCTGGCAGATTttgttg                           >  2:250510/1‑250 (MQ=255)                                                                                                                                                                                                                                  tttCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCCCCTGATTGCAGACCGCCCACCAGGCTAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTCATTGAGTGACGGATGACGTCCATCACACATGTTGTCAGATTTTGTTGAGCCAGCCGCTcc              >  2:159004/1‑250 (MQ=255)                                                                                                                                                                                                                                     cAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCATGCTGTCAGATTTTGTTGAGCAAGCAGCTCCAGTg          <  1:472059/251‑1 (MQ=255)                                                                                                                                                                                                                                     cAATAGGCTGGTGGCGGTCGGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAACGCCGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGCCCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCATGCTGTCAGATTTTGTTGAGCAAGCAGCTCCAGTg          <  2:420001/251‑1 (MQ=255)                                                                                                                                                                                                                                              ggtggCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCATGCTGTCAGATTTTGTTGAGCAAGCAGCTCCAGTGATTCCGAt  <  1:44170/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 ACCAATCAGATGATGCGGATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCAGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTA‑ATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCATGCTGTCAGATTTTGTTGAGCAAGCAGCTCCAGTGATTCCGAT  >  NC_000913/1643432‑1643918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑